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Registros recuperados: 22
Primeira ... 12 ... Última
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A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis Genet. Mol. Biol.
Marins,Luis F.; Levy,Jose A.; Folch,Josep M.; Sanchez,Armand.
The GH (growth hormone) cDNA sequence of the marine silverside fish Odontesthes argentinensis was obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The marine silverside GH cDNA sequence is 928 nucleotides long and was found to encode a polypeptide of 204 amino acids, including a signal peptide of 17 amino acids. The 5' and 3' untranslated regions of the messenger are 109 and 204 nucleotides long, respectively. The deduced GH amino acid sequence was used to infer a phylogenetic tree with GH amino acid sequences from representative species belonging to the Euteleostei Subdivision using the maximum parsimony method. The topology found is according to the major phylogenetic grouping of euteleosts. The results corroborate the hypothesis that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth hormone; CDNA; RACE (Rapid Amplification of cDNA Ends); Phylogeny.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300013
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Análise da expressão temporal de genes relacionados ao desenvolvimento das fibras em algodoeiro. Repositório Alice
PINHEIRO, M. P. N.; BATISTA, V. G. L.; MARTINS, N. F.; MELO FILHO, P. de A.; SANTOS, R. C. dos; LIMA, L. M. de.
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: ALGODÃO; GOSSYPIUM HIRSUTUM; CDNA; FISIOLOGIA DA REPRODUÇÃO; FUNÇÃO GÊNICA.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/930730
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Brazilian coffee genome project: an EST-based genomic resource Braz. J. Plant Physiol.
Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães.
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; CDNA; EST; Transcriptome.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008
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Caracterização biológica e molecular de isolados de Apple stem grooving virus e construção de um clone infeccioso. Repositório Alice
SOUZA, E. B. de.
O Apple stem grooving virus (ASGV), espécie tipo do gênero Capillovirus, é conhecido como um vírus latente causador do acanelamento do lenho da macieira, devido ao principal sintoma observado em espécies suscetíveis, infecção podendo levar à perda de qualidade de frutos e queda na produtividade. O vírus já foi diagnosticado em diversas partes do mundo e atualmente, existem depositados no GenBank, dezoito sequências de genomas completos de isolados de ASGV, e várias sequências parciais principalmente da região da capa proteica. O presente trabalho está dividido em dois capítulos. O capítulo l teve como objetivo a caracterização biológica e molecular de dois isolados de ASGV (M219-3 e M220), e de forma mais específica, a clonagem e sequenciamento do genoma...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Malus domestica; ASGV-sequenciamento; Capillovirus Betaflexiviridae; Gibson Assembly; CDNA.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1060658
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Caracterização de blibliotecas substrativas de cDNA de genótipos suscetível e resistente de algodão (Gossypium hirsutum) infectados com Meloidogyne incognita. Repositório Alice
ROCHA, S. R.; PAES, N. S.; SALES, R. M. O. B.; SILVA, F. R.; GURGEL, F. L.; CARNEIRO, R. M. D.; GROSSI-DE-SÁ, M. F.; MEHTA, A..
2006
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Biologia molecular; Algodão; Gossypium hirsutum; CDNA; Genótipo suscetível; Genótipo resistentes.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/188530
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cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake BJMBR
Fagundes,F.H.R.; Oliveira,M.; Huancahuire-Vega,S.; Romero-Vargas,F.F.; Ponce-Soto,L.A.; Marangoni,S..
To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR), we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49), from the venom of Crotalus durissus collilineatus (Cdc PLA2). The cDNA encoding Cdc PLA2 from whole venom was sequenced. The deduced amino acid sequence of this cDNA has high overall sequence identity with the group II PLA2 protein family. Cdc PLA2 has 14 cysteine residues capable of forming seven disulfide bonds that characterize this group of PLA2 enzymes. Cdc PLA2 was isolated using conventional Sephadex G75 column chromatography and reverse-phase high performance liquid chromatography (RP-HPLC)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cdc PLA2; CDNA; Chick biventer cervicis; Crotalus durissus collilineatus; Mass spectrometry MALDI-TOF; Neurotoxic agents.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300007
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Código de barras de ADN de las especies cubanas de peces dulceacuícolas. OceanDocs
Lara Lorenzo, A..
En el presente trabajo se realizó el análisis del código de barras ADN de 24 especies de la ictiofauna dulceacuícola cubana. Para ello, se secuenciaron 652pb del gen mitocondrial citocromo oxidasa I (COI) de 96 individuos provenientes de diferentes áreas geográficas y representativos, en la mayoría de los casos, de la distribución espacial de cada especie. En el análisis se utilizó la distancia genética Kimura 2 parámetros (K2P), considerando como cota superior el valor de 3%, para identificar los límites de divergencia entre especies. Complementariamente se llevó a cabo un análisis de agregación poblacional para identificar grupos poblacionales indicativos de linajes evolutivos independientes. El estimado promedio de divergencia genética entre...
