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Registros recuperados: 9
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A two-plasmid strategy for engineering a dengue virus type 3 infectious clone from primary Brazilian isolate Anais da ABC (AABC)
SANTOS,JEFFERSON J.S.; CORDEIRO,MARLI T.; BERTANI,GIOVANI R.; MARQUES,ERNESTO T.A.; GIL,LAURA H.V.G..
Dengue infections represent one of the most prevalent arthropod-borne diseases worldwide, causing a wide spectrum of clinical outcomes. Engineered infectious clone is an important tool to study Dengue virus (DENV) biology. Functional full-length cDNA clones have been constructed for many positive-strand RNA viruses and have provided valuable tools for studying the molecular mechanisms involved in viral genome replication, virion assembly, virus pathogenesis and vaccine development. We report herein the successful development of an infectious clone from a primary Brazilian isolate of dengue virus 3 (DENV3) of the genotype III. Using a two-plasmid strategy, DENV3 genome was divided in two parts and cloned separately into a yeast-bacteria shuttle vector. All...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reverse genetics; Dengue virus; Molecular cloning; Infectious clone.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401749
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Accuracy of immunoglobulin M and immunoglobulin A of saliva in early diagnosis of dengue: Systematic Review and Meta-analysis Anais da ABC (AABC)
COLONETTI,TAMY; ROCHA,BELISE V.E.; GRANDE,ANTÔNIO J.; ALEXANDRE,MARIA C.M.; DONDOSSOLA,EDUARDO R.; MADEIRA,KRISTIAN; ROSA,MARIA I..
ABSTRACT The objective of the study was to conduct a systematic review to synthesize the current evidence on the accuracy of IgM and IgA to early diagnosis the dengue virus. The review protocol was registered at PROSPERO (CRD 42015024808). We searched for studies in the following electronic database from 1990 to January 2018. The search identified 3507 studies. Five studies were included for quantitative analysis. Three studies included evaluations of salivary IgM provided a sensitivity of 86% and specificity of 93%. Two studies included evaluating of IgA salivary showed a combined sensitivity of 69% and a combined specificity of 98%. Despite the results found and the low methodological quality of the studies included in the meta-analysis it is still soon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunoglobulin M; Immunoglobulin A; Antibodies; Dengue virus; Diagnosis..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603147
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Dengue virus tetra-epitope peptide expressed in lettuce chloroplasts for potential use in dengue diagnosis. Repositório Alice
MALDANER, F. R.; ARAGAO, F. J. L.; SANTOS, F. B. dos; FRANCO, O. L.; LIMA, M. da R. Q.; RESENDE, R. de O.; VASQUES, R. M.; NAGATA, T..
bitstream/item/179847/1/Maldaner2013-Article-DengueVirusTetra-epitopePeptid.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tetra-epitope antigen; Transplastomic plant; Dengue diagnostic; Dengue virus.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/964199
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Dengue virus-induced regulation of the host cell translational machinery BJMBR
Villas-Bôas,C.S.A.; Conceição,T.M.; Ramírez,J.; Santoro,A.B.M.; Da Poian,A.T.; Montero-Lomelí,M..
Dengue virus (DV)-induced changes in the host cell protein synthesis machinery are not well understood. We investigated the transcriptional changes related to initiation of protein synthesis. The human hepatoma cell line, HepG2, was infected with DV serotype 2 for 1 h at a multiplicity of infection of one. RNA was extracted after 6, 24 and 48 h. Microarray results showed that 36.5% of the translation factors related to initiation of protein synthesis had significant differential expression (Z-score ≥ ±2.0). Confirmation was obtained by quantitative real-time reverse transcription-PCR. Of the genes involved in the activation of mRNA for cap-dependent translation (eIF4 factors), eIF4A, eIF4G1 and eIF4B were up-regulated while the negative regulator of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue virus; Flavivirus; Protein synthesis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100004
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Differential replicative fitness of the four dengue virus serotypes circulating in Colombia in human liver Huh7 cells BJID
Gutiérrez-Barbosa,Hernando; Castañeda,Nadia Y.; Castellanos,Jaime E..
