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A mathematical framework for group analysis of von Willebrand factor multimeric composition following luminography BJMBR
Lopes,A.A.; Soares,R.P.S.; Maeda,N.Y..
The objective of the present study was to establish a method for quantitative analysis of von Willebrand factor (vWF) multimeric composition using a mathematical framework based on curve fitting. Plasma vWF multimers from 15 healthy subjects and 13 patients with advanced pulmonary vascular disease were analyzed by Western immunoblotting followed by luminography. Quantitative analysis of luminographs was carried out by calculating the relative densities of low, intermediate and high molecular weight fractions using laser densitometry. For each densitometric peak (representing a given fraction of vWF multimers) a mean area value was obtained using data from all group subjects (patients and normal individuals) and plotted against the distance between the peak...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Von Willebrand factor; Luminography; Densitometry; Curve fitting; Pulmonary hypertension.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100002
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Calcium bioavailability of raw and extruded amaranth grains Ciênc. Tecnol. Aliment.
Ferreira,Tania Aparecida; Arêas,José Alfredo Gomes.
Calcium bioavailability of raw and extruded amaranth grains was assessed in a biological assay in rats. Rats were fed for 28 days on diets in which raw or extruded amaranth was the only calcium source, compared to a control diet with calcium carbonate. Calcium and phosphorous levels were determined in the rats' serum during the experimental period and in the bones at the end of the experiment. Amaranth extrusion increased its calcium bioavailability, assessed by tibia and femur weights and calcium and phosphorous content of the bones. Apparent calcium absorption index, the force needed to break the bones and bone densitometry of both extruded and raw amaranth were the same, though different from the control group. The results show that amaranth can be a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Novel foods; Densitometry; Calcium absorption; Rats; Antinutritional factors.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200037
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Densitometric Analysis of the Human Ulna International Journal of Morphology
Aguado-Henche,S; Clemente de Arriba,C; Carrascosa-Sánchez,J.
This study describes the distribution of the normal bone mass in the mature human ulna, as knowledge of distribution may provide information to orthopaedic surgeons for the treatment of such fractures while advancing the understanding of the responses of osteosynthesis elements during bone repair. A random sample of 41 dry ulnas was studied with a DXA system using a Norland XR-26 densitometer. Two projections were performed, lateral and antero-posterior. In both projections five regions of interest (ROI) were selected: proximal (ROI 1), proximal-intermediate (ROI 2), intermediate (ROI 3), distal-intermediate (ROI 4) and distal (ROI 5). All statistical calculations were performed using Stat graphics Plus (version 5.1) and SPSS (version 15.0). Bone mineral...
Tipo: Journal article Palavras-chave: Densitometry; Ulna; Bone mineral density; Bone mineral content; Elbow.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300016
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Determining aflatoxins B1, B2, G1 and G2 in maize using florisil clean up with thin layer chromatography and visual and densitometric quantification Ciênc. Tecnol. Aliment.
CASTRO,Luciana de; VARGAS,Eugenia Azevedo.
A method for determining aflatoxins B1 (AFB1), B2 (AFB2),G1 (AFG1) andG2 (AFG2) in maize with florisil clean up was optimised aiming at one-dimensional thin layer chromatography (TLC) analysis with visual and densitometric quantification. Aflatoxins were extracted with chloroform: water (30:1, v/v), purified through florisil cartridges, separated on TLC plate, detected and quantified by visual and densitometric analysis. The in-house method performance characteristics were determined by using spiked, naturally contaminated maize samples, and certified reference material. The mean recoveries for aflatoxins were 94.2, 81.9, 93.5 and 97.3% in the range of 1.0 to 242 µg/kg for AFB1, 0.3 to 85mg/kg for AFB2, 0.6 to 148mg/kg for AFG1 and 0.6 to 140mg/kg for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Florisil; Maize; Thin-layer chromatography (TLC); Densitometry.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612001000100024
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Effect of 17ß-estradiol or alendronate on the bone densitometry, bone histomorphometry and bone metabolism of ovariectomized rats BJMBR
da Paz,L.H.B.C.; de Falco,V.; Teng,N.C.; dos Reis,L.M.; Pereira,R.M.R.; Jorgetti,V..
