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Registros recuperados: 29
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Antifungal activity of bacterial strains from the rhizosphere of Stachytarpheta crassifolia. Repositório Alice
SANTOS, S. N.; OLIVEIRA, L. K. X.; MELO, I. S. de; VELOSO, E. da S.; ROQUE, M. R. de A..
This study evaluated the antifungal action of biomolecules produced from the secondary metabolism of bacterial strains found in the rhizosphere of semi arid plants against human pathogenic Candida albicans. Crude extracts were obtained using ethyl acetate as an organic solvent and the bioactivity was assessed with a bioautography technique. The results showed that bacterial strains, Alcaligenes faecalis MRbS12 and Bacillus cereus MRbS26, had compounds with antifungal bioactivity. The largest inhibition zones for both compounds were located on spots with Rf values below 0.500, indicating that the molecules possibly had polar characteristics. These results suggested that microorganisms found in the environment could have bioprospecting potential.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biomolecules; Bioautoghaphy; Antifungal activity; Alcaligenes faecalis; Bacillus cereus; Candida albicans; Bactéria; Rizosfera; Controle biológico; Rhizobiales; Antifungal agents; Biological control.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/898255
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Asn336 is involved in the substrate affinity of glycine oxidase from Bacillus cereus Electron. J. Biotechnol.
Wu,Gaobing; Zhan,Tao; Guo,Yiming; Kumar,Ashok; Liu,Ziduo.
Background: Glycine oxidase (GO), a type of D-amino acid oxidase, is of biotechnological interest for its potential in several fields. In our previous study, we have characterized a new glycine oxidase (BceGO) from Bacillus cereus HYC-7. Here, a variant of N336K with increased the affinity against all the tested substrate was obtained by screening a random mutant library of BceGO. It is observed that the residue N336 is invariable between its homogeneous enzymes. This work was aimed to explore the role of the residue N336 in glycine oxidase by site-directed mutagenesis, kinetic assay, structure modeling and substrate docking. Results: The results showed that the affinity of N336H, N336K and N336R increased gradually toward all the substrates, with increase...
Tipo: Journal article Palavras-chave: Bacillus cereus; Error-prone PCR; Glycine oxidase; Site-directed mutagenesis; Substrate affinity.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400004
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Assessment of hydrophobicity and roughness of stainless steel adhered by an isolate of Bacillus cereus from a dairy plant BJM
Bernardes,Patrícia Campos; Andrade,Nélio José de; Ferreira,Sukarno Olavo; Sá,João Paulo Natalino de; Araújo,Emiliane Andrade; Delatorre,Deyse Maria Zanom; Luiz,Lívia Maria Pinheiro.
The interaction between the surface of stainless steel and Bacillus cereus was studied in terms of the characteristics of interfacial interaction determined from the measurement of the contact angle of the surface of B. cereus and stainless steel in the presence or absence of B. cereus adherence. The microtopographies and the roughness of the surface of stainless steel and stainless steel adhered by B. cereus were evaluated with the help of atomic force microscopy and perfilometry. The strain of B. cereus studied was considered hydrophilic, whereas the stainless steel was considered hydrophobic. The adhesion was not thermodynamically favorable (ΔGadhesion > 0) between the stainless steel and the strain of B. cereus studied. Thus, the interaction between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrophobicity; Roughness; Bacillus cereus; Dairy plant; Adhesion.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400017
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Beneficial effects of bio-controlling agent Bacillus cereus IB311 on the agricultural crop production and its biomass optimization through response surface methodology Anais da ABC (AABC)
BANERJEE,GOUTAM; GORTHI,SRIKANTH; CHATTOPADHYAY,PRITAM.
ABSTRACT Disease in agricultural field is a big problem that causes a massive loss in production. In this present investigation, we have reported a soil-borne bacterium Bacillus cereus IB311 which is antagonistic to plant pathogens (Pseudomonas syringae and Agrobacterium tumefaciens), and could make a substantial contribution to the prevention of plant diseases. To prove the practical application, the strain was directly applied in agricultural field. The results demonstrated that B. cereus IB311 has increased the production (20% and 26% in term of average pod number per plant, average seed number per pod, and seed yield per experimental plot) in ground nut (Arachis hypogaea var. Koushal, G201) and sesame (Sesamum indicum var. Kanak), respectively. To...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Bio-control; Phytopathogens; RSM; Field application; Crop production.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502149
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Biocontrole da mancha-angular do feijoeiro por antagonistas bacterianos PAB
Garcia,Flávio Augusto de Oliveira; Romeiro,Reginaldo da Silva.
