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Activation of phenylalanine ammonia lyase as a key component of the antioxidative system of salt-challenged maize leaves Braz. J. Plant Physiol.
Gholizadeh,Ashraf; Kohnehrouz,B. Baghban.
Differential antioxidative activities were assessed in the leaves of two maize inbreds (A-180 and A-619) under salt stress and the subsequent recovery period. Total antioxidation test revealed that in both inbreds, this ability was sharply increased during stress period, but was slowly reverted back to the normal level during recovery. The enzymatic antioxidative analysis showed differential patterns in the activities of catalase, peroxidase and polyphenol oxidase in both maize inbreeds. Comparative analysis of the activity of phenylalanine ammonia lyase (PAL), a key enzyme at the gateway of propanoid biosynthetic pathway, suggested that propanoid compounds might be antioxidants of pivotal importance to the salt-challenged maize antioxidation system. As...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidation; Maize; Salt stress; PAL; Catalase; Peroxidase; Polyphenol oxidase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000400001
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Activity of antioxidative enzymes in watercress and Chinese cabbage plants grown under hydroponic system with brackish water Horticultura Brasileira
Lira,Raquele M; Silva,Ênio FF; Willadino,Lilia; Oliveira Filho,Ronaldo A; Andrade,Gilvany R.
ABSTRACT Underground water reserves in Semiarid Region present high salinity levels; however, scarcity of resource compels farmers to use this water for conventional agriculture. Biomass production and activity of the antioxidative defense system of Chinese cabbage and watercress were evaluated in plants grown under hydroponic NFT system with brackish water. The experimental design was completely randomized, with four replications and five water salinity levels (1.95; 3.37; 4.48; 6.82 and 7.51 dS m-1), obtained by an increasing addition of NaCl into the local water supply and nutrient salts for preparation of nutrient solution. Both watercress and Chinese cabbage plants decreased in fresh and dry shoot biomass, in relation to increase of salinity in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Naturtium officinale; Brassica pekinensis; Superoxide dismutase; Ascorbate peroxidase; Catalase.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000200205
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Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture Scientia Agricola
Pompeu,Georgia Bertoni; Gratão,Priscila Lupino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.
Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Catalase; Nickel; Superoxide dismutase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015
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Anti-oxidative and proteolytic activities and protein profile of laticifer cells of Cryptostegia grandiflora, Plumeria rubra and Euphorbia tirucalli Braz. J. Plant Physiol.
Freitas,Cleverson D. T. de; Souza,Diego P. de; Araújo,Eliane S.; Cavalheiro,Mariana G.; Oliveira,Luciana S.; Ramos,Márcio V..
In this study, proteins extracted from laticifer cells of three plants were examined by electrophoresis, mass spectrometry (MALDI-TOF) and characterized in respect of proteolytic, chitinolytic and anti-oxidative activities by means of zymography and colorimetric assays. Acidic proteins with molecular masses between 12.5 and 74.5 kDa predominated in laticifers of P. rubra. This profile was not found in laticifers of C. grandiflora and E. tirucalli. The later was poor in respect of proteins. Strong anti-oxidative activity of superoxide dismutase (E.C. 1.15.1.1) was detected in P. rubra and C. grandiflora latices, and to a lesser extent ascorbate peroxidase (E.C. 1.11.1.1) and isoforms of peroxidase were seen. Catalase (E.C. 1.11.1.6) was detected only in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Chitinase; Cysteine proteinase; Latex; Peroxidase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100002
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Atividade do sistema antioxidante e desenvolvimento de aerênquima em raízes de milho 'Saracura' PAB
Pereira,Fabricio José; Magalhães,Paulo César; Souza,Thiago Corrêa de; Castro,Evaristo Mauro de; Alves,José Donizeti.
