Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 21
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
A simple and effective pressure culture system modified from a transwell cell culture system Biol. Res.
Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian.
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is...
Tipo: Journal article Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007
Imagem não selecionada

Imprime registro no formato completo
Adenosine as a signaling molecule in the retina: biochemical and developmental aspects Anais da ABC (AABC)
PAES-DE-CARVALHO,ROBERTO.
The nucleoside adenosine plays an important role as a neurotransmitter or neuromodulator in the central nervous system, including the retina. In the present paper we review compelling evidence showing that adenosine is a signaling molecule in the developing retina. In the chick retina, adenosine transporters are present since early stages of development before the appearance of adenosine A1 receptors modulating dopamine-dependent adenylate cyclase activity or A2 receptors that directly activate the enzyme. Experiments using retinal cell cultures revealed that adenosine is taken up by specific cell populations that when stimulated by depolarization or neurotransmitters such as dopamine or glutamate, release the nucleoside through calcium-dependent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nucleoside; Receptors and transporters; Development; Cell culture; Uptake and release.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000300007
Imagem não selecionada

Imprime registro no formato completo
Amniotic cell culture during different ages of gestation for karyotype analysis in bovine Braz. J. Vet. Res. Anim. Sci.
EIRAS,Paola Ribeiro Seabra; BARRETO FILHO,João Bosco; GOLGHER,Romain Rolland; SANTOS,Sandra Regina Quintino dos.
Bovine karyotyping has become an important diagnostic tool in animal breeding. In the prenatal period it can diagnose several chromosomal abnormalities such as Robertsonian translocations, testicle feminization syndrome, gonadal dysgenesis and Klinefelter’s syndrome. An important cell source for karyotype analysis is the amniotic fluid. It has been extensively used in humans but in bovine, however, this is not the case despite its diagnostic value. Since a small percentage of cells is viable, cells and their growth conditions as well as the handling of the material should be optimal to insure a successful analysis. For this, we have compared the growth efficiency for bovine amniocytes in two media, employing cells from 10 to 14 weeks of gestation....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotypes; Cell culture; Amnion; Cattle.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400005
Imagem não selecionada

Imprime registro no formato completo
Análise morfológica de vírus produzidos pela passagem serial do baculovírus de Spodoptera frugiperda em cultivos celulares. Repositório Alice
ALMEIDA, A. F.; PEDRINI, M. R. S.; GOMES, A. C. M. M.; CASTRO, M. E. B.; RIBEIRO, B. M.; SOUZA, M. L..
2007
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Vírus; Virus; Análise morfológica; Analysis morphologic; Baculovírus; Baculovirus; Spodoptera frugiperda; Lagarta; Insecta; Cultivo celular; Cell culture.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/188687
Imagem não selecionada

Imprime registro no formato completo
Avaliação preliminar de clones de copa de seringueira. Repositório Alice
MORAES, V. H. de F..
Vinte clones de copa foram testados sobre o clone de painel CNS AM 7905. Esses clones foram obtidos de Hevea pauciflora e de híbridos dessa espécie com H. rigidifolia ou H. guianensis var. marginata, visando à seleção de copas menos volumosas, com maior aptidão ao pegamento na enxertia e menor efeito depressivo sobre a produção de borracha.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Hevea pauciflora; Seringueira; Espécie; Clone; Enxerto; Hibridação; Vigor híbrido; Mal das folhas; Doença de planta; Fungo; Brasil; Bahia; Ilhéus; Species; Clones; Grafting; Hybridization; Cell culture; Leaves; Plant diseases; Fungi.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/668081
Imagem não selecionada

Imprime registro no formato completo
Bivale mollusc cell culture ArchiMer
Mialhe, Eric; Boulo, Viviane; Grizel, Henri.
The historical background of bivalve cell culture and its potential uses in molluscan pathology are reviewed. Then, primary cultures for the study of some host-pathogen interactions at the cellular level are considered. Next, alternative methods to classical cell cultures are examined that may lead to the production of bivalve celllines.
Tipo: Text Palavras-chave: Cell culture; Pathology; Molluscs.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3122.pdf
Imagem não selecionada

Imprime registro no formato completo
Development of mussel-specific expression vectors suitable for transgenic organisms: implication on the establishment of mollusc continuous cell lines ArchiMer
Delsert, Claude; Cancela Da Fonseca, Leonor.
The main objective of this project was to develop the tools for the in-depth understanding of mollusc biology and pathology. Such a goal has been reached in other biological systems through the development of molecular biology and cell culture. Indeed, development of a mollusc cell culture system is an essential step to approach many of the problems related to mollusc pathology, in particular to use molecular biology tools to study viral infection of these organisms. No continous cell line is available from bivalves and while cell cultures have been obtained from these organisms they are difficult to develop and labor intensive, survival cultures being maintained only for a few weeks. Our goal has been to study in detail many of the parameters involved in...
Tipo: Text Palavras-chave: Pathology; Mussel-specific expression vectors; Cell culture; Mytilus galloprovincialis; Gene; Genetic marker.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00044/15540/12917.pdf
Imagem não selecionada

Imprime registro no formato completo
Diversity of enterovirus sequences detected in oysters by RT-heminested PCR ArchiMer
Dubois, Eric; Merle, Ghislaine; Roquier, Catherine; Trompette, Aurélien; Le Guyader, Soizick; Cruciere, Catherine; Chomel, Jean-jacques.
Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5' untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the...
Tipo: Text Palavras-chave: Sequence analyze; RT PCR; Cell culture; Shellfish; Enterovirus.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-763.pdf
Imagem não selecionada

