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Registros recuperados: 19
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A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D.
Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this...
Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf
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Action de l'acétate de tributyle-étain sur les oeufs et les larves D de deux mollusques d'intérêt commercial : Crassostrea gigas (Thunberg) et Mytilus galloprovincialis (Lmk) ArchiMer
Robert, Rene; His, Edouard.
The Tributyl tin acetate, a component of certain antifouling paints, has deleterious effects on eggs and D shaped larvae from Crassostrea gigas at 5 p.p.b. (HIS & ROBERT, 1980). Here we have tested concentrations as low as 1 p.p.b. on eggs and on 24 hours old larvae both from Crassostrea gigas and Mytilus galloprovincialis. The same results are got ; this product kills embryos and larvae in a few days ; it prevents growth of 24 hours old larvae exposed to this toxic. Mussels larvae seem to be less sensitive.
Tipo: Text Palavras-chave: Antifouling; Larves; Pollution; Toxicité; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis; Antifouling; Larvae; Pollution; Toxicity; Bivalvia; Crassostrea gigas; Mytilus galloprovincialis.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00037/14862/12185.pdf
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Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment ArchiMer
Le Norcy, Tiffany; Niemann, Hendrik; Proksch, Peter; Linossier, Isabelle; Vallee-rehel, Karine; Hellio, Claire; Fay, Fabienne.
Dibromohemibastadin-1 (DBHB) is an already known potent inhibitor of blue mussel phenoloxidase (which is a key enzyme involved in bioadhesion). Within this study, the potentiality of DBHB against microfouling has been investigated. The activity of DBHB was evaluated on key strains of bacteria and microalgae involved in marine biofilm formation and bioassays assessing impact on growth, adhesion and biofilm formation were used. To assess the efficiency of DBHB when included in a matrix, DBHB varnish was prepared and the anti-microfouling activity of coatings was assessed. Both in vitro and in situ immersions were carried out. Confocal Laser Scanning Microscopy (CLSM) was principally used to determine the biovolume and average thickness of biofilms developed...
Tipo: Text Palavras-chave: Antifouling; Biodegradable coating; Hemibastadin; Sponges; Marine bacteria; Microalgae.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00616/72812/72626.pdf
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Biomimetic Approaches for the Development of New Antifouling Solutions: Study of Incorporation of Macroalgae and Sponge Extracts for the Development of New Environmentally-Friendly Coatings ArchiMer
Sánchez-lozano, Ilse; Hernández-guerrero, Claudia Judith; Muñoz-ochoa, Mauricio; Hellio, Claire.
Biofouling causes major economic losses in the maritime industry. In our site study, the Bay of La Paz (Gulf of California), biofouling on immersed structures is a major problem and is treated mostly with copper-based antifouling paints. Due to the known environmental effect of such treatments, the search for environmentally friendly alternatives in this zone of high biodiversity is a priority to ensure the conservation and protection of species. The aim of this work was to link chemical ecology to marine biotechnology: indeed, the natural defense of macroalgae and sponge was evaluated against biofoulers (biofilm and macrofoulers) from the same geographical zone, and some coatings formulation was done for field assays. Our approach combines in vitro and...
Tipo: Text Palavras-chave: Adhesion; Algae; Antifouling; Biofilm; Extracts; Field assays; Sponges; Haliclona caerulea; Laurencia gardneri; Sargassum horridum; Ircinia sp.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00589/70074/68047.pdf
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Cultured heart cells from oyster: an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin ArchiMer
Droguet, Mickael; Devauchelle, Nicole; Pennec, Jean-pierre; Quinn, Brian; Dorange, Germaine.
European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10(-6) M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4,...
Tipo: Text Palavras-chave: Oyster; Heart cells; Flow cytometry; Tributyltin; Toxicity; Antifouling.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00089/20032/17688.pdf
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Development of environmentally friendly antifouling paints using biodegradable polymer and lower toxic substances ArchiMer
Carteau, David; Vallee-rehel, Karine; Linossier, Isabelle; Quiniou, Francoise; Davy, Romain; Compere, Chantal; Delbury, Maxime; Fay, Fabienne.
The development of new antifouling coatings with respect to the marine environment is actually crucial. The aim of the present work is to concept an erodible paint formulated with biodegradable polyester as binders and which combines two modes of prevention: chemical and physical repelling of biofouling. This system is principally dedicated to disturb durable settlement of microfouling. Each component was chosen according to its specific properties: chlorhexidine is a bisdiguanide antiseptic with antibacterial activity, zinc peroxide is an inorganic precursor of high instable entities which react with seawater to create hydrogen peroxide, Tween 85 is a non ionic surfactant disturbing interactions between colonizing organisms and surface. Obtained results...
