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A thermostable chitinase from the antagonistic Chromobacterium violaceum that inhibits the development of phytopathogenic fungi. Repositório Alice
SOUSA, A. J. S.; GRANJEIRO, T. B.; MOREIRA, ANA CRISTINA DE OLIVEIRA MONTEIRO; LOBO, M. D. P.; SOUSA, B. L. DE; FREIRE, J. E. DA C.; MONTEIRO JÚNIOR, J. E.; SOUSA, J. S. DE; SILVA, C. de F. B. da.
bitstream/item/206287/1/ART19019.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Quitina; Hidrolase de glicosídeo; Proteína recombinante; Peptídeo sinal; Expressão heteróloga; Atividade antifúngica; Chitin; N-glycoside hydrolases; Recombinant proteins; Signal peptide.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116058
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Aislamiento y caracterización de bacterias quitinolíticas ruminales Colegio de Postgraduados
Piloni Martini, Javier.
Los principales objetivos del presente estudio fueron aislar y seleccionar bacterias ruminales quitinolíticas con alta capacidad para degradar quitina pura y caparazón de camarón in vitro, e identificar genéticamente a las bacterias seleccionadas usando secuencias de su gen 16S rARN amplificado mediante PCR. El aislamiento de las bacterias ruminales se inicio con un cultivo mixto liofilizado de bacterias quitinolíticas (CMBQ) obtenido de borregos alimentados con una dieta con 25% de caparazón de camarón. Se requirieron tres aislamientos progresivos para obtener dos bacterias ruminales puras. En el primer aislamiento se obtuvieron seis consorcios bacterianos: BQT1, BQT2, BQT3, BQT4, BQT6 y BQT6 , a partir de CMBQ. Por su alta capacidad para...
Palavras-chave: Rumen; Quitina; Caparazón de camarón; Bacterias quitinolíticas; 16S rARN Rumen; Chitin; Shrimp shell waste; Chitinolytic bacteria; 16S r RNA.
Ano: 2012 URL: http://hdl.handle.net/10521/810
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Aislamiento y caracterización de bacterias quitinolíticas ruminales Colegio de Postgraduados
Piloni Martini, Javier.
Los principales objetivos del presente estudio fueron aislar y seleccionar bacterias ruminales quitinolíticas con alta capacidad para degradar quitina pura y caparazón de camarón in vitro, e identificar genéticamente a las bacterias seleccionadas usando secuencias de su gen 16S rARN amplificado mediante PCR. El aislamiento de las bacterias ruminales se inicio con un cultivo mixto liofilizado de bacterias quitinolíticas (CMBQ) obtenido de borregos alimentados con una dieta con 25% de caparazón de camarón. Se requirieron tres aislamientos progresivos para obtener dos bacterias ruminales puras. En el primer aislamiento se obtuvieron seis consorcios bacterianos: BQT1, BQT2, BQT3, BQT4, BQT6 y BQT6 , a partir de CMBQ. Por su alta capacidad para degradar...
Tipo: Tesis Palavras-chave: Rumen; Quitina; Caparazón de camarón; Bacterias quitinolíticas; 16S rARN; Rumen; Quitina; Caparazón de camarón; Bacterias quitinolíticas; 16S rARN; Rumen; Chitin; Shrimp shell waste; Chitinolytic bacteria; 16S r RNA.
Ano: 2008 URL: http://hdl.handle.net/10521/1170
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Aislamiento y caracterización de bacterias quitinolíticas ruminales Colegio de Postgraduados
Piloni Martini, Javier.
Los principales objetivos del presente estudio fueron aislar y seleccionar bacterias ruminales quitinolíticas con alta capacidad para degradar quitina pura y caparazón de camarón in vitro, e identificar genéticamente a las bacterias seleccionadas usando secuencias de su gen 16S rARN amplificado mediante PCR. El aislamiento de las bacterias ruminales se inicio con un cultivo mixto liofilizado de bacterias quitinolíticas (CMBQ) obtenido de borregos alimentados con una dieta con 25% de caparazón de camarón. Se requirieron tres aislamientos progresivos para obtener dos bacterias ruminales puras. En el primer aislamiento se obtuvieron seis consorcios bacterianos: BQT1, BQT2, BQT3, BQT4, BQT6 y BQT6 , a partir de CMBQ. Por su alta capacidad para...
Palavras-chave: Rumen; Quitina; Caparazón de camarón; Bacterias quitinolíticas; 16S rARN Rumen; Chitin; Shrimp shell waste; Chitinolytic bacteria; 16S r RNA.
