Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 18
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
A hemocyanin-derived antimicrobial peptide from the penaeid shrimp adopts an alpha-helical structure that specifically permeabilizes fungal membranes ArchiMer
Petit, Vanessa W.; Rolland, Jean-luc; Blond, Alain; Cazevieille, Chantal; Djediat, Chakib; Peduzzi, Jean; Goulard, Christophe; Bachere, Evelyne; Dupont, Joelle; Destoumieux-garzon, Delphine; Rebuffat, Sylvie.
Background. Hemocyanins are respiratory proteins with multiple functions. In diverse crustaceans hemocyanins can release histidine-rich antimicrobial peptides in response to microbial challenge. In penaeid shrimp, strictly antifungal peptides are released from the C-terminus of hemocyanins. Methods. The three-dimensional structure of the antifungal peptide PvHCt from Litopenaeus vannamei was determined by NMR. Its mechanism of action against the shrimp pathogen Fusarium oxysporum was investigated using immunochemistry, fluorescence and transmission electron microscopy. Results. PvHCt folded into an amphipathic α-helix in membrane-mimicking media and displayed a random conformation in aqueous environment. In contact with F. oxysporum, PvHCt bound...
Tipo: Text Palavras-chave: Antimicrobial peptide; Amphipathic helix; Fungi; Membrane bilayer; Nuclear magnetic resonance (NMR); Fluorescence microscopy.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00301/41202/40362.pdf
Imagem não selecionada

Imprime registro no formato completo
Antimicrobial properties of two novel peptides derived fromTheobroma cacao osmotin. Repositório Alice
FALCAO, L. L.; SILVA WERNECK, J. O.; RAMOS, A. de R.; MARTINS, N. F.; BRESSO, E.; RODRIGUES, M. A.; BEMQUERER, M. P.; MARCELLINO, L. H..
bitstream/item/149152/1/Bemquerer-2016-Peptides-Antimicrobial-properties-of-two-novel-peptides-derived-from-Theobroma-cacao-osmotin.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Antimicrobial peptide; Osmotin-like protein; Pathogenesis-related; Antifungal activity; Phytopathogens; Yeast; Moniliophthora perniciosaa; Cação.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055262
Imagem não selecionada

Imprime registro no formato completo
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas ArchiMer
Rosa, Rafael D.; Alonso, Pascal; Santini, Adrien; Vergnes, Agnes; Bachere, Evelyne.
We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in...
Tipo: Text Palavras-chave: Mollusk bivalve; Antimicrobial peptide; Polymorphism; Gene copy number variation; High-throughput microfluidic RT-qPCR; Vibrio infection.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00239/35009/33545.pdf
Imagem não selecionada

Imprime registro no formato completo
Influence of temperature, salinity and E. coli tissue content on immune gene expression in mussel: Results from a 2005-2008 survey ArchiMer
Li, Hui; Toubiana, Mylene; Monfort, Patrick; Roch, Philippe.
Several bivalves, including mussels, suffered from mortalities particularly in summer. To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-PCR was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and HSP70. House keeping gene was 28S rRNA. Defensin, myticin B and lysozyme appeared more expressed in spring-summer than in winter. In contrast, HSP70 expression was higher in winter. Statistical studies using principal component analysis (PCA) and multiple regression models revealed positive influence of temperature on...
Tipo: Text Palavras-chave: Mollusks; Mytilus; Innate immunity; Seasonal variations; Immune survey; Q PCR; HSP70; Lysozyme; Myticin; Mytilin; Defensin; Antimicrobial peptide.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7345.pdf
Imagem não selecionada

Imprime registro no formato completo
Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding Electron. J. Biotechnol.
Almasia,Natalia I; Narhirñak,Vanesa; Hopp,H. Esteban; Vazquez-Rovere,Cecilia.
Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons,...
Tipo: Journal article Palavras-chave: Antimicrobial peptide; Inducible promoter; Potato; Snakin/GASA.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500008
Imagem não selecionada

