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A method for fast determination of epigallocatechin gallate (EGCG), epicatechin (EC), catechin (C) and caffeine (CAF) in green tea using HPLC Ciênc. Tecnol. Aliment.
Saito,Samuel T.; Welzel,Albert; Suyenaga,Edna S.; Bueno,Francie.
Tea has been considered a medicine and a healthy beverage since ancient times, but recently it has received a great deal of attention because of its antioxidant properties. Green tea polyphenols have demonstrated to be an effective chemopreventive agent. Recently, investigators have found that EGCG, one of the green tea catechins, could have anti-HIV effects when bound to CD4 receptor. Many factors can constitute important influences on the composition of tea, such as species, season, age of the leaf, climate, and horticultural practices (soil, water, minerals, fertilizers). This paper presents an HPLC analytical methodology development, using column RP-18 and mobile phase composed by water, acetonitrile, methanol, ethyl acetate, glacial acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; EGCG; Catechin; Camellia sinensis; Brazilian green tea.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000200023
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Caffeine in teas: levels, transference to infusion and estimated intake Ciênc. Tecnol. Aliment.
TFOUNI,Silvia Amelia Verdiani; CAMARA,Maíra Marcuci; KAMIKATA,Kamille; GOMES,Fernanda Moralez Leme; FURLANI,Regina Prado Zanes.
Abstract Caffeine is naturally present in several foods, being one of the most consumed dietary ingredients in the world; however, excessive intake may cause health concerns. This study evaluated caffeine levels in teas and their infusions, the transference rate during brewing, and estimated caffeine intake from tea infusion. Brands and batches of 4 types of teas were analyzed for caffeine content by high performance liquid chromatography with a diode array detector. Mate tea was the one that presented lowest levels (6.1 to 13.2 mg/g) while Camellia sinensis teas were from 14.3 to 34.8 mg/g. There were statistical differences between different types, brands and batches. Caffeine levels in infusions followed the same pattern of the leaves, with mate tea...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xanthine alkaloid; Mate tea; Camellia sinensis; HPLC; Brew.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000400661
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Chá: aspectos relacionados à qualidade e perspectivas Ciência Rural
Lima,Juliana Domingues; Mazzafera,Paulo; Moraes,Wilson da Silva; Silva,Reginaldo Barboza da.
O chá preto é uma das bebidas mais consumidas no mundo. Essa bebida é feita a partir da infusão de folhas processadas de Camellia sinensis, que é cultivada em mais de 30 países, tendo grande importância socioeconômica. Estudos sugerem que o chá tem efeito protetor contra diversos tipos de câncer e doenças cardiovasculares devido à presença de polifenóis denominados catequinas, que são oxidadas enzimaticamente durante o processamento das folhas, gerando uma mistura constituída principalmente de teaflavinas, teasinensinas e tearubiginas. A produção mundial de chá tem aumentado mais do que o consumo, provocando redução do preço que, juntamente com o aumento no custo de produção, implica a necessidade de alta produtividade e qualidade. Apesar disso, ainda não...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camellia sinensis; Catequinas; Chá verde; Chá preto; Práticas agronômicas.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000400049
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Changes in phenolics and antioxidant capacity during short storage of ready-to-drink green tea (Camellia sinensis) beverage at commercial conditions Bragantia
Urías-Orona,Vania; Niño-Medina,Guillermo.
ABSTRACT The objective of this research was to evaluate the changes of phenolics and antioxidant capacity of a ready-to-drinkgreen tea beverage during short storage at commercial conditions. The total phenols (Folin-Ciocalteu), total catechins (4-dimethylaminocinnamaldehyde) and total non-catechins (difference between total phenols and total catechins) were evaluated as part of phenolic analysis, while antioxidant capacity was evaluated by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. At the beginning of the experiment, the levels of total phenols, total catechins and total non-catechins were 1220.69 ± 29.73, 636.94 ± 14.60and 584.75 ± 15.12 milligrams of catechin per liter of sample, respectively; these values decreased to 674.38 ± 26.52, 424.54 ±...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camellia sinensis; Total phenolics; Total catechins; Total non-catechins; DPPH antioxidant capacity; Short storage.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000100141
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Comparison of total phenol content and antioxidant activity of herbal infusions with added Stevia reabaudiana Bertoni Ciênc. Tecnol. Aliment.
CASTAÑEDA-SAUCEDO,Ma Claudia; RAMÍREZ-ANAYA,Jessica del Pilar; TAPIA-CAMPOS,Ernesto; DIAZ-OCHOA,Elvia Guadalupe.
