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Registros recuperados: 9
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Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment Electron. J. Biotechnol.
Kumar,Vinod; Sharma,Ashwani; Narasimha Prasad,Bellur Chayapathy; Bhaskar Gururaj,Harishchandra; Aswathanarayana Ravishankar,Gokare.
The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. Plant species differ in their temporal competence for transformation. But various physical and chemical methods are found to enhance transformation frequency. Agrobacterium rhizogenes mediated transformation efficiency was assessed under the influence of sonication, calcium treatment, acetosyringone and macerating enzymes in suitable combinations in Nicotiana tabacum as a model system. Manual wounding resulted in 21% transformation frequency. Where as sonication resulted in 2.2 fold increase, followed by sonication with CaCl2 treatment resulted in 2.5 fold increase and sonication with...
Tipo: Journal article Palavras-chave: Agrobacterium rhizogenes; Hairy roots; Nicotiana tabacum; Transformation frequency; Ultra-sonication.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400002
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Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina Biocell
Caro,Luis A.; Santecchia,Natalia; Marinangeli,Pablo A.; Curvetto,Néstor R.; Hernández,Luis F..
The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium rhizogenes; Algarrobo; Hairy roots; Indole-3-butyric acid; Nothofagus nervosa; Prosopis chilensis; Raulí; Rooting.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300002
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Changes in peroxidase and polypeptide profiles in Nicotiana tabacum L. transformed with Agrobacterium rhizogenes Ciência Rural
Echeverrigaray,Sergio.
Morphological and proteic modifications in Nicotiana tabacum L. transformed by Agrobacterium rhizogenes were evaluated by the comparison of normal and transformant plants regenerated from hairy-roots formed by the strains A4 or IB-642 of A. rhizogenes. Changes in apical dominance were observed in IB-642 transformants, which exhibited an abnormal development of axiliary buds. The electrophoretic analysis indicated an increase in peroxidase activity and the induction of several isozymes of this complex in the transformants. The SDS-PAGE patterns comparison allows to identify several changes, specially, the increase in 31-33 and 54 kD polypeptides in the transformants.. Biochemical analysis suggests the induction of a pathogen or stress like response of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Agrobacterium rhizogenes; Nicotiana tabacum; Electrophoretic analysis.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781995000200009
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Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare. Repositório Alice
HABIBI, P.; SA, M. F. G. de; SILVA, A. L. L. da; MAKHZOUM, A.; COSTA, J. da L.; BORGHETTI, I. A.; SOCCOL, C. R..
bitstream/item/148212/1/art-10.1007-s12298-016-0354-2.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Agrobacterium rhizogenes; Hairy roots; Calli; Regeneration; Origanum vulgare.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053999
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Ex vitro hairy root induction in detached peanut leaves for plant-nematode interaction studies. Repositório Alice
GUIMARAES, L. A.; PEREIRA, B. M.; ARAUJO, A. C. G.; GUIMARAES, P. M.; BRASILEIRO, A. C. M..
2017
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Expansin like-B; Meloidogyne; Arachis; Agrobacterium rhizogenes; Genetic transformation.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1070577
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Hairy root formation in alfalfa (Medicago sativa L.) by Agrobacterium rhizogenes OAK
HORIKAWA, Yoh; IIDA, Minoru; 堀川, 洋; 伊井田, 実.
http://www.obihiro.ac.jp/~library/kenkyu.html
Palavras-chave: Agrobacterium rhizogenes; Hairy root; Alfalfa (Medicago sativa L.)  ; Mikimopine.
Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3765
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Overexpression of wild Arachis lipocalin enhances root-knot nematode resistance in peanut hairy roots. Repositório Alice
PEREIRA, B. M.; GUIMARAES, L. A.; SOUZA, N. O. S.; SARAIVA, M. A. de P.; GUIMARAES, P. M.; BRASILEIRO, A. C. M..
bitstream/item/196683/1/Pereira2019-Article-OverexpressionOfWildArachisLip.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Agrobacterium rhizogenes; Arachis stenosperma; Temperature-induced lipocalin; Arachis Hypogaea; Meloidogyne Arenaria; Biotic stress.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108548
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Peroxidase production from hairy root cultures of red beet (Beta vulgaris) Electron. J. Biotechnol.
Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Kumar,Vinod; Lakshmanan,Venkatachalam; Venkataramareddy,Sreedhar Reddampalli; Aswathanarayana,Ravishankar Gokare.
The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-150, three each from A 2/83, A 20/83 and A4. All the clones showed true integration of T-DNA when tested by PCR and Southern hybridization methods. Each clone differed significantly from the others in growth, hormone dependency and POD production where LMG-150 produced highest biomass (140 g FW L-1) as well as POD (ranging from 8000-9000 U g-1 FW and 1.18 x 10(6) U L-1 with a specific activity of 600 U mg-1 protein) on hormone-free medium, both in shake-flask as...
Tipo: Journal article Palavras-chave: Agrobacterium rhizogenes; Auxins; Elicitation; T-DNA; Thermostability; Transformation.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200008
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Utilização de Agrobacterium rhizogenes para produção de culturas de raízes de milho. Repositório Alice
CARNEIRO, A. A.; GUIMARAES, A. N.; ALVES, M. de C.; CARNEIRO, N. P..
2014
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Agrobacterium rhizogenes; Raízes em cabeleira; Transformação genética.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/992684
Registros recuperados: 9
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