Imprime registro no formato completo
Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infections ArchiMer
Farias, Natanael Dantas; Falchetti, Marcelo; Matos, Gabriel Machado; Schmitt, Paulina; Barreto, Cairé; Argenta, Nicolas; Rolland, Jean-luc; Bachère, Evelyne; Perazzolo, Luciane Maria; Rosa, Rafael Diego.
Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. vannamei stylicin family is composed of two members (Lvan-Stylicin1 and Lvan-Stylicin2) encoded by different loci which vary in gene copy number. Unlike the other three gene-encoded antimicrobial peptide families from penaeid shrimp, the expression of Lvan-Stylicins is not restricted to hemocytes. Indeed, they are also produced by the columnar epithelial cells lining the...
Tipo: Text Palavras-chave: Crustacean; Penaeid shrimp; Invertebrate immunity; Antimicrobial peptide; Host defense peptide; Molecular diversity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00466/57816/60102.pdf
Imagem não selecionada

Imprime registro no formato completo
LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha. Repositório Alice
SANTOS, D. M.; VERLY, R. M.; PILÓ VELOSO, D.; MARIA, M. de; CARVALHO, M. A. R. de; CISALPINO, P. S.; SOARES, B. M.; DINIZ, C. G.; FARIAS, L. M.; MOREIRA, D. F. F.; FRÉZARD, F.; BEMQUERER, M. P.; PIMENTA, A. M. C.; LIMA, M. E. de.
bitstream/item/181851/1/Santos2010-Article-LyeTxIAPotentAntimicrobialPept.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Lycosa erythrognatha; Antimicrobial peptide; LyeTx I; Spider venom.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/871814
Imagem não selecionada

Imprime registro no formato completo
Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp ArchiMer
Matos, Gabriel Machado; Schmitt, Paulina; Barreto, Caire; Farias, Natanael Dantas; Toledo-silva, Guilherme; Guzman, Fanny; Destoumieux-garzon, Delphine; Perazzolo, Luciane Maria; Rosa, Rafael Diego.
Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a central β-hairpin structure able to bind to microbial components. Mining sequence databases for ALFs allowed us to show the remarkable diversity of ALF sequences in shrimp. We found at least seven members of the ALF family (Groups A to G), including two novel Groups (F and G), all of which are encoded by different loci with conserved gene organization. Phylogenetic analyses revealed that gene expansion and subsequent diversification of the ALF family occurred in crustaceans before shrimp speciation occurred. The transcriptional profile of ALFs was compared in terms of tissue distribution, response to two pathogens and during shrimp development in Litopenaeus vannamei, the most...
Tipo: Text Palavras-chave: Host defense peptide; Antimicrobial peptide; Anti-LPS factor; Hostmicrobe relationship; Functional diversity; Invertebrate immunity; Crustacean; Antimicrobial activity.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00464/57523/59711.pdf
Imagem não selecionada

Imprime registro no formato completo
Mode de reconnaissance hôte symbionte en milieux extrêmes : cas du modèle symbiotique Rimicaris exoculata ArchiMer
Le Bloa, Simon.
Deprived of light, the deep-sea hydrothermal vents are extremes ecosystems sustained by micobial chemosynthesis. These environments are colonized by animal species living in close relationships with these chemoautotrophic micro-organisms, eating them or establishing long term interactions with them, may they be trophic or not only. The shrimp Rimicaris exoculata is an endemic hydrothermal species of the Mid-Atlantic Ridge (MAR) sites. This crustacean represents the predominant macrofauna of some sites of the MAR. It lives in symbiotic association with two distinct microbial communities qualified as ectosymbiosis. One is located in its gill chamber and one in its gut. First, this work focused on the study of bacterial communication (Quorum Sensing or QS)...
Tipo: Text Palavras-chave: Écosystèmes hydrothermaux; Peptide antimicrobien; Marqueurs génétiques; Hydrothermal vent; Rimicaris exoculata; Symbiose; Quorum Sensing; Antimicrobial peptide; Genetic marker.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00498/60918/64279.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular characterization of two isoforms of defensin from hemocytes of the oyster Crassostrea gigas ArchiMer
Gonzalez, Marcelo; Gueguen, Yannick; Desserre, Geraldine; De Lorgeril, Julien; Romestand, Bernard; Bachere, Evelyne.
Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. We previously characterized the first AMP from an oyster, a defensin, that was shown to be continuously expressed in the mantle of Crassostrea gigas. In this study, we report the cDNA cloning of two new isoforms of the defensin AMP family (Cg-defh1 and Cg-defh2) from the hemocytes of the oyster. The deduced amino acid sequences reveal two peptides of 73 amino acid residues with a mature portion consisting of 43 amino acid residues. Cy-Defh1 and CyDefh2 share 86% amino acid identity and belong to the "arthropod-molluscs defensin family". qRT-PCR analyses indicate that Cg-defh2 is continuously expressed in the hemocytes of C. gigas. In...
Tipo: Text Palavras-chave: Defensin; Antimicrobial peptide; Hemocytes; Pacific oyster; Marine invertebrate; Mollusc bivalve.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2331.pdf
Imagem não selecionada

