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Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses Scientia Agricola
Arena,Gabriella Dias; Ramos-González,Pedro Luis; Nunes,Maria Andréia; Jesus,Camila Chabi; Calegario,Renata Faier; Kitajima,Elliot Watanabe; Novelli,Valdenice Moreira; Freitas-Astúa,Juliana.
ABSTRACT: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clerodendrum chlorotic spot virus; Cilevirus; Solanum violaefolium ringspot virus; Coffee ringspot virus; Dichorhavirus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000100085
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In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). Repositório Alice
CALEGARIO, R. F.; LABATE, M.T.V.; PERONI, LUÍS A.; STACH-MACHADO, D. R.; ASTUA, J. de F.; LABATE, C. A.; MACHADO, M. A.; KITAJIMA, E. W..
Citrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus; Citrus leprosis virus C; Brevipalpus phoenicis; Cilevirus; Citrus sinensis; Polyclonal antibody; Serology.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937928
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