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Registros recuperados: 9
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Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent PAB
Poersch-Bortolon,Liane Balvedi; Scagliusi,Sandra Maria Mansur; Yamazaki-Lau,Elene; Bodanese-Zanettini,Maria Helena.
Abstract The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4ºC for 21 days; and 2-hydroxynicotinic acid, at 32ºC for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Albinism; Androgenesis; Doubled haploid; Isolated microspore culture; Recalcitrance.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101839
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Effect of temperature shock on soybean microspore embryogenesis BABT
Moraes,Ana Paula de; Bonadese-Zanettini,Maria Helena; Callegari-Jacques,Sídia Maria; Kaltchuk-Santos,Eliane.
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Soybean; Androgenesis; Symmetrical mitosis; Temperature shock; Stress.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400006
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Haplodiploid androgenetic breeding in oat: genotypic variation in anther size and microspore development stage Scientia Agricola
Cesaro,Taniela De; Baggio,Maria Irene; Zanetti,Silvia Andréia; Suzin,Marilei; Augustin,Lizete; Brammer,Sandra Patussi; Iorczeski,Edson Jair; Milach,Sandra Cristina Kothe.
Oat (Avena spp.) is poorly responsive to the haplodiploidization process, which leads to the production of homozygous lines in one step, increasing breeding efficiency. Androgenetic haploids in small grain cereal crops are obtained from microspores cultured at the mononucleate stage, which can be identified by the size of anthers. In order to identify the appropriate anther size for in vitro culture, microspore cytological analyses were made in Avena sativa cultivars UPF 7, UPF 18, UFRGS 14, Stout and Avena sterilis CAV 3361, cultivated in growth chamber under controlled light and temperature conditions. Variation was observed within and among genotypes for anther size at each microspore developmental stage and according to the position of spikelets in the...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Androgenesis; Anther culture; Biotechnology; Haploid breeding; Microspores.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000100016
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In vitro induction of haploid plants in unpollinated ovules, anther and microspore culture of Cucumis sativus National Institute of Agronomic Research
Suprunova, T.; Shmykova, N..
In order to increase the yield of haploid cucumber plants obtained from in vitro culture of unpollinated ovules, anther and microspore, we have studied several factors: the nature and concentration of plant growth regulators, the genotypes, the flower bud lengths, and the stages of microspore development. Among the different growth regulators tested, thidiazuron (TDZ) was the best for gynogenesis induction with an optimal concentration of 0.2 mg.L-1. Out of the ten evaluated genotypes, only cultivar ‘Gordion’ was able to develop the haploid plant through in vitro gynogenesis. Based on the histological studies we found that the best stages of the anther development for haploid induction are the late uninucleate microspore and the early bicellular pollen...
Tipo: Poster Palavras-chave: Cucumis sativus; Gynogenesis; Androgenesis; Haploid plant; Microspore.
Ano: 2008 URL: http://hdl.handle.net/2174/233
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Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains BABT
Rodrigues,Lia Rosane; Forte,Bianca de Camargo; Bodanese-Zanettini,Maria Helena.
In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androgenesis; Glycine max; Microspore culture; Microspore embryogenesis; Microspore isolation; Soybean.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002
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Morphological markers for microspore developmental stage in maize BABT
Moraes,Ana Paula de; Bered,Fernanda; Carvalho,Fernando Irajá Felix de; Kaltchuk-Santos,Eliane.
The use of maize in anther culture has been limited because only few genotypes presented a high androgenetic potential. Obtaining the proper stage of microspore development at culture initiation is one of the most crucial factors for success in the androgenesis. For Brazilian maize genotypes there are no studies reporting a correlation between cytological features and morphological parameters. In this study, morphological parameters were recorded and associated with cytological specific stages of the the microsporogenesis in two Brazilian maize genotypes that were sowed in different places (field and growing chamber). For both genotypes, the plants of the growing chamber presented a delay in development. Spikelets length and anther length/spikelet length...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androgenesis; Anther culture; Microsporogenesis; Zea mays.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500006
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Reproduction uniparentale et polyploidie induites chez la truite arc-en-ciel (Oncorhynchus mykiss) et chez les bivalves Crassostrea gigas, Ruditapes philippinarum et Chlamys varia ArchiMer
Diter, Alain.