Imprime registro no formato completo
NMR structure of mussel mytilin, and antiviral-antibacterial activities of derived synthetic peptides ArchiMer
Roch, Philippe; Yang, Y; Toubiana, Mylene; Aumelas, A.
Mytilin is a 34-residue antibacterial peptide from the mussel Mytilus galloprovincialis, which in addition possesses in vitro antiviral activity. The three-dimensional solution structure of the synthetic mytilin was established by using 1H NMR and consists of the common cysteine-stabilized alpha beta motif close to the one observed in the mussel defensin MGD-1. Mytilin is characterized by 8 cysteines engaged in four disulfide bonds (2-27, 6-29, 10-31, and 15-34) only involving the beta-strand II. Hydrophilic and hydrophobic areas of mytilin account for 63% and 37%, respectively, a ratio very close to that of MGD-1 (64% and 36%). One linear and three cyclic fragments were designed from the interstrand loop sequence known to retain the biological activities...
Tipo: Text Palavras-chave: Shrimp; Molluscs; Mussel; Antibacterial activity; WSSV; Viral protection; Beta hairpin structure; NMR; Mytilin; Antimicrobial peptide.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4485.pdf
Imagem não selecionada

Imprime registro no formato completo
Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin ArchiMer
Gueguen, Yannick; Romestand, Bernard; Fievet, Julie; Schmitt, Paulina; Destoumieux Garzon, Delphine; Franck, Vandenbulcke; Philippe, Bulet; Bachere, Evelyne.
A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an...
Tipo: Text Palavras-chave: Antimicrobial peptide; Mollusk; Synergy; Invertebrate; Innate immunity; Pacific oyster.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6232.pdf
Imagem não selecionada

Imprime registro no formato completo
Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge ArchiMer
Destoumieux, Delphine; Munoz, Marcello; Cosseau, Céline; Rodriguez, Jenny; Bulet, Philippe; Comps, Marie-annick; Bachere, Evelyne.
Penaeidins are members of a new family of antimicrobial peptides isolated from a crustacean, which present both Gram-positive antibacterial and antifungal activities. We have studied the localization of synthesis and storage of penaeidins in the shrimp Penaeus vannamei. The distribution of penaeidin transcripts and peptides in various tissues reveals that penaeidins are constitutively synthesized and stored in the shrimp haemocytes. It was shown by immunocytochemistry, at both optical and ultrastructural levels, that the peptides are localized in granulocyte cytoplasmic granules, The expression and localization of penaeidins were further analysed in shrimp subjected to microbial challenge. We found that (1) penaeidin mRNA levels decrease in circulating...
Tipo: Text Palavras-chave: Crustacean immunity; Penaeid shrimp; Haemocyte; Chitin binding; Antimicrobial peptide.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-412.pdf
Imagem não selecionada

Imprime registro no formato completo
PenBase, the shrimp antimicrobial peptide penaeidin database: Sequence-based classification and recommended nomenclature ArchiMer
Gueguen, Yannick; Garnier, Julien; Robert, Lorenne; Lefranc, Marie-paule; Mougenot, Isabelle; De Lorgeril, Julien; Janech, Michael; Gross, Paul S.; Warr, Gregory W.; Cuthbertson, Brandon; Barracco, Margherita A.; Bulet, Philippe; Aumelas, André; Yang, Yinshan; Bo, Dong; Xiang, Jianhai; Tassanakajon, Anchalee; Piquemal, Piquemal; Bachere, Evelyne.
Antimicrobial peptides play a major role in innate immunity. The penaeidins, initially characterized from the shrimp Litopenaeus vannamei, are a family of antimicrobial peptides that appear to be expressed in all penaeid shrimps. As of recent, a large number of penaeid nucleotide sequences have been identified from a variety of penaeid shrimp species and these sequences currently reside in several databases under unique identifiers with no nomenclatural continuity. To facilitate research in this field and avoid potential confusion due to a diverse number of nomenclatural designations, we have made a systematic effort to collect, analyse, and classify all the penaeidin sequences available in every database. We have identified a common penaeidin signature...
Tipo: Text Palavras-chave: Nomenclature; Sequence database; Crustacea; Antimicrobial peptide; Penaeid shrimp; Penaeidin.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2332.pdf
Imagem não selecionada

