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Action of gibberellic acid (GA3) on dormancy and activity of alpha-amylase in rice seeds Rev. bras. sementes
Vieira,Antônio Rodrigues; Vieira,Maria das Graças Guimarães Carvalho; Fraga,Antônio C.; Oliveira,João Almir; Santos,Custódio D. dos.
To evaluate the effectiveness of gibberellic acid (GA3) in breaking rice seed dormancy and the use of alpha-amylase enzyme activity as an indicator of the dormancy level, seed from the intensively dormant irrigated cultivar Urucuia were used. The seeds were submitted to a pre-drying process in a forced air circulation chamber under 40ºC during 7 days and submersed in 30 mL of GA3 solution under 0, 10, 30 and 60 mg/L H2O concentrations, during 2, 24 and 36 hours. After the treatments, the alpha-amylase activity was determined by using the polyacrilamide electrophoresis and spectrophotometry. At the same time, the germination test was made. The results indicated a gain in germination and in alpha-amylase activity in higher concentrations and soaking time of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rice seeds; Dormancy; Germination; Gibberellic acid (GA3); Alpha-amylase.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000200008
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Culture conditions for the production of thermostable amylase by Bacillus sp BJM
Teodoro,Carlos Eduardo de Souza; Martins,Meire Lelis Leal.
Studies on the alpha-amylase production were carried out with a bacterial strain isolated from a soil sample. The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the mineral medium shortened the lag period and improved the growth and alpha-amylase synthesis. The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase, demonstrating that a classical glucose effect is operative in this organism. The optimum temperature and initial medium pH for amylase synthesis by the organism were 50°C and 7.0 respectively. The optimal pH and temperature for activity were 6.0 and 50°C respectively. The enzyme extract retained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha-amylase; Thermophilic bacterium; Thermostable enzymes; Bacillus sp.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400011
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Malting quality and genetic diversity in brazilian elite barley germplasm. Repositório Alice
TONIAZZO, C.; BRAMMER, S. P.; MINELLA, E.; CAVERZAN, A.; WIETHÖLTER, P.; CASASSOLA, A.; GRANDO, M. F..
bitstream/item/165546/1/ID44155-2017v9n6IntJCurrRes.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Kolbach index; Diastatic power; Hordeum vulgare; Cevada; Malte; Variação genética; Hordeum vulgare; Barley; Beta-glucans; Alpha-amylase; Microsatellite repeats; Malting quality; Genetic variation.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078136
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Modification of the activity of an alpha-amylase from Bacillus licheniformis by several surfactants Electron. J. Biotechnol.
Bravo Rodríguez,Vicente; Jurado Alameda,Encarnación; Martínez Gallegos,Juan Francisco; Reyes Requena,Antonia; García López,Ana Isabel; Sampaio Cabral,Joaquim Manuel; Fernandes,Pedro; Pina da Fonseca,Luis Joaquim.
The influence of different commercial surfactants on the enzymatic activity of a commercial alpha-amylase from Bacillus licheniformis (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant...
Tipo: Journal article Palavras-chave: Alpha-amylase; Alkyl polyglycosides; Enzymatic activity; Fatty alcohol ethoxylates; Linear alkyl benzene sulfonate; Nonyl phenol ethoxylate.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500012
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Rendimento e processo germinativo do grão na espiga de genótipos de trigo. Repositório Alice
FELICIO, J. C.; CAMARGO, C.E. de O.; GERMANI, R.; FREITAS, J.G. de.
O objetivo deste trabalho foi estudar o processo germinativo dos grãos na espiga, o rendimento de grãos, a adaptabilidade e a estabilidade em genótipos de trigo (Triticum aestivum L.) sensíveis às variações ambientais. Os experimentos foram instalados no Núcleo Experimental do Instituto Agronômico, em Campinas, SP, no período de 1996 a 1998. A germinação na espiga foi avaliada pelo método do "Falling Number", que consiste em determinar o nível de atividade da alfa-amilase nos grãos. A segunda e a terceira colheitas foram realizadas ao sete e quatorze dias após a primeira colheita. O atraso da colheita determinou redução no rendimento, notadamente na terceira colheita; os teores de alfa-amilase foram maiores também na terceira colheita, em virtude da...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triticum aestivum; Germinabilidade; Alfa-amilase; Época de semeadura; Germinability; Alpha-amylase; Sowing date.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/106780
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Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas ArchiMer
Huvet, Arnaud; Jeffroy, Fanny; Daniel, Jean-yves; Quere, Claudie; Le Souchu, Pierrick; Van Wormhoudt, A.; Boudry, Pierre; Moal, Jeanne; Samain, Jean-francois.
In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values...
Tipo: Text Palavras-chave: Alpha-amylase; Crassostrea gigas; Digestive enzyme; Michaelis-Menten constant; Regulation.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00087/19869/17521.pdf
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The Bread making qualities of bread dough supplemented with whole wheat flour and treated with enzymes OAK
Matsushita, Koki; Santiago, Dennis Marvin; Noda, Tatsuya; Tsuboi, Kazumasa; Kawakami, Sakura; Yamauchi, Hiroaki.
Whole wheat flour (WWF) contains various kinds of functional ingredients, such as dietary fiber, resistant starch, minerals, etc., and bread containing WWF is in great demand in many countries because of its health benefits. Although dough with WWF often results in lower quality bread, previous studies suggested that this could be improved by the addition of suitable enzymes. This study investigated the effects of WWF substitution and enzyme treatments using alpha-amylase (AM) and hemicellulase (HC) on bread making qualities. Results showed that the addition of WWF produced dough with low gas retention of dough (GRD) and specific loaf volume (SLV). However, AM and HC drastically improved both GRD and SLV of WWF-substituted dough and bread by degrading...
Palavras-chave: Whole wheat flour; Bread making quality; Alpha-amylase; Hemicellulase; Enzyme.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4486
Registros recuperados: 7
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