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Registros recuperados: 9
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Action of plant proteinase inhibitors on enzymes of physiopathological importance Anais da ABC (AABC)
Oliva,Maria Luiza V.; Sampaio,Misako U..
Obtained from leguminous seeds, various plant proteins inhibit animal proteinases, including human, and can be considered for the development of compounds with biological activity. Inhibitors from the Bowman-Birk and plant Kunitz-type family have been characterized by proteinase specificity, primary structure and reactive site. Our group mostly studies the genus Bauhinia, mainly the species bauhinioides, rufa, ungulata and variegata. In some species, more than one inhibitor was characterized, exhibiting different properties. Although proteins from this group share high structural similarity, they present differences in proteinase inhibition, explored in studies using diverse biological models.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bowman-Birk; Chymotrypsin; Kunitz inhibitors; Plasma kallikrein; Primary structure; Trypsin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300023
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Adaptation of digestive enzymes to dietary protein, carbohydrate and fibre levels and influence of protein and carbohydrate quality in Penaeus vannamei larvae (Crustacea, Decapoda) ArchiMer
Le Moullac, Gilles; Van Wormhoudt, A; Aquacop, Aquacop.
Activity of digestive enzymes (trypsin, chymotrypsin and amylase) was studied in relation with food in Penaeus vannamei larvae first stages. Microparticles containing casein involved the decrease of soluble protein content and enzyme activities. Specific trypsin activity but not chymotrypsin was correlated (p<1%) to casein level between 10 and 60% in microparticles. Cellulose fibres in food seemed to have little effect on digestive enzymes. Starch, between 1 and 20% in microparticles, had no influence on specific amylase activity. The effect of different feeding conditions were also tested on growth. No clear relationship between growth and enzyme is established since only trypsin is concerned in the case of a casein dose effect in the food. The protein...
Tipo: Text Palavras-chave: Amylase; Chymotrypsin; Trypsin; Digestive enzymes; Food; Larvae; Penaeus vannamei; Amylase; Chymotrypsine; Trypsine; Enzymes digestives; Alimentation; Larve; Penaeus vannamei.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-4431.pdf
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Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain Genet. Mol. Biol.
Gong,Jie; Xie,Yinjie; Yu,Kun; Yang,Ya'nan; Huang,Huiyang; Ye,Haihui.
Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chymotrypsin; Scylla paramamosain; Immune response; Immunocytochemistry; In situ hybridization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300010
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Chymotrypsin- and trypsin-like activities secreted by the multidrug-resistant yeasts forming the Candida haemulonii complex Anais da ABC (AABC)
SOUTO,XÊNIA M.; BRANQUINHA,MARTA H.; SANTOS,ANDRÉ L.S..
Abstract: Candida haemulonii complex (C. haemulonii, C. haemulonii var. vulnera and C. duobushaemulonii) consists of emergent multidrug-resistant pathogens that cause bloodstream and deep-seated infections. However, little is known about their virulence factors. Herein, we evaluated the presence of extracellular serine peptidases in this fungal complex. Serine peptidase activity was measured by spectrophotometry using chromogenic peptide substrates to the S1 family. Chymotrypsin-, trypsin- and elastase-like activities were detected in all fungal isolates. Since higher chymotrypsin- and trypsin-like activities were observed from the cleavage of N-succinyl-Ala-Ala-Pro-Phe-pNa and N-benzoyl-Phe-Val-Arg-pNa, respectively, these substrates were selected for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian clinical isolates; Candida haemulonii complex; Chymotrypsin; Serine peptidases; Trypsin; Virulence factors.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500803
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Composition of gastrointestinal content, protease and lipase activities in summer and winter of four freshwater siluriforms (Teleostei: Actinopterygii) with two different feeding habits Rev. Bras. Zool.
Almeida,Ana Paula Gottlieb; Zardo,Everton Luis; Toni,Candida; Behr,Everton Rodolfo; Silva,Leila Picolli da; Vieira,João Paes; Loro,Vania Lúcia; Baldisserotto,Bernardo.
ABSTRACT The aim of this study was to determine the composition of gastrointestinal content and protease and lipase activities in summer and winter as well as to evaluate the relationship between digestive enzyme activity and centesimal composition of gastrointestinal content and feeding habits of two omnivorous species, Rhamdia quelen (Quoy & Gaimard, 1824) and Pimelodus maculatus (LaCèpede, 1803), and of two detritivorous species, Loricariichthys anus (Valenciennes, 1835) and Hypostomus commersoni (Valenciennes, 1836). The activities of pepsin, trypsin, chymotrypsin, and lipase, and the levels of proteins and lipids in the gastrointestinal tract, were evaluated. The enzyme activities were not related to the centesimal composition of gastrointestinal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chymotrypsin; Fish; Lipase; Pepsin; Trypsin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100322
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Enzyme cleavable and biotinylated photoaffinity ligand with diazirine OAK
Hashimoto, Makoto; Okamoto, Shun’ji; Nabeta, Kensuke; Hatanaka, Yasumaru.
