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Registros recuperados: 10
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A taxonomic review of Penicillium species producing conidiophores with solitary phialides, classified in section Torulomyces Naturalis
Visagie, C.M.; Houbraken, J.; Dijksterhuis, J.; Seifert, K.A.; Jacobs, K.; Samson, R.A..
The genus Torulomyces was characterised by species that typically have conidiophores consisting of solitary phialides that produce long chains of conidia connected by disjunctors. Based on the phylogenetic position of P. lagena (generic ex-neotype), the genus and its seven species were transferred to Penicillium and classified in sect. Torulomyces along with P. cryptum and P. lassenii. The aim of this study was to review the species currently classified in sect. Torulomyces using morphology and phylogenies of the ITS, BenA, CaM and RPB2 regions. Based on our results, we accept 16 species in sect. Torulomyces, including 12 new species described as P. aeris, P. austricola, P. cantabricum, P. catalonicum, P. oregonense, P. marthae-christenseniae, P....
Tipo: Article / Letter to the editor Palavras-chave: Beta-tubulin; Calmodulin; Eupenicillium; Internal transcribed spacer rDNA region; Low temperature Scanning Electron Microscopy (cryo-SEM); Monocillium; RNA polymerase II second largest subunit; Trichocomaceae; Wollemi pine.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/607363
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CaMKIIdelta overexpression in hypertrophy and heart failure: cellular consequences for excitation-contraction coupling BJMBR
Maier,L.S..
Ca/calmodulin-dependent protein kinase IIdelta (CaMKIIdelta) is the predominant isoform in the heart. During excitation-contraction coupling (ECC) CaMKII phosphorylates several Ca-handling proteins including ryanodine receptors (RyR), phospholamban, and L-type Ca channels. CaMKII expression and activity have been shown to correlate positively with impaired ejection fraction in the myocardium of patients with heart failure and CaMKII has been proposed to be a possible compensatory mechanism to keep hearts from complete failure. However, in addition to these acute effects on ECC, CaMKII was shown to be involved in hypertrophic signaling, termed excitation-transcription coupling (ETC). Thus, animal models have shown that overexpression of nuclear isoform...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium; Calmodulin; CaM kinase; Excitation-contraction coupling; Heart.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900002
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Connexin domains relevant to the chemical gating of gap junction channels BJMBR
Most cells exchange ions and small metabolites via gap junction channels. These channels are made of two hemichannels (connexons), each formed by the radial arrangement of six connexin (Cx) proteins. Connexins span the bilayer four times (M1-M4) and have both amino- and carboxy-termini (NT, CT) at the cytoplasmic side of the membrane, forming two extracellular loops (E1, E2) and one inner (IL) loop. The channels are regulated by gates that close with cytosolic acidification (e.g., CO2 treatment) or increased calcium concentration, possibly via calmodulin activation. Although gap junction regulation is still unclear, connexin domains involved in gating are being defined. We have recently focused on the CO2 gating sensitivity of Cx32, Cx38 and various...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cell-cell interaction; Cell junctions; Gap junctions; Calcium; Calmodulin; Membrane channels.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500003
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Evidence for a modulation of neutral trehalase activity by Ca2+ and cAMP signaling pathways in Saccharomyces cerevisiae BJMBR
Souza,A.C.; De Mesquita,J.F.; Panek,A.D.; Silva,J.T.; Paschoalin,V.M.F..
Saccharomyces cerevisiae neutral trehalase (encoded by NTH1) is regulated by cAMP-dependent protein kinase (PKA) and by an endogenous modulator protein. A yeast strain with knockouts of CMK1 and CMK2 genes (cmk1cmk2) and its isogenic control (CMK1CMK2) were used to investigate the role of CaM kinase II in the in vitro activation of neutral trehalase during growth on glucose. In the exponential growth phase, cmk1cmk2 cells exhibited basal trehalase activity and an activation ratio by PKA very similar to that found in CMK1CMK2 cells. At diauxie, even though both cells presented comparable basal trehalase activities, cmk1cmk2 cells showed reduced activation by PKA and lower total trehalase activity when compared to CMK1CMK2 cells. To determine if CaM kinase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Trehalase; Calmodulin; CaM kinase II; CAMP-dependent protein kinase; YLR270W.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100002
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Expression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton Braz. J. Plant Physiol.
Nepomuceno,Alexandre Lima; Oosterhuis,Derrick; Stewart,James McD; Turley,Rickie; Neumaier,Norman; Farias,José Renato Bouças.
Tolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calmodulin; Drought; Gene expression; Gossypium hirsutum L.; Trehalase.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100002
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Fifteen new species of Penicillium Naturalis
Visagie, C.M.; Renaud, J.B.; Burgess, K.M.N.; Malloch, D.W.; Clark, D.; Ketch, L.; Urb, M.; Louis-Seize, G.; Assabgui, R.; Sumarah, M.W.; Seifert, K.A..
