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A method for the determination of two Alternaria toxins, alternariol and alternariol monomethyl ether, in tomato products BJM
Motta,Silvana da; Soares,Lucia M. Valente.
Tomatoes, as any soft skinned fruit, are easily susceptible to fungal rot. Alternaria is the genus most frequently encountered in tomatoes. A. alternata and other spp. have been shown to produce the toxins alternariol monomethyl ether (AME) and alternariol (AOH) in tomatoes. A method for determining AME and AOH in tomato products was developed and evaluated. The method involves extraction with methanol, clarification with ammonium sulfate, and partition to chloroform. Quantification was conducted by high performance liquid chromatography with diode array detector (DAD). Average recoveries were 98.7% and 84.1% for AME and AOH, respectively. The quantification limits of the method, defined as the minimum amount that allowed quantification and confirmation by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternaria; Mycotoxins; Alternariol monomethyl ether; Alternariol; Tomatoes.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400015
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Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on wheat BJM
Oviedo,María Silvina; Sturm,María Elena; Reynoso,María Marta; Chulze,Sofia Noemi; Ramirez,María Laura.
The objectives of this study were to evaluate the ability to produce alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) by A. alternata and A. infectoria strains recovered from wheat kernels obtained from one of the main production area in Argentina; to confirm using AFLPs molecular markers the identify of the isolates up to species level, and to evaluate the intra and inter-specific genetic diversity of these two Alternaria species. Among all the Alternaria strains tested (254), 84% of them were able to produce mycotoxins. The most frequent profile of toxin production found was the co-production of AOH and AME in both species tested. TA was only produced by strains of A. alternata. Amplified fragment polymorphism (AFLPs)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternaria alternata; Alternaria infectoria; Alternariol; Alternariol monomethyl ether; Tenuazonic acid; AFLPs; Wheat; Genetic variation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200017
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