O objetivo deste trabalho foi avaliar o efeito de isolados de Bacillus cereus e Pseudomonas putida sobre a severidade da mancha-angular do feijoeiro, em condições de campo. Quatro experimentos foram conduzidos em épocas favoráveis à mancha-angular (Pseudocercospora griseola), com as cultivares Pérola e Ouro Negro. Os experimentos foram realizados em delineamento de blocos ao acaso, com seis repetições. Os tratamentos foram pulverizações sobre as plantas com três estirpes de B. cereus (UFV-172, UFV-101 e UFV-75) e duas estirpes de P. putida (UFV-53 e UFV-Pp), acibenzolar-S-metil, fungicida clorotalonil e controle pulverizado com água, no total de oito tratamentos. Os tratamentos foram aplicados por atomização no filoplano de plantas de feijoeiro,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Phaseolus vulgaris; Pseudocercospora griseola; Pseudomonas putida.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001200004
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Biological control of Mycosphaerella fragariae in strawberry culture Sci. Agrar.Paran. / SAP
Heling, Anderson Luis; Kuhn, Odair José; Stangarlin, José Renato.
The Mycosphaerella spot is one of the main foliar diseases of strawberry, degrating great leaf regions and reducing the photosynthetic area. Its control is mainly by the use of chemical fungicides, but, due the increasing demand for food free of pesticide, alternative control methods have been researched, such as biological control. This work aimed to evaluate the effect on strawberry plants, treated with the biological control agents Bacillus cereus, Saccharomyces boulardii and Saccharomyces cerevisiae, in the severity of Mycosphaerella fragariae, productivity and in the activity of β-1.3 glucanases, peroxidases and chitinases enzymes. It was verified that S. cerevisiae and B. cereus treatments were similar to fungicide for disease control. However, even...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Controle alternativo; Bacillus cereus; Saccharomyces boulardii; Saccharomyces cerevisiae.
Ano: 2015 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/9544
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Caracterización in vitro de bacterias ácido lácticas con potencial probiótico Rev Salud Anim.
Sánchez,Lilian; Tromps,Jeannette.
Las propiedades beneficiosas de las bacterias ácido lácticas (BAL) posibilitan su uso como probióticas en animales y humanos. El objetivo del presente trabajo fue caracterizar, mediante pruebas in vitro, cepas BAL procedentes de fuente animal para considerar su posterior aplicación como probiótico en animales. Se evaluaron 72 cepas, identificadas hasta el nivel de género, de Lactobacillus spp., Leuconostoc spp., Lactococcus spp., Streptococcus spp. y Pediococcus spp. A todas las cepas se les determinó su estabilidad de crecimiento a diferentes pH, temperatura y tolerancia a altas concentraciones de cloruro de sodio. De ellas se seleccionaron 14 cepas que presentaron las mejores características como candidatos a probióticas por sus resultados a las pruebas...
Tipo: Journal article Palavras-chave: Bacterias ácido-láctica; Probióticos; Bacillus cereus; Staphylococcus aureus; Salmonella spp.; Pseudomonas aeruginosa.
Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200008
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Controle biológico da podridão radicular e promoção de crescimento em pepino hidropônico com microrganismos de manguezais. Repositório Alice
CORREA, E. B.; GALVAO, J. A. H.; BETTIOL, W..
O objetivo deste trabalho foi avaliar a capacidade de microrganismos de manguezais para controlar a podridão radicular causada por Pythium aphanidermatum e para promover o crescimento em pepino hidropônico (Cucumis sativus). Avaliaram-se 19 microrganismos quanto ao controle da doença em mini-hidroponia. Os microrganismos mais promissores para esse fim - Gordonia rubripertincta SO-3B-2 e a mistura dos isolados G. rubripertincta SO-3B-2, MB-P3A-49, MB-P3-C68 e SO-3L-3, de Pseudomonas stutzeri, e Bacillus cereus AVIC-3-6 - foram, posteriormente, testados quanto à promoção de crescimento do pepineiro, em casa de vegetação. Microrganismos de manguezais podem ter importância funcional no controle biológico da podridão radicular causada por P. aphanidermatum e na...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bacillus cereus; Gordonia rubripertincta; Pseudomonas stutzeri; Bioprospecção; Pepino; Cultivo hidropônico; Podridão radicular; Controle biológico; Pythium aphanidermatum; Mangue; Microrganismo; Cucumis sativus; Root rot; Biological control.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/898155
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Controle biológico da podridão radicular e promoção de crescimento em pepino hidropônico com microrganismos de manguezais PAB
Corrêa,Élida Barbosa; Galvão,José Abrahão Haddad; Bettiol,Wagner.
