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Molecular authentication of Pargo fillets Lutjanus purpureus (Perciformes: Lutjanidae) by DNA barcoding reveals commercial fraud Neotropical Ichthyology
Veneza,Ivana; Silva,Raimundo; Freitas,Leilane; Silva,Sâmia; Martins,Kely; Sampaio,Iracilda; Schneider,Horacio; Gomes,Grazielle.
ABSTRACT The Caribbean Red Snapper (Pargo) Lutjanus purpureus is the most economically important snapper in Brazil, which is sold, among other forms, as frozen fillets. During the process of transformation into fillets there is the removal of the distinctive morphological traits, being able to favor the substitution by less valued species. In addition, there is no national legislation requiring the insertion of the specific name on the product label. However, according to a Normative Instruction (IN N ° 29/2015 MAPA) that correlates the common and specific names of the products destined to the national trade, in Brazil only L. purpureus and L. campechanus can be denominated “Pargo”. Thus, the DNA barcode tool was used to identify the fillets sold in north...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Commercial fraud; DNA barcode; Fillets; Processed fish; Snapper.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100211
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Molecular detection of adulteration in commercial buffalo meat products by multiplex PCR assay Ciênc. Tecnol. Aliment.
WANG,Lanping; HANG,Xinru; GENG,Rongqing.
Abstract Authentication of commercial buffalo meat products has become a market concern. This study intended to develop and validate a highly species-specific multiplex PCR assay for authentication of buffalo meat products. Four pairs of species-specific primers were used to target mitochondrial cytochrome oxidase I (COI) gene. The assay generated the expected PCR products of 313, 255, 294 and 177 bp for buffalo meat, cattle meat, pork meat and duck meat, respectively. The multiplex PCR assay was sensitive enough to detect 1 pg pure DNA and 0.1% (w/w) adulterated meat under mixed matrices. Market survey revealed about 35.3% of buffalo meat products are adulterated with cattle meat, pork meat or duck meat in China. The adulteration was found in all food...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo meat; Commercial fraud; Adulteration; Multiplex PCR; Cytochrome oxidase I gene.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000200344
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