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Registros recuperados: 23
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An isolate of sweet potato chlorotic stunt virus from Brazil with a distinct genome organization. Repositório Alice
ROSSATO, M.; SOUZA, C. A.; MELO, F. L.; ORILIO, A. F.; RESENDE, R. O.; RIBEIRO, S. G.; NAITO, F. Y. B.; RIBEIRO, G. P.; ANDRADE, G. P.; PEREIRA?CARVALHO, R. C..
bitstream/item/202669/1/Rossato2019-Article-AnIsolateOfSweetPotatoChloroti.pdf
Tipo: Nota Técnica/Nota Científica (ALICE) Palavras-chave: Genetic research; Genomes; DNA sequencing; Phylogeny; Genomics.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112895
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Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer Biol. Res.
JIAN,HU; LI,SUN; LU-MING,WANG; SU-JUN,JIANG.
Several studies revealed a similar down-regulation of telomeric repeat binding factor 1 (TRF1) in tumors. We have previously reported the TRFl expression levels were down-regulation in non-small cell lung cancer (NSCLC). The regulation of TRFl localization is proposed to be important for the function and expression. The nuclear localization signal (NLS) and nuclear export signal (NES) are often important clues to localization of protein. The objective of the present study was to investigate the NLS and NES of TRFl in NSCLC patients. Thirty (30) patients with NSCLCs had undergone radical operations in The First Affiliated Hospital, College of Medicine, Zhejiang University. DNA sequences of NLSs and NESs were amplified by PCR. The PCR products were analyzed...
Tipo: Journal article Palavras-chave: Non-small cell lung cancer; Telomeric repeat binding factor 1; Nuclear of localization signal; Nuclear of export signal; DNA sequencing.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200010
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Applications of genomics to rice breeding International Rice Research Institute
Mackill, D. J..
p.9-15
Palavras-chave: Breeding; Genome analysis; Gene mapping; DNA sequencing; Genetic markers; Genetic markers; Genetic transformation; Gene expression.
Ano: 2003 URL: http://hdl.handle.net/123456789/1307
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Association of MYF5 gene allelic variants with production traits in pigs Genet. Mol. Biol.
Carmo,Fausto Moreira da Silva; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Guimarães,Marta Fonseca Martins; Silva,Marcos Vinícius Gualberto Barbosa da; Schierholt,Alex Sandro; Silva,Kleibe de Moraes e; Gomide,Lúcio Alberto de Miranda.
We studied the phenotypic effects of polymorphisms at the MYF5 gene in a divergent F2 swine population and found that one polymorphism was due to an insertion and another to a deletion. The genotypes of 359 F2 animals were obtained and the Normal/Normal (NN) and Normal/Insertion (NI) genotypes analyzed to determine associations with phenotypic data for performance, carcass and meat quality traits. Significant differences were observed (p < 0.05) between NN and NI animals for drip (NN = 3.14 ± 1.56; NI = 3.69 ± 2.78%), cooking (NN = 32.26 ± 2.41; NI = 33.21 ± 2.31%) and total loss (NN = 34.16 ± 2.63 and NI = 34.97 ± 2.08%). The Deletion marker was not statistically tested. The results indicate that the allelic variant Insertion is associated with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myogenesis; DNA sequencing; Pig production; PCR.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300004
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Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs Arq. Bras. Med. Vet. Zootec.
Figueiredo,F.C.; Lopes,P.S.; Pinto,A.P.G.; Paiva,D.A.F.; Mendonça,P.T.; Guimarães,S.E.F..
The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Candidate gene; Molecular marker; DNA polymorphism; DNA sequencing.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200020
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Calmodulin gene as good voucher as MALDI-TOF ICMS to identify Portuguese isolates of Aspergillus section Flavi IPB - Escola Superior Agrária
Rodrigues, Paula; Santos, Cledir; Venâncio, Armando; Lima, Nelson.
Aspergillus is a large genus, with a complex and ever evolving taxonomy. Section Flavi is one of the most significant Sections in the Genus. Taxonomy and species identification is subject of great interest for scientists aiming to clarify the species concept and limits within the section. Furthermore, this Section comprises both toxigenic and non-toxigenic species/strains, with great interest to biotechnology and food industry. Various genes, namely the rRNA (internal transcribed spacer (ITS) and partial LSU-rRNA regions), calmodulin and ~-tubulin genes, have been widely reported as good markers for Aspergillus species identification, because they are rapid and cost-effective.