O objetivo deste trabalho foi avaliar a capacidade de microrganismos de manguezais para controlar a podridão radicular causada por Pythium aphanidermatum e para promover o crescimento em pepino hidropônico (Cucumis sativus). Avaliaram-se 19 microrganismos quanto ao controle da doença em mini-hidroponia. Os microrganismos mais promissores para esse fim - Gordonia rubripertincta SO-3B-2 e a mistura dos isolados G. rubripertincta SO-3B-2, MB-P3A-49, MB-P3-C68 e SO-3L-3, de Pseudomonas stutzeri, e Bacillus cereus AVIC-3-6 - foram, posteriormente, testados quanto à promoção de crescimento do pepineiro, em casa de vegetação. Microrganismos de manguezais podem ter importância funcional no controle biológico da podridão radicular causada por P. aphanidermatum e na...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Cucumis sativus; Gordonia rubripertincta; Pseudomonas stutzeri; Pythium aphanidermatum; Bioprospecção.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000200003
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Controlling Bacillus cereus adherence to stainless steel with different cleaning and sanitizing procedures used in dairy plants Arq. Bras. Med. Vet. Zootec.
Salustiano,V.C; Andrade,N.J; Ribeiro Junior,J.I; Fernandes,P.E; Lopes,J.P; Bernardes,P.C; Portugal,J.G.
Bacillus cereus adherence to stainless steel used to milk contact surfaces was observed, depending on the cleaning and sanitizing procedures applied and the physicochemical properties of the surfaces. Numbers of surviving B. cereus after hygiene procedures were affected by temperature, the concentrations of both alkaline and acid washes, and the pH of the chlorine solution. The adhesion of B. cereus to the stainless steel was not thermodynamically favorable, and the adherence of this microorganism occurred in lower number, in accordance to the thermodynamic aspect of adhesion.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Equipment surface; Dairy industry; Hygiene procedures.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600026
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Detection of Bacillus cereus isolated during ultra high temperature milk production flowchart through random amplified polymorphic DNA polymerase chain reaction Ciência Rural
Vidal,Ana Maria Centola; Rossi Junior,Oswaldo Durival; Abreu,Irlan Leite de; Bürger,Karina Paes; Cardoso,Marita Vedovelli; Gonçalves,Ana Carolina Siqueira; Rossi,Gabriel Augusto Marques; D'Abreu,Léa Furlan.
ABSTRACT: The present study focused on isolation Bacillus cereus during the UHT milk production and shelf life, to assess the enterotoxigenic production capacity of isolates and to evaluate the use of the RAPD-PCR technique to verify whether Bacillus cereus isolated at different phases of UHT milk processing belongs to the same strain. For this, six groups of milk samples composed of raw, pasteurized and UHT milk were collected from a processing plant. The results revealed that bacteria belonging to the Bacillus cereus group were isolated from 51.6%, 81.6% and from 13.8% of raw, pasteurized and UHT milk samples, respectively. About 50.0% of isolates from raw milk, 19.2% isolates from pasteurized milk and 70.7% isolates from UHT milk were capable of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Enterotoxins; Microbiological quality; Pasteurized milk; Raw milk; UHT milk.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200286
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Detection of enterotoxins produced by B. cereus through PCR analysis of ground and roasted coffee samples in Rio de Janeiro, Brazil Ciênc. Tecnol. Aliment.
Souza,Cyllene de Matos Ornelas da Cunha Corrêa de; Abrantes,Shirley de Mello Pereira.
