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02SRC154 eCrystals
Allen, David W.; Coles, Simon J.; Hursthouse, Michael B..
Tipo: Crystal Structure Data Holding Palavras-chave: C~33~H~~25~BrNO~2~P; Organic; Biosensors; Polar compounds organic; Electronic materials.
Ano: 2002 URL: http://ecrystals.chem.soton.ac.uk/878/
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Assessment of molecular recognition element for the quantification of human epidermal growth factor using surface plasmon resonance Electron. J. Biotechnol.
Rosti,Ira Amira; Ramanan,Ramakrishnan Nagasundara; Ling,Tau Chuan; Ariff,Arbakariya B.
Background: A method for the selection of suitable molecular recognition element (MRE) for the quantification of human epidermal growth factor (hEGF) using surface plasmon resonance (SPR) is presented. Two types of hEGF antibody, monoclonal and polyclonal, were immobilized on the surface of chip and validated for its characteristics and performance in the quantification of hEGF. Validation of this analytical procedure was to demonstrate the stability and suitability of antibody for the quantification of target protein. Results: Specificity, accuracy and precision for all samples were within acceptable limit for both antibodies. The affinity and kinetic constant of antibodies-hEGF binding were evaluated using a 1:1 Langmuir interaction model. The model...
Tipo: Journal article Palavras-chave: Antibody; BIAcore; Biosensors; Epidermal growth factor; Validation.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600015
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DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant? ArchiMer
Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R..
The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and...
Tipo: Text Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf
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Efeito in vitro de poluentes inorgânicos usados na agricultura sobre a atividade da catalase da alga Pseudokirchneriella subcapitata. Repositório Alice
DUARTE, S.; AMARAL SOBRINHO, N. M. B.; JONSSON, C. M.; JOKL, L..
Resumo: O efeito de poluentes em ecossistemas pode ser verifi cado pela sua ação sobre os produtores primários, como a alga Pseudokirchneriella subcapitata (Korshikov) F. Hindák (Chlorophyceae), que tem ampla distribuição em águas dulcícolas e no solo. O efeito de oito íons metálicos empregados na agricultura (alumínio, cádmio, chumbo, magnésio, manganês, mercúrio, selênio e zinco) sobre a atividade enzimática em P. subcapitata foi estudado, usando a catalase como marcador bioquímico. Alumínio, chumbo, mercúrio e zinco foram considerados inibidores e magnésio e manganês como ativadores da ação enzimática. A atividade da catalase na presença de cádmio e selênio não foi alterada. A atividade da catalase pode ser uma ferramenta promissora como biossensor...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biosensor; Íons metálicos; Atividade enzimática; Pseudokirchneriella; Alga; Poluente; Impacto ambiental; Metal pesado; Algae; Biosensors; Catalase; Pollutants; Environmental impact.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1009712
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Electrochemical biosensors in pharmaceutical analysis BJPS
Gil,Eric de Souza; Melo,Giselle Rodrigues de.
Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensors; Pharmaceutical analysis; Electrochemical sensors; Enzymes.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300002
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Partial purification and thermal stability of two peroxidases from Pithecellobium dulce (Roxb.) Benth. Aril. Repositório Alice
JULIÃO, M. M. S.; OLIVEIRA, S. T.; ANDRADE, L. B. S.; FIGUEIREDO, M. F.; SALLES, H. O..
Abstract: Two peroxidase (POX) forms, named PdPI and PdPII, were partially purified from the Pithecellobium dulce aril, using ammonium sulfate precipitation and anion exchange chromatography. Zymography of the proteins obtained after precipitation in the range of 60-90% ammonium sulfate (F6090 fraction) showed two bands with peroxidase activity. In DE-52 cellulose column, the peroxidase activity was detected in unadsorbed peak (PdPI) and adsorbed peak eluted with 0.2 mol L-1 NaCl (PdPII) suggesting that PdPI (~114 KDa) and PdPII (~77 KDa) are basic and acidic proteins, respectively, being PdPI stable at 80 ºC. This is the first report of partial purification of POX from P. dulce aril, indicating that this species may be a new source of enzymes for use in...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Atividade peroxidásica; Zimograma; Biossensor; Enzima; Pithecellobium dulce; Biosensors.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1091021
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The application of polymerized lipid vesicles as colorimetric biosensors for real-time detection of pathogens in drinking water Electron. J. Biotechnol.
Villalobos,Patricio; Chávez,María Isabel; Olguín,Yusser; Sánchez,Elizabeth; Valdés,Erika; Galindo,Raúl; Young,Manuel E.
The inadequate treatments given to the served waste water which are disposal to the rivers and sea coast are the major sources of faecal Microorganisms and enteric bacterial pathogens. They are among the most serious effects of water pollution bringing risks on public health. None of the current methods for detection of pathogens offer real-time on site solutions, are capable of delivering a simple visual detection signal, or can be easily instrumented as an indicator of the presence of a pathogen in water. The use of lipid vesicles incorporating Polydiacetylenes (PDAs) for the development of biosensors for “real-time” detection of pathogens has become an alternative, due to its potential for simple colorimetric response against harmful...
Tipo: Journal article Palavras-chave: Bacterial detection; Biosensors; Lipid vesicles; PDA.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100004
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Tools for detecting insect semiochemicals: a review. Repositório Alice
BREZOLIN, A. N.; MARTINAZZO, J.; MUENCHEN, D. K.; CEZARO, E. M. de; RIGO, A. A.; STEFFENS, C.; STEFFENS, J.; MORAES, M. C. B.; BORGES, M..
bitstream/item/181538/1/Brezolin2018-Article-ToolsForDetectingInsectSemioch.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pest insects; Agriculture; Biosensors; Insect pheromones.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094178
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Use of Enzymatic Biosensors as Quality Indices: A Synopsis of Present and Future Trends in The Food Industry Chilean J. Agric. Res.
Serna Cock,Liliana; Zetty Arenas,Ana María; Ayala Aponte,Alfredo.
Biosensors are an important alternative in the food industry to ensure the quality and safety of products and process controls with effective, fast and economical methods. Their technology is based on a specific biological recognition element in combination with a transducer for signal processing. The use of enzymatic biosensor technology in food processing, quality control and on-line processes is promising compared to conventional analytical techniques, as it offers great advantages due to size, cost, specificity, fast response, precision and sensitivity. This article reviews the development and use of some enzyme biosensors in the food industry, describes the most important application areas and analyzes the current situation and future possibilities....
Tipo: Journal article Palavras-chave: Biosensors; Enzymes; Analysis in food; Safety; Quality; Control processes.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200017
Registros recuperados: 9
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