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Aggrecan structure in amphibian cartilage BJMBR
Covizi,D.Z.; Carvalho,H.F..
The structure of the large proteoglycan present in the bullfrog epiphyseal cartilage was studied by immunochemical and biochemical methods. The isolated monomer showed a polydisperse behavior on Sepharose CL2B, with a peak at Kav = 0.14. Chondroitin sulfate chains were identified by HPLC analysis of the products formed by chondroitinase digestion and mercuric acetate treatment. These chains have approximately 38 disaccharides, a Di45:Di68 ratio of 1.6 and GalNAc4S + GalNAc4,6S are the main non-reducing terminals. Keratan sulfate was identified by the use of two monoclonal antibodies in Western blots after chondroitinase ABC treatment. A keratan sulfate-rich region (~110 kDa) was isolated by sequential treatment with chondroitinase ABC and proteases. We...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aggrecan; Bullfrog; Cartilage; Chondroitin sulfate; Epiphyseal cartilage.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200002
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Biological Effects Produced by Intense Pulsed Light (Xe-Cl) on the Cartilage of the Tongue Chick Embryo Using Various Filters International Journal of Morphology
Avila,R. E; Samar,M. E; Juri,H; Femopase,G. A; Hidalgo,M; Ferrero,J. C; Rinaldi,C; Fonseca,I; Juri,G.
The Laser used correctly in the medical practice offers clear advantages compared with traditional therapies. The improvement and even the elimination of many significant skin lesions can be achieved with reduced risks to patients. However, it is important to keep security measures and understand the possible effects on an experimental model. The chick embryo is a good model to evaluate the direct effects of non-ionizing radiation for its easy handling and availability. The purpose of this communication is to show our histological findings in organs of the chick embryo with and without protective barrier to be subjected to radiation excimer. We used the following issuers: intense pulsed light (excimer Xe-Cl laser of 308 nm wavelength). It was irradiated...
Tipo: Journal article Palavras-chave: Biological effects; Intense pulsed light (Xe-Cl); Cartilage; Tongue; Chick embryo.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400007
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Chondroitin sulfate and glucosamine in the cartilage and subchondral bone repair of dogs - Histological findings Arq. Bras. Med. Vet. Zootec.
Eleotério,R.B.; Pontes,K.C.S.; Machado,J.P.; Reis,E.C.C.; Ferreira,P.S.; Silva,M.B.; Martins,N.J.S.; Fernandes,N.A.; Borges,A.P.B..
Chondroitin and glucosamine sulfate nutraceuticals are commonly used in the management of degenerative articular disease in veterinary routine. However, there are controversies on the contribution of these substances to articular cartilage. The purpose of this study was to evaluate the efficiency of a chondroitin and glucosamine sulfate-based veterinary nutraceutical on the repair of an induced osteochondral defect in a dog femoral condyle, by macroscopic, histological and histomorphometric analyses. The nutraceutical was orally administered the day following injury induction, every 24 hours (treated group, TG, n=24), compared with animals that did not receive the product (control group, CG, n=24). Six animals per group were anaesthetized for sample...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Degenerative articular disease; Osteoarthritis; Arthrosis; Chondroprotective; Cartilage.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200325
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Clinical and experimental approaches to knee cartilage lesion repair and mesenchymal stem cell chondrocyte differentiation Biol. Res.
Montoya,Fredy; Martínez,Fernando; García-Robles,María; Balmaceda-Aguilera,Carolina; Koch,Ximena; Rodríguez,Federico; Silva-Álvarez,Carmen; Salazar,Katterine; Ulloa,Viviana; Nualart,Francisco.
Cartilage has poor regeneration capacity due to the scarcity of endogenous stem cells, its low metabolic activity and the avascular environment. Repair strategies vary widely, including microfracture, autologous or allogenic tissue implantation, and in vitro engineered tissues of autologous origin. However, unlike the advances that have been made over more than two decades with more complex organs, including vascular, cardiac or bone tissues, similar advances in tissue engineering for cartilage repair are lacking. Although the inherent characteristics of cartilage tissue, such as the lack of vascularity and low cellular diversity, suggest that it would be one of the more simple tissues to be engineered, its functional weight-bearing role and implant...
