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Actinobactérias como agentes de controle biológico de fitopatógenos de trigo. Repositório Alice
VENTURA, J. P.; CASTELIANI, A. G. B.; VARGAS HOYOS, H. A.; SANTOS, S. N.; MELO, I. S. de.
Resumo: O trigo (Triticum aestivum L) constitui uma das principais culturas de importância agrícola para o Brasil. Contudo, a ocorrência de doenças como a brusone, tem limitado a produtividade dessa cultura nas regiões produtoras. Desse modo, o objetivo desse trabalho foi avaliar o potencial de uma linhagem Streptomyces sp. CMAA 1647, uma actinobacteria, no controle biológico de fungos fitopatogênicos, principalmente, Pyricularia grisea, agente causal da brusone. A linhagem modelo deste trabalho, Streptomyces sp. CMAA 1647, foi isolada a partir de solos supressivos à brusone e previamente selecionada por inibir o crescimento do agente causal. O potencial de antagonismo da linhagem CMAA 1647 foi avaliado pela técnica de pareamento direto contra os seguintes...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Solos supressivos; Atividade antifúngica; Tratamento de sementes; Antifungal activity; Germination potential; Brusone; Doença de planta; Controle biológico; Trigo; Tratamento de semente; Streptomyces; Suppressive soils; Antifungal agents; Seed treatment; Suppressive soils.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105031
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Antifungal activity of bacterial strains from the rhizosphere of Stachytarpheta crassifolia. Repositório Alice
SANTOS, S. N.; OLIVEIRA, L. K. X.; MELO, I. S. de; VELOSO, E. da S.; ROQUE, M. R. de A..
This study evaluated the antifungal action of biomolecules produced from the secondary metabolism of bacterial strains found in the rhizosphere of semi arid plants against human pathogenic Candida albicans. Crude extracts were obtained using ethyl acetate as an organic solvent and the bioactivity was assessed with a bioautography technique. The results showed that bacterial strains, Alcaligenes faecalis MRbS12 and Bacillus cereus MRbS26, had compounds with antifungal bioactivity. The largest inhibition zones for both compounds were located on spots with Rf values below 0.500, indicating that the molecules possibly had polar characteristics. These results suggested that microorganisms found in the environment could have bioprospecting potential.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biomolecules; Bioautoghaphy; Antifungal activity; Alcaligenes faecalis; Bacillus cereus; Candida albicans; Bactéria; Rizosfera; Controle biológico; Rhizobiales; Antifungal agents; Biological control.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/898255
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Antimicrobial alumina nanobiostructures of disulfide- and triazole-linked peptides: Synthesis, characterization, membrane interactions and biological activity. Repositório Alice
TORRES, L. M. F. C.; ALMEIDA, M. T.; SANTOS, T. L.; MARINHO, L. E. S.; MESQUITA, J. P. de; SILVA, L. M. da; SANTOS, W. T. P. dos; MARTINS, H. R.; KATO, K. C.; ALVES, E. S. F.; LIAO, L. M.; MAGALHÃES, M. T. Q. de; MENDONÇA, F. G. de; PEREIRA, F. V.; RESENDE, J. M.; BEMQUERER, M. P.; RODRIGUES, M. A.; VERLY, R. M..
bitstream/item/197769/1/1-s2.0-S0927776519300529-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Nanobiomaterials; Peptide-decorated alumina nanoparticles; Alumina nanoparticles; Peptide synthesis; Antifungal activity; Antibacterial activity; Copper(I)-catalyzed cycloaddition reaction; Antimicrobial peptides.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109343
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Antimicrobial properties of two novel peptides derived fromTheobroma cacao osmotin. Repositório Alice
FALCAO, L. L.; SILVA WERNECK, J. O.; RAMOS, A. de R.; MARTINS, N. F.; BRESSO, E.; RODRIGUES, M. A.; BEMQUERER, M. P.; MARCELLINO, L. H..
bitstream/item/149152/1/Bemquerer-2016-Peptides-Antimicrobial-properties-of-two-novel-peptides-derived-from-Theobroma-cacao-osmotin.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Antimicrobial peptide; Osmotin-like protein; Pathogenesis-related; Antifungal activity; Phytopathogens; Yeast; Moniliophthora perniciosaa; Cação.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055262
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Changes in hyphal morphology due to chitosan treatment in some fungal species. Repositório Alice
OLIVEIRA JUNIOR, E. N.; MELO, I. S. de; FRANCO, T. T..
In this work, changes in the hyphal morphology due to chitosan treatment in some fungal species were studied. Scanning electron microscope (SEM) observations revealed that chitosans with molar fraction of acetyl groups (FA 0.16 and 0.18) and degree of polymerization (DP 1,089 and 1,242) had a direct effect on the morphology of the chitosan-treated fungi, reflecting its potential for causing a delay in the growth of Alternaria alternata (500 µg × mL-1), Botrytis cinerea (1,000 µg × mL-1), Penicillium expansum (1,000 µg × mL-1) and Rhizopus stolonifer (500 µg × mL-1). Mycelial aggregation and structural changes such as excessive branching, swelling of the cell wall and hyphae size reduction were observed in the micrographs.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Alternative control of fungi; Antifungal activity; Fungal morphology; Fungo; Doença fúngica; Chitosan; Antifungal agents; Plant pathogenic fungi.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/941619
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Characterization of the antifungal activity of Lactobacillus harbinensis K.V9.3.1Np and Lactobacillus rhamnosus K.C8.3.1I in yogurt ArchiMer
Delavenne, Emilie; Cliquet, Sophie; Trunet, Clément; Barbier, Georges; Mounier, Jérome; Le Blay, Gwenaelle.
Few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. Consumer’s demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. In this study, using a 25-1 factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contamination amount of a spoilage yeast, on antifungal activities of single and mixed cultures of Lactobacillus rhamnosus K.C8.3.1I and Lactobacillus harbinensis K.V9.3.1Np in yogurt. L. harbinensis K.V9.3.1Np, the most relevant strain with regard to antifungal activity was...
Tipo: Text Palavras-chave: Lactobacillus harbinensis; Bioprotective culture; Antifungal activity.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00189/30025/28510.pdf
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In vitro effect of essential oils on Alternaria solani Sorauer Rev. Protección Veg.
Duarte,Yanisia; Pino,Oriela; Infante,Danay; Sánchez,Yaima; Travieso,María del Carmen; Martínez,B.
The aim of this work was to determine the in vitro antifungal activity of ten essential oils on Alternaria solani SORAUER. The effect by direct contact and exposure to vapors was evaluated. The bioassays were performed using a completely randomized design and the method of paper discs inoculated with the oils facing phytopathogen discs; the fungal radial growth was evaluated. All the oils, except those from Citrus sinensis (L.) Osbeck (sweet orange), inhibited the mycelial growth until the seventh day. At day 14, a total inhibition was observed in the treatments with the oils from Pimpinella anisum L. (anise), Ocimum basilicum L. (white basil), Ocinum basilicum L. var. genovese (Genovese basil), and Piper auritum Kunth (anise caisimon). No fungicidal...
Tipo: Journal article Palavras-chave: Essential oils; Antifungal activity; Ocimum basilicum; Piper marginatum; Ruta chalepensis; Pimpinella anisum; Piper auritum; Piper aduncum subsp. Ossanum; Citrus sinensis.
Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522013000100007
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Mycoparasitic nature of Bionectria sp. strain 6.21. Repositório Alice
MELO, I. S. de; VALENTE, A. M. M. P.; KAVAMURA, V. N.; VILELA, E. S. D.; FAULL, J. L..
Abstract: In this study, a Bionectria sp. strain isolated from citrus rhizosphere was evaluated for its potential in inhibiting the growth of Rhizoctonia solani and Pythium aphanidermatum. It was demonstrated that Bionectria sp. 6.21 inhibited the growth of P. aphanidermatum and R. solani. In dual cultures, however, the antagonist only parasitised R. solani. Regarding the assay involving P. aphanidermatum, a lack of mycoparasitic ability was demonstrated. Crude extract of Bionectria completely inhibited the mycelial growth of both fungi. It appears that the main mechanism involved in the antagonism of Pythium by Bionectria is through antibiotic production. The antagonistic fungus released extracellular secondary metabolites. The metabolites were found to...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Atividade antifúngica; Antifungal activity; Bionectria sp; Enzymes; Plant diseases; Fungo; Fungo para controle biológico; Controle biológico; Rhizoctonia solani; Pythium aphanidermatum; Doença de planta; Fungi; Plant diseases and disorders; Biological control agents; Antifungal properties; Mycoparasits.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1030877
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Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrancosa) leaves. IPB - Escola Superior Agrária
Pereira, A.P.; Ferreira, Isabel C.F.R.; Marcelino, F.; Valentão, P.; Andrade, P.B.; Seabra, R.M.; Estevinho, Leticia M.; Bento, Albino; Pereira, J.A..