Tipo: Theses and Dissertations Palavras-chave: CDNA; Genes; Fishes; Fish wastes.
Ano: 2008 URL: http://hdl.handle.net/1834/5514
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Decifrando o genoma. Repositório Alice
CARNEIRO, N. P.; CARNEIRO, A. A.; GUIMARAES, C. T.; PAIVA, E..
Os genes, unidades biologicas que determinam as caracteristicas de um organismo, estao contidos em moleculas de DNA presentes no nucleo das celulas. Portanto, o estudo do DNA e de fundamental importancia para o entendimento de como as caracteristicas de um organismo sao formadas. Com o grande avanco alcancado pelas tecnicas de Biologia molecular nas ultimas decadas, hoje, somos capazes de isolar e sequenciar moleculas de DNA de maneira rotineira. A partir da otimizacao das tecnicas de sequenciamento, surgiram programas com o objetivo de sequenciar genomas inteiros. atualmente, estao depositados em bancos de dados mais de 3 milhoes de sequencias de DNA e, grande parte delas ainda nao tem sua funcao conhecida. O processo de caracterizacao da funcao genica...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genoma; Sequenciamento; CDNA; Genome; DNA sequency.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484108
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Evaluation of cellulolytic filamentous fungi phenotypes using randomly amplified cDNA with RAPD primers. Repositório Alice
OLIVEIRA, E. M. M.; BARROS, N. E. F.; FERREIRA, T. S.; OLIVEIRA, T. C.; TERZI, S. C.; DAMASO, M. C. T..
2010
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Fungo filamentoso; Aspergillus niger; Enzima; CDNA; RAPD.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/878024
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Gene expression: a review on methods for the study of defense-related gene differential expression in plants. Repositório Alice
CASASSOLA, A.; BRAMMER, S. P.; CHAVES, M. S.; MARTINELLI, J. A.; GRANDO, M. F.; DENARDIN, N. D..
2013
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Transcriptomics; CDNA; Metabolic Routes; Plant Defense; Gene Expression; Molecular Biology.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/981342
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Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis) Genet. Mol. Biol.
Naskar,Soumen; Deb,Sitangsu M.; Niranjan,Saket K.; Kumar,Subodh; Sharma,Deepak; Sakaram,Durgam; Sharma,Arjava.
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water buffalo; CDNA; DRB; MHC; Phylogeny.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013
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Molecular cloning, expression and characterization of a serine proteinase from Japanese edible mushroom, Grifola frondosa: solving the structure - function anomaly of a reported aminopeptidase Electron. J. Biotechnol.
Islam,Mohammed M.
The N-terminal amino acid sequence of an aminopeptidase from Japanese edible mushroom, Grifola frondosa, was reported to have high similarity with that of a serine proteinase from basidiomycete, Agaricus bisporous (Nishiwaki and Hayashi, 2001). The full-length cDNA and the corresponding genomic DNA of the enzyme were cloned, based on the reported N-terminal amino acid sequence. The predicted open reading frame (ORF) of the cloned cDNA, encoding a product of 379 amino acids, was expressed in E. coli using pET expression vector. The expressed pro-enzyme (40 kDa) underwent autolysis to produce the mature protein (30 kDa) and a pro-peptide (10 kDa). The mature protein and the pro-peptide remained tightly bound to each other and could not be separated by Ni-NTA...
Tipo: Journal article Palavras-chave: 5'-RACE; CDNA; Genomic DNA; Maitake; Refolding.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400010
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Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits Genet. Mol. Biol.
Sammour,Reda H..
The genes encoding the glycinin subunits G2 and G4 were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. The recombinant DNAs pSP65/G2HG4, pSP65/G4HG2, pSP65/248 Metl, pSP65/248 Met2,3 and pSP65/248 Metl.2,3 were used in in vitro translation to produce (i) chimeric proteins consisting of reciprocally exchanged acidic and basic G2 and G4 domains and (ii) Gy4 point mutants with an increased number of methionine residues. The ability of the recombinant proteins to assemble into proper quaternary structures was investigated using sucrose gradient fractionation. The data produced by this study could provide valuable clues for the potential improvement of genetically modified crops.
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDNA; Glycinin subunit G4; In vitro translation; Mutagenesis; Soybean.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300025
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Possible involvement of CsTypA1 in reproductive organ development of cucumber (Cucumis sativus) National Institute of Agronomic Research
Barak, M.; Trebitsh, T..
Cucumber (Cucumis sativus) is a monoecious plant that is extensively studied with respect to floral sex determination. While the genetic background, hormonal and environmental factors are well characterized, the molecular mechanisms regulating unisexual flower development are not well understood. Initially a cucumber floral bud is bisexual and has the potential to develop into a male or female flower. Using differential cDNA-AFLP analysis between plant apices of monoecious (predominantly male) and gynoecious (female) cucumber plants, we isolated a cDNA that encodes a putative GTP binding protein tyrosine phosphorylated protein A (CsTypA1). TypA genes are widely distributed in plants and prokaryotes. Bacterial TypA proteins are involved in protein...
