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Registros recuperados: 23
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A rapid quantitative fluorescence-based bioassay to study allelochemical interactions from Alexandrium minutum ArchiMer
Long, Marc; Tallec, Kevin; Soudant, Philippe; Lambert, Christophe; Le Grand, Fabienne; Sarthou, Geraldine; Jolley, Dianne; Hegaret, Helene.
Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods....
Tipo: Text Palavras-chave: Harmful algal bloom; Alexandrium; Allelopathy; Cytotoxicity; PAM fluorometry; Bioassay.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00453/56424/58116.pdf
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Ação alelopática de sementes de Canavalia ensiformis contra Cyperus rotundus. Repositório Alice
FORSTER, R.; SAITO, M.L.; MORICONI, W..
Ação alelopática da leguminosa Canavalia ensiformis (feijão de porco) já é praticada em campo semeado com essa leguminosa. Tentativa de esclarecer o modo de ação, levou a testar as partes, raízes, caules, folhas moídas misturadas a terra, plantando-se em seguida os tubérculos de Cyperus rotundus L. em ambiente de laboratório ou em casa de vegetação. Utilizando copos plásticos com capacidade de 160ml, terra argilosa misturada com quantidades diferentes das partes acima citadas, não revelaram qualquer ação sobre a capacidade de brotação dos tubérculos do Cyperus. Utilizando o mesmo método com vagens secas do feijão de porco, e separadamente com sementes, foi possível observar pequena ação da vagem seca, e pronunciada ação das sementes moídas, observou que...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Alelopatia; Canavalia ensiformis; Cyperus rotundus; Leguminosa; Tiririca; Planta daninha; Planta invasora; Elemento ativo; Bioensaio; Allelopathy; Leguminosae; Weed; Bioassay; Active agent.
Ano: 1995 URL: http://www.alice.cnptia.embrapa.br/handle/doc/12420
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Acaricidal activity of extracts from different structures of Piper tuberculatum against larvae and adults of Rhipicephalus microplus. Repositório Alice
BRAGA, A. G. S.; SOUZA, K. F. A. de; BARBIERI, F. da S.; FERNANDES, C. de F.; ROCHA, R. B.; VIEIRA JUNIOR, J. R.; LACERDA, C. L.; CELESTINO, C. O.; FACUNDO, V. A.; BRITO, L. G..
Estratégias de controle do carrapato dos bovinos, Rhipicephalus microplus, fundamentam-se na utilização de pesticidas. Os principais grupos químicos utilizados atualmente mostram-se ineficazes devido ao surgimento de populações resistentes. A pesquisa de novas moléculas com eficiência acaricida é uma necessidade para manutenção da produtividade dos rebanhos bovinos estabelecidos em regiões de clima tropical. Avaliamos a atividade de Piper tuberculatum para o controle de fêmeas ingurgitadas e larvas de R. microplus através de bioensaios de imersão de adultos e de pacotes de larvas. As concentrações avaliadas foram de 50; 25; 12,50; 6,25; 3,12 e 1,56 mg mL-1 de extrato etanólico de folha, talo e fruto de P. tuberculatum. As concentrações letais para 50% dos...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Carrapato dos bovinos; Bioensaios; Biomoléculas ativas; Active molecules; Bioassay; Cattle tick; Piperaceae.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088637
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Active biological materials inhibiting tumor initiation extracted from marine algae OceanDocs
Mostafa, M.H.; Ibrahim, A.M.M.; El-Masry, M.H.; El-Naggar, M.A..
Ethanol extraction of seventeen algal species from 35 different samples collected from the coastal area of Abou-Qir, Egypt, South East of the Mediterranean Sea Coast, was conducted. The aim was to obtain natural products from the algal extracts which have significant biologically active algal compounds. The collected algae showed a wide variation in their occurrence during the period of collection. Thirty five extracts representing different seasonal growths of 17 marine algal species were tested for antitumorigenic activity. Tests performed were the toxicity bioassay using Brine shrimp Artemia salina and the anti-tumor bioassay against Agrobacterium tumefaciens on potato discs. Codium tomentosum (winter); Jaina rubens (summer) and Padina pavonia (winter)...
