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A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D.
Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this...
Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf
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A modified Arcan test to analyze the behavior of composites and their assemblies under out-of-plane loadings ArchiMer
Cognard, J. Y.; Sohier, L.; Davies, Peter.
Composite materials are a key element in weight reduction strategies, so the analysis of the mechanical behavior of assemblies of composite modules is of great importance. Failure initiation in bonded assemblies involving composites is often associated with crack initiation in the adhesive or delamination of the composite plies close to the adhesive joint, caused by interlaminar or through-thickness stresses. Thus, the analysis of the behavior of composites and their assemblies under out-of-plane loadings is necessary in order to optimize such structures. However, few experimental devices are proposed in the literature and they often require thick composite specimens which are not representative of most industrial applications. This paper describes the use...
Tipo: Text Palavras-chave: Laminates; Adhesion; Stress concentrations; Mechanical testing.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00026/13740/11039.pdf
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A study of the non-linear behaviour of adhesively-bonded composite assemblies ArchiMer
Cognard, Jean Yves; Davies, Peter; Sohier, S; Creac' Hcadec, R.
The objective of this study is to define a reliable tool for dimensioning of adhesively bonded assemblies, particularly for marine and underwater applications. This paper presents experimental and numerical results, which describe the non-linear behaviour of an adhesive in a bonded assembly for various loadings. A modified Arcan fixture, well-suited for the study of the behaviour of bonded metal-metal assemblies, was developed in order to focus on the analysis of the behaviour of the adhesive in thin films. The edge effects are minimised by using an appropriate design of the substrates so that experimental results give reliable data. To analyze the kinematics of the deformation of the adhesive joint non-contact extensometry techniques were employed. Thus,...
Tipo: Text Palavras-chave: Non linear behaviour; Finite element; Arcan fixture; Marine structures; Composite; Adhesion.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2079.pdf
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Adhesion and biocides inactivation of Salmonella on stainless steel and polyethylene BJM
Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano.
The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022
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Adhesion and invasion of Clostridium perfringens type A into epithelial cells BJM
Llanco,Luis A.; Nakano,Viviane; Moraes,Claudia T.P. de; Piazza,Roxane M.F.; Avila-Campos,Mario J..
ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Invasion; Clostridium perfringens type A; Necrotic enteritis; TpeL gene.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400764
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Adhesion of Salmonella Enteritidis to stainless steel surfaces BJM
Oliveira,Kelly; Oliveira,Tereza; Teixeira,Pilar; Azeredo,Joana; Oliveira,Rosário.
Adhesion of microorganisms to food processing surfaces and the problems it causes are a matter of strong concern to the food industry. Contaminated food processing surfaces may act as potential sources of transmission of pathogens in food industry, catering and in the domestic environments. Several studies have shown that adhesion of bacteria to surfaces partly depends upon the nature of the inert surfaces and partly upon the bacterial surface properties. The aim of this study was to compare the adhesion of four different strains of Salmonella Enteritidis to stainless steel 304 (SS 304). The effect of surface hydrophobicity and surface elemental composition on the adhesion process was also analysed. Hydrophobicity was evaluated through contact angle...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Salmonella Enteritidis; Hydrophobicity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200026
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Adhesion of the human pathogen Sporothrix schenckii to several extracellular matrix proteins BJMBR
Lima,O.C.; Figueiredo,C.C.; Pereira,B.A.S.; Coelho,M.G.P.; Morandi,V.; Lopes-Bezerra,L.M..
The pathogenic fungus Sporothrix schenckii is the causative agent of sporotrichosis. This subcutaneous mycosis may disseminate in immunocompromised individuals and also affect several internal organs and tissues, most commonly the bone, joints and lung. Since adhesion is the first step involved with the dissemination of pathogens in the host, we have studied the interaction between S. schenckii and several extracellular matrix (ECM) proteins. The binding of two morphological phases of S. schenckii, yeast cells and conidia, to immobilized type II collagen, laminin, fibronectin, fibrinogen and thrombospondin was investigated. Poly (2-hydroxyethyl methacrylate) (poly-HEMA) was used as the negative control. Cell adhesion was assessed by ELISA with a rabbit...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sporothrix schenckii; Fibronectin; Laminin; Type II collagen; Adhesion.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500020
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Alternagin-C, a disintegrin-like protein from the venom of Bothrops alternatus, modulates alpha2ß1 integrin-mediated cell adhesion, migration and proliferation BJMBR
Selistre-de-Araujo,H.S.; Cominetti,M.R.; Terruggi,C.H.B.; Mariano-Oliveira,A.; De Freitas,M.S.; Crepin,M.; Figueiredo,C.C.; Morandi,V..
The alpha2ß1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Alternagin-C (ALT-C), a disintegrin-like protein purified from the venom of the Brazilian snake Bothrops alternatus, competitively interacts with the alpha2ß1 integrin, thereby inhibiting collagen binding. When immobilized in plate wells, ALT-C supports the adhesion of fibroblasts as well as of human vein endothelial cells (HUVEC) and does not detach cells previously bound to collagen I. ALT-C is a strong inducer of HUVEC proliferation in vitro. Gene expression analysis was done using an Affimetrix HU-95A probe array with probe sets of ~10,000 human genes. In human fibroblasts growing on collagen-coated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha2ß1 integrin; Disintegrin; Snake venom; Adhesion; Gene expression; Extracellular matrix.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000007
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Applications marines des composites a matrice metallique d'aluminium assembles par collage (MMC) ArchiMer
Davies, R; Ritchie, M; Trumper, R.
Metal matrix composites (MMC) offer a unique combination of properties that make this class of material ideally suited to the fabrication of components subjected to complex loads, such as marine structures. In many marine applications the dominant requirement is for a reduction in structural weight, and where failure occurs by elastic buckling stiffness is critical. MMC's encompass a very broad class of materials which may be subdivided into three main groups: particulate, whisker and continuous fibre reinforced metals. These materials offer improvements in stiffness and the strength to weight ratio over conventional alloys. However, the ability to construct efficient marine structures is constrained by the ability to join these materials both to...
Tipo: Text Palavras-chave: Adhesion; Adhesives; Ship technology; Composite materials; Traitement de surface; Collage; Structures marines; Composites à matrice métallique.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1079.pdf
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Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface BJM
Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036
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Assessment of hydrophobicity and roughness of stainless steel adhered by an isolate of Bacillus cereus from a dairy plant BJM
Bernardes,Patrícia Campos; Andrade,Nélio José de; Ferreira,Sukarno Olavo; Sá,João Paulo Natalino de; Araújo,Emiliane Andrade; Delatorre,Deyse Maria Zanom; Luiz,Lívia Maria Pinheiro.
The interaction between the surface of stainless steel and Bacillus cereus was studied in terms of the characteristics of interfacial interaction determined from the measurement of the contact angle of the surface of B. cereus and stainless steel in the presence or absence of B. cereus adherence. The microtopographies and the roughness of the surface of stainless steel and stainless steel adhered by B. cereus were evaluated with the help of atomic force microscopy and perfilometry. The strain of B. cereus studied was considered hydrophilic, whereas the stainless steel was considered hydrophobic. The adhesion was not thermodynamically favorable (ΔGadhesion > 0) between the stainless steel and the strain of B. cereus studied. Thus, the interaction between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrophobicity; Roughness; Bacillus cereus; Dairy plant; Adhesion.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400017
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Association of number of tandem repeats in two important adhesins in Mycoplasma hyopneumoniae Arq. Bras. Med. Vet. Zootec.
Santos,L. F. dos; Alvarez,J.; Perez,A.; Moreira,M. A. S.; Pieters,M..
RESUMODiversidade genética de Mycoplasma hyopneumoniae tem sido relatada em análise múltipla de repetições em tandem em número variável (MLVA). O objetivo deste estudo foi descrever a distribuição espacial e a heterogeneidade genética de tipos de M. hyopneumoniae no Brasil, bem como investigar a correlação entre regiões de repetição 1 (RR1) e 3 (RR3) de duas adesinas importantes (P97 e P146). Foram identificados 39 tipos de MLVA baseados no número de repetições em tandem em P97 RR1 e RR3 P146. A correlação negativa significativa (Spearman's rho = -0,26; P = 0,022) entre P97 RR1 e RR3 P146 foi observada, o que sugere um possível mecanismo compensatório que permitiria a bactéria manter a sua capacidade de adesão. Os resultados contribuem para compreender a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Adhesion; VNTR; MLVA; Swine; Enzootic pneumonia.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000501461
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Biomimetic Approaches for the Development of New Antifouling Solutions: Study of Incorporation of Macroalgae and Sponge Extracts for the Development of New Environmentally-Friendly Coatings ArchiMer
Sánchez-lozano, Ilse; Hernández-guerrero, Claudia Judith; Muñoz-ochoa, Mauricio; Hellio, Claire.
Biofouling causes major economic losses in the maritime industry. In our site study, the Bay of La Paz (Gulf of California), biofouling on immersed structures is a major problem and is treated mostly with copper-based antifouling paints. Due to the known environmental effect of such treatments, the search for environmentally friendly alternatives in this zone of high biodiversity is a priority to ensure the conservation and protection of species. The aim of this work was to link chemical ecology to marine biotechnology: indeed, the natural defense of macroalgae and sponge was evaluated against biofoulers (biofilm and macrofoulers) from the same geographical zone, and some coatings formulation was done for field assays. Our approach combines in vitro and...
Tipo: Text Palavras-chave: Adhesion; Algae; Antifouling; Biofilm; Extracts; Field assays; Sponges; Haliclona caerulea; Laurencia gardneri; Sargassum horridum; Ircinia sp.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00589/70074/68047.pdf
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CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell BJMBR
Aguiar,D.P.; Coelho-Aguiar,J.M.; Abreu,J.G..
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004
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Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells International Journal of Morphology
Chiapparrone,María L; Soto,Pedro; Catena,María.