Tipo: Text Palavras-chave: Environmental coatings; Antifouling; Active substances; Microfouling.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00168/27923/27273.pdf
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Effects of commercial enzymes on the adhesion of a marine biofilm-forming bacterium ArchiMer
Leroy, C.; Delbarre Ladrat, Christine; Ghillebaert, F.; Compere, Chantal; Combes, D..
The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidino-2-phenylindole). Savinase (subtilisin) was the most effective hydrolase in both the prevention of bacterial adhesion and the removal of adhered bacteria. However, some enzymatic preparations tested such as Amano protease were not only ineffective but also increased the number of adhered bacterial cells. Enumeration using epifluorescence microscopy of CTC...
Tipo: Text Palavras-chave: Marine biofilm; Antifouling; Pseudoalteromonas; Enzymes; Adhesion.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6140.pdf
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Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds ArchiMer
Réveillon, Damien; Tunin-ley, Alina; Grondin, Isabelle; Othmani, Ahlem; Zubia, Mayalen; Bunet, Robert; Turquet, Jean; Culioli, Gérald; Briand, Jean-françois.
Marine microalgae and cyanobacteria have largely been studied for their biotechnological potential and proved their ability to produce a wide array of bioactive molecules. We investigated the antifouling potential of unexplored benthic tropical microalgae using anti-adhesion and toxicity bioassays against two major micro- and ma crobiofoulers, namely bacteria and barnacles. Fifty strains belonging to six phyla [Cyanobacteria, Miozoa (Dinoflagellata), Bacillariophyta, Cryptophyta, Rhodophyta and Haptophyta] were isolated from southwestern Islands of the Indian Ocean. They were chosen in order to represent as much as possible the huge biodiversity of such a rich tropical ecosystem. The associated chemodiversity was highlighted by both NMR- and LC-MS-based...
Tipo: Text Palavras-chave: Microalgae; Antifouling; Bioassay; Chemodiversity; Bioprospecting; Metabolomics.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00454/56608/58333.pdf
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Factors influencing the deterioration of the carapace surface during the moult cycle of Carcinus maenas (Linnaeus, 1758) Naturalis
Greco, G.; Faimali, M.; Davenport, J..
During its life cycle Carcinus maenas (Decapoda, Portunidae) goes through several phases of the moulting process (ecdysis) which allow it to grow despite having a rigid, non-living outer surface. As an individual approaches ecdysis, the exoskeletal calcium is solubilized (decalcification) from the shell and transferred through the integumentary epithelium to the blood, where much of it is transported to cells, tissues and organs of temporary storage where it is later mobilized for deposition into the new exoskeleton. We hypothesised that the decalcification process caused deterioration of the features and structures (e.g. tubercles and setae) characterizing the crab carapace, but that this can be reversed by moulting. We also tested whether wear and tear...
Tipo: Article / Letter to the editor Palavras-chave: Antifouling; Crustaceans; Epibiosis; Green crab; Moulting process; SEM; 42.74.
Ano: 2014 URL: http://www.repository.naturalis.nl/record/480051
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From Ecology to Biotechnology, Study of the Defense Strategies of Algae and Halophytes (from Trapani Saltworks, NW Sicily) with a Focus on Antioxidants and Antimicrobial Properties ArchiMer
Messina, Concetta Maria; Renda, Giuseppe; Laudicella, Vincenzo Alessandro; Trepos, Rozenn; Fauchon, Marilyne; Hellio, Claire; Santulli, Andrea.
This study aimed at the characterization of the antioxidant power of polyphenol extracts (PE) obtained from the algae Cystoseira foeniculacea (CYS) (Phaeophyta) and from the halophyte Halocnemum strobilaceum (HAL), growing in the solar saltworks of western Sicily (Italy), and at the evaluation of their anti-microfouling properties, in order to correlate these activities to defense strategies in extreme environmental conditions. The antioxidant properties were assessed in the PE based on the total antioxidant activity test and the reducing power test; the anti-microfouling properties of the two PE were evaluated by measuring the growth inhibition of marine fish and shellfish pathogen bacteria as well as marine surface fouling bacteria and microalgae exposed...
Tipo: Text Palavras-chave: Polyphenols; Defenses; Anti-oxidants; Antifouling; Anti-microbial; Cystoseira foeniculacea; Halocnemum strobilaceum.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00605/71675/70090.pdf
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Glycoglycerolipids From Sargassum vulgare as Potential Antifouling Agents ArchiMer
Plouguerné, Erwan; De Souza, Lauro M.; Sassaki, Guilherme L.; Hellio, Claire; Trepos, Rozenn; Da Gama, Bernardo A. P.; Pereira, Renato Crespo; Barreto-bergter, Eliana.
