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Registros recuperados: 15
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Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration Neotropical Entomology
Fermino,Fábio; Conte,Hélio; Falco,José R P.
Diatraea saccharalis (Fabricius) has cryptonephridial type Malphigian tubules (MT). This type of MT is characterized by the penetration of the distal part of the MT into the external walls of the rectum, which is usually lined with a perinephral membrane. The MT is divided into three differentiated regions: proximal, middle and distal. In this study, our objective was to compare the nuclear activities of each one of the three regions of the D. saccharalis MT by using a nuclear basophilic technique and critical electrolyte concentration with a toluidine blue stain at pH 4.0. This method allows differentiation of DNA/protein complexes in in situ and in vitro chromatin. MT chromatin structure in D. saccharalis is variable. Fifth instars have a more...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochemistry; Larval development; Cryptonephridial system.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000400016
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Cytochemical aspects of the peripheral blood cells of Oreochromis (Tilapia) niloticus. (Linnaeus, 1758) (Cichlidae, Teleostei): part II Braz. J. Vet. Res. Anim. Sci.
UEDA,Ivete Kotomi; EGAMI,Mizue Imoto; SASSO,Wilson da Silva; MATUSHIMA,Eliana Reiko.
Morphologicaly, seven types of cells were identified in the blood of Oreochromis niloticus: erythrocytes, thrombocytes, neutrophils, eosinophils, basophils, lymphocytes and monocytes. Glycogen was present not only in the cytoplasm of neutrophils and thrombocytes but also in some lymphocytes and monocytes. The positive reaction for myeloperoxidase and Sudan black was observed in neutrophils and eosinophils. The bromphenol blue method was strongly positive for erythrocytes and eosinophils.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood cells; Cytochemistry; Oreochromis niloticus; Fishes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000600005
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Distribution of pectins in the pollen apertures of Oenothera hookeri.velans ster/+ster: Biocell
Noher de Halac,I.; Cismondi,I.A.; Rodriguez-Garcia,M.I.; Famá,G..
Cell wall pectins are some of the most complex biopolymers known, and yet their functions remain largely mysterious. The aim of this paper was to deepen the study of the spatial pattern of pectin distribution in the aperture of Oenothera hookeri.velans ster/+ster fertile pollen. We used "in situ" immunocytochemical techniques at electron microscopy, involving monoclonal antibodies JIM5 and JIM7 directed against pectin epitopes in fertile pollen grains of Oenothera hookeri.velans ster/+ster. The same region was also analyzed by classical cytochemistry for polysaccharide detection. Immunogold labelling at the JIM7 epitope showed only in mature pollen labelling mainly located at the intine endo-aperture region. Cytoplasmic structures near the plasma membrane...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectins; Monoclonal antibodies; JIM5; JIM7; Immunogold; Pollen; Sporoderm; Oenothera; Cytochemistry.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000100002
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Electron microscopy of glial cells of the central nervous system in the crab Ucides cordatus BJMBR
Allodi,S.; Taffarel,M..
Invertebrate glial cells show a variety of morphologies depending on species and location. They have been classified according to relatively general morphological or functional criteria and also to their location. The present study was carried out to characterize the organization of glial cells and their processes in the zona fasciculata and in the protocerebral tract of the crab Ucides cordatus. We performed routine and cytochemical procedures for electron microscopy analysis. Semithin sections were observed at the light microscope. The Thiéry procedure indicated the presence of carbohydrates, particularly glycogen, in tissue and in cells. To better visualize the axonal ensheathment at the ultrastructural level, we employed a method to enhance the...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Glial cells; Crustaceans; Electron microscopy; Cytochemistry; Ucides cordatus.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300013
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MORPHO-CYTOCHEMICAL AND ULTRASTRUCTURAL CHARACYERIZATION OF PERIPERAL THROMBOCYTES OF ROADSIDE HAWK Buteo magnirostris GMELIN, 1788) (AVIAN, FALCONIFORM) International Journal of Morphology
Aguiar Santos,Antenor; Marques Joppert da Silva,Adriana; Lima de Carvalho,Vinicius Antonio; Imoto Egami,Mizue.
Basic hematological information about the avian in its natural habitat is limited. In this study we aimed at investigating, in the peripherical blood of the roadside hawk, Buteo magnirostris, the number of thrombocytes and their morphological, cytochemical and ultrastructural aspects. Blood smears were submited to the following methods: Leishman for morphological analysis and specific methods for the characterization of four kinds of macromolecules of different chemical nature: Glycogen, lysosomal enzymes (myeloperoxidase and the acid phosphatase), basic polyaminoacid rich proteins and phospholipids. The average number of thrombocytes was 26.8 x 10³ ± 10,2 / µL. Structurally the thrombocytes present predominantly elliptic shape, with...