We introduce 15 new species of Penicillium isolated from a diverse range of locations, including Canada, Costa Rica, Germany, Italy, New Zealand, Tanzania, USA and the Dry Valleys of Antarctica, from a variety of habitats, including leaf surfaces in tropical rain forests, soil eaten by chimpanzees, infrabuccal pockets of carpenter ants, intestinal contents of caterpillars and soil. The new species are classified in sections Aspergilloides (1), Canescentia (2), Charlesia (1), Exilicaulis (3), Lanata-Divaricata (7) and Stolkia (1). Each is characterised and described using classical morphology, LC-MS based extrolite analyses and multigene phylogenies based on ITS, BenA and CaM. Significant extrolites detected include andrastin, pulvilloric acid, penitrem A...
Tipo: Article / Letter to the editor Palavras-chave: Beta-tubulin; Calmodulin; Internal transcribed spacer rDNA region; Trichocomaceae.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/607849
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Minimum free energy predicted base pairing in the 39 nt spliced leader and 5’ UTR of calmodulin mRNA from Trypanosoma cruzi: influence of the multiple trans-splicing sites Anais da ABC (AABC)
SAMUDIO,FRANKLYN; BRANDÃO,ADEILTON.
ABSTRACT We analyzed the compositional changes and the stable base pairs in the predicted secondary structure of the 5’ UTR calmodulin mRNA in T. cruzi. The three copies of calmodulin in T. cruzi genome display variable position of the trans splicing sites and give rise to several mRNA that differs slightly on 5’ UTR composition in the epimastigote stage. We show that the pattern of high probability base pairs in the minimum free energy predicted secondary structures of the calmodulin 5’ UTR remains unchanged despite the nucleotide composition variation. However, the 39 nt spliced leader (mini-exon, the 5’ exon sequence transferred to trypanosome mRNAs by the mechanism of trans splicing) shows a variable pattern of high and low probability base pairing as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trans-splicing; Untranslated region; Calmodulin; Epimastigote; RNA secondary structure; Minimum free energy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502311
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Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers Biol. Res.
SCHNEIDER,MARTIN F; RODNEY,GEORGE G.
Local discrete elevations in myoplasmic Ca2+ (Ca2+ sparks) arise from the opening of a small group of RyRs. Summation of a large number of Ca2+ sparks gives rise to the whole cell Ca2+ transient necessary for muscle contraction. Unlike sarcoplasmic reticulum vesicle preparations and isolated single channels in artificial membranes, the study of Ca2+ sparks provides a means to understand the regulation of a small group of RyRs in the environment of a functionally intact triad and in the presence of endogenous regulatory proteins. To gain insight into the mechanisms that regulate the gating of RyRs we have utilized laser scanning confocal microscopy to measure Ca2+ sparks in permeabilized frog skeletal muscle fibers. This review summarizes our recent studies...
Tipo: Journal article Palavras-chave: Excitation-Contraction Coupling; Ca2+ Sparks; IperatoxinA; DP4; Calmodulin.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400016
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Rat vas deferens SERCA2 is modulated by Ca2+/calmodulin protein kinase II-mediated phosphorylation BJMBR
Rodriguez,J.B.R.; Muzi-Filho,H.; Valverde,R.H.F.; Quintas,L.E.M.; Noel,F.; Einicker-Lamas,M.; Cunha,V.M.N..
Ca2+ pumps are important players in smooth muscle contraction. Nevertheless, little information is available about these pumps in the vas deferens. We have determined which subtype of sarco(endo)plasmic reticulum Ca2+-ATPase isoform (SERCA) is expressed in rat vas deferens (RVD) and its modulation by calmodulin (CaM)-dependent mechanisms. The thapsigargin-sensitive Ca2+-ATPase from a membrane fraction containing the highest SERCA levels in the RVD homogenate has the same molecular mass (∼115 kDa) as that of SERCA2 from the rat cerebellum. It has a very high affinity for Ca2+ (Ca0.5 = 780 nM) and a low sensitivity to vanadate (IC50 = 41 µM). These facts indicate that SERCA2 is present in the RVD. Immunoblotting for CaM and Ca2+/calmodulin-dependent protein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ca2+/calmodulin-dependent kinase II; Calcium homeostasis; Calmodulin; Rat vas deferens; SERCA2; Thapsigargin.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300227
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Targeting and translocation of endothelial nitric oxide synthase BJMBR
Michel,T..
This review explores advances in our understanding of the intracellular regulation of the endothelial isoform of nitric oxide synthase (eNOS) in the context of its dynamically regulated subcellular targeting. Nitric oxide (NO) is a labile molecule, and may play important biological roles both within the cell in which it is synthesized and in its interactions with nearby cells and molecules. The localization of eNOS within the cell importantly influences the biological role and chemical fate of the NO produced by the enzyme. eNOS, a Ca2+/calmodulin-dependent enzyme, is subject to a complex pattern of intracellular regulation, including co- and post-translational modifications and interactions with other proteins and ligands. In endothelial cells and cardiac...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caveolin; Signal transduction; Nitric oxide synthase; Calmodulin; Estrogen; Bradykinin.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100006
Registros recuperados: 10
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