Tipo: ConferenceObject Palavras-chave: Fungal identification; DNA sequencing; Mass spectrometry.
Ano: 2010 URL: http://hdl.handle.net/10198/4276
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CYP21 gene mutations in Brazilian patients with 21-hydroxylase deficiency from the Amazon region Genet. Mol. Biol.
Carvalho,Tarcísio André Amorim de; Souza,Izabel Cristina Neves de; Yoshioka,France Keiko Nascimento; Caldato,Milena Coelho Fernandes; Torres,Nilza Nei; Garcia,Lena Stilianidi; Guerreiro,João Farias.
Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (P450c21, CYP21) accounts for about 95% of all CAH cases. The incidence of CYP21 gene mutations has been extensively studied in the last years, but in Brazil it has been investigated only in Southeast Brazilian patients. This study is the first report on the distribution of CYP21 mutations in patients from the Amazon region. Direct sequencing of the CYP21 gene identified at least one mutation in 96% of the studied chromosomes. The most common mutations found were IVS2-13A/C > G (36%), Q318X (12%), V281L (12%), 1760_1761insT (9%), Cluster E6 (7%), and P30L (7%). The worldwide most common mutations were identified among patients from the Amazon region at frequencies that may be expected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon region; 21-hydroxylase deficiency; DNA sequencing.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400004
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Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples BJID
Macedo,Carla Isabel; Carnieli Jr,Pedro; Brandão,Paulo Eduardo; Rosa,Elizabeth S. Travassos da; Oliveira,Rafael de Novaes; Castilho,Juliana Galera; Medeiros,Rita; Machado,Rosangela Rocha; Oliveira,Rosely Cerqueira de; Carrieri,Maria Luiza; Kotait,Ivanete.
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human rabies; Neck-skin; Diagnosis; RT-PCR; DNA sequencing.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008
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Epidemiological and functional implications of molecular variants of human papillomavirus BJMBR
Sichero,L.; Villa,L.L..
Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; DNA sequencing; Oncogenic potential; Cervical cancer; Immunity; Epidemiology.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600002
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Low frequency of p53 mutations in cervical carcinomas among Brazilian women BJMBR
Pinheiro,N.A.; Villa,L.L..
Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Single-stranded conformation polymorphism; SSCP; Suppressor gene; HPV; PCR; DNA sequencing.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005
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Low mtDNA diversity in a highly differentiated population of spinner dolphins (Stenella longirostris) from the Fernando de Noronha Archipelago, Brazil. Repositório Alice
FARIA, D. M.; SILVA JÚNIOR, J. M. da; COSTA, L. P.; PAIVA, S. R.; MARINO, C. L.; ROLLO JÚNIOR, M. M.; BAKER, C. S.; FARRO, A. P. C..
bitstream/item/213180/1/journal.pone.0230660.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Stenella longirostris; DNA sequencing; Archipelagoes; Brazil; Dolphins; Mitochondrial DNA; Haplotypes.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122499
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Medium-sized deletion in the BRCA1 gene: limitations of Sanger sequencing and MLPA analyses Genet. Mol. Biol.
Herman,Sandra; Varga,Dominic; Deissler,Heidrun L.; Kreienberg,Rolf; Deissler,Helmut.