Coffee is one of the most appreciated drinks in the world. Coffee ground is obtained from the fruit of a small plant that belongs to the genus Coffea. Coffea arabica and Coffea canephora robusta are the two most commercially important species. They are more commonly known as arabica and robusta, respectively. Two-thirds of Coffea arabica plants are grown in South and Central America, and Eastern Africa - the place of origin for this coffee species. Contamination by microorganisms has been a major matter affecting coffee quality in Brazil, mainly due to the harvesting method adopted. Brazilian harvests are based on fruits collected from the ground mixed with those that fall on collection cloths. As the Bacillus cereus bacterium frequently uses the soil as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; NHE; HBL; Coffee.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200025
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Draft genome sequence of plant growth-promoting drought-tolerant Bacillus sp. strain CMAA 1363 isolated from the Brazilian Caatinga biome. Repositório Alice
KAVAMURA, V. N.; SANTOS, S. N.; TAKETANI, R. G.; VASCONCELLOS, R. L. F.; MELO, I. S. de.
The strain of Bacillus sp. CMAA 1363 was isolated from the Brazilian Caatinga biome and showed plant growth-promoting traits and ability to promote maize growth under drought stress. Sequencing revealed genes involved in stress response and plant growth promotion. These genomic features might aid in the protection of plants against the negative effects imposed by drought.
Tipo: Nota Técnica/Nota Científica (ALICE) Palavras-chave: Growth promotion; Bacillus cereus; Estimulante de crescimento vegetal; Bacilo; Cacto.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1073617
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Enhancement of biohydrogen production from starch processing wastewater and further inside its ecosystem disclosed by 16S rDNA sequencing and FISH BABT
Sutthipattanasomboon,Chonlapin; Wongthanate,Jaruwan.
ABSTRACT Biohydrogen production from starch processing wastewater in this study resulted the highest yield of 61.75 mL H2/g COD at initial pH 7.0, thermophilic temperature, and iron concentration 800 mg Fe/L. The yield was 2-folded higher than the operation at mesophilic temperature or without iron addition. Cell immobilization by addition of biomaterials (BM) could improve the hydrogen yield by 2-folded comparing to the non-addition. BM from plants (loofa sponge) was found producing higher yield than that from animals (silk cocoon), and optimal concentration of BM was 5% (V/V). Furthermore, it was revealed further inside its ecosystem using SEM, 16S rDNA sequencing and FISH. There was found rod-shaped microorganisms of Bacillus cereus, which reported as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biohydrogen production; Starch processing wastewater; Biological supporting materials; Bacillus cereus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100600
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Frequência de Bacillus spp. em solos de diferentes sistemas de cultivo de arroz irrigado em Cachoeirinha, RS Bragantia
Fritz,Leila Lucia; Berlitz,Diouneia Lisiane; Macedo,Vera Regina Mussoi; Machado,Vilmar; Fiuza,Lidia Mariana.
Bacilos entomopatogênicos são bactérias observadas principalmente no solo, consideradas importantes agentes no controle biológico de doenças e pragas da agricultura. No entanto, existem poucos estudos que relatam a influência das práticas agrícolas nas populações de Bacillus spp. Essa pesquisa objetivou avaliar a freqüência de bactérias entomopatogênicas pertencentes ao gênero Bacillus obtidas de amostras de solo coletadas no ano agrícola de 2006/2007 de diferentes sistemas de cultivo de arroz irrigado da EEA-IRGA, Cachoeirinha (RS). Foram analisadas 36 amostras de solos, oriundas de três sistemas de plantio de arroz irrigado, pré-germinado (SPG), convencional (SPC), plantio direto (SPD) e de uma área sem plantio de arroz (C). Para tanto, um grama de solo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroecossistemas; Bacilos entomopatogênicos; Sistemas de plantio; Bacillus thuringiensis; Bacillus sphaericus; Bacillus cereus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200020
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Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil BJM
Reis,Andre L.S.; Montanhini,Maike T.M.; Bittencourt,Juliana V.M.; Destro,Maria T.; Bersot,Luciano S..
Bacillus cereusis an ubiquitous, spore-forming bacteria that can survive pasteurization and the majority of the heating processes used in the dairy industry. Besides, it is a pathogen responsible for different types of food poisoning. One type of foodborne disease caused by B.cereusis the diarrheal syndrome, which is caused by the ingestion of vegetative cells producing toxins in the small intestine. One virulence factor for the diarrheal syndrome is the toxin hemolysin BL (HBL), a three-component protein formed by the L1, L2 and B components. In order to evaluate the presence of diarrheal strains isolated from milk and dairy products, 63 B. cereus isolates were obtained from 260 samples of UHT milk, pasteurized milk and powdered milk, sold in commercial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Dairy products; Bacillus cereus; Pathogenicity; Detection.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400024
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Growth of enterotoxin producing Bacillus cereus in meat substrate at 10ºC and 30ºC BJM
Soares,Celina Mara; Kabuki,Dirce Yorika; Kuaye,Arnaldo Yoshiteru.
