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Activities of selected digestive enzymes during larval development of large yellow croaker (Pseudosciaena crocea) ArchiMer
Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen.
Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,...
Tipo: Text Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf
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Alkaline phosphatase activity in plasma and liver of rats submitted to chronic exposure to fluoride BABT
Fernandes,Mileni da Silva; Iano,Flávia Godoy; Rocia,Vivian; Yanai,Marcela Mitsuko; Leite,Aline de Lima; Furlani,Tatiana Almeida; Buzalaf,Marília Afonso Rabelo; Oliveira,Rodrigo Cardoso de.
The aim of this study was to compare the effect of fluoride (F) on alkaline phosphatase activity in the liver and plasma of the rats. Four groups of male Wistar rats (n=6), which received drinking water containing 5, 15 or 50 ppm F or deionized water (control) throughout the experiment were included in the study. The animals were euthanized and had their tissues and blood plasma collected for the analysis of fluoride and alkaline phosphatase. There was an increase in F concentration in most tissues in the animals treated with higher F concentrations, except for the heart. The alkaline phosphatase assay showed an increase in the activity in the liver and blood plasma of the animals treated with fluoride concentrations of 15 and 50 ppm (p<0.05). This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline phosphatase; Fluoride; Liver; Kidney.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600014
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Altered Kidney Morphology and Enzymes in Streptozotocin Induced Diabetic Rats International Journal of Morphology
Zafar,Muhammad; Naeem-ul-Hassan Naqvi,Syed; Ahmed,Masood; Kaimkhani,Zahid Ali.
We studied the effects of streptozotocin (STZ)-induced diabetes on kidney morphology, anatomy, architecture and on the activities of aminotransferases (AST and ALT), alkaline phosphatase (ALP) and pseudocholinesterase (PChE) in albino rats. The aim of the study was to investigate the association between diabetic kidney complications and kidney enzyme alterations. This study was performed in the Department of Anatomy and Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi and Pathology department of College of Physicians &amp; Surgeons (CPSP) Pakistan in 2007-08. Diabetes was induced by a single dose of STZ (45 mg/kg, b.w.) given intraperitoneally in sodium citrate buffer at pH 4.5. Eighty (80) albino rats were divided into five...
Tipo: Journal article Palavras-chave: Streptozotocin; Aminotransferases; Alkaline phosphatase; Pseudocholinesterase; STZ-diabetes; Rats.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300024
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Altered Liver Morphology and Enzymes in Streptozotocin Induced Diabetic Rats International Journal of Morphology
Zafar,Muhammad; Naeem-ul-Hassan Naqvi,Syed; Ahmed,Masood; Kaimkhani,Zahid Ali.
This study was undertaken to evaluate the relationship and effects of diabetes on liver morphology, architecture and function. The hepatic effects of diabetes were evaluated in vivo using streptozotocin (STZ)-induced diabetic rats as an experimental model. The degree of hepatic dysfunction was measured by using biochemical parameters like serum transaminases (ALT and AST), alkaline phosphatase (ALP)and pseudocholinesterase (PChE) while the histopathological studies were carried out to support the enzymic Parameters. The aim of the study was to investigate the association between diabetic hepatic complications and liver enzyme alterations. This study was performed in the Department of Anatomy; Institute of Pharmaceutical Sciences and Institute of...
Tipo: Journal article Palavras-chave: Streptozotocin; Aminotransferases; Alkaline phosphatase; Pseudocholinesterase; STZ-diabetes; Hepatic injury.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300015
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Application of Scharer's quantitative method for the determination of residual alkaline phosphatase activity in standard Minas Arq. Bras. Med. Vet. Zootec.
Soares,C.F.; Fonseca,L.M.; Leite,M.O.; Oliveira,M.C.P.P..
Milk pasteurization is a critical issue in the dairy industry, and failures in this process can affect final product safety. Scharer's enzymatic method is still traditionally used to verify pasteurization efficiency compliance, and it is based on screening for residual alkaline phosphatase in milk. Although several methods are used to quantify enzymatic activity to assess milk pasteurization efficiency, there is a small amount of published data regarding the use of these methods to quantify alkaline phosphatase in cheese. In this study, the Scharer's modified method was used to determine the levels of residual alkaline phosphatase in standard minas cheese, before and after 20 days of ripening. The cheeses were made using raw or pasteurized milk with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline phosphatase; Standard Minas cheese; Scharer's modified method; Spectrophotometry.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400039
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Atividade microbiana em solo do Semi-Árido sob cultivo de Atriplex nummularia. Repositório Alice
PEREIRA, S.V.; MARTINEZ, C.R.; PORTO, E.R.; OLIVEIRA, B.R.B.; MAIA, L.C..
