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An improved method for in vitro regeneration of common bean (Phaseolus vulgaris L.) Agrociencia
Quintero-Jiménez,Anareli; Espinosa-Huerta,Elsa; Acosta-Gallegos,J. Alberto; Guzmán-Maldonado,H. Salvador; Mora-Avilés,M. Alejandra.
In vitro regeneration of common bean (Phaseolus vulgaris L.) is a requirement for genetic transformation which involves induction and development to the whole plant. Success in regeneration of common bean from tissue organ culture has been achieved to some extent in the last years. However, genotype effects in regeneration response as well as efficiency and reproducibility have been a limiting factor. Embryos, from four different varieties, were excised from sterilized mature seeds and cultured in Murashige and Skoog (MS) or Gamborg (GM) media containing 6-benzylaminopurine (BAP) (10 mg-1) and adenine (A) (0 or 20 mg-1). Efficient regeneration was achieved when inducing formation of differentiation of cells like bud clusters at the internodal segment of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenine; Bud clusters; Embryonic axes; Gamborg medium; Organogenesis.
Ano: 2010 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000100005
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Fox grape cv. Bordô (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-Benzylaminopurine levels BABT
Carvalho,Dayse Cristina de; Silva,André Luís Lopes da; Schuck,Mariane Ruzza; Purcino,Marivel; Tanno,Guilherme Nakao; Biasi,Luiz Antonio.
The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordô and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1 were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 μM) in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordô had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arginine; Nodal segment; Glucose; Adenine; Glycine.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200004
Registros recuperados: 2
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