Tipo: Journal Contribution Palavras-chave: Anti-tumor agent; Bioactive material; Bioassay; Marine algae; Natural product compound Biological properties Algae Marine environment http://aims.fao.org/aos/agrovoc/c_4610.
Ano: 2005 URL: http://hdl.handle.net/1834/1303
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Chronic toxicity bioassay with populations of the crustacean Artemia salina exposed to the organophosphate diazinon Biol. Res.
Bustos-Obregon,Eduardo; Vargas,Álvaro.
A chronic toxicity bioassay was conducted with the microcrustacean Artemia salina as the testing organism for the toxic organophosphate diazinon in order to determine if the species is an appropriate indicator of pollution in aquatic environments. Tests of animal exposure to different concentrations of the toxicant were performed for 24, 48 and 72 hours after larvae hatching. Registered mortality data was used to obtain the lethal dose 50 (LD50) of the organophosphate for each exposure time, considering the immobilization of A. salina larvae as the mortality parameter. The lethal concentration (LD50) in the same exposure times was calculated by evaluating morphological changes on the three initial stages of larval development. Both doses were determined by...
Tipo: Journal article Palavras-chave: Diazinon; Artemia salina; Ecotoxicology; Bioassay.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300013
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Early-Stage Bioassay for Monitoring Radioactive Contamination in Living Livestock OAK
YAMAGUCHI, Toshiro; SAWANO, Kaita; KISHIMOTO, Miori; FURUHAMA, Kazuhisa; YAMADA, Kazutaka; 山田, 一孝.
Soil samples from the ground surface and feces and blood from a mixed-breed male pig were collected on April 10, 2011 at a farm within 20 km of the Fukushima Daiichi nuclear power plant. The radioactivity of each sample was measured using a Ge semiconductor detector. Despite the fact that the pig had been fed non-contaminated imported feed, 131I, 134Cs and 137Cs were detected in the feces, and 134Cs and 137Cs were detected in the blood clots. Because it is considerably difficult to measure radioactive contamination in the edible muscle of living livestock, bioassays are an option for the screening of radioactive contamination in living livestock to ensure food safety.
Palavras-chave: Bioassay; Cesium; Fukushima; Iodine; Radioactive contamination.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3893
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Effects of dissolved mercury on embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae ArchiMer
Beiras, R; His, Edouard.
The effects of Hg concentrations ranging from 0 (control) to 1024 mu g l(-1) upon embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae were investigated. Embryogenesis was abnormal in 50% of the individuals at 10 mu g l(-1). The 48 h LC(50) for D-shaped, early umbonate, late umbonate and eyed larvae were 51, 164, 322 and 383 pg l(-1) respectively. The LC(50) was an allometric function of ash-free dry weight with exponent b = 0.60. Larval growth was significantly reduced after 6 d exposure to 4 mu g Hg l(-1) and after 10 d exposure to 2 mu g l(-1). No significant differences in lethal or sublethal sensitivity to Hg were found between M. galloprovincialis and previously studied Crassostrea gigas embryos and larvae. The implications...
Tipo: Text Palavras-chave: Mercury; Mytilus; Mussel; Larva; Growth; Embryo; Bioassay; Ecotoxicology.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00258/36946/35665.pdf
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Effects of dissolved mercury on embryogenesis, survival, growth and metamorphosis of Crassostrea gigas oyster larvae ArchiMer
Beiras, Ricardo; His, Edouard.
The effects of mercury (Hg) concentrations ranging from 0 (control) to 1024 µg 1-1 upon embryogenesis, survival, growth and metamorphosis of Crassostrea gigas (Thunberg) oyster larvae were investigated. Embryogenesis was abnormal in 50 % of the individuals at 11 µg 1-1 . The 48 h LD50 for D·shaped, umbonate and pediveliger larvae were 33, 115 and 200 µg 1-1 respectively. The increase in LD50 was partially explained by the larval weight increase, although weight-specific tolerance to Hg was higher in smaller larvae. Growth, the most sensitive physiological process studied, was significantly retarded at 4 µg 1-1. The metamorphosis rate was significantly reduced when competent pediveligers were exposed to 64 µg 1-1 for 48 h prior to the addition of the...
