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Co-culture of chondrocytes and bone marrow mesenchymal stem cells in vitro enhances the expression of cartilaginous extracellular matrix components BJMBR
Qing,Chang; Wei-ding,Cui; Wei-min,Fan.
Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed cells in cartilage tissue engineering. In the present study, we determined if the co-culture of rabbit articular chondrocytes and BMSCs in vitro promotes the expression of cartilaginous extracellular matrix and, if so, what is the optimal ratio of the two cell types. Cultures of rabbit articular chondrocytes and BMSCs were expanded in vitro and then cultured individually or at a chondrocyte:BMSC ratio of 4:1, 2:1, 1:1, 1:2, 1:4 for 21 days and cultured in DMEM/F12. BMSCs were cultured in chondrogenic induction medium. Quantitative real-time RT-PCR and Western blot were used to evaluate gene expression. In the co-cultures, type II collagen and aggrecan expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-culture; Bone mesenchymal stem cells; Chondrocytes; Tissue engineering.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400006
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Direct inhibitory effect of caffeine on viability, synthesis activity and gene expression in cultures of chondrocytes extracted from the articular cartilage of rats Arq. Bras. Med. Vet. Zootec.
Reis,A.M.S.; Oliveira,K.P.; Paula,I.H.F.; Silva,A.P.; Tarragô,J.F.; Ocarino,N.M.; Serakides,R..
ABSTRACT The aim of this study was to evaluate the effect of concentrations of caffeine on the viability, synthesis activity and gene expression in cultures of chondrocytes. Extracted articular cartilage from the femurs and tibias of 15 Wistar rats at three days old to isolate chondrocytes. Chondrocytes were cultured in chondrogenic medium (control) or supplemented with caffeine (0.5, 1.0, 2.0mM). Cell viability, alkaline phosphatase activity and collagen synthesis were assessed using colorimetric assays at 7, 14, 21 days. The chondrocyte cultures of all groups grown under coverslips were stained with hematoxylin-eosin to determine the percentage of cells/field and with PAS, safranin O, alcian blue to determine the percentage of matrix chondrogenic/field...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rats; Caffeine; Chondrocytes; Chondrogenesis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200509
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Enriching a cellulose hydrogel with a biologically active marine exopolysaccharide for cell-based cartilage engineering ArchiMer
Rederstorff, E.; Rethore, G.; Weiss, P.; Sourice, S.; Beck-cormier, S.; Mathieu, E.; Maillasson, M.; Jacques, Y.; Colliec-jouault, Sylvia; Fellah, B. H.; Guicheux, J.; Vinatier, C..
The development of biologically and mechanically competent hydrogels is a prerequisite in cartilage engineering. We recently demonstrated that a marine exopolysaccharide, GY785, stimulates the in vitro chondrogenesis of adipose stromal cells. In the present study, we thus hypothesized that enriching our silated hydroxypropyl methylcellulose hydrogel (Si-HPMC) with GY785 might offer new prospects in the development of scaffolds for cartilage regeneration. The interaction properties of GY785 with growth factors was tested by surface plasmon resonance (SPR). The biocompatibility of Si-HPMC/GY785 towards rabbit articular chondrocytes (RACs) and its ability to maintain and recover a chondrocytic phenotype were then evaluated in vitro by MTS assay, cell counting...
Tipo: Text Palavras-chave: Cartilage; Hydrogel; Glycosaminoglycan; Marine polysaccharides; Chondrocytes; Tissue engineering.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00303/41449/40650.pdf
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Insulin impairs the maturation of chondrocytes in vitro BJMBR
Torres,E.S.; Andrade,C.V.; Fonseca,E.C.; Mello,M.A.; Duarte,M.E.L..
The precise nature of hormones and growth factors directly responsible for cartilage maturation is still largely unclear. Since longitudinal bone growth occurs through endochondral bone formation, excess or deficiency of most hormones and growth factors strongly influences final adult height. The structure and composition of the cartilaginous extracellular matrix have a critical role in regulating the behavior of growth plate chondrocytes. Therefore, the maintenance of the three-dimensional cell-matrix interaction is necessary to study the influence of individual signaling molecules on chondrogenesis, cartilage maturation and calcification. To investigate the effects of insulin on both proliferation and induction of hypertrophy in chondrocytes in vitro we...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chondrocytes; Insulin; Micromass cultures; Apoptosis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900007
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Lentiviral-mediated RNAi targeting caspase-3 inhibits apoptosis induced by serum deprivation in rat endplate chondrocytes in vitro BJMBR
Ding,L.; Wu,J.P.; Xu,G.; Zhu,B.; Zeng,Q.M.; Li,D.F.; Lu,W..
Current studies find that degenerated cartilage endplates (CEP) of vertebrae, with fewer diffusion areas, decrease nutrient supply and accelerate intervertebral disc degeneration. Many more apoptotic cells have been identified in degenerated than in normal endplates, and may be responsible for the degenerated grade. Previous findings suggest that inhibition of apoptosis is one possible approach to improve disc regeneration. It is postulated that inhibition of CEP cell apoptosis may be responsible for the regeneration of endplates. Caspase-3, involved in the execution phase of apoptosis, is a candidate for regulating the apoptotic process. In the present study, CEP cells were incubated in 1% fetal bovine serum. Activated caspases were detected to identify...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cartilaginous endplate; Chondrocytes; RNA interference; Apoptosis; Caspase-3; Serum deprivation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600445
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Structure and secretory activity of cultured chondrocytes from patients with osteoarthritis Biocell
Montrull,Hilda L.; Brizuela,Nilda Y.; Demurtas,Silvia L.; Spitale,Luis; Meirovich,Carlos I..
Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco's modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO-2 and NO-3 were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA. The concentration of NO-x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml in the OA cartilage and 2,150 ± 190 ng/ml in the control samples. These increased values of MMP-1 and NO-x observed in the OA cartilage suggest a higher catabolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteoarthritis; Chondrocytes; Collagenase.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200002
Registros recuperados: 6
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