Tipo: Journal article Palavras-chave: Avian; Thrombocyte; Morphology; Cytochemistry; Ultrastructure.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400004
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Morphogenese sexuelle et regulation hormonale de l'activite genitale chez la crevette Penaeus japonicus en elevage ArchiMer
Laubier, A; Chim, Liet; Payen, G.
A study of the sexual physiology of penaeid prawns essentially based upon Penaeus japonicus is performed in both descriptive and experimental ways, using the controlled rearings conducted at the Centre Oceanologique de Bretagne (CNEXO-COB), Brest. Sexual morphogenesis is described. This preliminary study enables definition of simple criteria for determination of the stage of genital development of the prawns during their growth. The neuroendocrine control of the genital system development is studied, using eyestalks ablations and male eyestalks extracts injection in prawns belonging to the same sex. A biochemical criterion as a test of the effect of eyestalks extract injections on the genital system development is discussed. The evolution of the specific...
Tipo: Text Palavras-chave: Penaeus japonicus; Malacostraca; Controlled conditions; Neurosecretion; Cytochemistry; Ultrastructure; Sexual reproduction; Sexual maturity; Sexual dimorphism; Endocrinology; Eyestalk extirpation; Gametogenesis; Sex determination; Morphogenesis; Contrôle neuroendocrine de la reproduction; Morphogénèse sexuelle; Physiologie sexuelle; Élevage contrôlé; Penaeus japonicus.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1188.pdf
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Purification de peptides apparentes a la calcitonine de saumon chez les crustaces. Resultats preliminaires ArchiMer
Vanwormhoudt, A.
In crustacean, immunoreactive peptides were detected with salmon-calcitonin antibodies by immunocytochemistry in its eyestalks and by radioimmunoassay in the haemolymph and the digestive tract. Two different molecules were purified: the first with their molecular weight comprised between 3-5 kDa, the second having a molecular weight of 22 kDa which could correspond to a precursor of the first one. Their characterization is on progress.
Tipo: Text Palavras-chave: Crustacea; Biochemistry; Cytochemistry; Hormones.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-1385.pdf
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Quelques aspects de la regulation du systeme neuroendocrine produisant la CHH et de la relation entre le rythme circadien et la glycemie ArchiMer
Kallen, J.
In crayfish, the hemolymph glucose level shows an endogenous circadian rhythmicity which is expressed by a nocturnal glucose rise. This study shows that injections of serotonin and dopamine result in hyperglycemia and that injections of met-enkephalin decrease hemolymph glucose level as well as the nocturnal circadian glucose peak. Using immunocytochemistry, these neurotransmitters have been located in the eyestalk and their relationship with the CHH cell system is described.
Tipo: Text Palavras-chave: Orconectes limosus; Astacus leptodactylus; Malacostraca; Cytochemistry; Hormones; Neurotransmitters; Circadian rhythms; Glucose.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-1383.pdf
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Reações citoquímicas das células sanguíneas de tuvira (Gynmotus sp.). Repositório Alice
VENTURA, A. S.; SANTOS, J. S. dos; PÁDUA, S. B.; OLIVEIRA, D. B. de; PEREIRA, E. S.; ISHIKAWA, M. M..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Eritrócitos; Trombócitos; Gynmotus sp; Citoquímica; Cytochemistry; Fish culture.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010675
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Seed reserve composition and mobilization during germination and early seedling establishment of Cereus jamacaru D.C. ssp. jamacaru (Cactaceae) Anais da ABC (AABC)
Alencar,Nara L.M.; Innecco,Renato; Gomes-Filho,Enéas; Gallão,Maria Izabel; Alvarez-Pizarro,Juan C.; Prisco,José T.; Oliveira,Alexandre B. De.
Cereus jamacaru, a Cactaceae found throughout northeast Brazil, is widely used as cattle food and as an ornamental and medicinal plant. However, there has been little information about the physiological and biochemical aspects involved in its germination. The aim of this study was to investigate its reserve mobilization during germination and early seedling growth. For this, C. jamacaru seeds were germinated in a growth chamber and collected at 0, 2, 4, 5, 6, 8 and 12 days after imbibition for morphological and biochemical analyses. Dry seeds had wrinkled seed coats and large, curved embryos. Lipids were the most abundant reserve, comprising approximately 55% and 65% of the dry mass for cotyledons and the hypocotylradicle axis, respectively. Soluble sugars...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cactaceae; Carbohydrates; Cytochemistry; Lipids; Morphology; Proteins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300024
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Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): cytochemical, structural and ultrastructural characterization Genet. Mol. Biol.