Este trabalho teve como objetivo avaliar a influência de sucessivos ciclos de seleção do milho 'Saracura' na atividade das enzimas do sistema antioxidante, e a relação dessas enzimas com a capacidade dessa variedade em desenvolver aerênquima. Sementes de 18 ciclos de seleção intercalados do milho 'Saracura' e da cultivar BR 107, sensível à hipoxia, foram semeadas em vasos e em casa de vegetação. As plantas foram submetidas ao alagamento intermitente de dois em dois dias. As amostras de raízes foram coletadas após 60 dias e analisaram-se as atividades das enzimas peroxidase do guaiacol, peroxidase do ascorbato e catalase, além da capacidade das plantas de cada ciclo desenvolverem aerênquima. Ao longo dos ciclos, as plantas apresentaram modificações na...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Catalase; Peroxidases; Seleção massal; Tolerância ao alagamento.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000500003
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Bioactivity of Scytonema hofmanni (Cyanobacteria) in Lilium alexandrae in vitro propagation Electron. J. Biotechnol.
Zaccaro,María Cristina; Kato,Adriana; Zulpa,Gloria; Storni,Mónica Magdalena; Steyerthal,Noemí; Lobasso,Karina; Stella,Ana María.
Cyanobacteria produces bioactive compounds including plant growth regulators. Naphthalene acetic acid (NAA), a toxic substance, is a synthetic plant regulator used in micropropagation. The aim of this work was to evaluate morphogenetic and antioxidant effects produced by intra and extracellular substances from Scytonema hofmanni (Cyanobacteria) during the multiplication in vitro of Lilium alexandrae and to compare them to those produced by NAA. Intra and extracellular cyanobacterial products increased a) bulblets production reaching 83% and 78% of NAA effect, respectively; b) the bulblet diameter compared to NAA; and c) the bulblet survival due to the promotion of antioxidant activity measured as catalase, ascorbate peroxidase, and glutathione reductase...
Tipo: Journal article Palavras-chave: Ascorbate peroxidase; Bioactive substances; Bulb regeneration; Catalase; Cyanobacteria; Glutathione reductase; Lilium alexandrae; Scytonema hofmanni.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300007
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Bradyrhizobium japonicumカタラーゼ遺伝子のクローニング方法の確立 OAK
白川, 雪香; 井川, 香子; 佐藤, 哲也; 大和田, 琢二; SHIRAKAWA, Yukika; IGAWA, Keiko; SATO, Tetsuya; OHWADA, Takuji.
Palavras-chave: Bradyrhizobium; カタラーゼ; クローニング; In vitro パッケージング; Bradyrhizobium; Catalase; Cloning; In vitro packaging.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1288
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Can Triamcinolone acetonide, platelet-rich plasma, and pentosan polysulfate sodium induce oxidative stress in cultured equine chondrocytes? Ciência Rural
Palma,Heloisa Einloft; Gallio,Miguel; Silva,Gabriele Biavaschi da; Cantarelli,Camila; Wolkmer,Patrícia; Salazar,Rodrigo Fernando dos Santos; Wergutz,Julien; Krause,Luciana Maria Fontanari; Krause,Alexandre; Brass,Karin Erica; Corte,Flavio Desessards De La.
ABSTRACT: Progressive deterioration and loss of articular cartilage are the final degenerative events common to osteoarthritis (OA). Reactive oxygen species (ROS) play an important role in this chondrocyte catabolic activity, leading to cell death and matrix components breakdown. Intra-articular corticosteroid injections such as triamcinolone acetonide have been used to control pain and inflammation associated with OA. New treatments for OA, platelet-rich plasma and pentosan polysulphate sodium have also been used and further investigations are necessary to determine their safety in joint cells. In this in vitro study, the use of these three substances (triamcinolone acetonide, platelet-rich plasma, and pentosan polysulphate sodium) in healthy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Catalase; Glutathione peroxidase; Lipid peroxidation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700602
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Catalase activity of different Candida species after exposition to specific antiserum BJM
Miyasaka,Natália R.S.; Unterkircher,Carmelinda S.; Shimizu,Mario T..