We report the determination of phenolic compounds in olive leaves by reversedphase HPLC/DAD, and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely Gram positive (Bacillus cereus, B. subtilis and Staphylococcus aureus), Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae) and fungi (Candida albicans and Cryptococcus neoformans). Seven phenolic compounds were identified and quantified: caffeic acid, verbascoside, oleuropein, luteolin 7-O-glucoside, rutin, apigenin 7-O-glucoside and luteolin 4’-O-glucoside. At low concentrations olive leafs extracts showed an unusual combined antibacterial and antifungal action,...
Tipo: Article Palavras-chave: Olive leaves; Phenolic compounds; Antibacterial activity; Antifungal activity.
Ano: 2007 URL: http://hdl.handle.net/10198/742
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The antifungal effect of six commercial extracts of Chilean propolis on Candida spp Ciencia e Investigación Agraria
Herrera,Christian L; Alvear,Marysol; Barrientos,Leticia; Montenegro,Gloria; Salazar,Luis A.
Propolis has been used in traditional medicine for many centuries because of its benefcial health properties, including its antimicrobial capacity. Prosthesis stomatitis affects a signifcant percentage of users of removable dentures; Candida albicans is the most common fungal species associated with the development of this pathology. Thus, the objectives of this study were: a. To evaluate the antifungal activity of six commercial propolis extracts against Candida spp. that was isolated from the oral cavity of removable dentures users, and b. To determine chemical characteristics of the propolis extracts evaluated. Among the results, we note that these concentrations of polyphenols varied between 9 ± 0.3 and 85 ± 2.1 mg mL-1. Chromatographic analysis was...
Tipo: Journal article Palavras-chave: Antifungal activity; Candida albicans; Propolis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000100007
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The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance Ciencia e Investigación Agraria
Curifuta,Macarena; Vidal,Jorge; Sánchez-Venegas,Jaime; Contreras,Aliro; Salazar,Luis A; Alvear,Marysol.
Propolis has been used for millennia for its antimicrobial and pharmaceutical properties, whereas its use as an agricultural antifungal agent has only recently been assessed. The chemical characteristics of six ethanolic extracts of Chilean propolis (EEP-1 to EEP-6) were evaluated. The total polyphenols ranged between 7.8 and 42.3 mg mL-1 equivalents of a 2:1 pinocembrin: galangin standard, and the total flavonoid contents ranged between 4.0 and 19.7 mg mL-1 equivalents of quercetin. A high-performance liquid chromatographic (HPLC) analysis allowed the identification of caffeic acid, myricetin, quercetin, kaempferol, apigenin, pinocembrin, galangin, caffeic acid phenethyl ester (CAPE) and rutin. The EEP-3 extract was selected for additional studies based...
Tipo: Journal article Palavras-chave: Alternaria alternata; Antifungal activity; Botrytis cinerea; Flavonoids; Fusarium sp.; Penicillium expansum; Polyphenols; Propolis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200011
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The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein Electron. J. Biotechnol.
Wang,Mengqiang; Zhang,Daoxiang; Wang,Lingling; Gai,Yunchao; Zhou,Zhi; Zhang,Huan; Song,Linsheng.
Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities. Results: The full-length cDNA of crab Eriocheir sinensis CypA (EsCypA) was cloned by EST and RACE technique. The complete sequence of EsCypA cDNA contained a 5' untranslated region (UTR) of 50 bp, a 3’ UTR of 233 bp with a polyA tail, and an open reading frame (ORF) of 495 bp encoding a polypeptide of 164 amino acids with the predicted molecular weight of 17.36 kDa. The deduced amino acid sequence of EsCypA contained two highly conserved signature sequences of...
Tipo: Journal article Palavras-chave: Antifungal activity; Cyclophilin A; Eriocheir sinensis; Immune response; PPIase activity.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200002
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