Imprime registro no formato completo
Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli Biocell
Rüttler,M.E.; Yanzón,C.S.; Cuitiño,M.J.; Renna,N.F.; Pizarro,M.A.; Ortiz,A.M..
Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrhea; Escherichia coli; Cell culture; PCR (Polymerase Chain Reaction).
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200006
Imagem não selecionada

Imprime registro no formato completo
Induction of Androgenesis as a Consequence of Wide Crossing in Chickpea Open Agri
Mallikarjuna, N.,( Nalini Mallikarjuna ).
Palavras-chave: Asexual reproduction; Chromosomes; Cytokinins; Hybrids; Chickpeas; Parthenogenesis; Cell culture; Organ culture; Interspecific hybridization; Organic compounds.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3252
Imagem não selecionada

Imprime registro no formato completo
Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis ArchiMer
Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry.
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater...
Tipo: Text Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf
Imagem não selecionada

Imprime registro no formato completo
Primary culture of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae) Braz. J. Vet. Res. Anim. Sci.
SALVO,Ligia Maria; MALUCELLI,Maria Ivette Carboni; RICHARTZ,Rosária Regina Tesoni B.; BACILA,Metry.
In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used: enzyme cell dissociation by means of a system containing trypsin and versene 0.25%; by means of bovine fetal serum and by the use of blood serum obtained from adult specimens of M. roosevelti. The cell medium used has been a modified F10-199 by the addition of 2 mM L-glutamine, 10 µg/mL insulin, 50 µg/mL fibronectin; and antibiotics. The best results in terms of cell dissociation as well as in terms of adhesion and confluence of the cells monolayer has been achieved by means of the enzymatic cell dissociation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Liver cells; Cell culture; Cells; Pisces; Metynnis roosevelti.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000500004
Imagem não selecionada

Imprime registro no formato completo
Produção de antígeno de lentivírus de pequenos ruminantes através da cultura celular de membrana nictitante caprina. Repositório Alice
AZEVEDO, D. A. A. de; ARAÚJO, J. F.; SOUSA, A. L. M. de; DIAS, R. P.; SOUZA, T. S. de; PINHEIRO, A. A.; PINHEIRO, R. R..
Resumo: Os Lentivírus de Pequenos Ruminantes (LVPR) pertencem à família Retroviridae e causam enfermidades crônicas e progressivas, conhecidas como artrite-encefalite caprina em caprinos e maedi-visna em ovinos. As células da linhagem monocítico fagocitária são os principais alvos do vírus no organismo animal, que não consegue desenvolver resposta imune curativa. Baseando-se no fato de que a membrana nictitante (MN) sofre naturalmente infecção, no organismo do animal, por estes vírus, objetivou-se avaliar se essas são passiveis para o cultivo e se possuem bom crescimento em cultura, visando à produção de antígeno de lentivírus caprino (LVC) para técnica de imunodiagnóstico. Sendo assim, realizou-se explant de células de MN de um animal negativo para LVPR....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cultivo celular; IDGA; LVPR; Cultura microbiana; Lentivirose; AGID; SRLV; Cultura de célula; Caprino; Antígeno; Lentivirus; Cell culture; Antigens; Goats.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1032546
Imagem não selecionada

Imprime registro no formato completo
Protoplast culture and transformation in rice (Oryza sativa L.) International Rice Research Institute
Wu, Chuanyin.
138 leaves: ill. photographs. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Cell culture; Protoplasts; Genetic transformation; Plant breeding.
Ano: 1992 URL: http://hdl.handle.net/123456789/822
Imagem não selecionada

Imprime registro no formato completo
Protoplast culture and use of peg and biolistic methods of transformation in rice (Oryza sativa L.) International Rice Research Institute
Kim, Hyun Soon.
105 leaves : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Cell culture; Protoplasts; Genetic transformation; Biolistics; Genetic techniques and protocols.
Ano: 1993 URL: http://hdl.handle.net/123456789/740
Imagem não selecionada

Imprime registro no formato completo
Protoplast culture in indica, japonica and wild rices and production of transgenic plants in a japonica variety (Zhonghua 10) International Rice Research Institute
Zhang, Shiping.
132 leaves : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Cell culture; Protoplasts; Wild relatives; Transgenic plants; Varieties; Plant breeding; Zhonghua 10 (rice variety).
Ano: 1992 URL: http://hdl.handle.net/123456789/821
Imagem não selecionada

Imprime registro no formato completo
Protoplast culture, plant regeneration and initial studies on transformation in rice (Oryza sativa L.) International Rice Research Institute
Wang, Qinfang.
111 leaves : ill. Thesis (M.S.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Cell culture; Protoplasts; Regeneration; Genetic transformation; Plant breeding.
Ano: 1992 URL: http://hdl.handle.net/123456789/825
Imagem não selecionada

Imprime registro no formato completo
Sarcocystis : Development of Sporocysts in Cell Culture OAK
Odening, Klaus; Frölich, Kai; Kirsch, Brigitte; Bockhardt, Ingrid.
Palavras-chave: Sarcocystis; Cell culture; Gamogony; Sporogony.
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/256
Imagem não selecionada

Imprime registro no formato completo
Variability in bioavailable iron in hand-pounded traditional rice varieties from a highland village in northern Thailand International Rice Research Institute
Prom-u-thai, C.; Rerkasem, B..
p.47-48
Palavras-chave: Iron; Bioavailability; In vitro digestibility; Cell culture; Ascorbic acid; Phytates; Varieties; Thailand.
Ano: 2004 URL: http://hdl.handle.net/123456789/770
Imagem não selecionada

Imprime registro no formato completo
Viral contamination and depuration of oysters under field conditions ArchiMer
Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L.
Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine...
Tipo: Text Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf
Registros recuperados: 21
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional