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Adsorption, immobilization and activity of cellulase in soil: the impacts of maize straw and its humification BABT
Sinegani,Ali Akbar Safari; Sinegani,Mahboobe Safari.
The present work aimed to study some aspects of sorption and immobilization of cellulase molecules on soil components by the analysis of the reactions of cellulase in a soil treated with different levels of maize residue and incubated for 90 days. The analysis of variance showed that the effects of the treatments of maize straw, incubation time and their interaction on cellulase adsorption, desorption and immobilization were statistically significant. The adsorption and immobilization capacities of soil by application of maize straw increased significantly. However they decreased with decreasing the soil organic matter (SOM) after 45 days of incubation. The desorption of adsorbed cellulase molecules from the soil by washing with distilled water depended on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize straw; Humification; Cellulase; Sorption; Immobilization; Activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600001
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Alkali pretreated of wheat straw and its enzymatic hydrolysis BJM
Han,Lirong; Feng,Juntao; Zhang,Shuangxi; Ma,Zhiqing; Wang,Yonghong; Zhang,Xing.
The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Cellulose; Fermentation; Hydrolysis; Lignocellulosic; Pretreatment.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100006
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Assessment of cellulolytic microorganisms in soils of Nevados Park, Colombia BJM
Avellaneda-Torres,Lizeth Manuela; Pulido,Claudia Patricia Guevara; Rojas,Esperanza Torres.
A systematized survey was conducted to find soil-borne microbes that degrade cellulose in soils from unique ecosystems, such as the Superpáramo, Páramo, and the High Andean Forest in the Nevados National Natural Park (NNNP), Colombia. These high mountain ecosystems represent extreme environments, such as high levels of solar radiation, low atmospheric pressure, and extreme daily changes in temperature. Cellulolytic activity of the microorganisms was evaluated using qualitative tests, such as growth in selective media followed by staining with congo red and iodine, and quantitative tests to determine the activity of endoglucanase, β-glucosidase, exoglucanase, and total cellulase. Microorganisms were identified using molecular markers, such as the 16S rRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Endoglucanase; Β-glucosidase; Exoglucanase; Páramo; High Andean Forest.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400011
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Atividade de amilases e celulases em sementes de duas espécies de Sterculia L. Repositório Alice
ALVES, J. P. de A.; SALLES, H. O.; FIGUEIREDO, M. F.; JULIÃO, M. S. da S.; ANDRADE, L. B. da S..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sterculia striata; Sterculia foetida; Glicosidase; Amilase; Amido; Celulose; Enzima; Semente; Extrato vegetal; Atividade enzimática; Amylases; Cellulase; Glycosidases; Sterculia; Plant Extract; Enzyme activity; Seeds.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/975117
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Atividade de glicosidases em sementes de Caesalpinia pyramidalis Tul. Repositório Alice
OLIVEIRA, T. dos R. de; SOUSA, E. M. de; BRITO, M. S. de; FIGUEIREDO, M. F.; SALLES, H. O.; ANDRADE, L. B. da S..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Glicosidase; Enzimas hidrolíticas; Amilase; Celulase; Enzima; Catingueira; Caesalpinia pyramidalis; Amylases; Cellulase; Enzymes; Hydrolyases.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/973718
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Bioconversion and saccharification of some lignocellulosic wastes by Aspergillus oryzae ITCC-4857.01 for fermentable sugar production Electron. J. Biotechnol.
Begum,Most. Ferdousi; Alimon,Abdul Razak.
The recent interest in bioconversion of agricultural and industrial wastes to chemical feedstock has led to extensive studies on cellulolytic enzymes produced by microorganisms. In the present study three lignocellulosic substrates viz. sugarcane bagasse, sawdust and water hyacinth were pre-treated with alkali and enzyme and their effect on bioconversion has been investigated. The ability of selected substrates for induction of cellulase enzyme by A. oryzae ITCC 4857.01 and for the potentiality of the induced enzyme to saccharify the substrates were also assessed. The maximum degree of conversion of substrate (0.415%) and improved specific substrate consumption (0.99 g substrate/g dry biomass) was exhibited in sugarcane bagasse after alkali treatment at 96...
Tipo: Journal article Palavras-chave: Aspergillus oryzae; Biomass; Cellulase; Lignocellulosic substrates; Pre-treatment; Saccharification.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500003
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Bioethanol production from rice straw residues BJM
Belal,Elsayed B..
A rice straw -cellulose utilizing mold was isolated from rotted rice straw residues. The efficient rice straw degrading microorganism was identified as Trichoderma reesei. The results showed that different carbon sources in liquid culture such as rice straw, carboxymethyl cellulose, filter paper, sugar cane bagasse, cotton stalk and banana stalk induced T. reesei cellulase production whereas glucose or Potato Dextrose repressed the synthesis of cellulase. T. reesei cellulase was produced by the solid state culture on rice straw medium. The optimal pH and temperature for T. reesei cellulase production were 6 and 25 ºC, respectively. Rice straw exhibited different susceptibilities towards cellulase to their conversion to reducing sugars. The present study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Trichoderma reesei; Rice straw residues; Enzymatic hydrolysis; Bioethanol.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100033
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Bioprospecting thermophiles for cellulase production: a review BJM
Acharya,Somen; Chaudhary,Anita.
Most of the potential bioprospecting is currently related to the study of the extremophiles and their potential use in industrial processes. Recently microbial cellulases find applications in various industries and constitute a major group of industrial enzymes. Considerable amount of work has been done on microbial cellulases, especially with resurgence of interest in biomass ethanol production employing cellulases and use of cellulases in textile and paper industry. Most efficient method of lignocellulosic biomass hydrolysis is through enzymatic saccharification using cellulases. Significant information has also been gained about the physiology of thermophilic cellulases producers and process development for enzyme production and biomass...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignocelluloses; Bioethanol; Cellulase; Thermophiles.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300001
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Caracterização da atividade lignocelulolítica de enzimas ruminais de ovinos quanto à ação de agentes redutores. Repositório Alice
BARBOSA, I. C.; RIBEIRO, R. P.; SOUSA, A. M. P.; SALLES, H. O..
Enzimas termoestáveis são importantes para uso nos bioprocessos industriais. As enzimas lignocelulolíticas de micro-organismos ruminais aderidas à fibra do bagaço de cana-de-açúcar (BCA) no rúmen de ovinos Morada Nova foram inicialmente caracterizadas como termoestáveis, mantendo sua atividade mesmo após 10 minutos a 90 oC. Como a presença de pontes de dissulfeto nas enzimas pode estar envolvida no mecanismo de estabilidade dessas moléculas, o objetivo desse trabalho foi caracterizar a atividade das proteínas aderidas à fibra (PAFs) do BCA obtida do conteúdo ruminal de ovinos Morada Nova, quanto à ação dos agentes redutores de pontes dissulfeto.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Raça Morada Nova; Xilanase; Pontes dissulfeto; Biomassa lignocelulósica; Celulase; Microrganismo; Ovino; Sheep; Microorganisms; Cellulase.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084739
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Cellulase activities during decomposition of a submerged aquatic macrophyte (Utricularia breviscapa): a microcosm assay BJM
Cunha-Santino,Marcela B. da; Bianchini Jr.,Irineu.
Decomposition of lignocellulosic detritus at enzymatic level is the rate-limiting step during aquatic macrophyte decomposition. Assays were carried out to evaluate cellulase activity during decomposition of Utricularia breviscapa; the assays included the following C-sources: leachate, lignocellulosic matrix and integral detritus. The incubations comprised U. breviscapa C-sources added to Óleo lagoon (21º 36'S and 47º 49'W) water maintained in the dark under anaerobic condition at 15ºC, 20ºC, 25ºC and 30ºC. Cellulase activity were monitored during 180 days, with the activity showing a time evolution that depended on the temperature and type of detritus: (i) cellulase activity tended to occur earlier with increasing temperatures, but it was less intense than...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Utricularia breviscapa; Cellulose.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200008
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Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose BABT
Pereira Júnior,José Antônio de Sousa; Correia,Marcos José; Oliveira,Neiva Tinti de.
Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lentinula edodes; Cellulase; Liquid medium cultivation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300004
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Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation BJM
Heck,Júlio X.; Hertz,Plinho F.; Ayub,Marco A.Z..
In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max) protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-State cultivation; Cellulase; Xylanase; Soybean residue; Amazon biodiversity.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300005
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Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains Electron. J. Biotechnol.