Tipo: Text Palavras-chave: Mercury; Oyster; Larvae; Embryo; Bioassay; Ecotoxicology; Crassostrea gigas.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00037/14867/12191.pdf
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Effects of dissolved mercury on embryogenesis, survival, growth of Mytilus galloprovincialis mussel larvae ArchiMer
Beiras, Ricardo; His, Edouard.
The effects of Hg concentrations ranging from 0 (control) to 1024 µg 1-1 upon embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae were investigated . Embryogenesis was abnormal in 50 % of the individuals at 10µg 1-1. The 48 h LC50 for D-shaped, early umbonate, late umbonate and eyed larvae were 51, 164, 322 and 383 µg 1- 1 respectively. The LC50 was an allometric function of ash-free dry weight with exponent b = 0.60. Larval growth was significantly reduced alter 6 d exposure to 4 µg Hg 1-1 and alter 10 d exposure to 2 µg 1-1. No significant differences in lethal or sublethal sensitivity to Hg were found between M. galloprovincialis and previously studied Crassostrea gigas embryos and larvae. The implications of these findings for...
Tipo: Text Palavras-chave: Mercury; Mytilus galloprovincialis; Mussel; Larvae; Growth; Embryonic development; Bioassay; Ecotoxicology.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00037/14868/12192.pdf
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Eléments nutritifs limitant la biomasse phytoplanctonique d'une lagune tropicale de Côte d'Ivoire. Comparaison des méthodes d'estimation ArchiMer
Dufour, P.; Cremoux, Jl.; Slepoukha, M..
"In vitro" enrichment experiments on natural populations have been used to determine the nutrients controlling the potential biomass of phytoplancton in the Ebrié coastal lagoon. The limiting nutrients were generally nitrogen, phosphorus and carbon, in this order ; in three cases out of eleven, P and N act simultaneously ; in an extreme eutrophication case, N, P and C were all limiting. No effect was observed following addition of metals, vitamins or chelators. The limitations deduced from the enrichment experiments show a. good agreement with the elementary composition of the external medium ; in waters potentially limited by N alone, the dissolved inorganic nutrients have a N : P ratio lower than 25. The agreement is poorer when the sestonic N : P ratio...
Tipo: Text Palavras-chave: Lagune; Estuaire; Zone tropicale; Phytoplancton; Biomasse; Eléments limitants; Tests biologiques; Composition chimique; Coastal lagoon; Estuary tropical zone; Phytoplankton; Biomass; Limiting nutrients; Bioassay; Chemlcal composition.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00306/41689/40898.pdf
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Evaluation du potentiel nutritif des eaux de la baie de Vilaine pendant l'été 1987 ArchiMer
Le Dean, Loic; Truquet, Philippe; Lassus, Patrick.
The growth potential study of waters of the Vilaine bay was realized from march to september 1987 and for three sampling stations. The growth of depleted test cells of Skeletonema costatum was estimated and compared from existing nutrients pools for aIl water samples. As a first result it appears that inorganic nitrogen could stimulate the growth during spring while no clear relationship is observed between the algal growth and other hydrological parameters during spring or summer periods. Nevertheless these first results need further corroboration.
Tipo: Text Palavras-chave: Potentiel nutritif; Baie de Vilaine; Skeletonema costatum; Test biologique; Growth potential; Vilaine Bay; S. costatum; Bioassay.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00139/25037/23146.pdf
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Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds ArchiMer
Réveillon, Damien; Tunin-ley, Alina; Grondin, Isabelle; Othmani, Ahlem; Zubia, Mayalen; Bunet, Robert; Turquet, Jean; Culioli, Gérald; Briand, Jean-françois.
