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Activities of selected digestive enzymes during larval development of large yellow croaker (Pseudosciaena crocea) ArchiMer
Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen.
Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,...
Tipo: Text Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf
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Adaptation des enzymes digestives à l'alimentation chez la crevette Penaeus vannamei (Crustacea Decapoda) ArchiMer
Le Moullac, Gilles.
L'adaptation des enzymes digestives chez la crevette P. vannamei a été étudiée en fonction des cycles biologiques en relation avec les constituants de l'alimentation principalement les glucides (amidon) et les protéines. La trypsins, la chymotrypsine et 1'amylase, 3 enzymes majeures, chez P. vannamei ont été purifiées et caractérisées. La trypsine est formée de 3 isoenzymes dont le poids moléculaire apparent est compris entre 31 et 3 2 kDa. La nature glycosylée de la trypsine est révélée. La chymotrypsine est formée de 2 isoenzymes de poids moléculaire similaire (24 kDa). L'amylase purifiée est constituée de 2 isoenzymes dont le poids moléculaire apparent est de 31 kDa. Les propriétés biochimiques sont étudiées. L'étude immunologique a permis de mesurer...
Tipo: Text Palavras-chave: Penaeus vannamei; Hécatopancréas; Biochimie; Immunologie; Enzymes digestives; Trypsine; Chymotrypsine; Amylase; Larves; Adultes; Intermue; Protéines alimentaires; Amidon; Nutrition; Adaptation.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00029/14012/11198.pdf
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Adaptation of digestive enzymes to dietary protein, carbohydrate and fibre levels and influence of protein and carbohydrate quality in Penaeus vannamei larvae (Crustacea, Decapoda) ArchiMer
Le Moullac, Gilles; Van Wormhoudt, A; Aquacop, Aquacop.
Activity of digestive enzymes (trypsin, chymotrypsin and amylase) was studied in relation with food in Penaeus vannamei larvae first stages. Microparticles containing casein involved the decrease of soluble protein content and enzyme activities. Specific trypsin activity but not chymotrypsin was correlated (p<1%) to casein level between 10 and 60% in microparticles. Cellulose fibres in food seemed to have little effect on digestive enzymes. Starch, between 1 and 20% in microparticles, had no influence on specific amylase activity. The effect of different feeding conditions were also tested on growth. No clear relationship between growth and enzyme is established since only trypsin is concerned in the case of a casein dose effect in the food. The protein...
Tipo: Text Palavras-chave: Amylase; Chymotrypsin; Trypsin; Digestive enzymes; Food; Larvae; Penaeus vannamei; Amylase; Chymotrypsine; Trypsine; Enzymes digestives; Alimentation; Larve; Penaeus vannamei.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-4431.pdf
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Amylase production by endophytic fungi Cylindrocephalum sp. isolated from medicinal plant Alpinia calcarata (Haw.) Roscoe BJM
Sunitha.,V. H.; Ramesha.,A.; Savitha.,J.; Srinivas.,C.
Amylases are among the most important enzymes used in modern biotechnology particularly in the process involving starch hydrolysis. Fungal amylase has large applications in food and pharmaceutical industries. Considering these facts, endophytic fungi isolated from the plant Alpinia calcarata (Haw.) Roscoe were screened for amylolytic activity on glucose yeast extract peptone agar (GYP) medium. Among thirty isolates of endophytic fungi, isolate number seven identified as Cylindrocephalum sp. (Ac-7) showed highest amylolytic activity and was taken for further study. Influence of various physical and chemical factors such as pH, temperature, carbon and nitrogen sources on amylase production in liquid media were studied. The maximal amylase production was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpinia calcarata Roscoe; Endophytic fungi; Cylindrocephalum sp.; Amylase.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300049
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Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp. BJM
Saxena,Rajshree; Singh,Rajni.
Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid State Fermentation; Agro-industrial wastes; Amylase; Enzyme optimization; Stability; Bacillus sp.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014
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Bioprospection and characterization of the amylolytic activity by filamentous fungi from Brazilian Atlantic Forest Biota Neotropica
Almeida,Paula Zaghetto de; Pereira,Marita Gimenez; Carvalho,Caio Cesar de; Heinen,Paulo Ricardo; Ziotti,Luciana Sobrani; Messias,Josana Maria; Jorge,João Atilio; Polizeli,Maria de Lourdes Teixeira de Moraes.