Ano: 2012 URL: http://hdl.handle.net/10521/787
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Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells. Repositório Alice
GALLÃO, M. I.; CORTELAZZO, Â. L.; FEVEREIRO, M. P. S.; BRITO, E. S. de.
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Chitin; Elicitation; Peroxidase; PAL; Sugarcane; Tissue cultura.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/875372
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Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells BABT
Gallão,Maria Izabel; Cortelazzo,Ângelo Luiz; Fevereiro,Manuel Pedro Salema; Brito,Edy Sousa de.
Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitin; Elicitation; Peroxidase; PAL; Sugarcane; Tissue culture.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002
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Chitin/toxicant interactions and microbial degradation within estuarine ecosystems. ArchiMer
Portier, R; Meyers, S.
The environmental effect and fate of several agricultural and industrial source toxicants were evaluated in controlled laboratory microcosm systems in the presence of the aminopolysaccharide polymer, chitin. Toxicants examined included methyl parathion, phenol, pentachlorophenol, 2,4 dichlorophenol, 1,2 dichloroethane and Kepone. Microcosm operating parameters, including pH/Eh, salinity, temperature, flow rate and sediment/water interface conditions, were based on comparable in situ measurements. Fate analysis predictions were based on mineralization of the parent toxicant compound as measured by chromatographic analysis or super(14)CO sub(2) expiration. Chitin, amended to continuous flow microcosms, promoted either cometabolic or cooxidative...
Tipo: Text Palavras-chave: Controlled conditions; Chemical pollutants; Industrial wastes; Pesticides; Metabolism; Food webs; Biodegradation; Ecosystems; Microorganisms; Environmental effects; Brackishwater pollution; Estuaries; Toxicants; Chitin.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1011.pdf
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Combined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut Open Agri
Manjula, K..
Palavras-chave: Biological control; Biochemical compounds; Bacteria; Poultry equipment; Fungi; Enzymes; Fungicides; Antibiotics; Seed treatment; Chitin.
Ano: 2004 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3322
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Design Polysaccharides of Marine Origin: Chemical Modifications to Reach Advanced Versatile Compounds ArchiMer
Chopin, Nathalie; Guillory, Xavier; Weiss, Pierre; Le Bideau, Jean; Colliec-jouault, Sylvia.
Polysaccharides are among the most abundant macromolecules on Earth. These polymers are easily obtained from various marine resources such as algae, microorganisms and crustacean shells. The structure of these natural carbohydrates is innovative and quite complex. Marine biopolymers represent key scaffolds toward large challenging fields, such as biomedical applications (glycosaminoglycans, regenerative medicine and drug delivery) and tailored biomaterials. Chemical modifications can be applied to modify their final properties in a specific purpose. New functional glycans are achievable and represent a real potential with their intrinsic biocompatibility and biodegradability. Hydroxyl groups are ubiquitous in polysaccharides structure and involved in most...
Tipo: Text Palavras-chave: Alginates; Carrageenans; Chemical modification; Chitin; Chitosan; Grafting; Marine polysaccharide; Polymer.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00199/31027/29902.pdf
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Efecto de quitina y quitosano sobre huevos y juveniles de nemátodos formadores de nódulos radiculares, Nacobbus aberrans y Meloidogyne incognita bajo condiciones de in vitro e in vivo Colegio de Postgraduados
Sánchez Portillo, Juan Fernando.
El nematodo agallador Meloidogyne incognita Kofoid y White, 1919, Chitwood, 1949, distribuido en 23 de los 32 estados del país y Nacobbus aberrans Thorne 1935, Thorne and Allen, 1944, distribuido en 10 de 32 estados de la República Mexicana, su combate para ambas especies, casi siempre se hace aplicando diferentes agroquímicos, los cuales algunos ya tienen problemas de registro. Se necesita evaluar nuevos productos que sean otra alternativa a su manejo y mas amigables al ambiente. En el presente trabajo se evaluó el efecto de quitina-quitosano con diferentes grados de desacetilación, biopolímeros naturales que poseen propiedades nematicidas y utilizados como reguladores de la población de estos fitonematodos; los estudios se hicieron in vitro, sobre la...
Palavras-chave: Biopolímero natural; Meloidogyne incognita; Nacobbus aberrans; Desacetilación; Control; Quitina; Quitosano; Chitin; Chitinase; Natural biopolymer; Chitosan; Deacetylation; Fitopatología; Maestría.