Abstract Antioxidant capacity (AC) was determined by the ABTS method and DPPH, and total phenol content (TPC) in dehydrated plant material, in infusions and in residues (plant material after preparing the infusion) of white, black, red, green, spearmint, stevia, lemon grass and chamomile teas to which stevia leaves were added or not added; addition of processed stevia powder was also tested. Three independent experiments were set up: with dehydrated plant material, with infusions and with residues. For the case of dehydrated plant material, white tea had the highest TPC (10813.5 mg GAE/100g) and AC by the ABTS method (1183.3 µM TE/g) and DPPH method (1525.0 µM TE/g). In infusions, black tea had higher TPC (180.82 µg GAE/ml) and AC by the methods ABTS and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camellia sinensis; Matricaria chamomilla; Cymbopogon citratus and Mentha spicata.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100117
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Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: A comparative study with green tea (Camellia sinensis) IPB - Escola Superior Agrária
Costa, R.M.; Magalhães, A.S.; Pereira, J.A.; Andrade, P.B.; Valentão, P.; Carvalho, M.; Silva, B.M..
This study aimed to determine the phenolic profile and to investigate the antioxidant potential of quince (Cydonia oblonga) leaf, comparing it with green tea (Camellia sinensis). For these purposes, methanolic extracts were prepared and phenolics content of quince leaf was determined by HPLC/UV. The antioxidant properties were assessed by Folin–Ciocalteu reducing capacity assay and by the ability to quench the stable free radical 2,20-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,20-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. 5-O-Caffeoylquinic acid was found to be the major phenolic compound in quince leaf extract. Quince leaf exhibited a significantly higher reducing power than green tea...
Tipo: Article Palavras-chave: Antioxidant activity; Cydonia oblonga; Quince leaf; Hemolysis; Phenolic compounds; Camellia sinensis.
Ano: 2009 URL: http://hdl.handle.net/10198/3044
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Hongos endofíticos de Camellia sinensis muestran una actividad antimicrobiana contra el tizón del arroz Magnaporthe grisea Phyton
Zhu,XJ; Hu,YF; Chen,X; Wang,YH; Fang,WP; Li,XH.
El propósito de este estudio fue evaluar la actividad antagonista de dos cepas de hongos endofíticos, Pseudocercospora kaki y Penicillium sclerotiorum, aislados de las hojas de Camellia sinensis, en relación al patógeno del tizón del arroz Magnaporthe grisea. La actividad inhibitoria de las dos cepas endófitas sobre M. grisea en cultivo dual se comparó con aquella en monocultivo. Se confirmó que el caldo y su extracto de etil acetato del cultivo dual tuvieron una actividad mucho más inhibitoria sobre M. grisea que los monocultivos de P. kaki y P. sclerotiorum. Los índices antagonistas del caldo y extracto de etil acetato del cultivo dual sobre el crecimiento del micelio de M. grisea fueron 78,02% ± 2,19% y 62,81% ± 2,29%, respectivamente, en diferentes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camellia sinensis; Hongos endofíticos; Cultivo dual; Actividad antimicrobiana; GC-MS.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000100008
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Performance, body composition, and physiological response of tambaqui fed diet with green tea extract PAB
Corrêa,Carline Barroso; Veras,Galileu Crovatto; Silva,Giselle Paula Silva da; Brabo,Marcos Ferreira; Silva,Evaldo Martins.
Abstract: The objective of this work was to evaluate the performance, body composition, and physiological state of tambaqui (Colossoma macropomum) fingerlings fed diets containing different levels of green tea (Camellia sinensis) extract (GTE). A completely randomized design was used with five treatments, different levels of GTE (0.00, 0.05, 0.30, 0.55, and 0.80 g kg-1) and three replicates. The diets were offered to fingerlings twice a day, for 90 days. The increase of dietary GTE levels promoted a positive quadratic effect on body lipids, hepatic lipids, retention of body lipids, glucose, and total triglycerides, and the minimum values of these variables were estimated with 0.61, 0.54, 0.73, 0.60, and 0.30g kg-1 GTE in the diet, respectively. There was a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camellia sinensis; Colossoma macropomum; Flavonoids; Lipid reduction; Phytotherapy; Plant extracts.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500540
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Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran Electron. J. Biotechnol.
Azadi Gonbad,Reza; Afzan,Adlin; Karimi,Ehsan; Sinniah,Uma Rani; Kumara Swamy,Mallappa.
