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Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains Electron. J. Biotechnol.
Mendoza,Héctor; de la Jara,Adelina; Freijanes,Karen; Carmona,Laura; Ramos,Ana Alexandra; de Sousa Duarte,Vanessa; Serafim Varela,João Carlos.
In the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye...
Tipo: Journal article Palavras-chave: Carotene; Carotenogenesis; Dunaliella salina; Flow cytometry; Nile red.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400005
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Evaluación del tejido adiposo de toretes en pastoreo finalizados con ensilado de caña de azúcar (Saccharum officinarum L.) Colegio de Postgraduados
Caro Hernández, José Manuel.
Buscando alternativas de alimentación que permitan reducir la pigmentación amarilla de la grasa en la canal del ganado bovino, se realizó un experimento con toretes finalizados en pastoreo, en el cual se evaluó el efecto de suplementación con ensilado de caña de azúcar (EC) en la digestibilidad, comportamiento productivo y color de la grasa en la canal. Se utilizaron 40 toretes con encastes de Brahaman, Suizo y Beefmaster (376 + 7.79 kg PV) mantenidos en pastoreo rotacional, en praderas establecidas con pasto estrella de África (Cynododn plestostachyus) por 90 d. Los tratamientos (T) fueron T1= Sólo pastoreo, T2= T1 + EC, T3= T1 + EC + 15 g de Fibrozyme®, T4= T1 + EC + 30 g de Fibrozyme®. La suplementación se realizo en corraletas individuales...
Tipo: Tesis Palavras-chave: Caña de azúcar; Ensilado; Carotenos; Colorimetría; Grasa amarilla; Toretes; Pastoreo; Enzimas fibroliticas; Maestría; Producción Agroalimentaria en el Trópico; Sugar cane; Silage; Carotene; Colorimetry; Yellow fat; Steers; Grazing; Fibrolitics enzyme.
Ano: 2007 URL: http://hdl.handle.net/10521/1433
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EXTRACTION OF OIL FROM PRESSED PALM OIL (Elaes guineensis) FIBERS USING SUPERCRITICAL CO2 Ciênc. Tecnol. Aliment.
FRANÇA,Luiz F.; MEIRELES,M. Angela A..
Residual fibers from palm oil production are a good source of carotene, since they contain more than 5% of the original oil, with about 5000 ppm of carotenoids. As carotenoids are thermosensitive molecules, supercritical CO2 can be used for oil recovery, because this technique employs low temperatures. In this work results of oil extraction experiments from pressed palm oil fibers are shown. Fibers were from AGROPALMA, an industry which is located in Tailândia (Pará, Brazil). Extractions were carried out at 200, 250 and 300 bar and at temperatures of 45 and 55oC. Oil was analyzed by UV/vis spectrophotometry for total carotene determination. Results showed a large increase in extraction rate from 200 to 250 bar and a small variation from 250 to 300 bar. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Supercritical extraction; Vegetable oil; Palm oil; Extraction kinetics; Carotene.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20611997000400008
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Optimization of callus and cell suspension cultures of Barringtonia racemosa (Lecythidaceae family) for lycopene production Scientia Agricola
Behbahani,Mandana; Shanehsazzadeh,Mehrnaz; Hessami,Mohamad Javad.
Lycopene is present in a range of fresh fruits and vegetables, especially in the leaves of Barringtonia racemosa. The traditional lycopene extraction from the plant is being employed instead of an easy propagation technique like cell culture process from the leaf explants. We intend to assess how lycopene could be extracted via tissue culture under light (illuminance: 8,200 lux under white fluorescent lamps, photoperiod 16 h per day at 25ºC) and dark. Leaf explants of Barringtonia racemosa were cultured on modified Murashige and Skoog (MS), Woody Plant Medium (WPM) and B5 media, supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D). Optimal conditions for callus induction and maintenance under both dark and light were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Powderpuff tree; Carotene; Phytochemical; Plant micropropagation; Growth hormones.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100011
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Study on the microalgal pigments extraction process: Performance of microwave assisted extraction ArchiMer
Pasquet, Virginie; Cherouvrier, Jean-rene; Farhat, Firas; Thiery, Valerie; Piot, Jean-marie; Berard, Jean-baptiste; Kaas, Raymond; Serive, Benoit; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
The performance of microwaves irradiation (MAE and VMAE) to extract pigments from two marine microalgae was compared to conventional processes (cold and hot soaking and ultrasound-assisted extraction). Pigments were quantified by RP-HPLC and extraction performance was assessed regarding rapidity, reproducibility and extraction yields. Scanning electron microscopy was used at all extraction steps to assess the impact of the process on microalgal cell integrity. Freeze-drying and pigments extraction preserved microalgae cell integrity (except sonication) and evoked agglutination in superposed cells layers. All processes performed on Dunaliella tertiolecta (chlorophyte) lead to rapid pigments extraction, and equivalent pigments extraction yields, the absence...
Tipo: Text Palavras-chave: Pigments; Microalgae; Microwave; Extraction; Chlorophyll; Fucoxanthin; Carotene; MAE; VMAE.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00029/14059/11533.pdf
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