Ano: 2010 URL: http://hdl.handle.net/10521/55
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Efecto de quitina y quitosano sobre huevos y juveniles de nemátodos formadores de nódulos radiculares, Nacobbus aberrans y Meloidogyne incognita bajo condiciones de in vitro e in vivo Colegio de Postgraduados
Sánchez Portillo, Juan Fernando.
El nematodo agallador Meloidogyne incognita Kofoid y White, 1919, Chitwood, 1949, distribuido en 23 de los 32 estados del país y Nacobbus aberrans Thorne 1935, Thorne and Allen, 1944, distribuido en 10 de 32 estados de la República Mexicana, su combate para ambas especies, casi siempre se hace aplicando diferentes agroquímicos, los cuales algunos ya tienen problemas de registro. Se necesita evaluar nuevos productos que sean otra alternativa a su manejo y mas amigables al ambiente. En el presente trabajo se evaluó el efecto de quitina-quitosano con diferentes grados de desacetilación, biopolímeros naturales que poseen propiedades nematicidas y utilizados como reguladores de la población de estos fitonematodos; los estudios se hicieron in vitro, sobre la...
Palavras-chave: Biopolímero natural; Meloidogyne incognita; Nacobbus aberrans; Desacetilación; Control; Quitina; Quitosano; Chitin; Chitinase; Natural biopolymer; Chitosan; Deacetylation; Fitopatología; Maestría.
Ano: 2010 URL: http://hdl.handle.net/10521/55
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Efeito da quitosana na anatomia radicular de híbridos transgênico e não transgênico de milho. Repositório Alice
SOUZA, T. C. de; MARTINS, M.; VERONEZE JUNIOR, V.; CARVALHO, M.; MAGALHAES, P. C..
2016
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Biopolímero; Epiderme radicular; Quitina; Biopolymers; Chitin.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1054630
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Effects of Emerita brasiliensis flour supplementation on normotensive (Wistar) and spontaneously hypertensive rats (SHR) BABT
Cavargere,Victor Agati; França,Camille Feitoza; Cardoso,Ricardo; Vianna,Lucia Marques.
Several studies characterize the connection of overweight and obesity with chronic diseases. Therefore, new alternatives are being studied for controlling hypertension, such as chitin and chitosan fibers, commonly found on crustacean's carapace like Emerita brasiliensis. Rats from two different strains were divided into control and supplemented groups (n=6). The Wistar strain experiment started with a 14-days baseline period, followed by supplementation of E.brasiliensis flour added to the diet in the doses of 5, 10 and 20%, for a 14-days period each. With the optimal dose of 20%, the study was undertaken with SHR rats, starting with a 7-days baseline period, followed by three weeks of supplementation. Data were evaluated using one-way ANOVA and p<0.05...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitin; Chitosan; Emerita brasiliensis; Blood pressure.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400006
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Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium Electron. J. Biotechnol.
Stamford,Thayza Christina Montenegro; Stamford,Tânia Lucia Montenegro; Stamford,Newton Pereira; Barros Neto,Benicio de; Campos-Takaki,Galba Maria de.
Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth,...
Tipo: Journal article Palavras-chave: Biopolymers; Chitin; Chitosan.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100006
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Heavy metal biosorption by chitin and chitosan isolated from Cunninghamella elegans (IFM 46109) BJM
Franco,Luciana de Oliveira; Maia,Rita de Cássia C.; Porto,Ana Lúcia F.; Messias,Arminda Sacconi; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de.
Chitin and chitosan were extracted from mycelial biomass of Cunninghamella elegans and the performance for copper, lead and iron biosorption in aqueous solution was evaluated. The growth curve of C. elegans was accomplished by determination of biomass, pH, glucose and nitrogen consumption. Chitin and chitosan were extracted by alkali-acid treatment and the yields were 23.8 and 7.8%, respectively. For the adsorption analysis, the process of heavy uptake metal sorption was evaluated using polysaccharides solutions (1% w/v). The rate of metallic biosorption was dependent upon the concentration and pH of metal solutions, and the best results were observed with pH 4.0. Chitosan showed the highest affinity for copper and chitin for iron adsorption. The results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cunninghamella elegans; Chitin; Chitosan; Bioremediation; Biosorption.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200013
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Induction of phenolics and defense-related enzymes in coconut (Cocos nucifera L.) roots treated with biocontrol agents Braz. J. Plant Physiol.