Tipo: Journal article Palavras-chave: Cartilage; Lesion; Microfracture; Stem cells.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400015
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Comparison of the effects of triamcinolone acetonide or platelet-rich plasma on expression of extracellular matrix-related genes in equine healthy chondrocytes in vitro Ciência Rural
Palma,Heloisa Einloft; Gallio,Miguel; Silva,Gabriele Biavaschi da; Cantarelli,Camila; Bertolin,Kalyne; Wolkmer,Patrícia; Wergutz,Julien; Krause,Luciana Maria Fontanari; Krause,Alexandre; Antoniazzi,Alfredo Quites; Brass,Karin Erica; Corte,Flavio Desessards De La.
ABSTRACT: In healthy cartilage, chondrocytes maintain an expression of collagens and proteoglycans and are sensitive to growth factors and cytokines that either enhance or reduce type II collagen synthesis. In osteoarthritis, pro-inflammatory cytokines, such as IL-6, induce overexpression of metalloproteinases (MMP) and decreasing synthesis of aggrecan. Use of chondroprotectors agents, such as Platelet-Rich Plasma (PRP) and triamcinolone (TA) are alternatives to reduce the progression of joint damage. In this study, we used chondrocytes extracted from metacarpophalangeal joints of healthy horses as the experimental model. Cells were treated in vitro with PRP or TA. No differences were observed between these treatments in comparison to the control group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MMPs; IL-6; Aggrecan; Cartilage; Osteoarthritis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700601
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Distribution of small proteoglycans and glycosaminoglycans in humerus-related articular cartilage of chickens BJMBR
Rodrigues,E.D.; Pimentel,E.R.; Mourão,P.A.S.; Gomes,L..
The expression of components present in the cartilaginous extracellular matrix is related to development, gender, and genotype, as well as to the biomechanical properties of each type of cartilage. In the present study, we analyzed small proteoglycans and glycosaminoglycans present in different cartilages of the chicken wing after extraction with guanidine hydrochloride or papain. Quantitative analysis of glycosaminoglycans showed a larger amount in humeral cartilage (around 200 mg/g tissue) than in articular cartilage of the radius and ulna, with 138 and 80 mg/g tissue, respectively. Non-collagenous proteins isolated were predominantly from cartilage in the proximal regions of the humerus and radius. D4 fractions obtained by ultracentrifugation were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteoglycan; Fibromodulin; Decorin; Glycosaminoglycan; Cartilage; Chicken.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300009
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Enriching a cellulose hydrogel with a biologically active marine exopolysaccharide for cell-based cartilage engineering ArchiMer
Rederstorff, E.; Rethore, G.; Weiss, P.; Sourice, S.; Beck-cormier, S.; Mathieu, E.; Maillasson, M.; Jacques, Y.; Colliec-jouault, Sylvia; Fellah, B. H.; Guicheux, J.; Vinatier, C..
The development of biologically and mechanically competent hydrogels is a prerequisite in cartilage engineering. We recently demonstrated that a marine exopolysaccharide, GY785, stimulates the in vitro chondrogenesis of adipose stromal cells. In the present study, we thus hypothesized that enriching our silated hydroxypropyl methylcellulose hydrogel (Si-HPMC) with GY785 might offer new prospects in the development of scaffolds for cartilage regeneration. The interaction properties of GY785 with growth factors was tested by surface plasmon resonance (SPR). The biocompatibility of Si-HPMC/GY785 towards rabbit articular chondrocytes (RACs) and its ability to maintain and recover a chondrocytic phenotype were then evaluated in vitro by MTS assay, cell counting...
Tipo: Text Palavras-chave: Cartilage; Hydrogel; Glycosaminoglycan; Marine polysaccharides; Chondrocytes; Tissue engineering.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00303/41449/40650.pdf
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Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage International Journal of Morphology
Hatakeyama,Yuji; Hatakeyama,Junko; Oka,Kyoko; Tsuruga,Eichi; Inai,Tetsuichiro; Anan,Hisashi; Sawa,Yoshihiko.
Amelogenin is one of the enamel matrix proteins secreted by ameloblasts during enamel formation in tooth development. Recent studies showed that the amelogenin is expressed in chondrocyte. Lysosome-associated membrane proteins (LAMPs) have been identified as binding partner proteins to amelogenin and it has been suggested they act as signaling receptors of amelogenin. The purpose of this study is to clarify the localization of amelogenin and LAMPs in growth plate cartilage and cartilaginous nodules in micromass culture. Mouse knee joints including tibia growth plate at 4 weeks old and micromass cultures of limb bud mesenchymal cells after 2 weeks were fixed in paraformaldehyde, routinely processed, sections were cut and immunostained with amelogenin,...
Tipo: Journal article Palavras-chave: Amelogenin; Lysosome-associated membrane proteins (LAMPs); Chondrogenesis; Cartilage.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200040
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Linking dietary energy and skeletal development in the horse R. Bras. Zootec.
Staniar,William Burton.
Athletic production is what is sought from the horse. As mammary development is important to the dairy cow, skeletal development is important to horses meeting their production goals. As any integrative physiologist will appreciate, the variables that come together to result in optimal skeletal development are complex. Nutrition is one of these, and it contains two broad variables; the supply of dietary nutrients and energy. This presentation will focus on dietary energy and its links with skeletal development. I propose that it is not simply the amount of dietary energy, but the way and from that that energy is supplied that impacts skeletal development. Through an understanding of how dietary energy impact skeletal development, more precise feeding...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cartilage; Equine; Glucose; Growth; Insulin; Somatotropic axis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300016
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Nestin-positive Stem Cells Participate in Chondrocyte Renewal in Healthy Adult Lung Cartilage International Journal of Morphology
Ortega-Martínez,Marta; de-la-Garza-González,Carlos; Ancer-Rodríguez,Jesús; Jaramillo-Rangel,Gilberto.
It is considered that healthy adult cartilage has little or no capacity for renewal, and that chondrocytes maintain a stable resting phenotype and resist proliferation and differentiation throughout life. Recently we found that cell turnover in lung cartilage is possible and that nestin-positive cells may have a role in it. In this paper, we report additional findings about chondrocyte renewal in lung cartilage. Lung specimens from CD1 mice at the age of 2, 6, 12, 18 or 24 months were fixed in 10% neutral-buffered formalin and paraffin-embedded. Nestin expression was examined by an immunohistochemical peroxidase-based method. We found nestin-positive cells inside of cartilage islets and cells in division very close from them. Our findings indicate that...
Tipo: Journal article Palavras-chave: Cartilage; Chondrocyte renewal; Nestin; Stem cells.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100026
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Pharmacological Modulation of Human Mesenchymal Stem Cell Chondrogenesis by a Chemically Oversulfated Polysaccharide of Marine Origin: Potential Application to Cartilage Regenerative Medicine ArchiMer
Merceron, Christophe; Portron, Sophie; Vignes-colombeix, Caroline; Rederstorff, Emilie; Masson, Martial; Lesoeur, Julie; Sourice, Sophie; Sinquin, Corinne; Colliec-jouault, Sylvia; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome.
Mesenchymal stem cells (MSCs) are considered as an attractive source of cells for cartilage engineering due to their availability and capacity for expansion and multipotency. Differentiation of MSC into chondrocytes is crucial to successful cartilage regeneration and can be induced by various biological agents, including polysaccharides that participate in many biological processes through interactions with growth factors. Here, we hypothesize that growth factor-induced differentiation of MSC can be increased by chemically oversulfated marine polysaccharides. To test our hypothesis, human adipose tissue-derived MSCs (hATSCs) were cultured in pellets with transforming growth factor (TGF)-beta 1-supplemented chondrogenic medium containing either the...
Tipo: Text Palavras-chave: Cartilage; Adipose-derived mesenchymal stem cells; Polysaccharides; Transforming growth factor-beta.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00071/18227/17457.pdf
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Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents International Journal of Morphology
Cortés-Delgado,Natalia; Pérez-Torres,Jairo; Hoyos,Julio Mario.
Transparency technique to demonstrate cartilage and skeleton has been used from years 70. This consists basically of the muscle digestion through an enzyme (trypsine), and the staining of cartilage and skeleton using alcian blue and alizarin red on specimens previously fixed to formalin. This technique can be used to study the state of the cartilage and the bones in different stages of the development and to observe abnormalitys. Also it can be used to demonstrate museum specimen skeleton preserved in liquid. In this article is showed the application of this technique for the first time in rodents and adult bats, besides some annotations to optimize the procedure.
Tipo: Journal article Palavras-chave: Chiroptera; Rodentia; Cartilage; Bone; Morphology.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400032
Registros recuperados: 12
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