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Registros recuperados: 7
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Cloning and expression pattern of a putative octopamine/tyramine receptor in antennae of the noctuid moth Mamestra brassicae Inra
Brigaud, I.; Grosmaître, X.; Francois, M.C.; Jacquin-Joly, E..
In insects, biogenic amines have been shown to play an important role in olfactory plasticity. In a first attempt to decipher the underlying molecular mechanisms, we report the molecular cloning and precise expression pattern of a newly identified octopamine/tyramine-receptor-encoding gene in the antennae of the noctuid moth Mamestra brassicae (MbraOAR/TAR). A full-length cDNA has been obtained through homology cloning in combination with rapid amplification of cDNA ends/polymerase chain reaction; the deduced protein exhibits high identities with previously identified octopamine/tyramine receptors in other moths. In situ hybridization within the antennae has revealed that MbraOAR/TAR is expressed at the bases of both pheromone-sensitive and non-sensitive...
Tipo: Journal Article Palavras-chave: CLONAGE; LOBE ANTENNAIRE; NOCTUIDAE; MITE; HEXAPODA; ARTHROPODA; AMINE BIOGENIQUE; RECEPTEUR; CLONAGE MOLECULAIRE; ADNC; POLYMORPHISME; PCR; PHEROMONE; LEPIDOPTERA; MAMESTRA BRASSICAE OCTOPAMINE/TYRAMINE RECEPTOR; EXPRESSION PATTERN; OLFACTION; OLFACTORY PLASTICITY.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201084fc8d7c&uri=/notices/prodinra1/2010/11/
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Cloning of an octopamine/tyramine receptor and plasticity of its expression as a function of adult sexual maturation in the male moth Agrotis ipsilon Inra
Duportets, L.; Barrozo, R.B.; Bozzolan, F.; Gaertner, C.; Anton, S.; Gadenne, C.; Debernard, S..
In the male moth Agrotis ipsilon behavioural response and antennal lobe (AL) neuron sensitivity to the female-produced sex pheromone increase with age and juvenile hormone (JH) level. We recently showed that the neuromodulator, octopamine (OA), interacts with JH in this age-dependent olfactory plasticity. To further elucidate its role, we cloned a full cDNA encoding a protein that presents biochemical features essential to OA/tyramine receptor (AipsOAR/TAR) function. The AipsOAR/TAR transcript was detected predominantly in the antennae, the brain and, more specifically, in ALs where its expression level varied concomitantly with age. This expression plasticity indicates that AipsOAR/TAR might be involved in central processing of the pheromone signal during...
Tipo: Journal Article Palavras-chave: LOBE ANTENNAIRE; LEPIDOPTERA; NOCTUIDAE; AGROPIS IPSILON; RECEPTEUR; OLFACTION; MITE; INSECTE; MATURATION SEXUELLE; ARTHROPODA; HEXAPODA; NEURONE; PHEROMONE; HORMONE; ADNC; CLONAGE; COMPORTEMENT SEXUEL; ATTRACTIF SEXUEL ANTENNAL LOBE; OCTOPAMINE/TYRAMINE RECEPTOR; OLFACTORY PROCESSING; MATURATION; EXPRESSION PLASTICITY.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102d18e75d&uri=/notices/prodinra1/2011/02/
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Etude du génome des densovirus de lépidoptères ravageurs de cultures : Casphalia extranea en Côte d'Ivoire et Diatraea saccharalis au Brésil, application au diagnostic par sonde nucléique IRD
Kouassi, Koffi Nazaire.
Nous avons analysé le génome des densovirus de #C. extranea$ (#Ce$DNV) et de #D. saccharalis$ (#Ds$DNV). Les tailles de ces génomes ont été estimées à 4,9 kb pour le Ce DNV et à 5,95 kb pour celui du #Ds$DNV. Vingt sites de restriction ont été cartographiés sur le génome du #Ce$DNV et 41 sites sur celui du #Ds$DNV. La séquence du génome du #Ce$DNV correspondant aux fragments #Hind$III A et D (approx. 50 % du génome) a été clonée dans le plasmide pEMBL 19+. Le plasmide correspondant, pCeHindIIIA+D, nous a servi à la mise au point d'une sonde nucléique. La séquence quasi-complète du génome du Ds DNV a été clonée dans le même vecteur générant le plasmide pDst50. Ce plasmide transfecté dans la lignée cellulaire Px de #Plutella xylostella$ a conduit à une...
Tipo: Text Palavras-chave: LEPIDOPTERE RAVAGEUR; VIRUS ENTOMOPATHOGENE; ADN; BIOLOGIE MOLECULAIRE; DIAGNOSTIC; VIROSE; LUTTE BIOLOGIQUE; DENSIVIRUS; CLONAGE; GENOME; CULTURE CELLULAIRE; SONDE NUCLEIQUE.