Antisera were developed in rabbits after challenge with intracellular antigens of Candida albicans, C. tropicalis and C. parapsilosis. Microorganism catalase has been correlated with virulence, resistance to drugs and immunogenicity. The intracellular catalase is consistently present in strains of Candida and in this paper, the enzyme activity was analysed by PAGE after exposition to antisera. The catalases of C. albicans, C. parapsilosis and C. tropicalis were immunogenic and differed in their binding to specific antibodies raised in rabbits. Tests of cross-reactivity between different Candida species showed that when antiserum from C. albicans immunized rabbit was incubated with intracellular extracts of these three Candida species, the catalases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidiasis; Catalase; Candida; Antigen; Diagnosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100009
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Changes in biochemical characteristics and Na and K content of caper (Capparis spinosa L.) seedlings under water and salt stress JARTS
Sadeghi, Hossein; Rostami, Laleh.
In order to investigate the effect of water and salt stress on caper (Capparis spinosa L.) seedlings, a randomized complete block design with five replications was carried out in 2013 at Shiraz University, Iran. Water stress had three levels: 100 % (control), 75 %, and 5 % field capacity (FC), and five levels of salinity were applied: 0 (control), 4, 8, 12, and 18 dSm^(−1). The results indicated that salinity had a significantly negative effect on chlorophyll content of caper seedlings, while drought increased this content. The carotenoid content in caper seedlings under water and salinity stress was significantly increased. Proline and total protein content increased also under both salinity and water stress. Antioxidant enzyme activity; superoxide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant Ecophysiology caper; Catalase; Peroxidase; Salt stress; Superoxide dismutase.
Ano: 2017 URL: http://www.jarts.info/index.php/jarts/article/view/2017062752895
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Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress Braz. J. Plant Physiol.
Dey,Surjendu K.; Dey,Jayashree; Patra,Sanjukta; Pothal,Debasmita.
Wheat seedlings were grown in presence of CdCl2 (0-200 µM) and Pb(NO3)2 (0-2000 µM) separately. The growth of metal-treated seedlings was significantly depressed. The activities of antioxidative enzymes namely superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POX) were altered both in root and shoot tissues of 7-d-old seedlings. Under Cd stress, SOD activity in roots was undetectable even at the lowest Cd concentration (50 µM) whereas in shoots it declined sharply with increasing Cd levels. The activity of CAT declined to a greater extent in roots than in shoots. Even though the POX activity increased nine times in shoots, a decreasing trend was observed in root tissues due to Cd stress. Under Pb stress, the induction in SOD activity and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Heavy metals; Peroxidase; Superoxide dismutase; Triticum.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100006
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Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats BJMBR
Pereira,B.; Costa-Rosa,L.F.B.P.; Bechara,E.J.H.; Newsholme,P.; Curi,R..
Thiobarbituric acid reactant substances (TBARs) content, and the activities of glucose-6-phosphate dehydrogenase (G6PDh), citrate synthase (CS), Cu/Zn- and Mn-superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) were measured in the lymphoid organs (thymus, spleen, and mesenteric lymph nodes (MLN)) and skeletal muscles (gastrocnemius and soleus) of adrenodemedullated (ADM) rats. The results were compared with those obtained for sham-operated rats. TBARs content was reduced by adrenodemedullation in the lymphoid organs (MLN (28%), thymus (40%) and spleen (42%)) and gastrocnemius muscle (67%). G6PDh activity was enhanced in the MLN (69%) and reduced in the spleen (28%) and soleus muscle (75%). CS activity was reduced in all tissues (MLN...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epinephrine; Lymphoid organs; Superoxide dismutase; Catalase; Glutathione peroxidase; TBARs.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600016
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Changes induced by Cu2+ and Cr6+ metal stress in polyamines, auxins, abscisic acid titers and antioxidative enzymes activities of radish seedlings Braz. J. Plant Physiol.
Choudhary,Sikander P.; Bhardwaj,Renu; Gupta,Bishan D.; Dutt,Prabhu; Gupta,Rajinder K.; Kanwar,Mukesh; Dutt,Pitamber.
The present study determined the effects of copper and chromium metals on the endogenous titers of polyamines, auxins, abscisic acid and antioxidative enzyme activities in Raphanus sativus L. cv. Pusa chetki seedlings. Among polyamines, putrescine and spermidine contents were enhanced by Cu2+ metal to 62.44 and 402.8 µg g-1 f.w. respectively over control. Spermine which was not observed in control was recorded in highest concentration (1287.9 µg g-1 f.w.) in Cu2+ metal stressed seedlings. On the other hand Cr6+ metal treated seedlings showed reduced contents of putrescine (1.43 µg g-1 f.w.), cadaverine (0.09 µg g-1 f.w.), spermidine (277.99 µg g-1 f.w.) and spermine (2.29 µg g-1 f.w.) when compared to control and Cu2+ metal treated seedlings. Significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Chromium; Copper; Guaiacol peroxidase; Polyamines (PAs); Superoxide dismutase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000400006
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Effect of herbicides in the oxidative stress in crop winter species Anais da ABC (AABC)
ALVES,CARLA; COSTA,EDUARDA; SOFIATTI,JESSICA R.; FORTE,CESAR T.; WINTER,FÁBIO L.; HOLZ,CINTHIA M.; KAIZER,ROSILENE R.; GALON,LEANDRO.
ABSTRACT Most herbicides applied in crop field, stay in the soil for a period, affecting next crop or even the plants using as green manure. Nowadays, the use of herbicides grow to increase productivity, mainly in the grain producing region north of Rio Grande do Sul state. The objective of this study was to evaluate the effects of herbicides fomesafen and sulfentrazone on antioxidant system in Avena sativa1, Vicia sativa2, Raphanus sativus and Lupinus albus. The plants were exposed to varying concentrations of fomesafen3 (0, 0.125, 0.25 and 0.5 kg ha -1) and sulfentrazone (0, 0.3, 0.6 and 1.2 kg ha-1). For this, the activities of, ascorbat peroxidase, catalase and guaiacol enzymes were analyzed, and the levels of MDA were quantificated. Fomesafen and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Enzymes; Agricultural plants; PROTOX system.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401533
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Effect of Methyl Jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves Braz. J. Plant Physiol.
Soares,Alexandra Martins dos Santos; Souza,Thiago Freitas de; Jacinto,Tânia; Machado,Olga Lima Tavares.
Jasmonates are a class of plant hormones that mediate various aspects in gene and metabolic regulation, defense, stress responses, reproduction and, possibly, communication. Oxidative stress stimulates synthesis of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues. The aim of this study was to verify the effects of methyl jasmonate (JAME) treatment on the reactive oxygen species (ROS) and on the activities of H2O2 scavenging enzymes, such as superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX EC; 1.11.1.1), and guaiacol peroxidase (GPX; EC 1.11.1.7) in Ricinus communis leaves. The activity of CAT and GPX was transient while SOD activity decreased and APX increased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Methyl jasmonate; Oxidative stress; Antioxidant enzymatic defenses; Catalase; Superoxide dismutase; Peroxidase; Ricinus comunnis; Signal transduction.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000300001
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Effect of potassium sources on the antioxidant activity of eggplant¹ Rev. Bras. Ciênc. Solo
Marques,Douglas José; Broetto,Fernando; Ferreira,Mozart Martins; Lobato,Allan Klynger da Silva; Ávila,Fabricio William de; Pereira,Fabricio José.
