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Antagonistic interactions between benzo[a]pyrene and C60 in toxicological response of Marine Mussels ArchiMer
Barranger, Audrey; Langan, Laura M; Sharma, Vikram; Rance, Graham A; Aminot, Yann; Weston, Nicola J; Akcha, Farida; Moore, Michael N; Arlt, Volker M; Kholbystov, Andrei N; Readman, James W; Jha, Awadhesh N..
This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR)....
Tipo: Text Palavras-chave: Trojan Horse effect; B[a]P; NC60; Co-exposure; Mussels; DNA damage; Proteomics.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00498/60946/64340.pdf
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Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels ArchiMer
Barranger, Audrey; Langan, Laura M.; Sharma, Vikram; Rance, Graham A.; Aminot, Yann; Weston, Nicola J.; Akcha, Farida; Moore, Michael N.; Arlt, Volker M.; Khlobystov, Andrei N.; Readman, James W.; Jha, Awadhesh N..
This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography–Mass Spectrometry (GC–MS), Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP–MS). Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the interactive effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1...
Tipo: Text Palavras-chave: Trojan horse effect; B[a]P; NC(60); Co-exposure; Mussels; DNA damage; Proteomics.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00506/61780/65779.pdf
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Anthocyanin-rich extract decreases indices of lipid peroxidation and DNA damage in vitamin E-depleted rats IPB - Escola Superior Agrária
Ramirez-Tortosa, Carmen; Cabrita, Luís; Andersen, Øyvind M.; Gardner, P.T.; Morrice, P.C.; Wood, S.G.; Duthie, S.J.; Collins, A.R.; Garry, G.G..
Anthocyanins are secondary plant metabolites responsible for the blue, purple, and red color of many plant tissues. The phenolic structure of anthocyanins conveys marked antioxidant activity in model systems via donation of electrons or hydrogen atoms from hydroxyl moieties to free radicals. Dietary intakes of anthocyanins may exceed 200 mg/day, however, little is known about their antioxidant potency in vivo. Consequently, the aim of this study was to establish whether anthocyanins could act as putative antioxidant micronutrients. Rats were maintained on vitamin E-deficient diets for 12 weeks in order to enhance susceptibility to oxidative damage and then repleted with rations containing a highly purified anthocyanin-rich extract at a concentration of 1...
Tipo: Article Palavras-chave: Anthocyanins; Polyphenols; Lipid peroxides; DNA damage; Antioxidants; Free radicals.
Ano: 2001 URL: http://hdl.handle.net/10198/3368
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Anticancer properties and enhancement of therapeutic potential of cisplatin by leaf extract of Zanthoxylum armatum DC Biol. Res.
Singh,Thangjam Davis; Meitei,Heikrujam Thoihen; Sharma,Adhikarimayum Lakhikumar; Robinson,Asem; Singh,Lisam Shanjukumar; Singh,Thiyam Ramsing.
BACKGROUND: Clinical use of chemotherapeutic drug, cisplatin is limited by its toxicity and drug resistance. Therefore, efforts continue for the discovery of novel combination therapies with cisplatin, to increase efficacy and reduce its toxicity. Here, we screened 16 medicinal plant extracts from Northeast part of India and found that leaf extract of Zanthoxylum armatum DC. (ZALE) induced cytotoxicity as well as an effect on the increasing of the efficiency of chemotherapeutic drugs (cisplatin, mitomycin C and camptothecin). This work shows detail molecular mechanism of anti-cancer activity of ZALE and its potential for combined treatment regimens to enhance the apoptotic response of chemotherapeutic drugs. RESULTS: ZALE induced cytotoxicity, nuclear...
Tipo: Journal article Palavras-chave: Zanthoxylum armatum; Apoptosis; ERK activation; Cisplatin sensitization; DNA damage.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100046
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Antioxidant and free radical scavenging effects in extracts of the medicinal herb Achyrocline satureioides (Lam.) DC. ("marcela") BJMBR
Desmarchelier,C.; Coussio,J.; Ciccia,G..
Achyrocline satureioides (Lam.) DC. (Compositae) is a medicinal herb used in Argentina, Uruguay, Brazil and Paraguay for its choleretic, antispasmodic and hepatoprotective properties. The presence of the flavonoid quercetin and its derivatives, and of different phenolic acids such as caffeic, chlorogenic and isochlorogenic acids in the aerial parts of this plant has led us to study the antioxidant activity of its extracts using different bioassays. The inhibition of luminol-enhanced chemiluminescence by the aqueous and methanolic extracts was used to show that their total reactive antioxidant potential index (TRAP; in µM Trolox equivalents) was 91.0 ± 15.4 and 128.1 ± 20.1 µM, respectively, while the total antioxidant reactivity index (TAR) was calculated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Achyrocline satureioides; Antioxidant activity; Luminol-enhanced chemiluminescence; Thiobarbituric acid-reactive substances; Hydroperoxide-initiated chemiluminescence; Lipid peroxidation; DNA damage; Reactive oxygen species.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900010
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Cobalt oxide nanoparticles aggravate DNA damage and cell death in eggplant via mitochondrial swelling and NO signaling pathway Biol. Res.
Faisal,Mohammad; Saquib,Quaiser; Alatar,Abdulrahman A.; Al-Khedhairy,Abdulaziz A.; Ahmed,Mukhtar; Ansari,Sabiha M.; Alwathnani,Hend A.; Dwivedi,Sourabh; Musarrat,Javed; Praveen,Shelly.
BACKGROUND: Despite manifold benefits of nanoparticles (NPs), less information on the risks of NPs to human health and environment has been studied. Cobalt oxide nanoparticles (Co3O4-NPs) have been reported to cause toxicity in several organisms. In this study, we have investigated the role of Co3O4-NPs in inducing phytotoxicity, cellular DNA damage and apoptosis in eggplant (Solanum melongena L. cv. Violetta lunga 2). To the best of our knowledge, this is the first report on Co3O4-NPs showing phytotoxicity in eggplant. RESULTS: The data revealed that eggplant seeds treated with Co3O4-NPs for 2 h at a concentration of 1.0 mg/ml retarded root length by 81.5 % upon 7 days incubation in a moist chamber. Ultrastructural analysis by transmission electron...
Tipo: Journal article Palavras-chave: Cobalt oxide nanoparticles; Nanotoxicity; DNA damage; Apoptosis; Oxidative stress.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100020
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Cytogenetic and cytomolecular delayed damage induced in human fibroblasts by low X-ray doses JBAG
Ponzinibbio,MV; Crudeli,C; Peral García,P; Seoane,A.
During the last decade several reports have provided evidence on the non-targeted effects of ionizing radiation. In this study, we have analyzed the delayed DNA damage induced by ionizing radiation in MRC-5 Human fibroblasts cultured in vitro. MRC-5 cells were irradiated with 10 and 50 milligrays (mGy) and analyzed immediately after exposure, and at passages 6 and 12 post-radiation by means of comet assay, micronucleus analysis and &#947;-H2AX foci formation. Cytogenetic and cytomolecular damage was analysed at every sample point comparing irradiated and control cells. No significant damage differences were found among samples from all passages of treatments. Our results suggest that even at low doses, ionizing radiation could induce delayed damage....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ionizing radiation; Low radiation dose; DNA damage.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332008000100006
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Daño genético persistente inducido por venenos de topoisomerasa II en fibroblastos humanos normales: generación de inestabilidad cromosómica JBAG
de Campos-Nebel,M; Palmitelli,M; Acevedo,S; González-Cid,M.
Los venenos de topoisomerasa II (Top2) idarubicina (IDA) y etopósido (ETO) son drogas antitumorales que actúan sobre Top2 estabilizando complejos ADN-enzima y generando rupturas de doble cadena (RDC). Estas lesiones en el ADN son peligrosas debido a que llevan a inestabilidad genómica y a promover tumorigénesis. Estas drogas están asociadas al desarrollo de leucemias caracterizadas por translocaciones del gen MLL en los pacientes tratados. Nuestro objetivo fue analizar el daño genético residual inducido por IDA y ETO en fibroblastos humanos normales a diferentes tiempos. Las células se trataron con concentraciones subletales de IDA y ETO durante 2 hs y se evaluaron RDC persistentes en núcleos interfásicos y aberraciones cromosómicas (AC) en metafases a las...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Idarubicina; Etopósido; Daño al ADN; Gen MLL; Células humanas normales; Idarubicin; Etoposide; DNA damage; MLL gene; Normal human cells.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332016000300003
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DNA damage and related modifier genes in Italian Cystic fibrosis patients Biol. Res.
STERPONE,SILVIA; CORNETTA,TOMMASO; ANGIONI,ADRIANO; FISCARELLI,ERSILIA; LUCIDI,VINCENZINA; TESTA,ANTONELLA; COZZI,RENATA.
Cystic Fibrosis (CF) is an autosomal recessive multisystemic disorder showing a highly heterogeneous phenotype, even among siblings carrying identical CFTR mutations. Moreover, oxidative stress is of central importance in the pathogenesis of cystic fibrosis. The present study seeks to value the presence of oxidative damage in CF patients and the possible modifier effect of repair and glutathione-S-transferase genes. We analysed the presence of DNA damage in leukocytes of 63 CF patients at an Italian CF centre and 63 controls, through the alkaline Comet assay to detect DNA strand breaks. Furthermore, controls and 93 CF subjects were genotyped for 5 genes by RFLP-PCR (XRCC1,0GG1,GSTP1) and PCR assay (GSTM1, GSTT1). No difference in Comet assay values was...
Tipo: Journal article Palavras-chave: Comet assay; Cystic fibrosis; DNA damage; Genetic polymorphisms; Modifier genes.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400009
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Environmental concentrations of benz[a]anthracene induce developmental defects and DNA damage and impair photomotor response in Japanese medaka larvae ArchiMer
Le Bihanic, Florane; Sommard, Vivien; De Lansalut, Perrine; Pichon, Anaïk; Grasset, Julie; Berrada, Saadia; Budzinski, Helene; Cousin, Xavier; Morin, Benedicte; Cachot, Jerome.
Benz[a]anthracene (BaA) is a ubiquitous polycyclic aromatic hydrocarbon found in numerous aquatic ecosystems. However, ecotoxicological data in aquatic organisms are scarce. To remedy this lack of data, Japanese medaka (Oryzias latipes) embryos were exposed to BaA and toxic effects were investigated at multiple toxicological endpoints. Japanese medaka embryos were incubated onto BaA-spiked artificial sediment for 9 days at low or moderate environmental concentrations ranging from 0.9 to 12 µg g−1 dw. BaA-exposed embryos exhibited significant tachycardia. BaA exposure was also shown to increase CYP1A activity in the hepato-biliary tissue as well as craniofacial deformities and DNA damage in pro-larvae. The photomotor response of BaA-exposed larvae was...
Tipo: Text Palavras-chave: Benz[a]anthracene; Spiked sediment; Teratogenicity; DNA damage; Swimming behavior; In vivo EROD activity.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00244/35513/34729.pdf
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GENOMIC INTEGRITY EVALUATION IN SPERM OF CHOROMYTILUS CHORUS (MOLINA, 1782) BY COMET ASSAY Gayana
Lafarga-De la Cruz,Fabiola; Valenzuela-Bustamante,Myriam; Dupré,Enrique; del Río-Portilla,Miguel A; Gallardo-Escárate,Cristián.
The comet assays or single cell gel electrophoresis is a simple, rapid, relatively inexpensive and very sensitive method that has been recently widely used to evaluate genomic integrity (or DNA damage) in aquatic animals exposed to diverse physical and chemical agents. However, the accuracy to evaluate comets assay has been the major obstacle in order to determine specific damage undergone cellular stress. The aim of this study was to evaluate DNA fragmentation in sperm cells based in fluorescence image analysis. The species used was the giant mussel Choromytilus chorus. The in vitro experiments were carried out through oxidative stress with five hydrogen peroxide concentrations (0, 50, 100, 500 and 1000 mM). To evaluate DNA damage two groups of parameters...
Tipo: Journal article Palavras-chave: Single cell gel electrophoresis; DNA damage; Image analysis.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000100006
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Integridade de DNA e morfologia espermática de ratos submetidos a campos eletromagnéticos de baixa frequência durante diferentes períodos do desenvolvimento - doi: 10.4025/actascibiolsci.v33i3.5417 Biological Sciences
Tenório, Bruno Mendes; Universidade Federal Rural de Pernambuco; Jimenez, George Chaves; UFRPE; Guerra, Maria Madalena Pessoa; UFRPE; Nogueira, Romildo de Albuquerque; UFRPE; Silva Junior, Valdemiro Amaro da; UFRPE.
A exposição da sociedade a Campos Eletromagnéticos (CEM) vem aumentando vertiginosamente em virtude da ampla expansão tecnológica observada nos últimos anos. Tanto a geração, como a distribuição e a utilização de energia elétrica podem gerar Campos Eletromagnéticos de baixa freqüência (50 e 60 Hz). Pesquisas vêm demonstrando que a exposição a estes CEM podem proporcionar alterações fisiológicas significativas, apesar disto, ainda não estão totalmente esclarecidos a extensão destes efeitos, nem os mecanismos de ação que envolve a interação dos CEM com os organismos biológicos. O presente trabalho teve como principal objetivo verificar os efeitos dos CEM (60 Hz e 1 mT) sobre a integridade de DNA e morfologia espermática de ratos sexualmente maduros, que...
Tipo: Estudos Morfológicos; Itegridade de DNA Palavras-chave: 2.06.00.00-3; 2.07.00.00-8 espermatozoide; Lesões de DNA; Patologias espermáticas Morfologia; Fisiologia electromagnetic wave; Non-ionizing radiation; Spermatozoa; DNA damage; Sperm pathologies.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5417
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Investigating the relationship between embryotoxic and genotoxic effects of benzo[alpha]pyrene, 17 alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos ArchiMer
Wessel, Nathalie; Rousseau, Sabrina; Caisey, Xavier; Quiniou, Francoise; Akcha, Farida.
Genotoxicity biomarkers are widely measured in ecotoxicology as molecular toxic endpoints of major environmental pollutants. However, the long-term consequences of such damage still have to be elucidated. Some authors have suggested that the accumulation of unrepaired DNA lesions could explain the embryotoxicity of certain chemical pollutants. As embryotoxicity exerts a direct impact on the recruitment rate, genotoxicity could be closely related to disturbances of ecological concern and produce a possible impact upon population dynamics. The aim of the present work was to study the genotoxicity and the embryotoxicity of three relevant pollutants for oyster embryos: the polycyclic aromatic hydrocarbon, benzo[a]pyrene (BaP), the synthetic estrogenic...
Tipo: Text Palavras-chave: 17 alpha ethinylestradiol; BaP; Endosulfan; DNA damage; Embryotoxicity; Pacific oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4058.pdf
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Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas ArchiMer
Barranger, Audrey; Heude-berthelin, Clothilde; Rouxel, Julien; Adeline, Beatrice; Benabdelmouna, Abdellah; Burgeot, Thierry; Akcha, Farida.
Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography...
Tipo: Text Palavras-chave: Crassostrea gigas; Oxidative stress; DNA damage; Germ cell; Pesticides.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00295/40655/39753.pdf
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Protective effects of spray-dried açaí (Euterpe oleracea Mart) fruit pulp against initiation step of colon carcinogenesis. Repositório Alice
ROMUALDO, G. R.; FRAGOSO, M. F.; BORGUINI, R. G.; SANTIAGO, M. C. P. de A.; FERNANDES, A. A. H.; BARBISAN, L. F..
bitstream/item/136871/1/Food-Res-Internat-15-final-1.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Açaí (Euterpe oleracea Mart); Colon carcinogenesis; Male Swiss mice; DNA damage; Azoxymethane.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1033481
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Responses of the European flounder (Platichthys flesus) to a mixture of PAHs and PCBs in experimental conditions ArchiMer
Dupuy, Celie; Galland, Claire; Devaux, Alain; Bony, Sylvie; Loizeau, Veronique; Danion, Morgane; Pichereau, Vianney; Fournier, Michel; Laroche, Jean.
A multibiomarker approach was developed to evaluate the juvenile European flounder responses to a complex mixture of 9 polycyclic aromatic hydrocarbons (PAHs) and 12 polychlorinated biphenyls (PCBs). Exposure was performed through contaminated food pellets displaying: (1) PAH and PCB levels similar to those detected in the heavily polluted Seine estuary, respectively in sediments and in flatfish and (2) ten times these concentrations. Several biomarkers of the immune system (e.g., lysozyme concentration and gene expression of complement component C3 and TNF-receptor), DNA damage (e.g., Comet assay), energetic metabolism (e.g., activity of cytochrome C oxidase), detoxification process (e.g., cytochrome P450 1A1 expression level: CYP1A1; betaine homocysteine...
Tipo: Text Palavras-chave: Mixture of contaminants; Fish; Biomarker; Immunotoxicity; Detoxification process; DNA damage; PAHs; PCBs.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29914/28374.pdf
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Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line Biol. Res.
Votto,Ana P. S; Domingues,Beatriz S; de Souza,Michele M; da Silva Júnior,Flavio M. R; Caldas,Sergiane S; Filgueira,Daza M. V. B; Clementin,Rosilene M; Primel,Ednei G; Vallochi,Adriana L; Furlong,Eliana B; Trindade,Gilma S.
Onion (Allium cepa) is being studied as a potential anticancer agent, but little is known regarding its effect in multidrug resistance (MDR) cells. In this work, the cytotoxicity of crude onion extract (OE) and fractioned extract (aqueous, methanolic and ethyl acetate), as well as some onion compounds (quercetin and propyl disulfide) were evaluated in Lucena MDR human erythroleukemic and its K562 parental cell line. The capacity of OE to induce apoptosis and/or necrosis in these cells, the possible participation of oxidative stress and DNA damage were also assessed. Similar sensitivities were obtained for both tumoral cells, however only OE caused significant effects in the cells. In K562 cells, a significant increase of apoptosis was verified while the...
Tipo: Journal article Palavras-chave: Antioxidant; Apoptosis and/or necrosis; DNA damage; MDR phenotype; Onion (Allium cepa); Tumoral cell.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400007
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