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A comparative proteomic study of white and black glutinous rice leaves Electron. J. Biotechnol.
Phonsakhan,Wipada; Kong-Ngern,Kanlaya.
Background Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5...
Tipo: Journal article Palavras-chave: 2D-PAGE; Anthocyanin; LC-MS/MS; Oryza sativa L; Proteomics.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100006
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Accumulation des anthocyanes et caroténoïdes dans les abricots rouges National Institute of Agronomic Research
Bureau, Sylvie; Gouble, Barbara; Grotte, Maggy; Ginies, Christian; Reich, Maryse; Reling, Patrice; Bogé, Marielle; Clauzel, Guy; Audergon, Jean-Luc.
Tipo: Working Paper Palavras-chave: Anthocyanin; Carotenoid; Apricot; Secondary metabolite; Anthocyane; CaroténoÏde; Abricot; Métabolite secondaire.
Ano: 2008 URL: http://hdl.handle.net/2174/174
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Alternatives to increase the red color of the peel in ‘Daiane’ and ‘Venice’ apples Rev. Bras. Frutic.
Fenili,Cristhian Leonardo; Petri,José Luiz; Steffens,Cristiano André; Martin,Mariuccia Schlichting de; Amarante,Cassandro Vidal Talamini do; Heinzen,Angélica Schmitz.
Abstract This study aimed evaluate the effect of biostimulants, foliar fertilizers and ethephon on the red color in the peel of ‘Daiane’ and ‘Venice’ apples. The experiments were conducted in Caçador/SC, during 2015/2016 and 2016/2017 seasons. In 2015/2016, the treatments were: Control; Biostimulant I; Foliar fertilizer I + Foliar fertilizer II; Biostimulant II and KCl. In 2016/2017, was included the Ethephon treatment. In 2016/2017, Biostimulant I, Foliar fertilizer I + Foliar fertilizer II and Biostimulant II increased the percentage of ‘Daiane’ and ‘Venice’ apples with greater red peel cover, without affecting the quality and maturity of the fruits. In both cultivars, ethephon was treatment that promoted a higher percentage of apples in the category...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malus domestica; Anthocyanin; Biostimulants; Foliar fertilizer; Fruit quality.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200303
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Annual Variations in the Anthocyanin Contents of Blueberry Fruit Grown in Hokkaido OAK
宮下, 淳一; 西, 繁典; 齋藤, 優介; 小疇, 浩; 弘中, 和憲; 小嶋, 道之.
アントシアニンを多く含有する果物は、健康機能性や色素作用により、非常に高く評価されている。2003 年の冷害年を含む2002~2004 年の摘期に収穫された6 品種の粒大の異なるブルーベリー果実を用いて、それらに含まれるアントシアニン含量を比較検討した。収穫年度により、果実重量あたりのアントシアニン含量は顕著に異なっていた。また、小~中粒果実 (ウェイマウス、ノースランド、ジューン、パトリオット)のアントシアニン含量は、栽培環境に影響を受けにくいが、大粒果実 (ハーバート、ブルーレイ)のそれは栽培環境に影響を受け易いことが示唆された。特に冷害年である2003年のノースランド果実のアントシアニンおよびポリフェノール含量は、他年度のそれらよりも顕著に高く、低温環境においてアントシアニン合成が増加する特徴を持っていると推定された。また、2004年、5 月と6 月の平均気温が長沼町よりも若干低い砂原町で生育したブルーベリー果実の粒大は小さかったが、果実100g 当りのアントシアニン量は高かった。今後、粒大が大きく、アントシアニン含量の高い品種の選抜・育成が期待される。
Palavras-chave: ブルーベリー; 品種選抜; アントシアニン; ポリフェノール; Blueberry; Anthocyanin; Variety selection; Antioxidant activity; Polyphenol; 抗酸化活性.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1607
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Anthocyanin characteristics of wines in Vitis germplasms cultivated in southern China Ciênc. Tecnol. Aliment.
CHENG,Guo; ZHOU,Si-Hong; WEN,Ren-De; Xie,Tai-Li; HUANG,Yu; YANG,Ying; GUAN,Jing-Xi; XIE,Lin-Jun; ZHANG,Jin.