Lin,Ling; Kan,Xianzhao; Yan,Hao; Wang,Danni.
The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from...
Tipo: Journal article Palavras-chave: Bacillus thuringiensis; Cellulase; Cellulose-degrading; Characterization.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002
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Chitinase and cellulase activity from Bacillus thuringiensis strains. Repositório Alice
DUMAS, V. F.; GOLDENBERG, C. doa S.; MARTINS, E. S.; PRACA, L. B.; QUEIROZ, P. R.; PONTES, R. G. M. S. de; MELO, F. R..
bitstream/item/183203/1/974-4593-1-PB.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cellulase; Cry proteins; Bacillus Thuringiensis; Chitinase.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/884059
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Coexpression of rumen fungal xylanase and bifunctional cellulase genes in Escherichia coli BABT
Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil.
ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407
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Doses of enzyme complex in a high-energy diet on performance and carcass traits of feedlot steers R. Bras. Zootec.
Vigne,Gabriela Letícia Delai; Neumann,Mikael; Santos,Leslei Caroline; Dochwat,André; Venancio,Bruno José; Heker Junior,Julio Cezar; Ueno,Robson Kyoshi; Souza,André Martins de.
ABSTRACT The objective of this study was to evaluate the production performance and carcass traits of feedlot steers fed a high-energy diet supplemented with doses of an enzyme complex (0, 2.5, 5.0, and, 7.5 g animal−1 day−1). Thirty-two ½ Angus ½ Nellore crossbred steers at an average age of 12 months and an average initial weight of 422 kg, were kept in a feedlot for 77 days. The roughage-free diet was composed of a mixture of whole corn grain and a protein-mineral-vitamin mix at a constant ratio of 85:15, on a dry matter basis. A completely randomized block design was adopted, consisting of four treatments and four replicates, in which each replicate was represented by a stall with two animals. Each gram of product added to the diet led to a decrease of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Digestibility; Feed intake; Non-carcass component; Ultrasonography.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100612
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Endoglucanase production with the newly isolated Myceliophtora sp. i-1d3b in a packed bed solid state fermentor BJM
Zanelato,A. I.; Shiota,V. M.; Gomes,E.; Silva,R. da; Thoméo,J. C..
This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the fungus Myceliophtora sp. I-1D3busing a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium. Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50ºC), initial moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1:1, 7:3, and 9:1). The highest enzyme activities in plastic bags were obtained using the substrate proportion of 7:3, 50ºC temperature, and 80% initial moisture content (878 U/grams of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported. For the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Xylanase; Myceliophthora sp.; Packed bed; Solid state-fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400038
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Filamentous fungi and media for cellulase production in solid state cultures BJM
Kilikian,B.V.; Afonso,L.C.; Souza,T.F.C.; Ferreira,R.G.; Pinheiro,I.R..
Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Productivity; Solid media; Sugarcane bagasse; Myceliophtora sp.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100041
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Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts BJM
Oliveira,Lilian C.G.; Ramos,Patricia Locosque; Marem,Alyne; Kondo,Marcia Y.; Rocha,Rafael C.S.; Bertolini,Thiago; Silveira,Marghuel A.V.; Cruz,João Batista da; Vasconcellos,Suzan Pantaroto de; Juliano,Luiz; Okamoto,Debora N..
Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Halophilic; Protease; Lipase; Amylase; Cellulase.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200347
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Impact of different grazing systems for bovine cattle on the soil microbiological and chemical characteristics R. Bras. Zootec.
Garcia,Martha Regina Lucizano; Sampaio,Alexandre Amstalden Moraes; Nahas,Ely.
The objective of this work was to study the effect of grazing systems: rotational, continuous and the absence of cattle, in pastures of Brachiaria brizantha on microbial activity (respiratory, potential nitrification, solubilizing, and enzymatic) and on the chemical attributes (contents of organic carbon, total nitrogen, organic phosphorus, and moisture) of Eutrustox soil over one year. Eight Canchim breed animals were used. The samples were collected in the four seasons from 2005 to 2006. The average of organic C, organic P, and moisture contents, as well as potential nitrification and solubilizing activity and urease and acid phosphatase activities were significantly higher in the spring and/or summer than in autumn and/or winter. The total nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Microbial respiratory activity; Phosphatase; Potential nitrification; Urease.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000700024
Registros recuperados: 36
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