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*100 Semi-supercentenarians and older as a proposed sample set for the Archon Genomics X PRIZE Validation Protocol* Nature Precedings
Thomas Perls; Nir Barzilai.
The "Archon Genomics X PRIZE Validation Protocol":http://precedings.nature.com/documents/5731/version/1 has been posted in part to solicit comments from the scientific community. Here, we suggest 100 subjects age >=105 years as the source of DNAs for the competition.

Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Data Standards.
Ano: 2011 URL: http://precedings.nature.com/documents/5756/version/1
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12x coverage 454/Sanger Hybrid Assembly of Cucumber (Cucumis sativus L. cv.Borszczagovski) genome - the most efficient way to begin post genomic era Nature Precedings
Rafał Wóycicki; Justyna Witkowicz; Magdalena Pawełkowicz; Ewa Siedlecka; Wojciech Gutman; Wojciech Pląder; Anna Seroczyńska; Mieczysław Śmiech; Katarzyna Niemirowicz-Szczytt; Stanisław Karpiński; Stefan Malepszy; Zbigniew Przybecki.
New sequencing technologies make it possible to achieve genome sequence fast and cheap. Since the assembly step of such next generation reads is still not well standardized it is the most cumbersome part of sequencing projects. 
We present here approaches undertaken on the way to the draft assembly the cucumber (Cucumis sativus L. cv. Borszczagowski) genome done with the use of 8x unpaired and 4x paired (3 Kbp) pyrosequenced 454 XLR Titanium reads, together BAC library ends fragments (12,7x physical coverage). Two different assembly approaches, namely Celera and Celera/Arachne were finally used. The Celera result was 15’196 contigs of 197 Mbp length and N50 27’086 bp and 4’157 scaffolds of 224 Mbp length...
Tipo: Poster Palavras-chave: Biotechnology; Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/5466/version/1
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21 Tesla Micro-MRI of Rat Skin Nature Precedings
Rakesh Sharma; Suniket Fulzele; Kiran Shetty; Mandeep Sachdeva; Bruce R. Locke.
*Aim:* A rapid ultra high resolution ex vivo MRI imaging method was evaluated to visualize rat skin structure. The main objectives were:1.qualitative assessment of viable epidermis, hair, oil and fat-rich skin features by multi-contrast approach;2.achievement of spatial resolution up to 15 microns; 3.comparison of skin histology with MRI skin features obtained by 21 Tesla magnetic resonance imaging (MRI) imager. 

*Introduction:* The ultrawide bore 900 MHz magnet has high resolution NMR magnet has a central field of 21.1 T. The high resolution MRI technique has potential for both quantitative and non-invasive morphological evaluation at micrometer level. The MRI signal was sensitive to water protons and glycolipid...
Tipo: Poster Palavras-chave: Biotechnology.
Ano: 2008 URL: http://precedings.nature.com/documents/2639/version/1
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21 Tesla MRI Microimaging of Rat Heart by Superparamagnetic Antimyoglobin Bound Nanoparticles Nature Precedings
Rakesh Sharma.
Superparamagnetic iron-oxide bound antimyoglobin (SPIOM) characteristics were analyzed at different pH. After injecting SPIOM in heart, ex vivo magnetic resonance microimaging (MRM) technique was used to visualize microvasculature of rat heart including cardiac arteries, veins, cordate tendons attached with valves. The measurement accuracy of ventricles, aorta, vasculature and dimensions of cordate tendons were close to coregistered histology measurements.
Tipo: Poster Palavras-chave: Biotechnology.
Ano: 2008 URL: http://precedings.nature.com/documents/2643/version/1
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21 Tesla MRI Microimaging of Rat Heart by Superparamagnetic Antimyoglobin Bound Nanoparticles Nature Precedings
Rakesh Sharma.
Superparamagnetic iron-oxide bound antimyoglobin (SPIOM) characteristics were analyzed at different pH. After injecting SPIOM in heart, ex vivo magnetic resonance microimaging (MRM) technique was used to visualize microvasculature of rat heart including cardiac arteries, veins, cordate tendons attached with valves. The measurement accuracy of ventricles, aorta, vasculature and dimensions of cordate tendons were close to coregistered histology measurements.