Unraveling new environmentally friendly antifouling (AF) agents is one of the major quests currently facing marine biotechnology. Marine macroalgae represent a rich source of new compounds with promising biological properties, including AF activity, but most of the macroalgal compounds studied to date are terpenoids or polyphenolics. The aim of this work was to investigate the possible AF role played by a usually neglected class of marine natural products: glycolipids (monogalactosyldiacylglycerols – MGDG, digalactosyldiacylglycerols – DGDG and sulfoquinovosyldiacylglycerols – SQDG) isolated from the Phaeophyceae Sargassum vulgare collected along the coast of south-eastern Brazil. Among the 18 extracts, fractions and sub-fractions tested, 3 sub-fractions...
Tipo: Text Palavras-chave: Sargassum vulgare; Glycoglycerolipids; Antifouling; Microfouling; Macroalgae.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00618/73053/72168.pdf
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Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Compere, Chantal; Combes, D.
Aims: The nature of exopolymers involved in the adhesion of a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand the antifouling potential of subtilisin. Methods and Results: The exopolymers of D41 produced by fermentation were analysed by FTIR and SDS-PAGE showing the presence of polysaccharides, glycoproteins and proteins. A high content of proteins was detected both in soluble and capsular fractions. The microscopic observations of fluorescamine and calcofluor stained adhered D41 indicated mainly the presence of proteins in exopolymers produced during adhesion. Subtilisin, the broad spectrum protease, tested in natural sea water and in polystyrene microplates showed that antifouling activity was...
Tipo: Text Palavras-chave: Subtilisin; Pseudoalteromonas; Exopolymer; Capsular protein; Biofilm; Antifouling.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4624.pdf
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Investigation of the antifouling constituents from the brown alga Sargassum muticum (Yendo) Fensholt ArchiMer
Bazes, Alexandra; Silkina, Alla; Douzenel, Philippe; Fay, Fabienne; Kervarec, Nelly; Morin, Dimitri; Berge, Jean-pascal; Bourgougnon, Nathalie.
One of the most promising alternatives to toxic heavy metal-based paints is offered by the development of antifouling coatings in which the active ingredients are compounds naturally occurring in marine organisms and operating as natural antisettlement agents. Sessile marine macroalgae are remarkably free from settlement by fouling organisms. They produce a wide variety of chemically active metabolites in their surroundings, potentially as an aid to protect themselves against other settling organisms. In this study, a dichloromethane extract from the brown seaweed Sargassum muticum was tested in situ and, after 2 months of immersion, showed less fouling organisms on paints in which the extract was included, compared to paints containing only copper after 2...
Tipo: Text Palavras-chave: Sargassum muticum; Palmitic acid; Dioctyl phthalate; Dichloromethane extract; Brown algae; Antifouling.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6601.pdf
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Lutte contre les salissures marines : approche par procédés enzymatiques. ArchiMer
Leroy, Celine.
Fouling on marine underwater surfaces causes critical and economic problems such as important material biodamages and industrial performances reduction. We chose to test antifouling potential of enzymatic commercial preparations like hydrolases (proteases, glycosidases and lipases) in order to inhibit the first fouling adhesion step: bacterial biofilm formation. An evaluation test of antifouling properties onto marine bacterial adhesion was designed using a mono-incubation of Pseudoalteromonas sp. D41 in microtiter plate and in sterile natural sea water. This test was adapted to screen agents for bacterial adhesion removal or inhibition activities and allowed to test enzymatic preparations toxicity on non adhered bacteria. Inhibition rates according to...
Tipo: Text Palavras-chave: Fouling; Adhesion; Antifouling; Hydrolases; EPS; DAPI; Microtiter plate; Screening; Pseudoalteromonas sp.; Marine biofilm; Salissure marine; Adhésion; Antisalissure; Hydrolases; EPS; DAPI; Microplaque; Criblage; Pseudoalteromonas sp.; Biofilm marin.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-1099.pdf
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Marine bacteria from the Gulf of California with antimicrofouling activity against colonizing bacteria and microalgae ArchiMer
Sanchez-rodriguez, Diana Elizabeth; Ortiz-aguirre, Ismael; Aguila-ramirez, Ruth Noemi; Guadalupe Rico-virgen, Erika; Gonzalez-acosta, Barbara; Hellio, Claire.
One way of reducing the input of pollutants into the marine environment is to enforce the use of non-toxic antifouling paints in marine protected areas. Thus, the purpose of this study was to detect marine microorganisms that secrete inhibitory substances against bacteria and microalgae to avoid biofouling on manmade structures in La Paz bay, B.C.S., Mexico. The inhibitory potential of 125 bacteria was evaluated against biofilm-forming bacteria. Crude extracts were obtained with methanol and ethyl acetate from 16 bacterial strains that exhibited antagonistic and antibacterial activity in a preliminary screening. Antibacterial and antimicroalgal assays were performed using crude extracts, the minimum inhibitory concentration (MIC) was determined. The...