Métodos para avaliar a atividade microbiana no solo são fundamentais no monitoramento ambiental de áreas degradadas. O objetivo deste trabalho foi investigar a atividade microbiana de solo do semi-árido cultivado com Atriplex nummularia Lindl. em áreas que receberam rejeito salino durante um e três anos, em comparação com um solo nativo, sem cultivo e não irrigada. O solo cultivado por três anos e que recebeu rejeito salino apresentou, no período seco, valores de pH, CE e atividade de hidrólise do diacetato de fluoresceína (FDA) superiores aos das demais áreas. No entanto, foi observada correlação negativa entre o carbono microbiano e os valores do quociente metabólico (qCO2). A biomassa microbiana e a fosfatase alcalina também foram superiores no solo...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Emissão de CO2; Biomassa microbiana; Diacetato de fluoresceína; Fosfatase alcalina; CO2 emission; Microbial biomass; Fluorescein diacetate; Alkaline phosphatase.
Ano: 2004 URL: http://www.alice.cnptia.embrapa.br/handle/doc/112509
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Biochemical and Mitochondrial Changes Induced by Cd, Fe and Zn in Limnodrillus hoffmeisteri International Journal of Morphology
Grajeda y Ortega,María de los Angeles; Ortiz Ordoñez,Esperanza; Favari,Liliana; Shibayama,Mineko; Silva Olivares,Angélica; López López,Eugenia.
The effects of sublethal concentrations of cadmium (0.64 µg/L), iron (0.043 mg/L) and zinc (0.31 mg/L) and a mixture of these metals on succinate dehydrogenase (SD) and alkaline phosphatase (AP) activity and on structural changes in the mitochondria of epithelium cells of the digestive tract were examined in the oligochaete Limnodrillus hoffmeisteri after 96 h of exposure in artificial sediments. SD activity was significantly inhibited, particularly in treatments with Cd alone (92.57%), while AP increased its activity with Cd alone (73.23%). However, when this metal was mixed with Fe and Zn, the inhibition of SD activity was lower (67.82%) than with Cd alone, showing an antagonistic effect and AP increased its activity (73.26%). Mitochondria were...
Tipo: Journal article Palavras-chave: Heavy metals; Mixture; Alkaline phosphatase; Succinate dehydrogenase activity; Limnodrillus hoffmeisteri; Epithelium cells; Mitochondria.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200018
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Breeder age and bone development in broiler chicken embryos Arq. Bras. Med. Vet. Zootec.
Alfonso-Torres,K.A.; Gargaglioni,L.H.; Pizauro,J.M.; Faria Filho,D.E.; Furlan,R.L.; Macari,M..
The effect of breeder age on long bone development was studied in chicken embryos from 12 days of incubation until hatching. Fertile eggs were incubated and randomly assigned in a 2 x 6 factorial arrangement (two breeder ages - 38 and 60 weeks and six incubation days - 12, 14, 16, 18, 20, and 21). Enzymatic activity of acid and alkaline phosphatases in tibial epiphyses and weights as well as length and width in tibias and femurs of the embryos were determined. Tartrate-resistant acid and alkaline phosphatases activity in epiphyses was not affected by breeder age. Absolute weight and width of femur and tibia were larger in 60-week-old embryos compared to 38-week-old. Enzymatic activity and morphometric measurements increased with incubation day,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Chicken embryo; Alkaline phosphatase; Epiphyses; Growth plate; Long bones.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100031
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Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi ArchiMer
Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques.
This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively....
Tipo: Text Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf
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Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity BJMBR
Mota,A.; Silva,P.; Neves,D.; Lemos,C.; Calhau,C.; Torres,D.; Martel,F.; Fraga,H.; Ribeiro,L.; Alçada,M.N.M.P.; Pinho,M.J.; Negrão,M.R.; Pedrosa,R.; Guerreiro,S.; Guimarães,J.T.; Azevedo,I.; Martins,M.J..
Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol p-nitrophenol·mg protein-1·min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heart; Alkaline phosphatase; Polyphenol-rich beverages; Steroid hormones; Methylxanthines.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700009
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Contribution of matrix vesicles and alkaline phosphatase to ectopic bone formation BJMBR
Ciancaglini,P.; Simão,A.M.S.; Camolezi,F.L.; Millán,J.L.; Pizauro,J.M..
Endochondral calcification involves the participation of matrix vesicles (MVs), but it remains unclear whether calcification ectopically induced by implants of demineralized bone matrix also proceeds via MVs. Ectopic bone formation was induced by implanting rat demineralized diaphyseal bone matrix into the dorsal subcutaneous tissue of Wistar rats and was examined histologically and biochemically. Budding of MVs from chondrocytes was observed to serve as nucleation sites for mineralization during induced ectopic osteogenesis, presenting a diameter with Gaussian distribution with a median of 306 ± 103 nm. While the role of tissue-nonspecific alkaline phosphatase (TNAP) during mineralization involves hydrolysis of inorganic pyrophosphate (PPi), it is unclear...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Matrix vesicles; Endochondral ossification; Osseous plate; Alkaline phosphatase; Ectopic mineralization; Calcification.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500006
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Dissolving mechanism of strain P17 on insoluble phosphorus of yellow-brown soil BJM
Chuan-qing,Zhong; Guang-xiang,Cao; Wei-yi,Huang; Xing-she,Luan; Yi-fei,Yang.
Strain P17 was a bacterial strain identified as Bacillus megaterium isolated from ground accumulating phosphate rock powder. The fermentation broth of strain P17 and the yellow-brown soil from Nanjing Agricultural University garden were collected to conduct this study. The simulation of fixed insoluble phosphorous forms after applying calcium superphosphate into yellow-brown soil was performed in pots, while available P and total P of soil were extremely positive correlative with those of groundwater. Then the dissolving effect of strain P17 on insoluble P of yellow-brown soil was studied. Results showed that Bacillus megaterium strain P17 had notable solubilizing effect on insoluble phosphates formed when too much water-soluble phosphorous fertilizer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium superphosphate; P-solubilizing microbes; Acid phosphatase; Alkaline phosphatase.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300025
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Do distinct water chemistry, reservoir age and disturbance make any difference on phosphatase activity? IPB - Escola Superior Agrária
Geraldes, Ana Maria; Boavida, Maria José.
Alkaline phosphatase activity was assessed concomitantly with total phosphorus, orthophosphate and phosphomonoester concentrations in two meso-eutrophic reservoirs with distinct age and subjected to different kinds of environmental influence. Differences in conductivity, temperature and pH were found. However, during the study period alkaline phosphatase activity was similar in both reservoirs. Water colour was higher in S. Serrada Reservoir. This fact can be related to (1) reservoir age (2) high internal disturbance (3) large imputs of allochthonous detritus, resulting from the combined effect of grazing, fire and catchment slope. Despite the high water colour recorded in S. Serrada, alkaline phosphatase activity was apparently not inactivated by humic...
Tipo: Article Palavras-chave: Alkaline phosphatase; Orthophosphate regeneration; Phytoplankton; Zooplankton; Reservoirs.
Ano: 2003 URL: http://hdl.handle.net/10198/705
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Enzyme activities of phytoplankton in the South Shetland Islands (Antarctica) in relation to nutrients and primary production RChHN
IRIARTE,JOSÉ L; GONZÁLEZ,RODRIGO R; QUIÑONES,RENATO A; KANG,SUNG-HO; SHIM,JAE H; VALENZUELA,CYNTHIA P.
Given the potential significance of enzyme activities as a link between internal metabolic pathways and environmental nutrients, we investigated the relationships of nitrate reductase (NR) and alkaline phosphatase (AP) with primary production and inorganic nutrients in South Shetland Islands, Antarctica. Enzymatic activities of the phytoplankton (0.7-210 µm), primary productivity, autotrophic biomass and inorganic nutrients were studied in the upper 100 m depth at nine stations during a cruise in the northwestern area of South Shetland Islands (Antarctica), during late austral spring (December 2000). NR activities fluctuated between 0 and 42.8 nmol L-1 h-1 (mean = 10.08 nmol L-1 h-1, SD = 10.42 nmol L-1 h-1), AP activities between 0.81 and 5.67 nmol L-1...
Tipo: Journal article Palavras-chave: Nitrate reductase; Alkaline phosphatase; Primary productivity; Chlorophyll; Antarctica.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2006000400009
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Enzyme activity and microbial biomass in an Oxisol amended with sewage sludge contaminated with nickel Scientia Agricola
Revoredo,Marcos Donizeti; Melo,Wanderley José de.
The role of nickel in soils of tropical areas under the application of sewage sludge is still not very well known. This study was carried out under greenhouse conditions in Jaboticabal, São Paulo State, Brazil, with the objective of evaluating the impact of the application of sewage sludge previously contaminated with rates of nickel (329, 502, 746 and 1119 mg kg-1, dry basis) on a soil cropped with sorghum, in relation to soil enzyme activity and soil microbial biomass. Soil samples were collected at the beginning and the end of the experiment. The experimental design was completely randomized, with five treatments (control and four rates of Ni in the sewage sludge) and four replications. C and N of the soil microbial biomass and enzyme activities (acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heavy metal; Biosolid; Acid phosphatase; Alkaline phosphatase; Sorghum.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000100009
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Fosfatase ácida e alcalina em solo sob manejo do sistema integração lavoura pecuária e floresta. Repositório Alice
MOREIRA, E. D. S.; MARRIEL, I. E.; SANTOS, C. A. dos; SOUSA, J. C. da C. de; GONTIJO NETO, M. M.; LANA, A. M. Q.; PAIVA, C. A. de O..
2016
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Sistemas integrados; Enzima; Fósforo; Acid phosphatase; Alkaline phosphatase; Urochloa brizantha.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1054902
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Growth and phosphorus uptake by the toxic dinoflagellate Alexandrium catenella (dinophyceae) in response to phosphate limitation ArchiMer
Jauzein, Cecile; Labry, Claire; Youenou, Agnes; Quere, Julien; Delmas, Daniel; Collos, Yves.
Alexandrium catenella (Whedon et Kof.) Balech has exhibited seasonal recurrent blooms in the Thau lagoon (South of France) since first reported in 1995. Its appearance followed a strong decrease (90%) in phosphate (PO(4)3-) concentrations in this environment over the 1970-1995 period. To determine if this dinoflagellate species has a competitive advantage in PO(4)3--limited conditions in terms of nutrient acquisition, semicontinuous cultures were carried out to characterize phosphorus (P) uptake by A. catenella cells along a P-limitation gradient using different dilution rates (DRs). Use of both inorganic and organic P was investigated from measurements of 33PO(4)3- uptake and alkaline phosphatase activity (APA), respectively. P status was estimated from...
Tipo: Text Palavras-chave: Alexandrium catenella; Alkaline phosphatase; Limitation; Phosphorus; Semicontinuous cultures.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12641/9570.pdf
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Identification of midgut membrane proteins from different instars of Helicoverpa armigera that bind to Cry1Ac toxin. Repositório Alice
SILVA, I. H. S.; GOMEZ, I.; SANCHEZ, J.; CASTRO, D. L. M.; VALICENTE, F. H.; SOBERON, M.; POLANCZYK, R. A.; BRAVO, A..
bitstream/item/195733/1/Identification-midgut.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Pull down; Bacillus Thuringiensis; Controle Biológico; Alkaline phosphatase.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108142
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In Vivo Detoxification of Ochratoxin A by Highly Porous Magnetic Nanocomposites Prepared from Coconut Shell Rev. Bras. Ciênc. Avic.
Gulfam,N; Zahoor,M; Khisroom,M; Khan,FA.
ABSTRACT The current study was conducted to elucidate the clinical effect of ochratoxin A in Gallus gallusdomesticus and to ameliorate its toxic effects by the development and characterization of highly porous carbon-based adsorbent derived from coconut shell. A series of experiments were performed on one day-old chicks (Group A to F). Clinical signs of the positive control (group B fed on 400 ppb ochratoxin A contaminated feed) included depression, diarrhea, increased water intake, low body weight, high degree of genotoxicity, swollen and hemorrhagic kidneys and liver. Serum alanine amino transferase (ALT), alkaline phosphatase (ALP), creatinine and urea concentration significantly increased while albumin, globulin and total proteins were found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Serum alanine transferase; Alkaline phosphatase; Clinical signs; Gross lesions.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400675
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Influence of partial substitution of dietary fish meal on the activity of digestive enzymes in the intestinal brush border membrane of gilthead sea bream, Sparus aurata and goldfish, Carassius auratus ArchiMer
Silva, Flavia; Nicoli, Jacques R.; Zambonino, Jose-luis; Le Gall, Marie-madeleine; Kaushik, Sadasivam; Gatesoupe, François-joel.
The sustainable composition of diets of high nutritional value is of the utmost importance for intensive aquaculture. Digestion and absorption of nutrients depends on the activity of the digestive enzymes, in particular those located in the brush border membrane of enterocytes, which are responsible for the final stages of breaking down and absorption of nutrients. In the present study, the substitution of fish meal by lupin or rapeseed meal in the diet was evaluated on gilthead sea bream (Sparus aurata) and goldfish (Carassius auratus). The objectives were to compare the activities of intestinal brush border enzymes in both species fed the control and experimental diets. When gilthead sea bream were fed the vegetable diets, significantly lower activities...
Tipo: Text Palavras-chave: Sparus aurata; Carassius auratus; Fish meal replacement; Brush border membrane; Digestive enzymes; Alkaline phosphatase; Aminopeptidase N; Maltase; Glutamyl transpeptidase.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00009/11975/8679.pdf
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