Tipo: Poster Palavras-chave: Biotechnology.
Ano: 2009 URL: http://precedings.nature.com/documents/2643/version/2
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21 Tesla MRI Microscopy of Mice Kidney, Heart and Skin: Quantitation of MRI Visible Features Nature Precedings
S Sharma; Rakesh Sharma.
Excised mice kidney, heart and skin MRI visible features were quantitated using ex vivo 21 Tesla MRI microimager. The fast MRI acquisition protocols of FLASH, gradient echo flow compensated (GEFC) techniques were used to visualize microvasculature of mice kidney, mice heart including cardiac arteries, veins, cordate tendons and mice skin epidermis with hair follicle features.
Tipo: Presentation Palavras-chave: Biotechnology.
Ano: 2009 URL: http://precedings.nature.com/documents/3484/version/1
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21 Tesla Rat Heart Magnetic Resonance Microimaging by Paramagnetic Anti-Troponin Bound Polyethylene Based Iron-Oxide Nanoparticles and Image Processing Nature Precedings
Rakesh Sharma.
21T MR microimaging with the first use of troponin nanoparticles enhances the visualization of cardiac muscle fibers and offers technical advancement for the future. Diffusion weighting offers fiber tracking and functional analysis. Image processing offers heart probabilistic atlas and maps.

http://www.ismrm.org/10/program/ELECTRONICPOSTERS.htm
Tipo: Presentation Palavras-chave: Biotechnology; Bioinformatics.
Ano: 2011 URL: http://precedings.nature.com/documents/6650/version/1
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A Bacterial Enzyme Catalyzing Double Reduction of a β,δ-Diketo Ester with Unprecedented Stereoselectivity Nature Precedings
Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2008 URL: http://precedings.nature.com/documents/1697/version/1
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A Bacterial Enzyme Catalyzing Double Reduction of a β,δ-Diketo Ester with Unprecedented Stereoselectivity Nature Precedings
Xuri Wu; Nan Liu; Yunmian He; Yijun Chen.
Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A...
Tipo: Manuscript Palavras-chave: Biotechnology; Chemistry; Microbiology.
Ano: 2010 URL: http://precedings.nature.com/documents/1697/version/2
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A biotecnologia de plantas no CNPGC: sofisticação dispensável ou inevitável? Repositório Alice
SOUZA, F. H. D. de..
bitstream/item/172195/1/A-biotecnologia-de-plantas-no-CNPGC-sofisticacao-dispensavel-ou-inevitavel.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Biotecnologia; Engenharia genética; Biotechnology.
Ano: 1989 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/321098
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A call for disruptive innovation in science publishing with a new open data-sharing platform for the life sciences Nature Precedings
Donald C. Cooper.
“A disruptive innovation is an innovation that helps create a new market and value network, and eventually goes on to disrupt an existing market and value network (over a few years or decades), displacing an earlier technology. The term is used in business and technology literature to describe innovations that improve a product or service in ways that the market does not expect.” -Wikipedia
On April 3rd, 2012 Nature Precedings, Nature Publishing Group’s experiment in free pre-print publishing was shut down and no longer accepts submissions. According to the Nature Precedings website it was created in 2007 as “a place for researchers to share documents, including presentations, posters, white...
Tipo: Manuscript Palavras-chave: Biotechnology; Neuroscience; Bioinformatics.
Ano: 2012 URL: http://precedings.nature.com/documents/7151/version/1
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A comparative study on the production of ethanol from lignocellulosic biomass by chemical and biological method Nature Precedings
Shaheed SM; Rohit JP; Madhan R; Selvakumar K.
Ethanol derived from non-edible biomass is renewable and a clean source of energy. It is independent of the food industry and it is economically feasible. The first generation biofuel or bioethanol is still not a very convenient source of energy as it prominently depends on the availability of grains. The main objective of this work is to develop an industrious efficient process to produce ethanol from lignocellulosic biomasses like wood and leaf in a lab scale. Two processes were compared. The first process involved an alkaline pre-treatment of the powdered biomass followed by dilute acid hydrolysis. The second process involved an alkaline treatment followed by direct hydrolysis of the biomass by use of a fungal species obtained from rotting wood....