Rego,Letícia do Nascimento Andrade de Almeida; Silistino-Souza,Rosana; Azeredo-Oliveira,Maria Tercília Vilela de; Madi-Ravazzi,Lilian.
Zaprionus indianus is a drosophilid native to the Afrotropical region that has colonized South America and exhibits a wide geographical distribution. In contrast, Z. sepsoides is restricted to certain African regions. The two species differ in the size of their testes, which are larger in Z. indianus than in Z. sepsoides. To better understand the biology and the degree of differentiation of these species, the current study evaluated spermatogenesis in males of different ages by conventional staining techniques and ultrastructural analysis. Spermatogenesis and the ultrastructure of spermatozoa were similar in the two species, and the diploid number was confirmed to be 2n = 12. A greater number of spermatozoa were observed in young Z. indianus (1-3 days old)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zaprionus indianus; Zaprionus sepsoides; Spermatogenesis; Cytochemistry; Ultrastructure; Meiosis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100008
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Structural, hystochemical and cytochemical characteristics of the stigma and style in Passiflora edulis f. flavicarpa (Passifloraceae) BABT
Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Dias,Angelo José Burla; Ribeiro,Beatriz Ferreira; Viana,Alexandre Pio.
Studies were carried out on anatomic aspects of the stigma and style of yellow passionfruit. The stigmatic style consisted of papillae, many layers of sub-epidermal cells and a central transmitting tissue. The stigma was of dry, unbranched, multicellular multiseriate papillate type and the cells were highly vacuolated with walls that were relatively thin consisting of compactly arranged fibrils. The style was solid with a central core of transmitting tissue that had intercellular spaces containing a matrix that included polysaccharidic and lipidic substances. During development, cells of the transmitting tissue were arranged compactly while the mature pistil showed cells with a little more loosely arranged intercellular spaces. Both the mature stigma and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yellow passionfruit; Anatomy; Stigma and style; Histochemistry; Cytochemistry.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100011
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The surface charge of trypanosomatids Anais da ABC (AABC)
SOUTO-PADRÓN,THAÏS.
The surface charge of trypanosomatids was evaluated by means of the binding of cationic particles, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility of cells. The results obtained indicate that most of the trypanosomatids exhibit a negatively charged surface whose value is species specific and varies according to the developmental stages. Sialic acids associated with glycoproteins, glycolipids and phosphate groups are the major components responsible for the net negative surface charge of the trypanosomatids.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosomatids; Cell surface charge; Electrophoretic mobility; Cytochemistry.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400007
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Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators Agronomy
Santos, Dalilhia Nazaré dos; Nunes, Claudinéia Ferreira; Soares, Joyce Dória Rodrigues; Alves, Eduardo; Labory, Cláudia Regina Contijo; Pasqual, Moacir; Pio, Leila Aparecida Salles.
This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical behavior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 µM 2,4-D; (3) 9.04 µM 2,4-D; (4) 4.14 µM PIC; (5) 8.28 µM PIC; (6) 4.52 µM 2,4-D + 2.32 µM KIN; (7) 9.04 µM 2,4-D + 4.64 µM KIN; (8) 4.14 µM PIC + 2.32 µM KIN; (9) 8.28 µM PIC + 4.64 µM KIN; (10) 4.52 µM 2,4-D + 2.22 µM BAP; (11) 9.04 µM 2,4-D + 4.44 µM BAP; (12) 4.14 µM PIC + 2.22 µM BAP and (13) 8.28 µM PIC + 4.44 µM BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed scanning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jatropha curcas L.; Embryogenesis; Microscopy; Cytochemistry.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19745
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Ultrastructural detection of lipids in the cephalic salivary glands of Apis mellifera and Scaptotrigona postica (Hymenoptera: Apidae) workers Rev. Bras. Zool.
Poiani,Silvana Beani; Cruz-Landim,Carminda da.
ABSTRACT Secretory cells of the cephalic salivary glands (CSGs) of eusocial bees produce and accumulate lipid-like secretion in the lumens of their alveoli. Correspondingly, secretory cells present typical ultrastructural features of lipid-compound producers. Previous work on bees has revealed inter-specific differences in the chemical composition of secretion, and the production mechanisms and secretory cycle of secretory cells. In this work a comparative analysis of the mechanisms of lipid storage in the CSGs of Apis mellifera (Linnaeus, 1758) and Scaptotrigona postica (Latreille, 1807) workers was carried out. The ultrastructural location of lipids was ascertained using imidazole-osmium (IO), using individuals in different stages of their life cycles....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochemistry; Excretory; Imidazole-osmium; Insect; Salivary system.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702016000300901
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