Campylobacter fetus is extracellular bacteria of the genital tract of cattle. They cause infertility and abortion, but there is no documented information on the susceptibility of bovine sperm cells to this bacteria. The aim of this present work was to study the effects provoked by Campylobacter fetus subsp. venerealis when in interaction with bovine sperm cells. The bovine spermatozoa were obtained frozen bovine semen pooled from uninfected bulls, and were exposed to living campylobacter over different periods of time. Light microscopy and scanning electron microscopy first revealed a tropism, then a close proximity followed by tight adhesion between these two different cells. A decrease in the spermatozoa motility was observed. Motile bacteria were...
Tipo: Journal article Palavras-chave: Adhesion; Campylobacter fetus subsp. venerealis; Sperm cells.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400040
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Curli fimbria: an Escherichia coli adhesin associated with human cystitis BJM
Cordeiro,Melina Aparecida; Werle,Catierine Hirsch; Milanez,Guilherme Paier; Yano,Tomomasa.
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curli fimbria; Adhesion; Escherichia coli; UPEC.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200414
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Development of alginate hydrogels active against adhesion of microalgae ArchiMer
Nassif, L. Abi; Rioual, S.; Trepos, Rozenn; Fauchon, M.; Farah, W.; Hellio, C.; Abboud, M.; Lescop, B..
Microorganisms have the ability to settle on nearly all man-made surfaces in contact with seawater and subsequently to form biofilm. Biofilms control and removal is necessary in the sectors of maritime transport, energy... In this work, we present the development of new pure calcium, zinc or copper alginate, but also mixed Ca/Cu and Ca/Zn alginate hydrogels. These materials have been evaluated for their potential inhibition of adhesion of two key biofilm-forming microalgae Halamphora coffeaeformis and Cylindrotheca closterium). All the tested materials have presented high adhesion inhibition about 80%). Copper-base materials present a high toxicity against H. coffeaeformis. Pure zinc alginate is also toxic for this strain. However, the addition of calcium...
Tipo: Text Palavras-chave: Biofilm; Adhesion; Alginate; Zinc; Biomaterials; Microalgae.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00605/71671/70168.pdf
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Development of an improved adhesive test method for composite assembly design ArchiMer
Cognard, Jean Yves; Davies, Peter; Gineste, B; Sohier, L.
This study is concerned with improving performance of composite structures through the use of adhesive bonding, particularly for marine and underwater applications. Some preliminary results from tests on simple composite/composite assemblies are given first. Difficulty in modelling the failure of even these simple joints highlighted the need for more reliable constituent input data. The first objective was to define an experimental methodology enabling the adhesives of interest to be characterised up to failure. A metal metal assembly was considered, in order to concentrate on the analysis of the behaviour of thin adhesive films. The aims were to characterise the adhesive, analyse its non-linear behaviour, and study the influence of parameters such as film...
Tipo: Text Palavras-chave: Finite element analysis; Adhesion.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-765.pdf
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Effect of 2 % Chlorhexidine on Dentin Shear Bond Strength of Different Adhesive Systems: A 6 Months Evaluation International Journal of Morphology
Bravo,Cristián; Sampaio,Camila S; Hirata,Ronaldo; Puppin-Rontani,Regina M; Mayoral,Juan Ricardo; Giner,Lluis.
This study evaluated the effect of chlorhexidine dentin treatment on shear bond strength (SBS) of adhesive systems after different storages. The work included 144 third molars that had their dentin exposed and were divided in 6 groups: G1 (ASB+CHX: Adper Scotchbond 1XT + chlorhexidine 2 % prior Primer); G2(ASB); G3 (APP+CHX: Adper Prompt L-Pop + CHX); G4(APP); G5 (SBU+CHX: Single Bond Universal + CHX); and G6(SBU). Resin build-up was performed and teeth were subdivided regarding storage times (n=8): 72 h, 3 and 6 months. Next, SBS test was performed. At 72 hours, all equivalent groups (same adhesive system, different dentin treatment) showed no significant difference in SBS (P.05). Self-etch adhesive groups (with or without CHX) presented lower SBS...
Tipo: Journal article Palavras-chave: Shear bond strength; Dentin bond strength; Chlorhexidine; Adhesive systems; Adhesion.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300052
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Effect of photodynamic therapy on the extracellular matrix and associated components BJMBR
Pazos,M.d.C.; Nader,H.B..
In many countries, photodynamic therapy (PDT) has been recognized as a standard treatment for malignant conditions (for example, esophageal and lung cancers) and non-malignant ones such as age-related macular degeneration and actinic keratoses. The administration of a non-toxic photosensitizer, its selective retention in highly proliferating cells and the later activation of this molecule by light to form reactive oxygen species that cause cell death is the principle of PDT. Three important mechanisms are responsible for the PDT effectiveness: a) direct tumor cell kill; b) damage of the tumor vasculature; c) post-treatment immunological response associated with the leukocyte stimulation and release of many inflammatory mediators like cytokines, growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Photodynamic therapy; Extracellular matrix; Adhesion; Chemical mediators; Matrix metalloproteinases.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800002
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