Ano: 1993 URL: http://www.documentation.ird.fr/hor/fdi:38330
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Functional annotation, genome organization and phylogeny of the grapevine (Vitis vinifera) terpene synthase gene family based on genome assembly, FLcDNA cloning, and enzyme assays Inra
Martin, D.M.; Aubourg, S.; Schouwey, M.B.; Daviet, L.; Schalk, M.; Toub, O.; Lund, S.T.; Bohlmann, J..
Background: Terpenoids are among the most important constituents of grape flavour and wine bouquet, and serve as useful metabolite markers in viticulture and enology. Based on the initial 8-fold sequencing of a nearly homozygous Pinot noir inbred line, 89 putative terpenoid synthase genes (VvTPS) were predicted by in silico analysis of the grapevine (Vitis vinifera) genome assembly [1]. The finding of this very large VvTPS family, combined with the importance of terpenoid metabolism for the organoleptic properties of grapevine berries and finished wines, prompted a detailed examination of this gene family at the genomic level as well as an investigation into VvTPS biochemical functions.Results: We present findings from the analysis of the up-dated 12-fold...
Tipo: Journal Article Palavras-chave: CHROMATOGRAPHIE; SPECTROMETRIE DE MASSE; CLONAGE; ADNC; METABOLISME SECONDAIRE; PHYLOGENIE; ENZYME; TERPENOIDE; FLAVEUR; ODEUR; CARTOGRAPHIE; PROPRIETE ORGANOLEPTIQUE ENT-COPALYL DIPHOSPHATE; CHROMATOGRAPHY-MASS SPECTROMETRY; SESQUITERPENE SYNTHASES (-)-GERMACRENE-D SYNTHASE SECONDARY METABOLISM ARABIDOPSIS-THALIANA; LIMONENE SYNTHASE ESCHERICHIA-COLI; RESISTANCE GENES; CDNA CLONING.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011fadf0898&uri=/notices/prodinra1/2011/01/
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Mise au point sur le clonage des gènes, la préparation et l'utilisation des sondes d'ADN IRD
Monteny, Nicole.
Tipo: Text Palavras-chave: GENIE GENETIQUE; METHODOLOGIE; SYNTHESE; ENZYME DE RESTRICTION; ADN RECOMBINANT; PLASMIDE; COSMIDE; PHAGE; CLONAGE; SONDE.
Ano: 1987 URL: http://www.documentation.ird.fr/hor/fdi:27446
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Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis Inra
Minic, Z.; Do, C.T.; Rihouey, C.; Morin, H.; Lerouge, P.; Jouanin, L..
Tipo: Journal Article Palavras-chave: PURIFICATION; CLONAGE; IDENTIFICATION; MUTANT; HYDROLASE ARABIDOPSIS THALIANA.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e9b8fd24&uri=/notices/prodinra1/2010/11/
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The 20-hydroxyecdysone-induced signalling pathway in G2/M arrest of Plodia interpunctella imaginal wing cells Inra
Siaussat, D.; Bozzolan, F.; Porcheron, P.; Debernard, S..
The mechanisms involved in the control of cellular proliferation by the steroid hormone 20-hydroxyecdysone (20E) in insects are not known. We dissected the 20E signalling pathway responsible for G2/M arrest of imaginal cells from the IAL-PID2 cells of the Indian meal moth Plodia interpunctella. We first used a 5′–3′ RACE-based strategy to clone a 4479 bp cDNA encoding a putative P. interpunctella HR3 transcription factor named PiHR3. The deduced amino acid sequence of PiHR3 was highly similar to those of HR3 proteins from other lepidopterans, e.g. Manduca sexta and Bombyx mori. Using double-stranded RNA-mediated interference (dsRNAi), we then succeeded in blocking the ability of 20E to induce the expression of PiEcR-B1, PiUSP-2 and PiHR3 genes that encode...
Tipo: Journal Article Palavras-chave: PLODIA INTERPUNCTELLA; EUCARYOTE; ARTHROPODA; HEXAPODA; INSECTE; LEPIDOPTERA; HORMONE STEROIDE; MITE; CLONAGE; ADNC; EXPRESSION DES GENES; TRANSCRIPTION; SEQUENCE NUCLEOTIDIQUE; ACIDE AMINE  RNA INTERFERENCE; STEROID HORMONE RECEPTOR SUPERFAMILY; G2/M ARREST; B CYCLIN; SIGNALLING PATHWAY; IMAGINAL CELLS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20103c688c9f&uri=/notices/prodinra1/2010/10/
Registros recuperados: 7
Primeira ... 1 ... Última
 

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