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2011 URL: http://precedings.nature.com/documents/6579/version/1
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A Critical Evaluation of Clinical Trials in Cancer and Pharmacogenomics Nature Precedings
I. C. Baianu.
A critical overview of recent clinical trials in cancer is presented focused on signaling pathways blockers or inhibitors with a view to developing successful clinical trials employing personalized cancer therapies. Rational, pharmacogenomic strategies in cancer trials should be adopted that include specific molecular targeting based on adequate data for, and detailed modeling of, cancer cell genomes, modifications of cancer signaling pathways and epigenetic mechanisms. Novel translational oncogenomics research is rapidly expanding through the application of highly sensitive and specific advanced technology, research findings and computational tools and complex models to both pharmaceutical and clinical problems. Multiple sample analyses from several...
Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Chemistry; Developmental Biology; Genetics & Genomics; Immunology; Molecular Cell Biology; Pharmacology; Bioinformatics.
Ano: 2012 URL: http://precedings.nature.com/documents/7052/version/1
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A database of naturally occurring human urinary peptides and proteins for use in clinical applications Nature Precedings
Petra Zürbig; Joshua Coon; Hartwig Bauer; Georg Behrens; Mohammed Dakna; Anna Dominiczak; Stephane Decramer; Jochen Ehrich; Danilo Fliser; Moritz Frommberger; Arnold Ganser; Mark Giolami; Igor Golovko; David Good; Wilfried Gwinner; Marion Haubitz; Stefan Herget-Rosenthal; Holger Jahn; George Jerums; Bruce Julian; Markus Kellmann; Volker Kliem; Walter Kolch; Andrzej Krolewski; Mario Luppi; Ziad Massy; Michael Melter; Christian Neusüss; Jan Novak; Karlheinz Peter; Kasper Rossing; Harald Rupprecht; Joost Schanstra; Eric Schiffer; Jens-Uwe Stolzenburg; Lise Tarnow; Dan Theodorescu; Visith Thongboonkerd; Raymond Vanholder; Eva Weissinger; Harald Mischak; Philippe Schmitt-Kopplin.
Owing to its availability, ease of collection and correlation with (patho-) physiology, urine is an attractive source for clinical proteomics. However, the lack of comparable datasets from large cohorts has greatly hindered development in this field. Here we report the establishment of a high resolution proteome database of naturally occurring human urinary peptides and proteins - ranging from 800-17,000 Da - from over 3,600 individual samples using capillary electrophoresis coupled to mass spectrometry, yielding an average of 1,500 peptides per sample. All processed data were deposited in an SQL database, currently containing 5,010 relevant unique urinary peptides that serve as classifiers for diagnosis and monitoring of diseases, including kidney and...
Tipo: Manuscript Palavras-chave: Biotechnology.
Ano: 2007 URL: http://precedings.nature.com/documents/1219/version/1
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A Dynamic Decision Model of Technology Adoption under Uncertainty: Case of Herbicide-Resistant Rice AgEcon
Annou, Mamane Malam; Wailes, Eric J.; Thomsen, Michael R..
Herbicide-resistant (HR) rice technology is a potential tool for control of red rice in commercial rice production. Using an ex ante mathematical programming framework, this research presents an empirical analysis of HR rice technology adoption under uncertainty. The analysis accounts for stochastic germination of red rice and sheath blight to model a profit maximization problem of crop rotation among HR rice, regular rice, and soybeans. The results demonstrate that risk attitudes and technology efficiency determine adoption rates and optimal rotation patterns.
Tipo: Journal Article Palavras-chave: Biotechnology; Herbicide resistance; Mathematical programming; Profit maximization; Rice; Risk; Rotation; Technology; Adoption; Crop Production/Industries; Production Economics; Research and Development/Tech Change/Emerging Technologies; Q16; Q18; O33; C61.
Ano: 2005 URL: http://purl.umn.edu/43724
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A gap analysis of confined field trial application forms for genetically modified crops in East Africa: evaluating the potential for harmonization AgEcon
Linacre, Nicholas A.; Cohen, Joel I..