Karthikeyan,M.; Radhika,K.; Mathiyazhagan,S.; Bhaskaran,R.; Samiyappan,R.; Velazhahan,R..
The effect of soil application of biocontrol agents (Pseudomonas fluorescens, Trichoderma viride and T. harzianum) in combination with chitin on induction of phenolics and defense enzymes in coconut roots infected with Ganoderma lucidum, the causal agent of Ganoderma disease, was investigated. Soil application of these biocontrol formulations in combination with chitin induced a significant increase in the activities of peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), chitinase and beta-1,3-glucanase in the G. lucidum infected palms. Activities of both PAL and PO reached maximum levels within 3 d while the activity of PPO reached the maximum level 6 d after application of a mixture of P. fluorescens, T. viride and chitin....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitin; Ganoderma disease; Induced systemic resistance; Pathogenesis-related proteins; Phenolics; Pseudomonas fluorescens; Trichoderma.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000300003
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Producción de enzimas extracelulares y esporas de Lecanicillumlecanil en arroz - quitina de camarón y efecto sobre Brevycorine brassicae Colegio de Postgraduados
Enriquez Vara, Jhony Navat.
El objetivo del presente estudio fue utilizar arroz mezclado con quitina cruda de camarón como sustrato e inductor de quitinasas y proteasas de Lecanicillium lecanii en fermentación en medio sólido, además, se evaluó el efecto patogénico de conidios y enzimas producidas en medio sólido sobre el pulgón cenizo de la col. Cinco aislamientos de L. lecanii fueron caracterizados considerando el tamaño del conidio, la velocidad de germinación (GT5, GT50, y GT95), crecimiento radial en placa en dos medios de cultivo, esporulación y virulencia. La correlación entre el tamaño del conidio, la tasa máxima de crecimiento en placa y la tasa específica de crecimiento fueron altamente significativas. L. lecanii ATCC 26854 fue seleccionado por presentar...
Tipo: Tesis Palavras-chave: Lecanicillium lecanii; Brevicoryne brassicae; Fermentación sólida; Exo enzimas; Quitina; Quitinasas; Proteasas; Control biológico; Virulencia; Maestría; Entomología y Acarología; Solid state fermentation; Exoenzymes; Chitin; Chitinases; Proteases; Bological control; Vrulence.
Ano: 2008 URL: http://hdl.handle.net/10521/1497
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Purification de la chitine par hydrolyse enzymatique à partir de coproduits de crevette Penaeus vannamei. Caractérisations des produits et optimisation du procédé ArchiMer
Le Roux, Karine.
The objective of this study was to optimize the extraction of chitin by acid proteolysis. The novelty of the method is based on the stabilization of pH by the balance between substrate composition and the acid solvent. This principle allows a simultaneous demineralization and deproteinization, the two main reactions associated with the purification of chitin. To evaluate the performance of this purification, the composition of the substrate and products was characterized. Different methods of quantification of chitin and proteins have been compared. As traditional assays were not satisfaying, a direct method of amino acids determination by gaz chromatography was selected to estimate the amount of protein. The estimate of chitin amount was based on indirect...
Tipo: Text Palavras-chave: Coproduits de crustacés; Hydrolyse enzymatique; Protéolyse; Chitine; Extraction; Cinétique; Déminéralisation; Déprotéinisation; Crustacean by-products; Enzymatic hydrolysis; Proteolysis; Chitin; Extraction; Kinetic; Demineralization; Deproteinization.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00087/19828/17470.pdf
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Response to chitin in suspension-cultured Citrus aurantium cells Braz. J. Plant Physiol.
Gallão,Maria I.; Cortelazzo,Angelo L.; Fevereiro,Manoel P. S.; Brito,Edy S. de.
Morphological changes and enzyme activity in suspension-cultured cells of Citrus aurantium were followed after chitin elicitation. Chitin oligomers presented a rapid effect with a maximum activity after 3 h followed by a gradual decrease to the original levels at 8 h of incubation. Cell walls presented a lignification process and the cytoplasmatic protein became less reactive to anionic stains. In the treated material a reduction in starch grain size, an increase in the number of autophagic vacuoles, deposition of secretion in the pericellular space and a defibrillation of the cell-wall polymers were observed. Chitin oligomers increased peroxidase and L-phenylalanine ammonia-lyase activities. Extracellular peroxidase activity increased from 0.20 U mL-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitin; Citrus aurantium; Elicitation; L-phenylalanine ammonia-lyase; Peroxidase.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100008
Registros recuperados: 19
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