The efficient synthesis of an enzyme cleavable biotinylated diazirinyl photoaffinity ligand is described to allow the effective manipulation of the photolabeled biocomponents. The compound contains a glutamic acid gamma-methyl ester, which is a precursor of the substrate for V8 protease, between the diazirinyl photophor and biotin moiety. After alkaline hydrolysis of the ester, the compound can be proteolyzed at the Glu moiety by V8 protease. The photophore was introduced to L-Phe p-nitroanilide and the ligand was applied to photolabel of chymotrypsin to manipulate the application of the concept.
Palavras-chave: Photoaffinity labeling; Diazirine; Avidin-biotin; V8 protease; Chymotrypsin.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/738
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In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai J. Venom. Anim. Toxins incl. Trop. Dis.
Bae,Seong Kyeong; Lee,Hyunkyoung; Heo,Yunwi; Pyo,Min Jung; Choudhary,Indu; Han,Chang Hoon; Yoon,Won Duk; Kang,Changkeun; Kim,Euikyung.
Abstract Background: Because jellyfish are capable of provoking envenomation in humans, they are considered hazardous organisms. Although the effects of their toxins are a matter of concern, information on the venom components, biological activity and pathological mechanisms are still scarce. Therefore, the aim of the present study was to investigate a serine protease component of Nemopilema nomurai jellyfish venom (NnV) and unveil its characteristics. Methods: To determine the relationship between fibrinolytic activity of NnV and the serine protease, fibrin zymography was performed using metalloprotease and serine protease inhibitors. The biochemical characterization of serine proteases of NnV were determined by the amidolytic assay. Fractions with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nemopilema nomurai; Jellyfish venom; Chymotrypsin; Serine protease; Fibrinolytic activity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100323
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Role of Chicken Pancreatic Trypsin, Chymotrypsin and Elastase in the Excystation Process of Eimeria tenella Oocysts and Sporocysts OAK
Guyonnet, Vincent; Johnson, Joyce K.; Long, Peter L..
The role of pancreatic proteolytic enzymes in the excystation process of Eimeria tenella oocysts and sporocysts was studied in vitro. Intact sporulated oocysts were preincubated in phosphate buffer, NaCl 0.9% (PBS) added with 0.5% chicken bile extract in a 5% C02 atmosphere for 30 minutes prior to exposure to either 0.25% (w/v) chicken trypsin, chymotrypsin, pancreatic elastase, or a 1% (w/v) crude extract of unsporulated and sporulated oocysts of E. tenella (Expt.1). No excystation was observed under these conditions. Sporocysts were also incubated under the same conditions without pretreatment in C02. Excystation was observed for sporocysts incubated with either trypsin, chymotrypsin or pancreatic elastase, the best percentage of excystation being...
Palavras-chave: Chymotrypsin; Eimeria tenella; Elastase; Excystation; Trypsin.
Ano: 1991 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/155
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Variations des activites digestives en fonction des facteurs du milieu chez les crustaces ArchiMer
Galgani, Francois; Benyamin, Y; Vanworhmoudt, A; Cecaldi, J.
Proteolytic enzymes of hepatopancreas of six species of peneids (Penaeus kerathurus, P. japonicus, P. monodon, P. stylirostris, P. merguiensis and P. vannamei ) have been studied. Some of their physiological variations have been characterised. Increase in acclimation temperature of crustaceans enhances increase of specific activities of proteases. Variations of salinity does not modify specific activities, nor quantities of trypsin, nor electrophoretic patterns of these different proteases.
Tipo: Text Palavras-chave: Penaeus kerathurus; Penaeidae; Malacostraca; Diets; Growth; Larval development; Life cycle; Acclimation; Environmental effects; Digestive glands; Enzymatic activity; Salinity effects; Temperature effects; Enzymes; Endocrinology; Digestion; Développement larvaire; Croissance; Cycle d'intermue; Carboxypeptidase A and B; Leucine aminopeptidase; Chymotrypsin; Trypsine; Enzymes digestives; Homarus ganmarus; Palaemon serratus; P. vannamei; P. merguiensis; P. stylirostris; P. monodon; P. japonicus; Penaeus keratkurus.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1195.pdf
Registros recuperados: 9
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