Abstract Filamentous fungi are widely diverse and ubiquitous organisms. Such biodiversity is barely known, making room for a great potential still to be discovered, especially in tropical environments - which are favorable to growth and species variety. Filamentous fungi are extensively applied to the production of industrial enzymes, such as the amylases. This class of enzymes acts in the hydrolysis of starch to glucose or maltooligosaccharides. In this work twenty-five filamentous fungi were isolated from samples of decomposing material collected in the Brazilian Atlantic Forest. The two best amylase producers were identified as Aspergillus brasiliensis and Rhizopus oryzae. Both are mesophilic, they grow well in organic nitrogen-rich media produce great...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Filamentous fungi; Aspergillus brasiliensis; Rhizopus oryzae; Glucoamylase; Bioprospection.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032017000300204
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Caracterização de clones de mandioca com e sem casca a serem utilizados na alimentação animal. Repositório Alice
SANTOS, L. F. dos; SOUZA, V. dos S.; OLIVEIRA, L. A. de; JESUS, J. L. de; SANTOS, V. da S.; BRITO, J. Á. G. de.
A mandioca (Manihot esculenta Crantz) é uma fonte de carboidrato, que é o componente quantitativamente mais importante das rações para diferentes espécies de animais. Assim, o presente trabalho teve como objetivo avaliar o teor de compostos ianogênicos, matéria seca, sólidos solúveis e relação amilose/amilopectina de três clones a serem utilizados na produção de farinha de raspa para alimentação animal.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sólidos solúveis; Mandioca; Nutrção animal; Composto cianogênico; Amilose; Matéria seca; Cassava; Amylase; Phytotoxicity; Dry matter content; Organic wastes.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1011132
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Caracterização do amido de acessos de mandioca. Repositório Alice
SANTANA, H. M.; FIUZA, A. U. R.; SILVA, J. F. M. da; SILVA, L. do A. O. da; SILVA, M. C. S. de M.; OLIVEIRA, L. A. de; JESUS, J. L. de; OLIVEIRA, E. J. de.
A mandioca (Manihot esculenta Crantz) desempenha elevada importância social para os países em desenvolvimento devido sua ampla utilização, seja como alimento ou como matéria prima, para a extração de amido
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mandioca; Amido; Amilose; Cassava; Starch; Manihot esculenta; Acidity; Amylase.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1011147
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Changes in amylase activity starch and sugars contents in mango fruits pulp cv. Tommy Atkins with spongy tissue BABT
Lima,Luiz Carlos de Oliveira; Chitarra,Adimilson Bosco; Chitarra,Maria Isabel F..
Changes in amylase activity, starch and reducing and non-reducing sugars contents were monitored during ripening of mango fruits (Mangifera indica L.). The climateric raising in mango fruit is marked by an appreciable increase in the activity of amylase, reducing and non-reducing sugars contents and decrease in the starch content. The fruit affected with spongy tissue exhibited much lower amylase activity and reducing and non-reducing sugars, but exhibited much higher starch content during storage at 12 ± 2° C and 90 ± 5% RH for 28 days, when compared to healthy tissue of ‘Tommy Atkins’. Whether this is caused due to adverse effects on certain enzyme activities during ripening is not clearly known. These dates showed that carbohydrate metabolism is an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Starch; Amylase; Reducing sugars; Non-reducing sugars.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100008
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Citral as food additive for common snook - zootechnical parameters and digestive enzymes Ciência Rural
Michelotti,Bruna Tomazetti; Mori,Natacha Cossettin; Magnotti,Caio Cesar Franca; Heinzmann,Berta Maria; Almeida,Ana Paula Gottlieb; Cerqueira,Vinicius Ronzani; Baldisserotto,Bernardo.