Potassium participates in the essential processes in plant physiology, however, the effects of K sources on plant metabolism have been little studied. Also, in certain cases, K sources and concentrations may cause undesirable effects, e.g., soil salinization. The objective was to evaluate the effect of K sources and levels on the enzyme activity of the antioxidant system and protein content in eggplant (Solanum melongena L.) leaves and to determine the most suitable K sources for these physiological characteristics. The experiment was conducted in randomized blocks, in a 2 × 4 factorial design, consisting of two K sources (KCl and K2SO4) and rates (250, 500, 750, and 1000 kg ha-1 K2O), with four replications. The following variables were evaluated: plant...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Solanum melongena L.; Potassium; Superoxide dismutase; Catalase; Protein.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832014000600018
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Effect of silymarin on sodium fluoride-induced toxicity and oxidative stress in rat cardiac tissues Anais da ABC (AABC)
Nabavi,Seyed M.; Nabavi,Seyed F.; Moghaddam,Akbar H.; Setzer,William N.; Mirzaei,Morteza.
This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Glutathione; Sodium fluoride; Superoxide dismutase; Silymarin; TBARS.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400026
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Effect of Thymol on Soybean Seeds Germination: Physiological and Biochemical Analysis BABT
Lima,Eduarda Lee Ferreira; Macedo,Willian Rodrigues; Silva,Geraldo Humberto.
Abstract Higher levels of reactive species of oxygen are harmful to plant tissues. This study evaluated the action of different doses of thymol on soybean seed germination, biometric analysis and enzymatic parameters; both involved in germination process. High doses of thymol affected the plantlet growth, but not hampered the germination.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascorbate peroxidase; Catalase; Superoxide dismutase; Glycine max L; Seed vigor.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100211
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Effect of unsaturated fatty acids on myocardial performance, metabolism and morphology BJMBR
Pinotti,M.F.; Silva,M.D.P.; Sugizaki,M.M.; Diniz,Y.S.; Sant'Ana,L.S.; Aragon,F.F.; Padovani,C.R.; Novelli,E.L.B.; Cicogna,A.C..
Diets rich in saturated fatty acids are one of the most important causes of atherosclerosis in men, and have been replaced with diets rich in unsaturated fatty acids (UFA) for the prevention of this disorder. However, the effect of UFA on myocardial performance, metabolism and morphology has not been completely characterized. The objective of the present investigation was to evaluate the effects of a UFA-rich diet on cardiac muscle function, oxidative stress, and morphology. Sixty-day-old male Wistar rats were fed a control (N = 8) or a UFA-rich diet (N = 8) for 60 days. Myocardial performance was studied in isolated papillary muscle by isometric and isotonic contractions under basal conditions after calcium chloride (5.2 mM) and ß-adrenergic stimulation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fatty acids; Cardiac function; Oxidative stress; Morphology; Catalase; Superoxide dismutase.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200019
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Effects of in vitro triacontanol on growth, antioxidant enzymes, and photosynthetic characteristics in Arachis hypogaea L. Braz. J. Plant Physiol.
Verma,Aman; Malik,Chander Parkash; Gupta,Vijay Kumar; Bajaj,Bhavneet Kaur.
In vitro effects of triacontanol (TRIA) on antioxidant enzymes and photosynthetic characteristics were studied in Arachis hypogaea L. cultivars (M-13 and PBS24030). The in vitro impact of TRIA on multiplication potential was also evaluated, which was found to be best at 2.0 ml L-1 TRIA in combination with 6- Benzyl adenine (BA, 3 mg L-1) in both the groundnut cultivars. Rhizogenesis was observed in almost all the TRIA treated cultures in both the cultivars. Moreover, shoots failed to set roots, in the presence of BA. Total chlorophyll content, total soluble sugars, total soluble proteins and ascorbate peroxidase (APX; EC 1.11.1.11) activity were found to be increasing with increasing concentrations of TRIA, whether alone or in combination with BA, however,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Groundnut; Peroxidase; Vipul.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000400004
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