Background Tea (Camellia sinensis), a well-known beverage is consumed frequently worldwide due to its high antioxidant properties. The present study determines the amount of phytochemicals and antioxidant activities among 12 high yielding tea clones cultivated in Iran. Results Among the 12 clones studied, tea clone Iran 100 had the highest total phenolic content and total flavonoid content with values of 8.44 ± 1.03 mg gallic acid equivalents per gram dry weight and 4.50 ± 0.16 mg rutin equivalents per gram dry weight respectively. High performance Liquid Chromatography (HPLC) analysis of phenolics and flavonoids in 12 clones revealed the presence of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epigallocatechin-gallate, (-)-epicatechingallate,...
Tipo: Journal article Palavras-chave: Camellia sinensis; DPPH assay; Methanolic extract; Total flavonoid content; Total phenolic content.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600008
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Propagação de Camellia sinensis: efeito do genótipo, estaca, substrato, recipiente e ácido indolbutírico Horticultura Brasileira
Lima,Juliana D; Bolfarini,Ana Carolina B; Silva,Silvia Helena M-G da; Moraes,Wilson da S.
Tem sido relatado que as estacas de Camellia sinensis possuem baixa capacidade de emitir raízes, motivando assim a realização de estudos básicos para otimização do processo de propagação por estacas. Assim sendo, o presente trabalho objetivou quantificar o potencial rizogênico de diferentes genótipos e o efeito da posição da estaca no ramo e incisão na base, do substrato, tamanho do recipiente e ácido indolbutírico no enraizamento de estacas semi-lenhosas dessa espécie. Para tal, foram coletados ramos dos genótipos IAC 259, F15 e Comum, em Pariquera-Açu-SP, no inverno de 2010. Em seguida, preparadas as estacas, contendo uma gema e uma folha, foram mantidas em viveiro com 70% de sombreamento. Estacas da posição basal e mediana dos ramos são as mais...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camellia sinensis; Chá; Estaquia; Enraizamento.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100012
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Resíduos da agroindústria de chá preto como substrato para produção de mudas de hortaliças Ciência Rural
Lima,Juliana Domingues; Moraes,Wilson da Silva; Mendonça,José Carlos de; Nomura,Edson Shigueaki.
O objetivo do trabalho foi determinar o efeito do resíduo do chá preto como substrato na produção de mudas de alface, tomate e pepino. Para tal, foram conduzidos testes de germinação e ensaios de crescimento empregando, respectivamente, extrato aquoso e diferentes tipos de resíduo. Nos testes de germinação, sementes de alface, tomate e pepino foram colocadas em bandejas sobre papel de filtro umedecido com água ou extrato aquoso na concentração de 1, 5 e 10%. Nos ensaios de crescimento, sementes das mesmas espécies foram colocadas em bandejas contendo: 1. vermiculita; 2. resíduo decomposto mais vermiculita; 3. resíduo decomposto e 4. resíduo bruto lavado mais vermiculita. O resíduo decomposto foi obtido após processo de decomposição do resíduo bruto e o...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Camellia sinensis; Germinação; Emergência; Crescimento; Horticultura.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600016
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White tea (Camellia sinensis) extract reduces oxidative stress and triacylglycerols in obese mice Ciênc. Tecnol. Aliment.
Teixeira,Lílian Gonçalves; Lages,Priscilla Ceci; Jascolka,Tatianna Lemos; Aguilar,Edenil Costa; Soares,Fabíola Lacerda Pires; Pereira,Solange Silveira; Beltrão,Nathalia Ribeiro Mota; Matoso,Rafael de Oliveira; Nascimento,André Márcio do; Castilho,Rachel Oliveira de; Leite,Jacqueline Isaura Alvarez.
White tea is an unfermented tea made from young shoots of Camellia sinensis protected from sunlight to avoid polyphenol degradation. Although its levels of catechins are higher than those of green tea (derived from the same plant), there are no studies addressing the relationship between this tea and obesity associated with oxidative stress.The objective of this study was to evaluate the effect of white tea on obesity and its complications using a diet induced obesity model. Forty male C57BL/6 mice were fed a high-fat diet to induce obesity (Obese group) or the same diet supplemented with 0.5% white tea extract (Obese + WTE) for 8 weeks. Adipose tissue, serum lipid profile, and oxidative stress were studied. White tea supplementation was not able to reduce...
Tipo: Info:eu-repo/semantics/article Palavras-chave: High-fat diet; Obesity; Oxidative stress; White tea; Camellia sinensis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400015
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