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Registros recuperados: 4
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A search for RNA insertions and NS3 gene duplication in the genome of cytopathic isolates of bovine viral diarrhea virus BJMBR
Quadros,V.L.; Mayer,S.V.; Vogel,F.S.F.; Weiblen,R.; Brum,M.C.S.; Arenhart,S.; Flores,E.F..
Calves born persistently infected with non-cytopathic bovine viral diarrhea virus (ncpBVDV) frequently develop a fatal gastroenteric illness called mucosal disease. Both the original virus (ncpBVDV) and an antigenically identical but cytopathic virus (cpBVDV) can be isolated from animals affected by mucosal disease. Cytopathic BVDVs originate from their ncp counterparts by diverse genetic mechanisms, all leading to the expression of the non-structural polypeptide NS3 as a discrete protein. In contrast, ncpBVDVs express only the large precursor polypeptide, NS2-3, which contains the NS3 sequence within its carboxy-terminal half. We report here the investigation of the mechanism leading to NS3 expression in 41 cpBVDV isolates. An RT-PCR strategy was employed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine viral diarrhea virus; CpBVDV; NS3 gene; Cytopathology; RNA processing.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700012
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Differences in intracellular localization of corn stunt spiroplasmas in magnesium treated maize. Repositório Alice
NOME, C.; MAGALHAES, P. C.; OLIVEIRA, E.; NOME, S.; LAGUNA, I. G..
Maize plants infected with Spiroplasma kunkelii show symptoms similar to that of plants in a magnesium-deficient soil, and it has been shown that the spiroplasma alters the plants' magnesium absorption. In the current study we compared changes associated to either spiroplasma infection, two soil magnesium levels and their combinations. Plant symptoms were recorded and correlated with transmission electron microscopy observations. Plants grown on a high magnesium treatment showed no macroscopical alterations nor organelle ultrastructural alterations, while plants on a low magnesium treatment showed macroscopical vein yellowing and, ultrastructurally, they had most chloroplasts and mitochondrial membranes altered. Infected plants on a low magnesium treatment...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mollicutes; Cytopathology; Plant pathology; Magnesium deficiency.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/859089
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Differences in intracellular localization of corn stunt spiroplasmas in magnesium treated maize Biocell
Nome,Claudia; Magalhães,Paulo César; Oliveira,Elizabeth; Nome,Sergio; Laguna,Irma Graciela.
Maize plants infected with Spiroplasma kunkelii show symptoms similar to that of plants in a magnesium-deficient soil, and it has been shown that the spiroplasma alters the plants' magnesium absorption. In the current study we compared changes associated to either spiroplasma infection, two soil magnesium levels and their combinations. Plant symptoms were recorded and correlated with transmission electron microscopy observations. Plants grown on a high magnesium treatment showed no macroscopical alterations nor organelle ultrastructural alterations, while plants on a low magnesium treatment showed macroscopical vein yellowing and, ultrastructurally, they had most chloroplasts and mitochondrial membranes altered. Infected plants on a low magnesium treatment...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Mollicutes; Cytopathology; Plant pathogen; Magnesium deficiency.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200008
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The in vitro comparative cytopathology of a porcine rotavirus and the simian prototype (SA-11) Arq. Bras. Med. Vet. Zootec.
Lonien,S.C.H.; Bolognini,A.M.; Linhares,R.E.C.; Nozawa,C.M..
A citopatologia in vitro de uma cepa de rotavírus porcino adaptado em cultura de células foi comparada à estirpe-protótipo símia (SA-11). O efeito citopático (ECP) produzido pelos vírus foi semelhante embora a estirpe porcina tivesse apresentado algumas alterações diferentes, como o acentuado estreitamento do citoplasma, com grande perda do volume citoplasmático. O vírus porcino apresentou menor número de plaques de ECP porém com diâmetro maior em relação ao vírus símio, demonstrando maior capacidade de disseminação célula-célula, quase oito vezes mais, a julgar pelo diâmetro dos plaques de ECP. Os elementos do citoesqueleto das células infectadas revelaram uma reorganização semelhante para ambas as estirpes, não sendo possível observar nenhuma diferença,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Rotavirus; Cytopathology; Cell culture; Plaque; Cytoskeleton.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400002
Registros recuperados: 4
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