Methods of gynogenesis, androgenesis and polyploidy induction were developed with the aim of genetic improvement in the rainbow trout, the Pacific oyster, the Manila clam and the black scallop. In the trout: · haploid androgenesis was induced by means of a pressure shock applied on diploid eggs, probably at the time of karyogamy. Diploid androgenetic individuals were directly produced from eggs fertilized with sperm from tetraploid male. · a heat shock treatment effective for suppressing the first egg mitosis was developed. This treatment allows the production of homozygous gynogenetic lines (checked using enzyme electrophoresis technique) from eggs inseminated with ultraviolet irradiated sperm. The treatment also induced tetraploidy in eggs normally...
Tipo: Text Palavras-chave: Méthode induction gynogenèse; Androgenèse; Polyploïdie; Oncorhynchus mykiss; Crassostrea gigas; Ruditapes philippinarum; Chlamys varis; Génétique; Methods of gynogenesis; Androgenesis; Polyploidy; Oncorhynchus mykiss; Crassostrea gigas; Ruditapes philippinarum; Chlamys varis; Genetic.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00210/32097/30553.pdf
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Segregation distortion in homozygous lines obtained via anther culture and maize doubled haploid methods in comparison to single seed descent in wheat (Triticum aestivum L.) Electron. J. Biotechnol.
Adamski,Tadeusz; Krystkowiak,Karolina; Kuczynska,Anetta; Mikolajczak,Krzysztof; Ogrodowicz,Piotr; Ponitka,Aleksandra; Surma,Maria; Slusarkiewicz-Jarzina,Aurelia.
Background The quality of wheat grain depends on several characteristics, among which the composition of high molecular weight glutenin subunits, encoded by Glu-1 loci, are the most important. Application of biotechnological tools to accelerate the attainment of homozygous lines may influence the proportion of segregated genotypes. The objective was to determine, whether the selection pressure generated by the methods based on in vitro cultures, may cause a loss of genotypes with desirable Glu-1 alleles. Results Homozygous lines were derived from six winter wheat crosses by pollination with maize (DH-MP), anther culture (DH-AC) and single seed descent (SSD) technique. Androgenetically-derived plants that originated from the same callus were examined before...
Tipo: Journal article Palavras-chave: Androgenesis; DH lines; HMW glutenin subunits; SSD technique; Winter wheat.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100002
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Towards the development of clonal lines in the European sea bass (Dicentrarchus labrax L.): application of uniparental reproduction techniques with an insight into sea bass eggs ArchiMer
Colleter, Julie.
Clonal lines are a powerful scientific tool for improved genetic characterization of organisms used in research. Inbred fish lines can be produced in only two generations using uniparental reproduction techniques. Androgenesis, achieved with variable success in several freshwater species, has been attempted in the European sea bass (Dicentrarchus labrax L), a marine fish of commercial and scientific interest. The low yields of progenies inheriting only the paternal genome after UV-irradiation of eggs led to considerations on the occurrence of UV screening compounds in pelagic eggs. Mycosporine-like amino acids and gadusol were found in many marine and freshwater organisms, but their occurrence in fish eggs was not clearly related to a behavioral pattern...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Lignées clonales; Androgenèse; Gynogenèse; MAAs; Gadusol; Marqueurs microsatellites; Homozygotie; Hétérozygotie; Taux de recombinaison.; Dicentrarchus labrax; Clonal lines; Androgenesis; Gynogenesis; MAAs; Gadusol; Microsatellite markers; Homozygosity; Heterozygosity; Recombination rate..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00300/41138/40326.pdf
Registros recuperados: 9
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