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Registros recuperados: 6
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A two-plasmid strategy for engineering a dengue virus type 3 infectious clone from primary Brazilian isolate Anais da ABC (AABC)
SANTOS,JEFFERSON J.S.; CORDEIRO,MARLI T.; BERTANI,GIOVANI R.; MARQUES,ERNESTO T.A.; GIL,LAURA H.V.G..
Dengue infections represent one of the most prevalent arthropod-borne diseases worldwide, causing a wide spectrum of clinical outcomes. Engineered infectious clone is an important tool to study Dengue virus (DENV) biology. Functional full-length cDNA clones have been constructed for many positive-strand RNA viruses and have provided valuable tools for studying the molecular mechanisms involved in viral genome replication, virion assembly, virus pathogenesis and vaccine development. We report herein the successful development of an infectious clone from a primary Brazilian isolate of dengue virus 3 (DENV3) of the genotype III. Using a two-plasmid strategy, DENV3 genome was divided in two parts and cloned separately into a yeast-bacteria shuttle vector. All...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reverse genetics; Dengue virus; Molecular cloning; Infectious clone.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401749
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Dengue virus tetra-epitope peptide expressed in lettuce chloroplasts for potential use in dengue diagnosis. Repositório Alice
MALDANER, F. R.; ARAGAO, F. J. L.; SANTOS, F. B. dos; FRANCO, O. L.; LIMA, M. da R. Q.; RESENDE, R. de O.; VASQUES, R. M.; NAGATA, T..
bitstream/item/179847/1/Maldaner2013-Article-DengueVirusTetra-epitopePeptid.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tetra-epitope antigen; Transplastomic plant; Dengue diagnostic; Dengue virus.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/964199
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Dengue virus-induced regulation of the host cell translational machinery BJMBR
Villas-Bôas,C.S.A.; Conceição,T.M.; Ramírez,J.; Santoro,A.B.M.; Da Poian,A.T.; Montero-Lomelí,M..
Dengue virus (DV)-induced changes in the host cell protein synthesis machinery are not well understood. We investigated the transcriptional changes related to initiation of protein synthesis. The human hepatoma cell line, HepG2, was infected with DV serotype 2 for 1 h at a multiplicity of infection of one. RNA was extracted after 6, 24 and 48 h. Microarray results showed that 36.5% of the translation factors related to initiation of protein synthesis had significant differential expression (Z-score ≥ ±2.0). Confirmation was obtained by quantitative real-time reverse transcription-PCR. Of the genes involved in the activation of mRNA for cap-dependent translation (eIF4 factors), eIF4A, eIF4G1 and eIF4B were up-regulated while the negative regulator of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue virus; Flavivirus; Protein synthesis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100004
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Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001 BJMBR
Pires Neto,R.J.; Lima,D.M.; de Paula,S.O.; Lima,C.M.; Rocco,I.M.; Fonseca,B.A.L..
Dengue is a mosquito-borne viral infection that in recent decades has become a major international public health concern. Epidemic dengue fever reemerged in Brazil in 1981. Since 1990 more than one dengue virus serotype has been circulating in this tropical country and increasing rates of dengue hemorrhagic fever and dengue shock syndrome have been detected every year. Some evidence supports the association between the introduction of a new serotype and/or genotype in a region and the appearance of dengue hemorrhagic fever. In order to study the evolutionary relationships and possible detection of the introduction of new dengue virus genotypes in Brazil in the last years, we analyzed partial nucleotide sequences of 52 Brazilian samples of both dengue type...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue virus; Nucleotide sequence; E/NS1 region; Molecular epidemiology; Dengue fever; Dengue hemorrhagic fever.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600005
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Neurological complications and death in children with dengue virus infection: report of two cases J. Venom. Anim. Toxins incl. Trop. Dis.
Osnaya-Romero,Neydi; Perez-Guille,Maria-Gabriela; Andrade-García,Sandra; Gonzalez-Vargas,Erika; Borgaro-Payro,Rebeca; Villagomez-Martinez,Sandra; Ortega-Maldonado,José de Jesús; Arredondo-García,Jose Luis.
Abstract Background Dengue virus infection can have different complications; the best known is hemorrhagic dengue fever. However, other effects such as neurological disorders may endanger the lives of patients. Dengue neurological manifestations can be confused with encephalitis symptoms and can lead to cerebral edema and death. Therefore, we consider important in the endemic areas to take into account the diagnosis of dengue encephalitis in patients with neurological disorders, and to request the determination of serology in cerebrospinal fluid for the NS1 antigen test. Case presentation We present the cases of two patients from the state of Morelos, Mexico, with 17 and 14 years of age. Both cases presented a rapid evolution characterized by fever,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Dengue; Dengue virus; Neurological disorders; Death; Children.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100505
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Transmission of dengue virus from deceased donors to solid organ transplant recipients: case report and literature review BJID
Rosso,Fernando; Pineda,Juan C.; Sanz,Ana M.; Cedano,Jorge A.; Caicedo,Luis A..
ABSTRACT Dengue fever is a vector-transmitted viral infection. Non-vectorial forms of transmission can occur through organ transplantation. We reviewed medical records of donors and recipients with suspected dengue in the first post-transplant week. We used serologic and molecular analysis to confirm the infection. Herein, we describe four cases of dengue virus transmission through solid organ transplantation. The recipients had positive serology and RT-PCR. Infection in donors was detected through serology. All cases presented with fever within the first week after transplantation. There were no fatal cases. After these cases, we implemented dengue screening with NS1 antigen detection in donors during dengue outbreaks, and no new cases were detected. In...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Dengue virus; Transmission; Solid organ transplantation; Screening.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100063
Registros recuperados: 6
Primeira ... 1 ... Última
 

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