The regulatory approval of genetically modified crops in the field initially requires small, restricted experimental trials known as confined field trials. These small scale experiments provide researchers with important information on environmental interactions and agronomic performance of the crop in a safe and contained manner. To authorize confined field trials regulatory review is required, with formats for obtaining relevant information differing from country to country. In this paper, a Gap Analysis is used to identify informational gaps and potential for harmonization of confined field trial application processes in three East African countries - Tanzania, Kenya, and Uganda. The basic principle behind gap analysis is a comparison of the status quo...
Tipo: Report Palavras-chave: Genetically modified crops; Gap analysis; Confined field trials; Biotechnology; Biosafety; Crop Production/Industries.
Ano: 2006 URL: http://purl.umn.edu/55421
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A Meta-Analysis of Genetically Modified Food Valuation Studies AgEcon
Lusk, Jayson L.; Jamal, Mustafa; Kurlander, Lauren; Roucan, Maud; Taulman, Lesley.
A plethora of research in recent years has been devoted to estimating consumer demand for genetically modified food, an important piece of information needed to create appropriate public policy. To examine this body of work, a meta-analysis was conducted of 25 studies that, in aggregate, report 57 valuations for GM food. Findings indicate as much as 89% of the variation in existing value estimates for genetically modified food can be explained by an econometric model that controls for (a) the characteristics of the sample of consumers studied, (b) the method for eliciting consumers' valuation, and (c) characteristics of the food being valued. Each of these factors has a statistically significant effect on estimated premiums for non-GM food. Results of...
Tipo: Journal Article Palavras-chave: Biotechnology; Consumer acceptance; Genetically modified food; Willingness to pay; Consumer/Household Economics.
Ano: 2005 URL: http://purl.umn.edu/30782
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A Micropropagation, Molecular and Physiochemical Analysis of Canola Brassica napus L. From Seeds Nature Precedings
Israr Khan; Muhammad Shuaib; Sikandar Khan; Muhammad Amir Zia; Haji Akbar.
Canola is important for its oil, anticarcinogenic phytochemicals and low saturated fat content and has wide scope for further improvement through genetic transformations. Micropropagation is the practice of rapidly multiplying stock plant material to produce a large number of disease free progeny plants, using modern asceptic plant tissue culture methods and also to conserve germ plasm. A combination of auxin and cytikinin and a shock on growth regulator free medium is used for micropropagation. Molecular and physiochemical analysis done to know variation in seeds protein content and electric conductivity.
Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics; Molecular Cell Biology; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4781/version/2
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A MODEL OF PESTICIDE RESISTANCE AS A COMMON PROPERTY AND EXHAUSTIBLE RESOURCE AgEcon
Secchi, Silvia; Babcock, Bruce A..
A dynamic farm production model analyzes the interaction between the externalities caused by pest mobility and the development of pesticide resistance, a nonrenewable resource, in the context of agricultural biotechnologies. The model measures the effect of farmers' myopic behavior and the impact of pest mobility on the path of resistance.
Tipo: Conference Paper or Presentation Palavras-chave: Biotechnology; Common property resource; European Corn Borer; Externalities; Pesticide resistance; Intertemporal optimization; Research and Development/Tech Change/Emerging Technologies.
Ano: 1999 URL: http://purl.umn.edu/21664
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A new outlook towards kidney injuries Nature Precedings
Mahesh Satwekar; Abhijeet Satwekar.
Acute and chronic progression of injury to the kidney leads to the failure of the renal system and has become an increasingly important cause of morbidity and mortality. Present diagnosis detects the condition only after irreversible loss of 70 percent of kidney function. Current research is focused only on the clinical manifestations after the kidney injuries and not towards the exact cause of the condition. Here we propose a new outlook- that there is an involvement of a pathogen in the pathogenesis of kidney injuries. Basis for our proposal is given by the similarity of the pathogenesis events occurring between a classical example of hepatitis and kidney injuries. Furthermore, literature regarding the role of early kidney injury biomarkers in innate...
Tipo: Manuscript Palavras-chave: Biotechnology; Immunology; Microbiology.
Ano: 2012 URL: http://precedings.nature.com/documents/7021/version/1
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