Imprime registro no formato completo
Peptide: lipid ratio and membrane surface charge determine the mechanismof action of the antimicrobial peptide BP100. Conformational and functional studies. Repositório Alice
MANZINI, M. C.; PEREZ, K. R.; RISKE, K. A.; BOZELLI JUNIOR, J. C.; SANTOS, T. L.; SILVA, M. A. da; SARAIVA, G. K. V.; POLITI, M. J.; VALENTE, A. P.; ALMEIDA, F. C. L.; CHAIMOVICH, H.; RODRIGUES, M. A.; BEMQUERER, M. P.; SCHREIER, S.; CUCCOVIA, I. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: BP100; Antimicrobial peptide; CD; NMR; Zeta potential; Model membrane leakage.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1005876
Imagem não selecionada

Imprime registro no formato completo
The impact of insecticide resistance on Culex pipiens immunity ArchiMer
Vezilier, Julien; Nicot, Antoine; De Lorgeril, Julien; Gandon, Sylvain; Rivero, Ana.
Because of their role as vectors of diseases, the evolution of insecticide resistance in mosquitoes has been intensively investigated. Insecticide resistance is associated to a wide range of pleiotropic effects on several key life-history traits of mosquitoes such as longevity and behavior. However, despite its potential implications in pathogen transmission, the effects of insecticide resistance on mosquito immunity have received little, if any, attention. Here, we investigate the impact of insecticide resistance in Culex pipiens, an epidemiologically important vector of a wide array of pathogens. Using both isogenic laboratory strains and field-caught mosquitoes, we investigate the impact of two main insecticide resistance mechanisms (metabolic...
Tipo: Text Palavras-chave: Acetylcholinesterase; Anopheles; Antimicrobial peptide; Carboxylesterases; Nitric oxide; Transferrin.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00136/24773/22845.pdf
Imagem não selecionada

Imprime registro no formato completo
The new insights into the oyster antimicrobial defense: Cellular, molecular and genetic view ArchiMer
Bachere, Evelyne; Rosa, Rafael Diego; Schmitt, Paulina; Poirier, Aurore; Merou, Nicolas; Charriere, Guillaume; Destoumieux-garzon, Delphine.
Oysters are sessile filter feeders that live in close association with abundant and diverse communities of microorganisms that form the oyster microbiota. In such an association, cellular and molecular mechanisms have evolved to maintain oyster homeostasis upon stressful conditions including infection and changing environments. We give here cellular and molecular insights into the Crassostrea gigas antimicrobial defense system with focus on antimicrobial peptides and proteins (AMPs). This review highlights the central role of the hemocytes in the modulation and control of oyster antimicrobial response. As vehicles for AMPs and other antimicrobial effectors, including reactive oxygen species (ROS), and together with epithelia, hemocytes provide the oyster...
Tipo: Text Palavras-chave: Mollusk; Immunity; Antimicrobial peptide; Hemocyte; Defensin.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00254/36552/35097.pdf
Imagem não selecionada

Imprime registro no formato completo
Toxicity evaluation to mice of Phylloseptin-1, an antimicrobial peptide from the skin secretion of Phyllomedusa hypochondrialis (Amphibia). Repositório Alice
KÜCKELHAUS, S.; LEITE, J. R. S. A.; NEVES, M. P.; FROTA, K. S.; ABDALA, L. F.; JUNQUEIRA, M. I. M.; BLOCH JUNIOR, C.; TOSTA, C. E..
bitstream/item/178132/1/ID-27936-1.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Phylloseptin-1; Antimicrobial peptide; Phyllomedusa hypochondrialis; Mouse; Antimicróbico; Peptídeo; Rato; Cytotoxicity; Genotoxicity.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/187694
Registros recuperados: 18
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional