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Registros recuperados: 16
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Apoptose e expressão de Bcl-2 e das caspases 3 e 8 em placenta bovina, em diferentes estádios de gestação Arq. Bras. Med. Vet. Zootec.
Meça,K.K.O.L.; Martins,A.S.; Del Puerto,H.L; Vasconcelos,A.C..
Apoptose e seus mecanismos reguladores são eventos fisiológicos cruciais para a manutenção da homeostase placentária, e o desequilíbrio desses processos pode comprometer a função placentária e, consequentemente, o sucesso da gravidez. Neste estudo, investigou-se a apoptose utilizando-se histomorfometria em lâminas coradas em HE e submetidas à reação de TUNEL. Além disso, avaliou-se a expressão de Bcl-2 e das caspases 8 e 3, pela reação de polimerase em cadeia em tempo real, em placentas saudáveis em diferentes estádios de gestação. Amostras de placentônios de vacas com quatro, seis e nove meses de gestação foram colhidas e processadas. O índice apoptótico aumentou progressivamente com o avanço da gestação. Tanto o Bcl-2 quanto as caspases 3 e 8 foram...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; Apoptose; Placenta; Caspases; Bcl-2.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200003
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Apoptose na infecção experimental de cães domésticos com Ehrlichia canis Ciência Rural
Sanchez,Irma Ximena Barbosa; Socarras,Teresa de Jesus Oviedo; Silva,Miguel Ladino; Pereira,Nubia Braga; Machado,Rosangela Zacarias; Vasconcelos,Anilton Cesar.
A Erliquiose canina é uma zoonose causada pela Ehrlichia canis, bactéria Gram negativa de distribuição mundial. Alguns cães com erliquiose se tornam portadores assintomáticos enquanto outros desenvolvem uma doença aguda com morte rápida. A apoptose pode ser importante na eliminação de patógenos intracelulares, podendo, nas infecções por Ehrlichia sp. e Anaplasma sp., ocorrer modulação da apoptose celular para prolongar a sobrevivência desses organismos. Para avaliação do papel da apoptose na erliquiose canina, sete cães foram distribuídos em dois grupos. No Grupo inoculado, realizou-se a infecção por via intravenosa com sangue infectado com E. canis (isolado Jaboticabal), sendo realizada a inoculação com PBS estéril nos animais pertencentes ao Grupo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptose; Ehrlichia canis; N-PCR; RIFI; TUNEL; Bcl-2; Bax.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800021
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Effects of silk sericin on the proliferation and apoptosis of colon cancer cells Biol. Res.
Kaewkorn,Waraporn; Limpeanchob,Nanteetip; Tiyaboonchai,Waree; Pongcharoen,Sutatip; Sutheerawattananonda,Manote.
Sericin is a silk protein woven from silkworm cocoons (Bombyx mori). In animal model, sericin has been reported to have anti-tumoral action against colon cancer. The mechanisms underlying the activity of sericin against cancer cells are not fully understood. The present study investigated the effects of sericin on human colorectal cancer SW480 cells compared to normal colonic mucosal FHC cells. Since the size of the sericin protein may be important for its activity, two ranges of molecular weight were tested. Sericin was found to decrease SW480 and FHC cell viability. The small sericin had higher anti-proliferative effects than that of the large sericin in both cell types. Increased apoptosis of SW480 cells is associated with increased caspase-3 activity...
Tipo: Journal article Palavras-chave: Silk sericin; Colon cancer; Apoptosis; Bcl-2; Caspase-3.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100006
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Evaluation of biological activities of Physalis peruviana ethanol extracts and expression of Bcl-2 genes in HeLa cells Ciênc. Tecnol. Aliment.
Çakir,Özgür; Pekmez,Murat; Çepni,Elif; Candar,Bilgin; Fidan,Kerem.
Physalis species are used in folk medicine for phytotherapeutic properties. The extracts of medicinal plants are known to possess cytotoxic and chemopreventative compounds. In this study we investigated antibacterial, antioxidant, DNA damage preventative properties of Physalis peruviana (golden berry) on leaf and shoot ethanol extracts and their effects on cytotoxicity of HeLa cells and expression of apoptotic pathway genes. Among the tested bacteria for antibacterial activity, maximum inhibition zone was determined in Lactococcus lactis. The phenolic content was found higher in leaf extracts than shoot extracts. The antioxidant activity showed the highest TEAC values of the leaf (2 mg/mL) and the shoot (0.5 mg/mL) extracts as 0.291±0.04 and 0.192±0.015,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bcl-2; Antibacterial; Antioxidant; DNA damage; HeLa; Physalis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000200030
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Expression of beclin 1 in primary salivary adenoid cystic carcinoma and its relation to Bcl-2 and p53 and prognosis BJMBR
Jiang,L.C.; Huang,S.Y.; Zhang,D.S.; Zhang,S.H.; Li,W.G.; Zheng,P.H.; Chen,Z.W..
Beclin 1 plays a critical role in autophagy and functions as a haploinsufficient tumor suppressor. The expression and prognostic significance of beclin 1 in head and neck adenoid cystic carcinoma (ACC) are largely unexplored. Therefore, we investigated the expression of beclin 1, Bcl-2, and p53 in head and neck ACC tissue. Tissue samples from 35 cases (15 females, 20 males) of head and neck ACC were utilized for immunohistochemistry. Beclin 1 expression was observed in 32 cases (91.4%) and considered to be high in 15 cases (42.9%) and low in 20 cases (57.1%). Beclin 1 expression was significantly correlated with a histological growth pattern (P=0.046) and histological grade (P=0.037). Beclin 1 expression was inversely correlated with Bcl-2 expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenoid cystic carcinoma; Autophagy; Beclin 1; Bcl-2; P53.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000300252
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Fludarabine induces apoptosis in chronic lymphocytic leukemia - the role of P53, Bcl-2, Bax, Mcl-1, and Bag-1 proteins BJMBR
Faria,J.R.; Yamamoto,M.; Faria,R.M.D.; Kerbauy,J.; Oliveira,J.S.R..
The expression of P53, Bcl-2, Bax, Bag-1, and Mcl-1 proteins in CD5/CD20-positive B-chronic lymphocytic leukemia (B-CLL) cells from 30 typical CLL patients was evaluated before and after 48 h of incubation with 10-6 M fludarabine using multiparametric flow cytometric analysis. Protein expression was correlated with annexin V expression, Rai modified clinical staging, lymphocyte doubling time, and previous treatment. Our main goal was to determine the predictive value of these proteins in CLL cells in terms of disease evolution. Bcl-2 expression decreased from a median fluorescence index (MFI) of 331.71 ± 42.2 to 245.81 ± 52.2 (P < 0.001) after fludarabine treatment, but there was no difference between viable cells (331.57 ± 44.6 MFI) and apoptotic cells...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chronic lymphocytic leukemia; Apoptosis; Fludarabine; P53; Bcl-2; Bax; Mcl-1; Bag-1.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300003
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Immunohistochemical investigation of neuronal injury in cerebral cortex of cobra-envenomed rats J. Venom. Anim. Toxins incl. Trop. Dis.
Rahmy,T.R.; Hassona,I.A..
The immunohistochemical expression of neuron-specific enolase, NSE (a cytoplasmic glycolytic enzyme of the neurons), synaptophysin, SYN (a major membrane glycoprotein of synaptic vesicles), and Bcl-2 (anti-apoptotic protein) were determined in cerebral cortex of rats envenomed with neurotoxic venom from Egyptian cobra. Male rats were intramuscularly (IM) injected with a single injection of either physiological saline solution or ½ LD50 or LD50 of cobra venom and sacrificed 24, 48, or 72 hr after envenoming. Formalin-fixed paraffin sections were immunohistochemically studied by avidin-biotin-peroxidase complex method. Neuron histological structure and isolation of genomic DNA were also detected. The results showed a dose and time-dependent increase in NSE...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunohistochemistry; Neuron specific enolase; Synaptophysin; Bcl-2; Cerebral cortex; Cobra snake venom.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000100005
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Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis International Journal of Morphology
Novais,P. C; de Paula,A. A. P; Reis,R. B; Barione,D. F; Franqueiro,A. G; Rodrigues Jr,A. A; Lizarte,F. S. N; de Carvalho,C. A. M; Tirapelli,D. P. C; Peria,F. M; Saggioro,F. P; Tucci Jr,S; Cologna,A. J; Martins,A. C. P; Tirapelli,L. F.
The aim was to analyze the protein expression of apoptotic genes caspase-3, caspase-8 and bcl-2 with the immunohistochemistry technique, correlating with tumor grade (I, II and III) and with the patient survival in order to understand the basic mechanism of tumoral transformation. The immunohistochemistry reactions on 50 samples of squamous cell carcinoma were carried out with the avidin-biotin immunoperoxidase method and antigen recovery. The analyses were made using the graduation method "in crosses" (0 to 4 crosses - no stain to more than 75% of positives cells) and in categories (low, intermediate, high) of the cytoplasm immunoreactivity of the epidermoid penile carcinoma cells. It was observed a statistically significant difference when the expression...
Tipo: Journal article Palavras-chave: Bcl-2; Caspase-3; Caspase-8; Penile cancer; Apoptosis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300043
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In vitro modulation of Bcl-2 levels in small cell lung cancer cells: effects on cell viability BJMBR
Santos,A.O.; Pereira,J.P.; Pedroso de Lima,M.C.; Simões,S.; Moreira,J.N..
Small cell lung cancer (SCLC) is an aggressive disease, representing 15% of all cases of lung cancer, has high metastatic potential and low prognosis that urgently demands the development of novel therapeutic approaches. One of the proposed approaches has been the down-regulation of BCL2, with poorly clarified and controversial therapeutic value regarding SCLC. The use of anti-BCL2 small interfering RNA (siRNA) in SCLC has never been reported. The aim of the present study was to select and test the in vitro efficacy of anti-BCL2 siRNA sequences against the protein and mRNA levels of SCLC cells, and their effects on cytotoxicity and chemosensitization. Two anti-BCL2 siRNAs and the anti-BCL2 G3139 oligodeoxynucleotide (ODN) were evaluated in SCLC cells by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Small cell lung cancer; Gene silencing; Bcl-2; SiRNA; Antisense ODN; Flow cytometry; Chemosensitization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000013
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Inmunohistochemical Expression of PCNA, p53 and bcl-2 in Pleomorphic Adenomas International Journal of Morphology
Gordón-Núñez,Manuel Antonio; Godoy,Gustavo Pina; Soares,Rosilene Calazans; Souza,Lélia Batista de; Freitas,Roseana de Almeida; Queiroz,Lélia María Guedes.
The aim of the study was to determine the immunohistochemical expression of the PCNA, p53 and bcl-2 proteins in pleomorphic adenomas.onineteen specimens of pleomorphic adenomas were selected for analysis by the streptavidin-biotin-peroxidase method with antibodies againstp53, PCNA and bcl-2 proteins. It was observedweak labeling for p53 in 12 cases (63.1%) andforPCNA in 8 (42.1%). With respect to the bcl-2 labeling index,ono expression of this protein was detected in 12 cases, corresponding to 63.1% of the sample. Based on these findings, it was concluded that p53 and PCNA can favor the proliferative activity of pleomorphic adenomas, whereas bcl-2 probably doesonot effectively particípate in the pathogenesis of this tumor.
Tipo: Journal article Palavras-chave: Pleomorphic adenoma; Salivary gland tumour; P53; PCNA; Bcl-2.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000300010
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Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro BJMBR
Yang,H.; Zhu,Y.T.; Cheng,R.; Shao,M.Y.; Fu,Z.S.; Cheng,L.; Wang,F.M.; Hu,T..
Lipopolysaccharide exerts many effects on many cell lines, including cytokine secretion, and cell apoptosis and necrosis. We investigated the in vitro effects of lipopolysaccharide on apoptosis of cultured human dental pulp cells and the expression of Bcl-2 and Bax. Dental pulp cells showed morphologies typical of apoptosis after exposure to lipopolysaccharide. Flow cytometry showed that the rate of apoptosis of human dental pulp cells increased with increasing lipopolysaccharide concentration. Compared with controls, lipopolysaccharide promoted pulp cell apoptosis (P < 0.05) from 0.1 to 100 μg/mL but not at 0.01 μg/mL. Cell apoptosis was statistically higher after exposure to lipopolysaccharide for 3 days compared with 1 day, but no difference was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dental pulp cells; Lipopolysaccharide; Apoptosis; Bax; Bcl-2.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100003
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Mitochondrial Ca2+ homeostasis in health and disease Biol. Res.
CAMPANELLA,MICHELANGELO; PINTON,PAOLO; RIZZUTO,ROSARIO.
Although it has long been known that mitochondria possess a complex molecular repertoire for accumulating and releasing Ca2+, only in recent years has a large body of data demonstrated that these organelles promptly respond to Ca2+-mediated cell stimulations. In this contribution, we will review the principles of mitochondrial Ca2+ homeostasis and its signaling role in different physiological and pathological conditions.
Tipo: Journal article Palavras-chave: Mitochondria; Calcium; Apoptosis; Bcl-2; Viral proteins.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400022
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Protective effect of crocetin from Crocus sativus L. on myocardial ischemia-reperfusion injury in rats Ciênc. Tecnol. Aliment.
LIU,Chengxiao; SUN,Erlian; MENG,Wenjun; SUN,Guoqing.
Abstract This study investigated the protective effect of crocetin from Crocus sativus L. on myocardial ischemia-reperfusion injury (MIRI) in rats. Sixty SD rats were randomly divided into sham-operated, model, and low-, medium- and high-dose crocetin groups. Later 3 groups were intragastrically administrated with 10, 20 and 40 mg/kg crocetin from Crocus sativus L., respectively, for 1 week. On the 8th day, the MIRI model was established in the later 4 groups. The blood biochemical indexes, hemodynamic indexes, myocardial infarct size, myocardial antioxidant indexes and myocardial expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), serine/threonine kinase (Akt) and phosphorylated Akt (p-Akt) protein were determined. Results showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crocetin; Ischemia-reperfusion injury; Bcl-2; Bax; Akt.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600595
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The anesthetic agent sevoflurane attenuates pulmonary acute lung injury by modulating apoptotic pathways BJMBR
Wang,L.; Ye,Y.; Su,H.B.; Yang,J.P..
The objective of this study was to evaluate lung protection by the volatile anesthetic sevoflurane (SEVO), which inhibits apoptosis. Male Sprague-Dawley rats (250–280 g; n=18) were randomly divided into three groups. The LPS group received 5 mg/kg endotoxin (lipopolysaccharide), which induced acute lung injury (ALI). The control (CTRL) group received normal saline and the SEVO group received sevoflurane (2.5%) for 30 min after ALI was induced by 5 mg/kg LPS. Samples were collected for analysis 12 h after LPS. Lung injury was assessed by pathological observations and tissue wet to dry weight (W/D) ratios. Apoptotic index (AI) was determined by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and electron microscopy. Caspase-3 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sevofluran; Apoptosis; Pulmonary acute lung injury; Caspase-3; Bcl-2.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300702
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The regulation of apoptotic cell death BJMBR
Amarante-Mendes,G.P.; Green,D.R..
Apoptosis is a fundamental biological phenomenon in which the death of a cell is genetically and biochemically regulated. Different molecules are involved in the regulation of the apoptotic process. Death receptors, coupled to distinct members of the caspases as well as other adapter molecules, are involved in the initiation of the stress signals (The Indictment). Members of the Bcl-2 family control at the mitochondrial level the decision between life and death (The Judgement). The effector caspases are responsible for all morphological and biochemical changes related to apoptosis including the "eat-me" signals perceived by phagocytes and neighboring cells (The Execution). Finally, apoptosis would have little biological significance without the recognition...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Cell death; Caspase; Mitochondria; Bcl-2; Oncogene.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900001
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The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma International Journal of Morphology
Hongfu,Liu; Luping,Zhang; Shaojie,Chen; Zengxian,Wang; Fei,Huang; Dong,Wang.
This study aimed to observe the regularity of liver cell apoptosis and expression of apoptosis related gene Bcl-2 and Bax in the induced cancer, and explore the relationship between apoptosis and the development of liver cancer. In this study, 84 rats were used, 72 rats as experimental group induced by drinking water containing DEN, 12 rats as control group (CG). After laparotomy, the form, color, texture of the liver and metastatic tumor in both control and experimental groups were observed and recorded. The metastatic tumor and the liver tissue were sectioned and stained with hematoxylin-eosin staining to demonstrate the characteristic in morphological changes, the Hoechst 33342 fluorescent staining was applied to show and count the rate of apoptotic...
Tipo: Journal article Palavras-chave: Primary hepatic carcinoma; Apoptosis; Bcl-2; Bax; Rats.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400032
Registros recuperados: 16
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