|
|
|
| Registros recuperados: 18 | |
|
|
| Dabert, P.; Sialve, B.; Delgenès, J.P.; Moletta, R.; Godon, J.J.. |
| The microbial community of a conventionalanaerobic–aerobic sequencing batch reactor was investigated by cloning and sequencing bacterial 16S rDNA. The 92 16S rDNA sequences analysed ranged across 50 different operational taxonomic units (OTU). The majority of these sequences were not closely related to known species. They belonged to 12 different groups, but essentially to the Cytophagales and the Proteobacteria beta, which represented 38% and 17% of the retrieved sequences respectively. No OTU numerically outnumbered the others. However, similarities were observed with previous reports on molecular characterisation of phosphorus-accumulating ecosystems, suggesting an enrichment in microorganisms belonging to the Rhodocyclus group. Thereafter,... |
| Tipo: Journal Article |
Palavras-chave: TRAITEMENT DES EAUX USEES; BIOTECHNOLOGIE; SSCP; CYTOPHAGALES; PROTEOBACTERIA; RHODOCYCLUS; CARACTERISATION; REACTEUR SEQUENTIEL DISCONTINU; ANALYSE PHYLOGENETIQUE; SEQUENCAGE ADN. |
| Ano: 2001 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200032519094341&uri=/notices/prodinra1/2010/09/ |
| |
|
| |
|
| |
|
| |
|
|
| Quemener, B.; Lahaye, M.. |
| The sugar determination of the sulfated galactans, agars and carrageenans of various red algae was performed using two different techniques of depolymerisation with subsequent HPLC analysis: 1) reductive hydrolysis/ HPAEC-PAD; 2) mild methanolysis/ RPLC-DR. Both techniques were optimized to release quantitatively the composite sugars (galactose, 6-O-methyl-galactose, the labile 3,6-anhydrogalactose and 2-O-methyl-3,6-anhydrogalactose residues) and precise relative response factors of authentic 3,6-anhydrogalactose were determined. The methanolysate neutralisation step, performed subsequently to methanolysis depolymerisation, was demonstrated as a key step for the quantitative recovery of the anhydrogalactose residues. The yield of the main sugars released... |
| Tipo: Journal Article |
Palavras-chave: GALACTANE SULFATE; ALGUE ROUGE; RHODOPHYCEAE; CARACTERISATION; CHROMATOGRAPHIE HPLC; CHROMATOGRAPHIE HPLC EN PHASE INVERSE; METHANOLYSE; CHROMATOGRAPHIE D'ECHANGE D'IONS; CHROMATOGRAPHIE GAZ LIQUIDE; GALACTANE SULFATED GALACTANS; RED ALGAE; REDUCTIVE HYDROLYSIS; HIGH PERFORMANCE ANION EXCHANGE CHROMATOGRAPHY; METHANOLYSIS; REVERSE PHASE LIQUID CHROMATOGRAPHY. |
| Ano: 1998 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9800012150070225&uri=/notices/prodinra1/2010/11/ |
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Bès, C.; Briand-Longuet, L.; Cerutti, M.; Heitz, F.; Troadec, S.; Pugnière, M.; Roquet, F.; Molina, F.; Casset, F.; Bresson, D.; Péraldi-Roux, S.; Devauchelle, G.; Devaux, C.; Granier, C.; Chardès, T.. |
| We analyzed antigen-binding residues from the variable domains of anti-CD4 antibody 13B8.2 using the Spot method of parallel peptide synthesis. Sixteen amino acids, defined as Spot critical residues (SCR), were identified on the basis of a 50% decrease in CD4 binding to alanine analogs of reactive peptides. Recombinant Fab 13B8.2 mutants were constructed with alanine residues in place of each of the 16 SCR, expressed in the baculovirus cell system, and purified. CD measurements indicated that the mutated proteins were conformationally intact, with a β-sheet secondary structure similar to that of wild-type Fab. Compared with the CD4-binding capacity of wild-type Fab 13B8.2, 11 light (Y32-L, W35-L, Y36-L, H91-L, and Y92-L) and heavy chain (H35-H, R38-H,... |
| Tipo: Journal Article |
Palavras-chave: ANTICORPSMUTATION; CARACTERISATION. |
| Ano: 2003 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400025195106087&uri=/notices/prodinra1/2010/11/ |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Fleury, N.; Lahaye, M.. |
| The chemical composition of fucans isolated from leach-water, an industrial alginate extraction by-product, was investigated. Several fractions were obtained by anion exchange and gel permeation chromatography. They all contained fucose, but differed in the uronic acid, sulfate, xylose and galactose contents. They distributed as a continuum between uronic acid rich and sulfate poor to sulfate rich and uronic acid poor molecules. Two highly sulfated fractions were studied in particularly by chemical means (methylation, carboxy reduction, desulfation, controlled acid hydrolysis) and by13C nuclear magnetic resonance spectroscopy. One fraction consisted of a highly branched fucan (43.8% fucose) composed mostly of 1,2,3,4- and 1,2,4-linked fucose with some... |
| Tipo: Journal Article |
Palavras-chave: FUCANE; FUCOSE; CARACTERISATION; COMPOSITION CHIMIQUE; STRUCTURE CHIMIQUE; ALGINATE; SOUS PRODUIT ALGINATE INDUSTRIAL BY-PRODUCTS; LEACH-WATER; CHEMICAL CHARACTERIZATION; FUCANS. |
| Ano: 1993 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9400012166036970&uri=/notices/prodinra1/2010/11/ |
| |
|
| |
| Registros recuperados: 18 | |
|
|
|