We describe a family with a history of breast and ovarian cancer in which MLPA analysis of the BRCA1 gene pointed to a deletion including a part of exon 11. Further characterization confirmed a loss of 374 bp in a region completely covered by conventional sequencing which had not revealed the deletion. Because this alteration was only detected serendipitously with an MLPA probe, we calculated the probabilities of detecting medium-sized deletions in large exons by methods including initial PCR amplification. This showed that a considerable fraction of medium-sized deletions are undetectable by currently used standard methods of mutation analyses. We conclude that long, widely overlapping amplicons should be used to minimize the risk of missing medium-sized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mutation analysis; DNA sequencing; PCR; Hereditary breast cancer; Model calculations.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100007
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Microbiomes of Velloziaceae from phosphorus-impoverished soils of the campos rupestres, a biodiversity hotspot. Repositório Alice
CAMARGO, A. P.; SOUZA, R. S. C. de; COSTA, P. de B.; GERHARDT, I. R.; DANTE, R. A.; TEODORO, G. S.; ABRAHÃO, A.; LAMBERS, H.; CARAZZOLLE, M. F.; HUNTEMANN, M.; CLUM, A.; FOSTER, B.; FOSTER, B.; ROUX, S.; PALANIAPPAN, K.; VARGHESE, N.; MUKHERJEE, S.; REDDY, T. B. K.; DAUM, C.; COPELAND, A.; CHENM U, M. A.; IVANOVA, N. N.; KYRPIDES, N. C.; PENNACCHIO, C.; ELOE-FADROSH, E. A.; ARRUDA, P.; OLIVEIRA, R. S..
The rocky, seasonally-dry and nutrient-impoverished soils of the Brazilian campos rupestres impose severe growth-limiting conditions on plants. Species of a dominant plant family, Velloziaceae, are highly specialized to low-nutrient conditions and seasonal water availability of this environment, where phosphorus (P) is the key limiting nutrient. Despite plant-microbe associations playing critical roles in stressful ecosystems, the contribution of these interactions in the campos rupestres remains poorly studied. Here we present the first microbiome data of Velloziaceae spp. thriving in contrasting substrates of campos rupestres. We assessed the microbiomes of Vellozia epidendroides, which occupies shallow patches of soil, and Barbacenia macrantha, growing...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Microbioma de plantas; Campos rupestres brasileiros; Sequenciamento genético; Condições extremas; Solos; Stressful environments; DNA sequencing; Soils; Microrganismo; Velloziaceae; Microorganisms; Microbiome.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1111238
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Molecular characterization of 'Candidatus Liberibacter' species/strains causing huanglongbing disease of citrus in Kenya Electron. J. Biotechnol.
Magomere,Titus O; Obukosia,Silas D; Mutitu,Eunice; Ngichabe,Christopher; Olubayo,Florence; Shibairo,Solomon.
This study was undertaken to characterize the alpha subgroup of the proteobacteria causing the huanglongbing (HLB) disease of citrus from three different ecological zones of Kenya namely the Lower highlands (LH2, LH3, 1800-1900 m above sea level); Upper midlands (UM3, UM4, 1390-1475m), Lower midlands (LM5, LM4, LM3 of 1290-1340-1390m), by isolation and sequencing DNA encoding the L10 and L12 ribosomal proteins and the intergenic region. A 7I6-basepair DNA fragment was amplified and sequenced and consisted of 536 basepairs of DNA encoding the L10 protein, 44 basepairs of DNA intergenic region and 136 basepairs of DNA that partially encodes the L12 protein. Sequences of rpL10/L12 protein genes from Kenyan strains were 98% and 81% similar to the South African...
Tipo: Journal article Palavras-chave: Citrus greening disease; DNA sequencing; Intergenic regions; Phylogeny; Ribosomal protein genes.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200005
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Molecular characterization of the polyphenol oxidase gene in lulo (Solanum quitoense Lam.) var. Castilla Braz. J. Plant Physiol.
Arias,Duverney Gaviria; Doria,Clara María Mejía; Ramos,Lucero Rengifo; Morocho,Heydy Catalina Navia.
Lulo (Solanum quitoense Lam.) is an exotic fruit from the Andes Mountains with a high export potential. However, the browning that is produced during harvest and the postharvest processes alters the organoleptic and nutritional properties of this fruit, which has made its management as a fresh fruit difficult. The browning processes are regulated by the enzyme family of the polyphenol oxidases (PPOs) located in the thylakoids of the chloroplast. When there is damage at the tissue level, the phenolic compounds found in the vacuoles enter into contact with the polyphenol oxidase. This enzyme produces polymerization among the phenolic compounds, as well as between them and the proteins and cell walls. This study analyzed the polyphenol oxidase in lulo var....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic browning; DNA sequencing; Solanaceae; UTRA domain.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000400005
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Molecular investigation of a fungemia outbreak due to Candida parapsilosis in an intensive care unit BJID
Dizbay,Murat; Kalkanci,Ayse; Sezer,Busra Ergut; Aktas,Firdevs; Aydogan,Sibel; Fidan,Isil; Kustimur,Semra; Sugita,Takashi.
We investigated a nosocomial cluster of four Candida parapsilosis fungemia episodes that occurred in a neurological intensive care unit over a two-week period. The four infected patients had received parenteral nutrition through central lines, and all four had catheter-related candidemia. All of the isolates were susceptible to all of the antifungals tested, including amphotericin B, fluconazole, voriconazole, and caspofungin. They had strictly related fingerprints, based on randomly amplified polymorphic DNA analysis. Additional DNA sequencing data revealed that they were same strain. Although no isolate of Candida parapsilosis was recovered from other clinical, surveillance, or environmental samples, nosocomial spread of this yeast ceased, following the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidemia; Candida parapsilosis; Nosocomial outbreak; Intensive care unit; RAPD; DNA sequencing.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500010
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Molecular systematics of the Phyllachorales (ascomycota, fungi) based on 18S ribosomal DNA sequences BABT
Wanderlei-Silva,Denise; Ramalho Neto,Eduardo; Hanlin,Richard.
In order to evaluate the monophyly of the Phyllachorales from a molecular standpoint and elucidate its phylogenetic relationships with other orders, a segment of the 18S rRNA gene from several representatives of the Phyllachorales, including species of Glomerella, Phyllachora, Coccodiella (=Coccostroma), Sphaerodothis, Ophiodothella, as well as Magnaporthe was sequenced. Maximum Parsimony analysis revealed that the Phyllachorales was a polyphyletic assemblage of taxa. None of the other members of the Phyllachorales, which produced either a clypeus or stroma, clustered with Glomerella. Of the taxa examined, was Coccodiella the closest relative of Phyllachora. Magnaporthe was closely related to the Diaporthales. Our 18S rDNA data highly supported Glomerella...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA sequencing; 18S ribosomal DNA; PCR; Phyllachorales.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300002
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Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle. Repositório Alice
GRISOLIA, A. B.; D´ANGELO, G. T.; PORTO NETO, L. R.; SIQUEIRA, F.; GARCIA, J. F..
The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue and Blond?Aquitaine, and others) show polymorphisms in this gene; these polymorphisms are directly related to the double muscling phenotype. We looked for polymorphisms in the Nellore cattle myostatin gene and compared them with those known for taurine breeds. Seven regions, covering the three exons of this gene, were amplified by polymerase chain reaction and sequenced, including the untranslated region. DNA from 30...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bovino de corte; Melhoramento genético animal; Nelore; DNA; Carne; Animal breeding; DNA sequencing; Double muscling; Nucleotides.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/572633
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Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae). Repositório Alice
ALMEIDA, R. P. de; STOUTHAMERB, R..
Wolbachia (Hertig) endosymbionts are extensively studied in a wide range of organisms and are known to be transmitted through the egg cytoplasm to the offsping. Wolbachia may cause several types of reproductive modifications in arthropods. In Trichogramma species, parthenogenesis-inducing Wolbachia bacteria allow females wasps to produce daughters from unfertilized eggs and these bacteria are present in at least 9% of all Trichogramma species. Phylogenetic studies have led to the subdivision of the Wolbachia clade in five supergroups (A, B, C, D and E) and Wolbachia from Trichogramma belong to supergroup B. Here, using the wsp gene, four groups of Wolbachia that infect Trichogramma species were distinguished and the addition of a new group "Ato" was...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Filogenia; DNA sequencing; Phylogenetic relationships; Symbionts; Trichogramma; Wolbachia; Alpha-proteobacteria.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1083383
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Reassessment of the genome size in elaeis guineensis and elaeis oleifera, and its interspecific hybrid. Repositório Alice
CAMILLO, J.; LEAO, A. P.; ALVES, A. A.; FORMIGHIERI, E. F.; AZEVEDO, A. L. S.; NUNES, J. D.; CAPDEVILLE, G. de; MATTOS, J. K. de. A.; SOUZA JUNIOR, M. T..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: African oil palm; American oil palm; DNA sequencing; FCM-flow cytometry; GC content.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010471
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