Abstract The anthocyanin profiles and CIELAB color values of nine wines in Vitis germplasms from southern China were compared. The results showed that the anthocyanin composition of wines from one hybrid between V. vinifera and V. labrusca (‘Moldova’), two V. labrusca varieties (‘Conquistador’ and ‘Saint-Croix’), one V. quinquangularis variety (‘Yeniang No.2’), one hybrid between V. quinquangularis and V. vinifera (‘NW196’), one V. davidii variety (‘Xiangniang No.1’) and one V. rotundifolia variety (‘Noble’) were dominated by anthocyanidin 3,5-O-diglucosides. All these were quite different from V. vinifera wines (‘Cabernet Sauvignon’ and ‘Marselan’), which were characterized by the monoglucoside and pyranoanthocyanins. 3',4',5'-substituted anthocyanins...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanin; Wine; HPLC-MS; V. germplasm; Variety.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000300513
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Anthocyanins from blackberry (Rubus fructicosus L.) impregnated in yam bean (Pachyrhizus erosus (L.) Urb.) by osmotic dehydration Ciênc. Tecnol. Aliment.
GRAJALES-LAGUNES,Alicia; CABRERA-RUIZ,Luis; GUTIÉRREZ-MICELI,Federico; RUIZ-CABRERA,Miguel Angel; DENDOOVEN,Luc; ABUD-ARCHILA,Miguel.
Abstract Yam bean (Pachyrhizus erosus (L.) Urb.) tuber has a low carbohydrate content and contains several aminoacids, but contains few active compounds, such as vitamins and antioxidants. Anthocyanins are antioxidants found in Rubus fructicosus L. The osmotic drying is a technique that can be used to impregnate antioxidants in a matrix, so the objective of this study was to impregnate yam bean tuber slices with anthocyanin. The effect of temperature (40, 50 and 60oC), sucrose content (40, 50 and 60oBrix) and vacuum pulse (0, 300 and 600 mbar) on water loss (WL), solid gain (SG), color changes (ΔΕ) and anthocyanin concentration (ΔA) during osmotic drying of yam bean slices was determined. Polynomial equations were used to determine changes in WL, SG, ΔΕ...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanin; Impregnation; Optimization; Osmotic drying; Yam bean.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000400922
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Anthocyanins, pigment stability and antioxidant activity in jabuticaba [Myrciaria cauliflora (Mart.) O. Berg] Rev. Bras. Frutic.
Lima,Annete de Jesus Boari; Corrêa,Angelita Duarte; Saczk,Adelir Aparecida; Martins,Mariana Pereira; Castilho,Rachel Oliveira.
Different solvents were evaluated for the extraction of jabuticaba anthocyanin pigments, identifying, quantifying and verifying the stability of the anthocyanins, as well as the conduction of three antioxidant activity assays and determination of the vitamin C levels. The maceration with ethanol acidified with HCl 1.5 mol L-1 (85:15) provides better pigment extraction and stability. The skin is anthocyanin rich, presenting 1.59 and 2.06 g 100 g-1 of dry matter in the Paulista and Sabará varieties, respectively. Cyanidin 3-glucoside is the majority pigment of the skins, followed by delphinidin 3-glucoside. The highest level of vitamin C was found in the skins and seeds of both varieties. It was verified that the skins, presented more antioxidant activity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jabuticaba; Anthocyanin; Method of extraction; Vitamin C; Antioxidant activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000300023
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Characterization of açaí (E. oleracea) fruits and its processing residues BABT
Pessoa,José Dalton Cruz; Arduin,Marcos; Martins,Maria Alice; Carvalho,José Edmar Urano de.
The aim of this work was to study the source of the açaí residue for its possible commercial applications by characterizing the fruit (fresh and dry mass), and performing an anatomic study of the pericarp from which were identified the origin of the anthocyanins, and fatty acids, and fibers; also the vascular system, its fibers constituents and fibrils were characterized. It was concluded that anthocyanins were located on the epiderm and external parenchyma, and that solids retained on the sieve come from the sclerenchyma, and that the fatty acids come from the storage parenchyma. The vascular tissue was formed by the fibers around 20 mm length. The length distribution of the fibrils had a mean length of 580µm.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fiber; Fibril; Composite; Fatty acid; Anthocyanin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600022
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Comparison of radiosensitivity response to acute and chronic gamma irradiation in colored wheat Genet. Mol. Biol.