ABSTRACT: Essential oils of plants whose main compound is citral showed beneficial effects when added to fish feed. The objective of the present study was to evaluate the dietary effect of the addition of citral on zootechnical parameters and digestive enzyme activities of Centropomus undecimalis. Juveniles were fed for 45 days with diets containing different amounts of citral (0.0 - control, 0.5, 1.0, and 2.0 mL per kg of diet). The water quality parameters were kept stable during the experiment and no mortality was observed. At the end of the experimental period, the treatment 0.5 mL citral per kg of diet had the lowest weight gain and specific growth rate, and the highest feed conversion, while the same parameters did not differ between the other...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Centropomus undecimalis; Amylase; Pepsin; Lipase; Growth; Marine fish farming.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400651
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Comparative study on production of α-Amylase from Bacillus licheniformis strains BJM
Divakaran,Dibu; Chandran,Aswathi; Pratap Chandran,R.
Alpha amylase (α-1, 4-glucan-glucanhydrolase, EC 3.2.1.1), an extracellular enzyme, degrades α, 1-4 glucosidic linkages of starch and related substrates in an endo-fashion producing oligosaccharides including maltose, glucose and alpha limit dextrin (7). The present study deals with the production and comparative study of production of α-amylase from two strains of Bacillus licheniformis, MTCC 2617 and 2618, by using four different substrates, starch, rice, wheat and ragi powder as carbon source by submerged fermentation. The effect of varying pH and incubation temperature, activator, inhibitor, and substrate concentration was investigated on the activity of α-amylase produced by MTCC strain 2618. The results shows that the production of the α-amylase by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Bacillus licheniformis; Enzyme; Extracellular; Glucose.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400022
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Cooking quality of upland and lowland rice characterized by different methods Ciênc. Tecnol. Aliment.
Garcia,Diva Mendonça; Bassinello,Priscila Zaczuk; Ascheri,Diego Ramiro Palmirez; Ascheri,José Luis Ramirez; Trovo,José Benedito; Cobucci,Rosário de Maria Arouche.
Rice cooking quality is usually evaluated by texture and stickiness characteristics using many different methods. Gelatinization temperature, amylose content, viscosity (Brookfield viscometer and Rapid Visco Analyzer), and sensory analysis were performed to characterize culinary quality of rice grains produced under two cropping systems and submitted to different technologies. All samples from the upland cropping system and two from the irrigated cropping system presented intermediate amylose content. Regarding stickiness, BRS Primavera, BRS Sertaneja, and BRS Tropical showed loose cooked grains. Irrigated cultivars presented less viscosity and were softer than upland cultivars. Upland grain samples had similar profile on the viscoamylografic curve, but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa L; Texture; Stickiness; Amylase; Gelatinization temperature; Cluster.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200010
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Corn and soybean meal metabolizable energy with the addition of exogenous enzymes for poultry Rev. Bras. Ciênc. Avic.
Dourado,LRB; Sakomura,NK; Barbosa,NAA; Bonato,MA; Kawuauchi,IM; Fernandes,JBK; Costa,FGP.
Two metabolism assays were carried out to determine corn and soybean meal metabolizable energy when enzymes were added. In the first trial, 35 cockerels per studied feedstuff (corn and soybean meal) were distributed in a completely randomized experimental design with four treatments of seven replicates of one bird each. The evaluated treatments were: ingredient (corn and soybean meal) with no enzyme addition, with the addition of an enzyme complex (xylanase, amylase, protease - XAP), xylanase, or phytase. Precise feeding method was used to determine true metabolizable energy corrected for nitrogen balance (TMEn). The use of enzymes did not result in any differences (p>0.05) in soybean meal TMEn, but phytase improved corn TMEn in 2.3% (p=0.004). In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Forced feeding; Phytase; Protease; Xylanase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100008
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Culture medium for amylase production by toxigenic fungi BABT
Figueira,Edson Luiz Zangrando; Hirooka,Elisa Yoko.
Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Fusarium moniliforme; Aspergillus flavus.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500003
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Different feeding habits influence the activity of digestive enzymes in freshwater fish Ciência Rural
Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia.
ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406
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Effect of temperature in Amylase and amyloglucosidase produced from english potato residue via fermentation in solid state. Repositório Alice
MELO, F. S.; ALVES, I. D. C.; BARBOSA, T. C. V.; CABRAL, L. M. C.; SANTOS, T. C..
bitstream/item/213058/1/galoa-proceedings-SINAFERM-SHEB-2019-106630.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Amyloglucosidase; Amylase; Enzymes; Fermentation.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122367
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Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds BABT
Katoch,Meenu; Salgotra,Aseem; Singh,Gurpreet.
