Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 5
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG BJM
Assaf,Jean Claude; Atoui,Ali; Khoury,André El; Chokr,Ali; Louka,Nicolas.
ABSTRACT Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifugation. The mixture of L. rhamnosus GG and AFM1 was incubated for 18 h at 37 °C and the binding efficiency was determined by quantifying the unbound AFM1 using HPLC. The stability of the complexes viable bacteria-AFM1 and heat treated bacteria-AFM1 was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin M1; Lactic acid bacteria; L. rhamnosus GG; Milk.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100120
Imagem não selecionada

Imprime registro no formato completo
Aflatoxin M1 in milk by immunoaffinity column cleanup with TLC/HPLC determination BJM
Shundo,Luzia; Sabino,Myrna.
During 2002 and 2003, a total of 107 samples of raw, pasteurized and ultrahigh treated temperature (UHT) milk commercialized in the cities of São Paulo and Marília (SP) were analyzed for the presence of aflatoxin M1 (AFM1). AFM1 was detected in 79 (73.8%) of milk samples, ranging from <0.02 to 0.26 mug/L.The samples were analyzed using an immunoaffinity column for cleanup and a thin layer chromatography for determining AFM1. The parameters, such as recovery, repeatibility, detection and quantification limit were evaluated to optimize this method (in-house). Based on spiked samples, the recovery values ranged from 85.83 to 73.86% at levels of 0.010-0.50 mug/L, respectively, and the relative standard deviation for repeatibility ranged from 7.73 to 2.08%....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Aflatoxin M1; Thin layer chromatography; Immunoaffinity column.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200013
Imagem não selecionada

Imprime registro no formato completo
Aflatoxin M1 in samples of "minas" cheese commercialized in the city of Belo Horizonte - Minas Gerais/Brazil Ciênc. Tecnol. Aliment.
PRADO,Guilherme; OLIVEIRA,Marize Silva; PEREIRA,Maria Lúcia; ABRANTES,Fabiana Moreira; SANTOS,Luciana Gonçalves; VELOSO,Thais.
Milk products such as cheeses may be contaminated by aflatoxin M1 when dairy cattle have consumed feeds contaminated with aflatoxin B1. Samples of "Minas" cheeses (fresh, canastra and standard) were collected by the Inspection Service in the Mercado Central in Belo Horizonte city, Minas Gerais - Brazil. A purified extract was obtained by extraction with dichloromethane followed by a washing with n-hexane and immunoaffinity column purification. The quantification of aflatoxin M1 was done by high performance liquid chromatography (HPLC) using a fluorescence detector. Recoveries were about 75%. In 56 of the 75 samples (74.7%), the presence of aflatoxin M1 was detected in concentrations ranging between 0.02 and 6.92ng/g of cheese. In the positive cases ( >...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin M1; Cheese; HPLC.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612000000300020
Imagem não selecionada

Imprime registro no formato completo
Incidence of aflatoxin M1 contamination in milk, white cheese, kashar and butter from Sakarya, Turkey Ciênc. Tecnol. Aliment.
ÖZTÜRK YILMAZ,Suzan; ALTINCI,Alev.
Abstract The purpose of this research is to determine the incidence and occurrence of aflatoxin M1 (AFM1) in 104 samples of milk, white cheese, kashar and butter in Sakarya, Turkey. Followed by immunoaffinity column (IAC) purification, high performance liquid chromatography (HPLC)-fluorescence detection method was implemented to analyze the samples. Results show that 61.5% of milk, 40% of white cheese, 65.4% of kashar and 29.6% of butter contains AFM1. In 53 of samples (50.96%) are not determined AFM1. The amount of AFM1 in 51 samples (49.04%) ranges from 2.4 to 47.8 ng/L/kg. AFM1 levels do not exceed the limit set by Turkish regulations (50 ng/L/kg). However, the incidence of AFM1 contamination is a potential health risk for customers, particularly for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin M1; Milk; White cheese; Kashar; HPLC; Fluorescence detection.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000500190
Imagem não selecionada

Imprime registro no formato completo
Occurrence of aflatoxin M1 in parmesan cheese consumed in Minas Gerais, Brazil Ciência e Agrotecnologia
Prado,Guilherme; Oliveira,Marize Silva de; Lima,Adriana Souza; Moreira,Ana Paula Aprigio.
Aflatoxin M1 (AFM1) may occur in milk and milk products, resulting from the ingestion of aflatoxin B1 in feedstuffs by dairy cow. A total of 88 samples of Parmesan cheese marketed in Minas Gerais state, Brazil, from March 2004 to December 2004, were analyzed for AFM1 by high-performance liquid chromatography (HPLC) with a fluorescence detector following sample clean-up using immunoaffinity columns. AFM1 was detected in 40 of the 88 samples (46.4%). However, only two samples among these were contaminated at a level above the maximum permissible limit (250 ng kg-1) accepted by European Union for AFM1. No AFM1 maximum tolerance limit in cheese has been established in Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin M1; Cheese; Occurrence.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000600033
Registros recuperados: 5
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional