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A simple and effective pressure culture system modified from a transwell cell culture system Biol. Res.
Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian.
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is...
Tipo: Journal article Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007
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Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation BJMBR
Mermelstein,C.S.; Rebello,M.I.L.; Amaral,L.M.; Costa,M.L..
Although much is known about the molecules involved in extracellular Ca2+ regulation, the relationship of the ion with overall cell morphology is not understood. The objective of the present study was to determine the effect of the Ca2+ chelator EGTA on the major cytoskeleton components, at integrin-containing adhesion sites, and their consequences on cell shape. Control mouse cell line C2C12 has a well-spread morphology with long stress fibers running in many different directions, as detected by fluorescence microscopy using rhodamine-phalloidin. In contrast, cells treated with EGTA (1.75 mM in culture medium) for 24 h became bipolar and showed less stress fibers running in one major direction. The adhesion plaque protein alpha5-integrin was detected by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytoskeleton; Extracellular Ca2+; Cell shape; Microfilaments; Microtubules; Integrin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800018
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Cytoskeletal and cellular adhesion proteins in zebrafish (Danio rerio) myogenesis BJMBR
Costa,M.L.; Escaleira,R.; Manasfi,M.; Souza,L.F. de; Mermelstein,C.S..
The current myogenesis and myofibrillogenesis model has been based mostly on in vitro cell culture studies, and, to a lesser extent, on in situ studies in avian and mammalian embryos. While the more isolated artificial conditions of cells in culture permitted careful structural analysis, the actual in situ cellular structures have not been described in detail because the embryos are more difficult to section and manipulate. To overcome these difficulties, we used the optically clear and easy to handle embryos of the zebrafish Danio rerio. We monitored the expression of cytoskeletal and cell-adhesion proteins (actin, myosin, desmin, alpha-actinin, troponin, titin, vimentin and vinculin) using immunofluorescence microscopy and video-enhanced,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytoskeleton; Cell adhesion; Zebrafish; Myogenesis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800019
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Dexamethasone blocks the migration of the human neuroblastoma cell line SK-N-SH BJMBR
Casulari,L.A.; Dondi,D.; Maggi,R.; Demissie,M.; Piccolella,M.; Piva,F..
Glucocorticoids (Gc) influence the differentiation of neural crest-derived cells such as those composing sympathoadrenal tumors like pheochromocytomas, as well as neuroblastomas and gangliomas. In order to obtain further information on the effects of Gc on cells evolving from the neural crest, we have used the human neuroblastoma cell line SK-N-SH to analyze: 1) the presence and the binding characteristics of Gc receptors in these cells, 2) the effect of dexamethasone (Dex) on the migration of SK-N-SH cells, and 3) the effect of Dex on the organization of the cytoskeleton of SK-N-SH cells. We show that: 1) receptors that bind [³H]-Dex with high affinity and high capacity (Kd of 9.6 nM, Bmax of 47 fmol/mg cytosolic protein, corresponding to 28,303...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human neuroblastoma; SK-N-SH cells; Glucocorticoids; Migration; Cytoskeleton.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900011
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Hypothyroidism decreases proinsulin gene expression and the attachment of its mRNA and eEF1A protein to the actin cytoskeleton of INS-1E cells BJMBR
Goulart-Silva,F.; Serrano-Nascimento,C.; Nunes,M.T..
The actions of thyroid hormone (TH) on pancreatic beta cells have not been thoroughly explored, with current knowledge being limited to the modulation of insulin secretion in response to glucose, and beta cell viability by regulation of pro-mitotic and pro-apoptotic factors. Therefore, the effects of TH on proinsulin gene expression are not known. This led us to measure: a) proinsulin mRNA expression, b) proinsulin transcripts and eEF1A protein binding to the actin cytoskeleton, c) actin cytoskeleton arrangement, and d) proinsulin mRNA poly(A) tail length modulation in INS-1E cells cultured in different media containing: i) normal fetal bovine serum - FBS (control); ii) normal FBS plus 1 µM or 10 nM T3, for 12 h, and iii) FBS depleted of TH for 24 h (Tx)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proinsulin mRNA; EEF1A protein; Cytoskeleton; Hypothyroidism; INS-1E cells.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000012
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Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi IPB - Escola Superior Agrária
Jorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Ángel; Choupina, Altino.
Actins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural proteins, found in all eukaryotes. So, actin gene sequences are used as tools in scientific research, for example, for phylogenetic analysis. Actin in Phytophthora infestans is encoded by at least two genes, in contrast to unicellular and filamentous fungi (Candida albicans, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis and...
Tipo: Article Palavras-chave: Phytophthora cinnamomi; Ink disease; Cytoskeleton; Actin.
Ano: 2010 URL: http://hdl.handle.net/10198/3389
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Isolation and phylogenetic analysis of two actin genes from Phytophthora cinnamomi IPB - Escola Superior Agrária
Jorge, Lurdes; Dias, Teresa; Andrade, Maria; Vaz, Madalena; Dominguez, Ángel; Choupina, Altino.
Actins, as the essential component of cellular microfilament, are ubiquitous and highly conserved proteins that play key roles in several basic functions of organism such as cytoskeleton morphology, cell division, cell motility, cellular signal transduction, cellular interaction and organelle movements, as well as locomotion, phagocytosis, endocytosis and exocytosis . Actins are highly conserved structural proteins, found in all eukaryotes. So, actin gene sequences are used as tools in scientific research, for example, for phylogenetic analysis. Actin in Phytophthora infestans is encoded by at least two genes, in contrast to unicellular and filamentous fungi (Candida albicans, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis and...
Tipo: ConferenceObject Palavras-chave: Phytophthora cinnamomi; Ink disease; Cytoskeleton; Actin.
Ano: 2010 URL: http://hdl.handle.net/10198/4261
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Morphology and histochemistry of glandular trichomes in Hyptis villosa Pohl ex Benth. (Lamiaceae) and differential labeling of cytoskeletal elements Acta Botanica
Tozin,Luiz Ricardo dos Santos; Rodrigues,Tatiane Maria.
ABSTRACT Lamiaceae contains many species known for their aromatic properties that are produced by the production of essential oils in glandular trichomes. Hyptis is one of the most common genera of Lamiaceae in the Brazilian flora, and includes several species with medicinal value. However, studies on the morphology and functioning of their glandular trichomes are lacking. We analyzed the morphology, histochemistry and ultrastructure of the glandular trichomes in leaves of H. villosa, emphasizing the differential distribution of actin filaments and microtubules in cells secreting hydrophilic and lipophilic compounds. Four morphotypes of glandular trichomes were identified. Total lipid, terpenes, alkaloids, phenolic compounds, proteins and polysaccharides...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actin microfilaments; Cytoskeleton; Glandular hairs; Microtubules; Ultrastructure.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300330
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Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells Biol. Res.
Xu,Huiyun; Zhang,Jian; Wu,Jiawei; Guan,Ying; Weng,Yuanyuan; Shang,Peng.
Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of oscillatory fluid flow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a significant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin avp3. However,...
Tipo: Journal article Palavras-chave: Cytoskeleton; Fluid flow stress; Integrin-associated molecules; MC3T3-E1 osteoblast-like cell line; MLO-Y4 osteocyte-like cell line.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200008
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Recent advances in aluminum toxicity and resistance in higher plants Braz. J. Plant Physiol.
Vitorello,Victor Alexandre; Capaldi,Flávia Regina; Stefanuto,Vanderlei Antonio.
Aluminum toxicity is a major soil constraint to food and biomass production throughout the world. Considerable advances in the understanding of the mechanism of resistance involving exudation of organic acids have been made in recent years. However, despite intense research efforts, there are many aspects of Al toxicity that remain unclear. This article reviews the features of the chemistry of Al relevant to its toxicity followed by an examination of the mechanisms of toxicity and resistance. Emphasis, however, is given to the mechanisms of Al toxicity, since resistance has been covered recently by several reviews. Some topics which are specifically discussed in this review are: a) The possible role of cellular effects of low pH in Al toxicity, which has...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Al uptake and localization; Cell wall; Cytoskeleton; Differential sensitivity to Al; Low pH; Acidity; Plasma membrane.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100011
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Solution blow spinning fibres: new immunologically inert substrates for the analysis of cell adhesion and motility. Repositório Alice
PASCHOALIN, R. T.; TRALDI, B.; AYDIN, G.; OLIVEIRA, J. E.; RÜTTEN, S.; MATTOSO, L. H. C.; ZENKE, M.; SECHI, A..
bitstream/item/179094/1/P-17.052-Solution-blow-spinning-fibres....pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Solutions blow spinning; Dendritic cell; Cell motility; Cytoskeleton.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081447
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The cytoskeleton of the electric tissue of Electrophorus electricus, L. Anais da ABC (AABC)
MERMELSTEIN,CLAUDIA DOS SANTOS; COSTA,MANOEL LUIS; MOURA NETO,VIVALDO.
The electric eel Electrophorus electricus is a fresh water teleost showing an electrogenic tissue that produces electric discharges. This electrogenic tissue is distributed in three well-defined electric organs which may be found symmetrically along both sides of the eel. These electric organs develop from muscle and exhibit several biochemical properties and morphological features of the muscle sarcolema. This review examines the contribution of the cytoskeletal meshwork to the maintenance of the polarized organization of the electrocyte, the cell that contains all electric properties of each electric organ. The cytoskeletal filaments display an important role in the establishment and maintenance of the highly specialized membrane model system of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrophorus electricus; Cytoskeleton; Desmin; Actin; Alpha-actinin; Vinculin.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300008
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The in vitro comparative cytopathology of a porcine rotavirus and the simian prototype (SA-11) Arq. Bras. Med. Vet. Zootec.
Lonien,S.C.H.; Bolognini,A.M.; Linhares,R.E.C.; Nozawa,C.M..
A citopatologia in vitro de uma cepa de rotavírus porcino adaptado em cultura de células foi comparada à estirpe-protótipo símia (SA-11). O efeito citopático (ECP) produzido pelos vírus foi semelhante embora a estirpe porcina tivesse apresentado algumas alterações diferentes, como o acentuado estreitamento do citoplasma, com grande perda do volume citoplasmático. O vírus porcino apresentou menor número de plaques de ECP porém com diâmetro maior em relação ao vírus símio, demonstrando maior capacidade de disseminação célula-célula, quase oito vezes mais, a julgar pelo diâmetro dos plaques de ECP. Os elementos do citoesqueleto das células infectadas revelaram uma reorganização semelhante para ambas as estirpes, não sendo possível observar nenhuma diferença,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Rotavirus; Cytopathology; Cell culture; Plaque; Cytoskeleton.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400002
Registros recuperados: 13
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