The behaviour of enterotoxin-producing Bacillus cereus in meat was investigated by inoculating spore suspensions of five cultures into meat substrate (pH 5.8) and incubating at 10ºC and 30ºC. The bacterial populations were evaluated after different times by plate counts in nutrient agar. All the cultures presented growth at 30ºC with the generation time varying from 28.8 to 36.0 minutes. Three cultures also presented growth at 10ºC with generation times between 10.16 and 28.38 h. Considering the results, it was concluded that meat kept at abusive temperatures would be subject to development of this microorganism.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Meat; Growth.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400022
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Identification and characterization of the endophytic plant growth prompter Bacillus Cereus strain mq23 isolated from Sophora Alopecuroides root nodules BJM
Zhao,Longfei; Xu,Yajun; Sun,Ran; Deng,Zhenshan; Yang,Wenquan; Wei,Gehong.
Endophytes MQ23 and MQ23R isolated from Sophora alopecuroides root nodules were characterized by observing their ability to promote plant growth and employing molecular analysis techniques. Results showed that MQ23 and MQ23R are potential N2-fixing endophytes and belong to the same species as Bacillus cereus. MQ23 was shown to be able to produce siderophores, IAA, and demonstrate certain antifungal activity to plant pathogenic fungi. Co-inoculation with MQ23+MQ23II showed a more significant effect than inoculation alone in vitro for most of positive actions suggesting they have a cooperative interaction. Results of plant inoculation with endophytes indicated that the growth indexes of co-inoculated MQ23+MQ23II were higher than those of inoculated alone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth promoting characterization; Sophora alopecuroides; Root nodules; Endophytic bacteria; Bacillus cereus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200022
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INDUCED RESISTANCE AND Azospirillum brasilense AT THE CONTROL OF Bipolaris sorokiniana IN WHEAT AND INTERFERENCE ON DIAZOTROPHIC ENDOPHYTES Sci. Agrar.Paran. / SAP
Iurkiv, Luciana; Dildey, Omari Dangelo Forlin; Coltro-Roncato, Sidiane; Gonçalves-Trevisoli, Edilaine Della Valentina; Rissato, Bruna Broti; Guimarães, Vandeir Francisco; Stangarlin, José Renato.
The induced resistance eficiency in the control of phytopathogens is recognized, however little is known about its interference on non-target microrganisms. The present study aims to verify the induction of peroxidase activity by the use of acibenzolar-S-methyl (ASM), mannanoligosaccharide (MOS) and Bacillus cereus inductor in the control of spot blotch wheat disease and its interference on the non-target microrganism A. brasilense. The experiment was conduced under green house growing. The experimental design was made in randomized block design in a factorial schema 4 x 2 x 2, with 4 replications. The first factor "Resistance Inductors" was constituted by ASM, MOS, B. cereus inductors and water (control). The second factor "Pathogen" was constituted by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acibenzolar-S-metil; Bacillus cereus; Indução de resistência; Mananoligossacarídeo fosforilado; Mancha marrom..
Ano: 2017 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14817
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Interference in quality of strawberry caused by the use of biological agents for disease control Sci. Agrar.Paran. / SAP
Heling, A. L.; Favorito, P. A.; Kuhn, O. J..
The strawberry is a fruit of great market acceptance and it is much appreciated throughout the world, besides being very important for small farmers, generating employment and income. Nevertheless, this crop is affected by various diseases that require control, so that producers eventually use chemical fungicides, which, besides contaminating soil and water, eventually contaminate the fruit. In an attempt to reduce the amount of pesticides used in the strawberry crop, a lot of research on biological control of diseases has been done. However, the biological control of diseases in strawberries should not interfere negatively in fruit quality. Thus, this study aimed to evaluate the acidity, pH and total soluble solids (ºBrix) of strawberries treated with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Controle biológico; Saccharomyces boulardii; Saccharomyces cerevisiae.
Ano: 2013 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/7869
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