ABSTRACT Dengue has been a significant public health problem in Colombia since the simultaneous circulation of the four dengue virus serotypes. The replicative fitness of dengue is a biological feature important for virus evolution and contributes to elucidating the behavior of virus populations and viral pathogenesis. However, it has not yet been studied in Colombian isolates. This study aimed to compare the replicative fitness of the four dengue virus serotypes and understand the association between the serotypes, their in vitro infection ability, and their replication in target cells. We used three isolates of each DENV serotype to infect Huh-7 cells at an MOI of 0.5. The percentage of infected cells was evaluated by flow cytometry, cell viability was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue virus; Dengue serotypes; Replicative fitness; Dengue strains.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100013
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Epitope of dengue virus E protein detect human antibodies associated with mild disease: a potential peptide for vaccine development BJID
Sánchez-Burgos,Gilma; Herrera-Nájera,Carla; Cedillo-Rivera,Roberto; Dumonteil,Eric.
ABSTRACT The antigenic potential of seven immunogenic peptides of the dengue virus was evaluated in the sera of patients with dengue confirmed by IgM/IgG serology. Antibodies IgM and IgG against dengue virus peptides were analyzed by ELISA in 31 dengue sero-positive and 20 sero-negative patients. The P5 peptide showed significant IgG immunoreactivity mostly in the sera of patients with dengue without warning signs in comparison with patients with dengue with warning signs, correlating with mild disease. This finding suggests that the low antibody response against P5 epitope could be a risk factor for higher susceptibility to dengue virus infection with warning signs, and that P5 could be a potential antigen for vaccine development.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dengue virus; Epitopes; Peptides; Antibodies.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100085
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Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001 BJMBR
Pires Neto,R.J.; Lima,D.M.; de Paula,S.O.; Lima,C.M.; Rocco,I.M.; Fonseca,B.A.L..
Dengue is a mosquito-borne viral infection that in recent decades has become a major international public health concern. Epidemic dengue fever reemerged in Brazil in 1981. Since 1990 more than one dengue virus serotype has been circulating in this tropical country and increasing rates of dengue hemorrhagic fever and dengue shock syndrome have been detected every year. Some evidence supports the association between the introduction of a new serotype and/or genotype in a region and the appearance of dengue hemorrhagic fever. In order to study the evolutionary relationships and possible detection of the introduction of new dengue virus genotypes in Brazil in the last years, we analyzed partial nucleotide sequences of 52 Brazilian samples of both dengue type...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue virus; Nucleotide sequence; E/NS1 region; Molecular epidemiology; Dengue fever; Dengue hemorrhagic fever.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600005
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Neurological complications and death in children with dengue virus infection: report of two cases J. Venom. Anim. Toxins incl. Trop. Dis.
Osnaya-Romero,Neydi; Perez-Guille,Maria-Gabriela; Andrade-García,Sandra; Gonzalez-Vargas,Erika; Borgaro-Payro,Rebeca; Villagomez-Martinez,Sandra; Ortega-Maldonado,José de Jesús; Arredondo-García,Jose Luis.
Abstract Background Dengue virus infection can have different complications; the best known is hemorrhagic dengue fever. However, other effects such as neurological disorders may endanger the lives of patients. Dengue neurological manifestations can be confused with encephalitis symptoms and can lead to cerebral edema and death. Therefore, we consider important in the endemic areas to take into account the diagnosis of dengue encephalitis in patients with neurological disorders, and to request the determination of serology in cerebrospinal fluid for the NS1 antigen test. Case presentation We present the cases of two patients from the state of Morelos, Mexico, with 17 and 14 years of age. Both cases presented a rapid evolution characterized by fever,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Dengue; Dengue virus; Neurological disorders; Death; Children.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100505
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Transmission of dengue virus from deceased donors to solid organ transplant recipients: case report and literature review BJID
Rosso,Fernando; Pineda,Juan C.; Sanz,Ana M.; Cedano,Jorge A.; Caicedo,Luis A..
ABSTRACT Dengue fever is a vector-transmitted viral infection. Non-vectorial forms of transmission can occur through organ transplantation. We reviewed medical records of donors and recipients with suspected dengue in the first post-transplant week. We used serologic and molecular analysis to confirm the infection. Herein, we describe four cases of dengue virus transmission through solid organ transplantation. The recipients had positive serology and RT-PCR. Infection in donors was detected through serology. All cases presented with fever within the first week after transplantation. There were no fatal cases. After these cases, we implemented dengue screening with NS1 antigen detection in donors during dengue outbreaks, and no new cases were detected. In...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Dengue virus; Transmission; Solid organ transplantation; Screening.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100063
Registros recuperados: 9
Primeira ... 1 ... Última
 

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