Tipo: Text Palavras-chave: Epibionts; Extracts; Shewanella; Staphylococcus; Biofouling; Antifouling.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00616/72762/72271.pdf
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Microalgal sensitivity varies between a diuron-resistant strain and two wild strains when exposed to diuron and irgarol, alone and in mixtures ArchiMer
Dupraz, Valentin; Coquille, Nathalie; Menard, Dominique; Sussarellu, Rossana; Haugarreau, Larissa; Stachowski-haberkorn, Sabine.
A wild strain of Chaetoceros calcitrans and wild and diuron-resistant strains of Tetraselmis suecica, were exposed to the PSII inhibitor herbicides diuron and irgarol, individually and in mixtures. The effects of three concentrations of diuron and irgarol and four binary mixtures were evaluated on doubling time, relative reactive oxygen species and lipid content by flow cytometry, and on photosynthetic efficiency by pulse amplitude modulated fluorescence. In both wild strains, significant effects were observed for each molecule at the highest concentration tested: at irgarol 0.5 μg L−1, C. calcitrans was shown to be more sensitive than T. suecica (+52% and +19% in doubling time, respectively), whereas at diuron 5 μg L−1, T. suecica was more affected...
Tipo: Text Palavras-chave: Microalgae; Herbicides; Antifouling; Irgarol; Diuron; Diuron resistance.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00319/43047/42658.pdf
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Natural antifouling compound production by microbes associated with marine macroorganisms: A review Electron. J. Biotechnol.
Satheesh,Sathianeson; Ba-akdah,Mohammad Abdulaziz; Al-Sofyani,Abdulmohsin A.
In the marine environment, all hard surfaces including marine macroorganims are colonized by microorganisms mainly from the surrounding environment. The microorganisms associated with marine macroorganisms offer tremendous potential for exploitation of bioactive metabolites. Biofouling is a continuous problem in marine sectors which needs huge economy for control and cleaning processes. Biotechnological way for searching natural product antifouling compounds gained momentum in recent years because of the environmental pollution associated with the use of toxic chemicals to control biofouling. While, natural product based antifoulants from marine organisms particularly sponges and corals attained significance due to their activities in field assays,...
Tipo: Journal article Palavras-chave: Antifouling; Biofouling; Bioactive compounds; Marine biotechnology; Marine microbes.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300012
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SAR of Sponge-Inspired Hemibastadin Congeners Inhibiting Blue Mussel PhenolOxidase ArchiMer
Niemann, Hendrik; Hagenow, Jens; Chung, Mi-young; Hellio, Claire; Weber, Horst; Proksch, Peter.
Hemibastadin derivatives, including the synthetically-derived 5,5-dibromohemibastadin-1 (DBHB), are potent inhibitors of blue mussel phenoloxidase (PO), which is a key enzyme involved in the firm attachment of this invertebrate to substrates and, thus, a promising molecular target for anti-fouling research. For a systematic investigation of the enzyme inhibitory activity of hemibastadin derivatives, we have synthesized nine new congeners, which feature structural variations of the DBHB core structure. These structural modifications include, e.g., different halogen substituents present at the aromatic rings, different amine moieties linked to the (E)-2-(hydroxyimino)-3-(4-hydroxyphenyl)propionic acid, the presence of free vs. substituted aromatic hydroxyl...
Tipo: Text Palavras-chave: Antifouling; Hemibastadin; Phenoloxidase; Sponges; Copper; Mytilus edulis.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00273/38432/73167.pdf
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Screening of marine algal extracts for anti-settlement activities against microalgae and macroalgae ArchiMer
Hellio, Claire; Berge, Jean-pascal; Beaupoil, Claude; Le Gal, Yves; Bourgougnon, Nathalie.
The ban on the use of TBT-based antifouling paints for boats under 25 m in length has lead to a search for new non-toxic antifoulants. One of the most promising alternative technologies to heavy metal based antifouling paint is the development of antifouling coatings whose active ingredients are naturally occurring compounds from marine organisms. This is based on the principle that marine organisms are also facing the problem of the presence of epibionts on their own surface. In this study, the antifouling activity of a series of aqueous, ethanolic and dichloromethane extracts from 30 algae from the North East Atlantic coast was investigated. The extracts were tested in laboratory assays against species representatives of two major groups of fouling...
Tipo: Text Palavras-chave: Antifouling; Antialgal; Microalgae; Macroalgae; Screening; Settlement; Spores.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/00057/16795/14304.pdf
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