Hong,Min Jeong; Kim,Dae Yeon; Ahn,Joon-Woo; Kang,Si-Yong; Seo,Yong Weon; Kim,Jin-Baek.
Abstract We aimed to investigate the biological responses induced by acute and chronic gamma irradiation in colored wheat seeds rich in natural antioxidants. After acute and chronic irradiation, the phenotypic effects on plant growth, germination rate, seedling height, and root length were examined, and the biochemical changes were investigated by analyzing the expression of antioxidant enzyme-related genes, antioxidant enzyme activities, and total antioxidant capacity. High dosages of chronic radiation reduced plant growth compared with the controls. Electron spin resonance measurement and 2,2-diphenyl-1-picrylhydrazyl activity analysis showed lower amount of free radicals in colored wheat seeds on chronic irradiation with low dosage of gamma rays...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute irradiation; Chronic irradiation; Wheat; Electron spin resonance; Anthocyanin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400611
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Effect of processing conditions of hot pressurized solvent extraction in batch reactor on anthocyanins of purple field corn CIGR Journal
Muangrat, Rattana; Jampatong, Sansern; Saengcharoenrat, Phanat.
Total anthocyanin content of the dried kernel and dried cob of purple field corn was investigated under hot pressurized solvent extraction conditions. The highest total anthocyanin content of 492.51 µg cyanidin-3-glucoside/g dry weight of sample was obtained from dried kernel extraction using water-ethanol ratio 1:3 as solvent at sample-solvent ratio 1:8 and extraction temperature of 80 oC. For the dried cob extraction, the highest total anthocyanin content obtained was 1890.49 µg cyanidin-3-glucoside/g dry weight of sample using water-ethanol ratio 1:1 as solvent at sample-solvent ratio 1:8, and extraction temperature of 100 oC. The extraction was carried out at pressure of 0.20 MPa and 15 min with N2 purging. Additionally, the antioxidant activities...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanin; Purple field corn; Hot pressurized solvent extraction; Antioxidant activities.
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4231
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Effects of Anthocyanin-rich Colored Potato Flakes on Lipid Oxidation, Instrumental Color Evaluation and Sensory Characteristics in Cooked Pork Sausages OAK
JAYAWARDANA, Barana C.; YANAGIHARA, Maki; HAN, Kyu-Ho; FUKUSHIMA, Michihiro; SEKIKAWA, Mitsuo; SHIMADA, Kenichiro; 韓, 圭鎬; 福島, 道広; 関川, 三男; 島田, 謙一郎.
We investigated lipid oxidation, instrumental color evaluation, and sensory characteristics in cooked pork sausages containing anthocyanin rich colored potato (Solanum tuberosam cv. Shadow Queen) flakes (CPF). According to the thiobarbituric acid reactive substance values, addition of 2% CPF suppressed lipid oxidation (P < 0.05) by 80% compared to the control. The antioxidant activity of 2% CPF was comparable to the synthetic antioxidant butylated hydroxytoluene (0.1%) in cooked pork sausages. The L, a* values and odor, flavor, taste, texture and overall sensory quality did not significantly differ between control and 2% CPF. Overall, cooked pork sausage with 2% CPF was as acceptable to consumers as the control. Thus, the present study indicated that...
Palavras-chave: Anthocyanin; Colored potato flake; Lipid oxidation; Natural antioxidant; Pork sausage.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3842
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Effects of bagging or the combination of umbrella and bag treatments on anthocyanin accumulation in the berry skin of ‘Kyoho’ (Vitis labruscana) Grape Ciênc. Tecnol. Aliment.
ZHOU,Si-Hong; GUO,Rong-Rong; WEI,Rong-Fu; LIU,Jin-Biao; YU,Huan; SHI,Xiao-Fang; ZHANG,Ying; XIE,Tai-Li; CHENG,Guo.
