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| FARI, M.; RESENDE, G. M. de; MELO, N. F. de. |
| Este trabalho teve por objetivo avaliar a capacidade de regeneracao das cultivares de tomateiro industrial (Lycopersicon esculentum Mill) IPA-5 e IPA-6, utilizando quatro composicoes de meio de cultura descritos na literatura e cinco variacoes de inoculacao. Foi testada uma nova variacao de inoculacao, denominada cotiledone fendido. A maior frequencia de formacao de gemas vegetativas foi 100% no caso de IPA-5, e 65% no caso de IPA-6. Para induzir o alongamento de brotos, foram necessarios tres subcultivos dos explantes apresentando gemas. No caso de IPA-5, o numero de brotos obtidos foi maior quando a inducao de gemas foi realizada em meio contendo BAP e AIA seguido de tres subcultivos, em meio como zeatina. Usando esse protocolo, a cultivar IPA-5 produziu... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Tomate; Variedade; Organogenese; Cultura de tecido; Explante; Cotiledone; Transformacao genetica; Lycopersicon esculentum; Tomatoes; Varieties; Organogenesis; Tissue culture; Cotyledons; Explants; Genetic transformation. |
| Ano: 2000 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/134062 |
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| DANTE, R. A.; LARKINS, B. A.; SABELLI, P. A.. |
| Seed development is a complex process that requires coordinated integration of many genetic, metabolic, and physiological pathways and environmental cues. Different cell cycle types, such as asymmetric cell division, acytokinetic mitosis, mitotic cell division, and endoreduplication, frequently occur in sequential yet overlapping manner during the development of the embryo and the endosperm, seed structures that are both products of double fertilization. Asymmetric cell divisions in the embryo generate polarized daughter cells with different cell fates. While nuclear and cell division cycles play a key role in determining final seed cell numbers, endoreduplication is often associated with processes such as cell enlargement and accumulation of storage... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Ciclo celular; Desenvolvimento de semente; Cyclin-dependent kinase; Endoreduplication; Retinoblastoma-related; Seed coat; Endosperma; Cell division; Cotyledons; Embryo (plant); Endopolyploidy; Endosperm; Testa. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1031346 |
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| Aidar,M.P.M.; Martinez,C.A.; Costa,A.C.; Costa,P.M.F.; Dietrich,S.M.C.; Buckeridge,M.S.. |
| Plants grown in elevated CO2 environments may exhibit photosynthetic acclimation or down regulation, which is characterised by reduced rates of photosynthesis. In most cases of CO2-induced photosynthetic acclimation, the reduced rates of photosynthesis were still higher than those detected in plants growing at ambient CO2 concentrations. In this work we present a study on the behaviour of seedlings of Hymenaea courbaril, a late secondary/climax species that is one of the most important trees in mature tropical forests of the Americas. After germination, the seedling of H. courbaril increases its rate of growth due to the mobilisation of massive amounts of a storage cell wall polysaccharide (xyloglucan) from its cotyledons. In our experiments, germinated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Photosynthesis; CO2 enrichment; Hymenaea courbaril; Storage mobilisation; Root:shoot ratio; Seedling growth; Cotyledons; Open top chamber; Xyloglucan; Biodiversity. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032002000100008 |
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| ENÉAS-FILHO,JOAQUIM; BARBOSA,GISLAINY KARLA DA COSTA; SUDÉRIO,FABRÍCIO BONFIM; PRISCO,JOSÉ TARQUÍNIO; GOMES-FILHO,ENÉAS. |
| Three isoforms of beta-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of Vita 3 and Vita 5 cowpea [Vigna unguiculata (L.) Walp.] cultivars differing in water and salt stress tolerance. The purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through DEAE-sephadex and affinity chromatography through Lactosyl-sepharose columns. The three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60ºC), identical thermal stability up to 50°C, and similar pH optima (3-4). However, they differed from each other in sensitivity towards metal ions and certain chemical agents... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cotyledons; Isozymes; Vigna unguiculata; Vita 3 and Vita 5 cultivars; Salt tolerance. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300001 |
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| ENÉAS-FILHO,JOAQUIM; SUDÉRIO,FABRÍCIO BONFIM; GOMES-FILHO,ENÉAS; PRISCO,JOSÉ TARQUÍNIO. |
| Cotyledonary b-galactosidases were isolated and partially purified from Pitiúba cowpea (Vigna unguiculata (L.) Walp.) quiescent seeds. The purification steps consisted of precipitation of the crude extract with ammonium sulphate in the range of 20-60% saturation, acid precipitation, DEAE-Sephadex ion-exchange chromatography and Lactosyl-Sepharose affinity chromatography. This purification process gave rise to three b-galactosidases-rich fractions: b-gal I, b-gal II and b-gal III, which were purified about 5, 509, and 62 fold, respectively. They reached maximal enzyme activity at different pH ranges: 3.5-4.5 for b-gal I, 3.0-3.5 for b-gal II, and 3.0-4.0 for b-gal III. Their maximal activities were reached when the temperature of the assay medium was 60° C,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cotyledons; Cowpea; Enzyme purification; Quiescent seeds. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042000000100008 |
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| Dos Santos Silveira,Conceição Eneida; Palhares,Dario; Barros,Karina; Pereira,Damasceno; Gomes Luís,Zanderluce; Rodrigues Pereira,Luiz Alfredo. |
| ABSTRACT Byrsonima basiloba Juss. is a fast growing shrub that produces edible and juicy fruits. This research studied the morphology and anatomy of seeds and seedlings. The hard endocarp was crested and triloculate, with one seed per locule. B. basiloba seed was composed of a testa and a tegmen with residual endosperm. The embryo was ivory, with long, foliaceous, circinate, anisophyllous cotyledons with disjointed apices. The embryonary axis was short, straight and occupied a small portion of the seed with short epicotyl, and radicle with a rudimentary root cap. In the seedlings, it was possible to differentiate the protoderm, procambium and ground meristem in the elongation zone. The differentiation process in this species occurred early in the embryo.... |
| Tipo: Journal article |
Palavras-chave: Seedling; Cotyledons; Endocarp; Amphistomy; Cerrado. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000200355 |
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