Tipo: Poster Palavras-chave: Flower development; Floral sex; Ovary; Cucumis sativus; Cucumber; CDNA; Sex determination.
Ano: 2008 URL: http://hdl.handle.net/2174/282
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RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct Biocell
Valdecantos,Pablo A.; Argañaraz,Martín E.; Abate,Carlos M.; Miceli,Dora C..
As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this sequence hybridizes with 2.9 kb and 4.1 kb mRNAs in early pregnant rat oviducts. However,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential gene expression; Oviduct; RNA fingerprint; CDNA.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300005
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Sequence analysis of the cDNA encoding for SpCTx: a lethal factor from scorpionfish venom ( Scorpaena plumieri ) J. Venom. Anim. Toxins incl. Trop. Dis.
Costa,Fábio L. S.; Lima,Maria Elena De; Figueiredo,Suely G.; Ferreira,Rafaela S.; Prates,Núbia S.; Sakamoto,Tetsu; Salas,Carlos E..
Abstract Background: Lethal factors are multifunctional oligomeric proteins found in the venomous apparatus of Scorpaeniformes fish. These toxins elicit not only an array of biological responses in vitro but also cardiovascular disorders and strong hemolytic, nociceptive and edematogenic activities in vivo. This work describes the cloning and molecular identification of two toxin subunits, denominated Sp-CTx-α and Sp-CTx-β, from scorpionfish venom ( Scorpaena plumieri ). Methods: The primary structures were deduced after cDNA amplification by PCR with primers from conserved sequences described in Scorpaeniformes toxins. Following DNA sequencing and bioinformatic analysis, the tridimensional structures of both subunits were modeled. Results: The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDNA; Lethal factor; Scorpaena plumieri; Scorpionfish; Venom gland.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100315
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Sequence Analysis of Three Major Antigens (P30, P23 and P22) of Virulent and Avirulent Strains of Toxoplasma gondii OAK
Maki, Yoshiyuki; Seng, Seyha; Kato, Mihoko; Hoshi, Yukihiro; Igarashi, Ikuo; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma gondii; SAG-1 (P30); CDNA.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/250
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Suppression subtractive hybridization PCR isolation of cDNAs from a Caribbean soft coral Electron. J. Biotechnol.
Lopez,Jose V; Ledger,Angela; Santiago-Vázquez,Lory Z; Pop,Mihai; Sommer,Dan D; Ranzer,Llanie K; Feldman,Robert A; Russell,G. Kerr.
Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non-“housekeeping” genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila...
Tipo: Journal article Palavras-chave: CDNA; Erythropodium caribaeorum; EST; Gorgonia ventalina; Gorgonian; Sea fan.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100008
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The lift pool method for isolation of cDNA clones from lambda phage libraries Electron. J. Biotechnol.
LeBlanc-Straceski,Janine; Sobrado,Pablo; Betz,Sharon; Zerfas,Julie; Morgan,Karen.
A PCR based strategy was developed to screen a Xenopus oocyte λgt10 cDNA library. The PCR-based lift pool (LP) method follows the same two tiered strategy as conventional screening of phage libraries by filter hybridization. Two rounds of plating, one at high density to detect the clone, and one at low density to purify the clone to homogeneity, are performed. In the first round, lysates from high density plates, termed plate pools (PP), serve as template for PCR. In the second round, phage particles adhering to plaque lifts of low density plates are washed off nitrocellulose filters to create LPs, which are used as template for PCR. The integrity of the plaques on the low-density plates is preserved. Once a positive LP has been identified,...
Tipo: Journal article Palavras-chave: CDNA; Lambda; Non-radioactive; PCR; Plaque; Screening.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400012
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Three synonymous genes encode calmodulin in a reptile, the Japanese tortoise, Clemmys japonica Genet. Mol. Biol.
Shimoda,Kouji; Miyake,Toshihiro; Kimura,Jun; Maejima,Kazuyoshi.
Three distinct calmodulin (CaM)-encoding cDNAs were isolated from a reptile, the Japanese tortoise (Clemmys japonica), based on degenerative primer PCR. Because of synonymous codon usages, the deduced amino acid (aa) sequences were exactly the same in all three genes and identical to the aa sequence of vertebrate CaM. The three cDNAs, referred to as CaM-A, -B, and -C, seemed to belong to the same type as CaMI, CaMII, and CaMIII, respectively, based on their sequence identity with those of the mammalian cDNAs and the glutamate codon biases. Northern blot analysis detected CaM-A and -B as bands corresponding to 1.8 kb, with the most abundant levels in the brain and testis, while CaM-C was detected most abundantly in the brain as bands of 1.4 and 2.0 kb. Our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reptile; CDNA; Synonymousgenes.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100009
Registros recuperados: 22
Primeira ... 12 ... Última
 

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