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Characterization of organic matter in spodosol amazonian by fluorescence spectroscopy. Repositório Alice
TADINI, A. M.; HAJJOUL, H.; NICOLODELLI, G.; MOUNIER, S.; MONTES, C. R.; MILORI, D. M. B. P..
bitstream/item/165808/1/PROCI-17-Characterization-of-organic-matter....pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Amazonian soil; Characterization; Fluorescence; Humic acid; Lifetime.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078464
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Protease with collagenolytic activity produced by Bacillus sp. DPUA 1728 from Amazonian soil BJM
Lima,Lorena A.; Cruz Filho,Raimundo F.; Santos,Januário G. dos; Silva,Wilson C..
Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases with collagenolytic activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design with four repetitions at the center point was developed to analyze the effects and interactions between initial pH, temperature and the concentration of gelatin. Based on the results of the first 23 full factorial design, a successive 23 full factorial design was performed. The most favorable production conditions were found to be 1.5% (w/v) gelatin, pH 9.0 and 37 °C with enzymatic activity of 86.27 U/mL. The enzyme showed optimal activity at 50 °C and pH 9.0, and it was stable over wide pH (7.2-10.0) and temperature (45 °C-60 °C)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collagenolytic protease; Bacillus sp; Factorial design; Amazonian soil.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401217
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