Marine microalgae and cyanobacteria have largely been studied for their biotechnological potential and proved their ability to produce a wide array of bioactive molecules. We investigated the antifouling potential of unexplored benthic tropical microalgae using anti-adhesion and toxicity bioassays against two major micro- and ma crobiofoulers, namely bacteria and barnacles. Fifty strains belonging to six phyla [Cyanobacteria, Miozoa (Dinoflagellata), Bacillariophyta, Cryptophyta, Rhodophyta and Haptophyta] were isolated from southwestern Islands of the Indian Ocean. They were chosen in order to represent as much as possible the huge biodiversity of such a rich tropical ecosystem. The associated chemodiversity was highlighted by both NMR- and LC-MS-based...
Tipo: Text Palavras-chave: Microalgae; Antifouling; Bioassay; Chemodiversity; Bioprospecting; Metabolomics.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00454/56608/58333.pdf
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FACTORS INFLUENCING THE RESPONSES OF Tetranychus urticae Koch (ACARINA: TETRANYCHIDAE) TO THURINGIENSIN Agricultura Técnica
Vargas,Robinson; Chapman,Bruce; Penman,David R..
The effects of temperature, host plant, active ingredient mobility, surfactant, residue age and larval age on the response of Tetranychus urticae Koch to thuringiensin were evaluated in laboratory bioassays. T. urticae larvae were significantly more susceptible to thuringiensin at 13°C than at 28°C. A significantly different response of T. urticae larvae to thuringiensin residues was found on peach and apple leaves. Older T. urticae larvae were significantly more susceptible than younger larvae when exposed to thuringiensin residues. No systemic or translaminar effects of thuringiensin were detected; the leaf surface (upper or lower) and the addition of Silwet L-77 surfactant did not affect the efficacy of thuringiensin. The use of thuringiensin in spider...
Tipo: Journal article Palavras-chave: Beta -exotoxin; Miticide; Bioassay; Pesticide; Residue age; Surfactant; Translocation.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072002000100001
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Implementation of an in vitro bioassay as an indicator of the bionutrient FitoMas E Ciencia e Investigación Agraria
Díaz de Villegas,María Elena; Delgado,Grizel; Rivas,Mauricio; Torres,Esmérida; Saura,Maribel.
Seed bioassays are employed to determine the stimulatory effect of various biological products on the seed germination of plants used for the detection and control of toxic environmental contaminants. The objective of the present study was to design an in vitro bioassay to monitor the quality of the final production of FitoMas E, a bionutrient derived from the sugar industry and produced by the Instituto Cubano de Investigaciones de los derivados de la Caña de Azúcar By-Products (ICIDCA), by determining rates of seed germination and growth of lettuce (Lactuca sativa L.) as an indicator plant. A significant effect of controllable factors under study (FitoMas E concentration in lots and replicates of Petri dishes) was determined by one-way and multifactor...
Tipo: Journal article Palavras-chave: Bioassay; Bionutrient; Lettuce seed.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000200005
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Insects as biological models to assay spider and scorpion venom toxicity J. Venom. Anim. Toxins incl. Trop. Dis.
Manzoli-Palma,M. F.; Gobbi,N.; Palma,M. S..
This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioassay; Venom; Insect model; Toxicity; Apis mellifera.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200004
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Integrated indicator framework and methodology for monitoring and assessment of hazardous substances and their effects in the marine environment ArchiMer
Vethaak, A. Dick; Davies, Ian M.; Thain, John E.; Gubbins, Matthew J.; Martinez-gomez, Concepcion; Robinson, Craig D.; Moffat, Colin F.; Burgeot, Thierry; Maes, Thomas; Wosniok, Werner; Giltrap, Michelle; Lang, Thomas; Hylland, Ketil.
Many maritime countries in Europe have implemented marine environmental monitoring programmes which include the measurement of chemical contaminants and related biological effects. How best to integrate data obtained in these two types of monitoring into meaningful assessments has been the subject of recent efforts by the International Council for Exploration of the Sea (ICES) Expert Groups. Work within these groups has concentrated on defining a core set of chemical and biological endpoints that can be used across maritime areas, defining confounding factors, supporting parameters and protocols for measurement. The framework comprised markers for concentrations of, exposure to and effects from, contaminants. Most importantly, assessment criteria for...