The objective of the present study was to evaluate the effect of 17ß-estradiol or alendronate in preventing bone loss in 3-month-old ovariectomized Wistar rats. One group underwent sham ovariectomy (control, N = 10), and the remaining three underwent double ovariectomy. One ovariectomized group did not receive any treatment (OVX, N = 12). A second received subcutaneous 17ß-estradiol at a dose of 30 µg/kg for 6 weeks (OVX-E, N = 11) and a third, subcutaneous alendronate at a dose of 0.1 mg/kg for 6 weeks (OVX-A, N = 8). Histomorphometry, densitometry, osteocalcin and deoxypyridinoline measurements were applied to all groups. After 6 weeks there was a significant decrease in bone mineral density (BMD) at the trabecular site (distal femur) in OVX rats. Both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rat; 17ß-estradiol; Alendronate; Densitometry; Histomorphometry.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800007
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Newly Formed Bone in Mandible Decortication Experimental Model Using rhBMP-2 Evaluated by Densitometric Study International Journal of Morphology
Issa,João Paulo Mardegan; Tiossi,Rodrigo; Watanabe,Plauto Christopher Aranha; Siéssere,Selma; Regalo,Simone Cecílio Hallak; Lopes,Ruberval Armando; Di Matteo,Miguel Angel Sala; Iyomasa,Mamie Mizusaki; Defino,Helton Luiz Aparecido.
The purpose of this study was to evaluate the newly formed bone, comparing two different carriers for rhBMP-2, using the decortication and nondecorticatication surgical technique in Wistar rat mandibles, evaluated by radiographic densitometry method. It was used fifty six animals according to specific treatment, which were sacrificed after 3 and 6 weeks after this. It was concluded that the decortication surgical technique was able to optimize the osteoinduction properties of the rhBMP-2, independently of the material carrier used and the period of time, according to radiographic densitometry technique.
Tipo: Journal article Palavras-chave: Radiography; Densitometry; Mandible; RhBMP-2; Bone.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100014
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Risk factors for decreased bone density in premenopausal women BJMBR
Krahe,C.; Friedman,R.; Gross,J.L..
Osteoporosis is a major health problem. Little is known about the risk factors in premenopause. Sixty 40-50-year old patients with regular menses were studied cross-sectionally. None of the patients were on drugs known to interfere with bone mass. Patients answered a dietary inquiry and had their bone mineral density (BMD) measured. The Z scores were used for the comparisons. A blood sample was taken for the determination of FSH, SHBG, estradiol, testosterone, calcium and alkaline phosphatase. Calcium and creatinine were measured in 24-h urine. A Z score less than -1 was observed for the lumbar spine of 14 patients (23.3%), and for the femur of 24 patients (40%). Patients with a Z score less than -1 for the lumbar spine were older than patients with a Z...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteoporosis; Bone loss; Premenopause; Osteopenia; Densitometry.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900003
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Two-dimensional ammonium distribution in sediment pore waters using a new colorimetric diffusive equilibration in thin-film technique ArchiMer
Metzger, Edouard; Barbe, Anthony; Cesbron, Florian; Thibault De Chanvalon, Aubin; Jauffrais, Thierry; Jézéquel, Didier; Mouret, Aurélia.
This study presents a new gel based technique to describe the pore water ammonium distribution through the sediment-water interface in two dimensions at a millimeter scale. The technique is an adaptation of the classical colorimetric method based on the Berthelot's reaction. After the thin film of the gel probe was equilibrated by diffusion either in standard solutions or in pore waters, a colorimetric reagent gel was set on the gel probe, allowing development of the characteristic green color. A flatbed scanner and subsequent densitometry image analysis allowed to determine the concentration distribution of ammonium. The gel probe was tested in the laboratory for two media, deionized water and seawater, within the range 0–3000 μM in NH4+. Detection limit...
Tipo: Text Palavras-chave: Biogeochemistry; High-resolution; Porewater; DET; Densitometry.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00474/58557/61112.pdf
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