This study aimed to screen the endophytic fungal species of ethano-medicinal plant Bacopa monnieri (L.) Pennell for their ability to produce antimicrobial substances against Bacillus subtilis, Pseudomonas aeroginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Candida albicans. Endophytes were also screened for their ability to produce amylase, cellulase, protease and lipase to evaluate their ecological role within the host plant. Twenty-six endophytes were isolated and seventeen were identified. All the isolated endophytes exhibited amylolytic activity. Lipolytic, cellulolytic, proteolytic activity was shown by 98, 28 and 31% isolates, respectively. Similarly, all the endophytes (100%) exhibited significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacopa monnieri; Antimicrobial; ITS 5.8S rDNA; Lipase; Amylase; Endophyte.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500714
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Enzimas digestivas exógenas na alimentação de juvenis de tambaqui. Repositório Alice
NUNES, R. da S.S.; CAVERO, B.A.S.; PEREIRA-FILHO, M.; ROUBACH, R..
O objetivo deste trabalho foi avaliar o efeito da adição na ração das enzimas digestivas exógenas amilase, lipase e protease sobre o desempenho de tambaqui, em três experimentos independentes. Em cada experimento foi avaliado o efeito da inclusão de uma enzima na ração em diferentes porcentagens: 0,0, 0,05, 0,1 e 0,2%, cada uma com quatro repetições, com 28 dias de duração. Foram realizadas biometrias do peso inicial, para homogeneização da amostra, e do peso final a fim de verificar o efeito dos tratamentos sobre o desempenho dos peixes. Em cada unidade experimental foram estocados dez peixes, alimentados duas vezes ao dia (8 e 14h) até a saciedade aparente, com ração comercial extrusada com 28% de proteína bruta, triturada, adicionada de enzima e depois...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Colossoma macropomum; Piscicultura; Nutrição de peixes; Amilase; Fish culture; Fish nutrition; Amylase; Lipase; Protease.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/118432
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Enzymatic programs for broilers BABT
Sorbara,José Otávio Berti; Murakami,Alice Eiko; Nakage,Eliane Saiuri; Piracés,Francisco; Potença,Alexandra; Guerra,Rafael Lachinski Holanda.
Two trials were conducted on floor pens to evaluate the performance of broilers fed with diets contained different carbohydrases enzyme programs at different ages. Trial 1- The body weight gain (BWG) was affected by the Negative Control (NC) treatment (P<0.05). However, when the birds were fed with the NC diet + Amilase (AMY)+Xilanase (XYL), the BWG improved and reached the same level as the Positive Control (PC). Trial 2- The birds received enzymes supplementation in the total phase and others only in the grower phase.The ME reduction by 120 kcal/kg increased the feed conversion ratio (FCR) (P<0.05) when compared to PC and none of the enzyme programs was able to recover the performance. It can be concluded that it is possible to supplement with AMY...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Carbohydrase; Enzymes; Amylase; Xylanase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700030
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Etude des effets de la sélection sur les gènes de l'amylase chez une population d'huître creuse Crassostrea gigas en fonction des conditions trophiques précoces ArchiMer
Sauvage, Christopher.
In natural population of the Pacific cupped oyster, there are differences between allelic and genotypic frequencies for the A and B amylase genes. Set up a synthetical population of C. gigas, in which whole alleles and genotypes are better represented than in wild population, was done. An investigation links between trophic conditions applied during larvae developpment (in nursery), allelic frequencies for both amylase genes, oyster's individual waight and by the selectivity of summer mortalities during their exposition on the estran has been done. As a result, following facts have been observed : - On one side, an influence of the trophic condition applied during the larvae development on genotypic frequencies of the gene B of the amylase - On the other...
Tipo: Text Palavras-chave: Crassostrea gigas; Amylase; Polymorphisme; Condition trophique; Crassostre gigas; Amylase; Polymorphim; Trophic condition.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00371/48185/48300.pdf
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