Tipo: Text Palavras-chave: Chemical measurements; Biomarker; Bioassay; Pollution effects; Biomonitoring; Environmental; Impact; MSFD; ICES; OSPAR.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00283/39429/37869.pdf
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INTEGRATING CHEMICAL AND BIOLOGICAL STATUS ASSESSMENT: ASSEMBLING LINES OF EVIDENCE FOR THE EVALUATION OF RIVER ECOSYSTEM RISK Acta biol.Colomb.
MUÑOZ,ISABEL; SABATER,SERGI.
This paper describes various approaches to evaluating ecological risk in rivers affected by multiple chemical stressors, with emphasis on biofilm and invertebrate community responses. Metrics should be considered as different lines of evidence that, when weighted, form an advanced weight of evidence approach to establishing the environmental risk on a basin scale. Combination of field surveys to obtain observational data of communities, in situ experiments, toxicological sediment tests and a good chemical description of the medium (water and sediment) helps give an integrative view of the chemical and biological state of a river ecosystem. The greater and more distinct the variables used, the greater is our ability to identify the effects of major...
Tipo: Journal article Palavras-chave: Bioassay; Chemical indicators; Ecological status; Weight of evidence.
Ano: 2014 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2014000100003
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Limiting nutrients of oyster pond seawater in the Marennes-Oleron region for Haslea ostrearia : applications to the mass production of the diatom in mesocosom experiments ArchiMer
Turpin, Vincent; Robert, Jean-michel; Goulletquer, Philippe.
Bioassays were carried out with the 'blue diatom' Haslea ostrearia Simonsen, which is responsible for oyster greening during the fattening period of Crassostrea gigas Thunberg in oyster ponds. Samples of seawater were taken from two oyster ponds: one without oysters and the other with 20 oysters per m_, maximal density allowed by the French AFNOR norm for 'refinement'. The aims were to clarify the nutrient requirements of this diatom, also to elucidate the eventual influence of C. gigas at this density on the seawater fertility and to envisage the mass production of this diatom by pond fertilization. Examination of cell numeric densities at the end of bioassays allows us to conclude that silicate was the first limiting nutrient, closely followed by...
Tipo: Text Palavras-chave: Marennes Oleron; Oyster ponds; Mesocosm experiment; Bioassay; Haslea ostrearia; Bacillariophyceae; Limiting nutrients; Marennes Oléron; Claires à huîtres; Expérience en mésocosmes; Bioessai; Haslea ostrearia; Bacillariophyceae; Nutriments limitants.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-3096.pdf
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Molecular frequency and isolation of cyst-forming coccidia from free ranging chickens in Bahia State, Brazil. Repositório Alice
GONCALVES, I. N.; UZÊDA, R. S.; LACERDA, G. A.; MOREIRA, R. R. N.; ARAUJO, F. R.; OLIVEIRA, R. H. M. de; CORBELINE, L. G.; GODIM, L. F. P..
2012
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Toxoplasma gondii; Neospora caninum; Hammondia spp.; PCR; IFAT; Bioassay.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937536
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Mytilus galloprovincialis as bioindicator in embryotoxicity testing to evaluate sediment quality in the lagoon of Venice (Italy) ArchiMer
Annamaria, V; Chiara, L; Alessandra, A; Alvise, B; His, Edouard; Francesco, G.
The possibility of using Mytilus galloprovincialis from natural populations of the lagoon of Venice in toxicity bioassays based on embryo development, according to international standard methods, was investigated. In order to valuate this method for the lagoon, iterative steps including evaluation of reproducibility, sensitivity and discriminatory capacity towards some pure substances and environmental samples were performed. Evaluation of sensitivity towards a reference toxicant (copper) evidenced good replicability and repeatability. Results showed that natural population is better than hatchery population for performing bioassays. Evaluation of applicability to elutriates revealed that the method is able to discriminate among sediments of the lagoon of...
Tipo: Text Palavras-chave: Elutriates; Autochthonous population; Reproducibility; Bioassay.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-4200.pdf
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