Abstract The object of this study was to investigate the influence of bagging or the combination of umbrella and bags on berry composition, especially anthocyanin accumulation, in ‘Kyoho’ (V. labruscana) berry skins. Five different colors and bag materials commonly used in production were chosen for this study: white, green, yellow, nonwoven and transparent, and the umbrellas were made of plastic material. The composition and content of anthocyanins in the berry skin of ‘Kyoho’ were analyzed by HPLC-MS. The results showed that bagging or a combination of umbrella and bags on anthocyanin concentration in ‘Kyoho’ grapes showed inconsistent effects that depended on the colors and materials of the bags and the use of an umbrella or not. Compared with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bagging; Umbrella; Anthocyanin; ‘Kyoho’; Grape.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000200394
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Effects of bagging or the combination of umbrella and bag treatments on anthocyanin accumulation in the berry skin of ‘Kyoho’ (Vitis labruscana) Grape Ciênc. Tecnol. Aliment.
ZHOU,Si-Hong; GUO,Rong-Rong; WEI,Rong-Fu; LIU,Jin-Biao; YU,Huan; SHI,Xiao-Fang; ZHANG,Ying; XIE,Tai-Li; CHENG,Guo.
Abstract The object of this study was to investigate the influence of bagging or the combination of umbrella and bags on berry composition, especially anthocyanin accumulation, in ‘Kyoho’ (V. labruscana) berry skins. Five different colors and bag materials commonly used in production were chosen for this study: white, green, yellow, nonwoven and transparent, and the umbrellas were made of plastic material. The composition and content of anthocyanins in the berry skin of ‘Kyoho’ were analyzed by HPLC-MS. The results showed that bagging or a combination of umbrella and bags on anthocyanin concentration in ‘Kyoho’ grapes showed inconsistent effects that depended on the colors and materials of the bags and the use of an umbrella or not. Compared with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bagging; Umbrella; Anthocyanin; ‘Kyoho’; Grape.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005028111
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Estimates of genetic parameters of pigments and agronomic traits in green and purple lettuce Ciência e Agrotecnologia
Oliveira,Alisson Henrique Gama de; Maciel,Gabriel Mascarenhas; Siquieroli,Ana Carolina Silva; Jacinto,Ana Carolina Pires; Silveira,Aline José da; Silva,Ernani Clarete da.
ABSTRACT Understanding genetics and the gene interactions involved in the expression of certain characteristics becomes a determining factor for better exploration of its use. The development of breeding programs that combine one or more desirable trait into a single cultivar is directly impacted by this lack of knowledge of the genetic bases involved. The objective of the present study was to estimate the genetic parameters involved in pigment expression and agronomic characteristics in lettuce. Parental evaluations of lettuce, P1 (green) and P2 (purple) parental generations, the segregating generations F1 and F2, and the backcrosses Bc1 and Bc2 originating from the UFU-Crespa199#1#1 x UFU-Albina#2 crossing were evaluated. The chlorophyll and anthocyanin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanin; Genetic interactions; Segregating generations..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100226
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Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes Electron. J. Biotechnol.
Naing,Aung Htay; Ai,Trinh Ngoc; Jeon,Su Min; Park,Kyeung Il; Lim,Ki Byung; Kim,Chang Kil.
Background Several MYB genes belonging to R2R3 MYB transcription factors have been used in several plant species to enhance anthocyanin production, and have shown various expression or regulation patterns. This study focused on the effect of ectopic expression of an RsMYB1 isolated from radish (Raphanus sativa) on chrysanthemum cv. ‘Shinma'. Results The RT-PCR results confirmed that RsMYB1 regulated the expression of three key biosynthetic genes (CmF3H, CmDFR, and CmANS) that are responsible for anthocyanin production in transgenic chrysanthemum, but were not detected in the non-transgenic line. In all transgenic plants, higher expression levels of key biosynthetic genes were observed in flowers than in leaves. However, the presence of RsMYB1 in...
Tipo: Journal article Palavras-chave: Anthocyanin; Chrysanthemum; Morphology; RT- PCR; Transcription factor.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500006
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Identification of roselle varieties through simple discriminating physicochemical characteristics using multivariate analysis Ciênc. Tecnol. Aliment.
KANE,Alé; ACHIR,Nawel; CISSE,Mady; PALLET,Dominique; SAKHO,Mama; DORNIER,Manuel.
Abstract The objective of this work is to study the feasibility of a more objective and rigorous classification of the calices of Hibiscus sabdariffa based on their physicochemical profile. To do so, 19 analyses were carried out on 4 varieties of calices cultivated in Senegal: Vimto, Koor, Thaï and CLT92. Principal component analysis results showed that 15 physicochemical and biochemical parameters could be potentially used to discriminate the varieties of calices. Polyphenolic and anthocyanin contents were anti-correlated to protein content and could be used to differentiate the Vimto/CLT92 and the Koor/Thaï varieties. Within these two clusters, pH and lipid content could discriminate each variety. Finally, factorial discriminant analysis showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hibiscus sabdariffa L.; Discriminant analysis; Colour; Anthocyanin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000200321
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Intensidad luminosa y ácido giberélico en la producción de anturio para flor de corte. Colegio de Postgraduados
Rangel Estrada, Sandra Eloisa.
El anturio (Anthurium andraeanum Linden ex André) es una planta tropical que en su hábitat natural crece bajo la sombra de árboles y sus inflorescencias vistosas con larga vida en florero la ubican dentro de las más caras en el mercado de flores de corte en México y a escala mundial. En nuestro país se considera una especie ornamental no tradicional por lo que se desconocen muchos aspectos sobre los requerimientos de su cultivo. En la presente investigación, realizada en Fortín de las Flores, Veracruz, se estudiaron los efectos producidos por tres intensidades luminosas (256, 290 y 364 μmol m-2 s-1) proporcionadas por mallas sombra comerciales y la aplicación foliar del ácido giberélico (AG3) en el desarrollo, rendimiento y calidad de las inflorescencias...
Palavras-chave: Anthurium andraeanum; Antocianinas; Flor de corte; Vida florero; Intensidad luminosa; Anthocyanin; Cut flower; Vase life; Light intensity; Doctorado; Fisiologia vegetal.
Ano: 2011 URL: http://hdl.handle.net/10521/520
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Intensidad luminosa y ácido giberélico en la producción de anturio para flor de corte. Colegio de Postgraduados
Rangel Estrada, Sandra Eloisa.
El anturio (Anthurium andraeanum Linden ex André) es una planta tropical que en su hábitat natural crece bajo la sombra de árboles y sus inflorescencias vistosas con larga vida en florero la ubican dentro de las más caras en el mercado de flores de corte en México y a escala mundial. En nuestro país se considera una especie ornamental no tradicional por lo que se desconocen muchos aspectos sobre los requerimientos de su cultivo. En la presente investigación, realizada en Fortín de las Flores, Veracruz, se estudiaron los efectos producidos por tres intensidades luminosas (256, 290 y 364 μmol m-2 s-1) proporcionadas por mallas sombra comerciales y la aplicación foliar del ácido giberélico (AG3) en el desarrollo, rendimiento y calidad de las inflorescencias...
Palavras-chave: Anthurium andraeanum; Antocianinas; Flor de corte; Vida florero; Intensidad luminosa; Anthocyanin; Cut flower; Vase life; Light intensity; Doctorado; Fisiologia vegetal.
Ano: 2011 URL: http://hdl.handle.net/10521/520
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Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) Electron. J. Biotechnol.
Zhao,Da Qiu; Han,Chen Xia; Ge,Jin Tao; Tao,Jun.
Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower...
Tipo: Journal article Palavras-chave: Anthocyanin; Expression; Glucosyltransferase; Herbaceous peony.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600009
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Mycorrhizal inoculation affects the phytochemical content in strawberry fruits Agronomy
Cecatto, Ana Paula; Ruiz, Fátima Martínez; Calvete, Eunice Oliveira; Martínez, Jesús; Palencia, Pedro.
The aim of this research was to evaluate the effect of the inoculation date of arbuscular mycorrhizal fungi on the fruit quality and the content of phytochemicals in a strawberry soilless growing system. The experiment was performed in Huelva (Spain) and was conducted in a greenhouse on the La Rábida Campus of Huelva University under natural light and temperature from October 2013 to June 2014. Three short-day strawberry cultivars (‘Splendor’, ‘Sabrina’ and ‘Fortuna’) were grown in polyethylene bags filled with coconut fibres. Randomized block design, with 3 repetitions and factorial arrangement (3 cultivars x 3 treatments), was established. Each replicate consisted of one bag with 12 plants supporting structures at 40 cm height. The treatments were: T1 =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 Fragaria x ananassa Duch.; Anthocyanin; Phenolics; Ascorbic acid Fitotecnia.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/27932
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