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Behavior of coffee seeds to desiccation tolerance and storage. Repositório Alice
ABREU, L. A. de S.; VEIGA, A. D.; VON PINHO, E. V. de R.; MONTEIRO, F. F.; ROSA, S. D. V. F. da.
The technology developed by breeding programs is applied to coffee seeds; however, after processing and drying, they lose viability within a short period of time, thus making storage unsuitable. The objective of this research was to evaluate the quality of coffee seeds submitted to different drying methods and moisture contents during storage. The coffee seeds were submitted to conventional drying (slow shade drying) and fast drying in a static drier until they reached a moisture content of 40, 20, 1 2 and 5%. After this process, the seeds were stored in a cold chamber for 12 months, and seed quality was evaluated before and during storage by the germination test, electrophoretic patterns of heat resistant proteins, and the activity of isoenzyme systems....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Desiccation tolerance; Estresse oxidativo; Tolerância à dessecação; Coffea arabica; Desitratação; Longevidade; Longevity; Coffea arabica; Oxidative stress.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1039780
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Changes in gene expression during drying and imbibition of desiccation sensitive Magnolia ovata (A. St.-Hil.) spreng. seeds Rev. bras. sementes
José,Anderson C.; Ligterink,Wilco; Davide,Antonio Claudio; Silva,Edvaldo A. Amaral da; Hilhorst,Henk W.M..
Seeds of Magnolia ovata were dried to different water contents to assess the viability and transcript abundance of genes related to seed development, cell cycle, cytoskeleton and desiccation tolerance.The expression of development, cell cycle and cytoskeleton relative genes (ABI3, CDC2-like and ACT2) alone could not explain the germination behaviour of M. ovata seeds in relation to drying damage. Irrespective of their initial water content, the seeds performed in the same way during the initial period of germination and the deleterious effects of desiccation only occurred in later stages. Expression of PKABA1, sHSP17.5 and 2-Cys-PRX did not show a relationship with desiccation. However, the expression patterns of PKABA1 and sHSP17.5 suggested the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation tolerance; Drying; Gene expression; Magnolia ovata.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222009000100030
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Comparative transcriptome profile of the leaf elongation zone of wild barley (Hordeum spontaneum) eibi1 mutant and its isogenic wild type Genet. Mol. Biol.
Zhou,Qin; Wang,Aidong; Duan,Ruijun; Yan,Jun; Zhao,Gang; Nevo,Eviatar; Chen,Guoxiong.
Abstract The naturally occurring wild barley mutant eibi1/hvabcg31 suffers from severe water loss due to the permeable leaf cuticle. Eibi1/HvABCG31 encodes a full ATP-binding cassette (ABC) transporter, HvABCG31, playing a role in cutin deposition in the elongation zone of growing barley leaves. The eibi1 allele has pleiotropic effects on the appearance of leaves, plant stature, fertility, spike and grain size, and rate of germination. Comparative transcriptome profile of the leaf elongation zone of the eibi1 mutant as well as its isogenic wild type showed that various pathogenesis-related genes were up-regulated in the eibi1 mutant. The known cuticle-related genes that we analyzed did not show significant expression difference between the mutant and wild...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ABC transporter; Defense genes; Desiccation tolerance; Plant cuticle; RNA-Seq.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500834
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Comportamento fisiológico de sementes de aroeira (Myracrodruon urundeuva Fr. All.) em condições de armazenamento. Repositório Alice
MEDEIROS, A. C. de S.; SMITH, R.; PROBERT, R.; SADER, R..
O desaparecimento de espécies vegetais de relevante importância sócioeconômica pela ação do homem, exige, com urgência, o estabelecimento de eficazes tecnologias voltadas para a conservação de genes para as futuras gerações. O objetivo deste trabalho foi o de fornecer subsídios básicos sobre o comportamento fisiológico das sementes de aroeira (Myracrodruon urundeuva Fr.All. syn. Astronium urundeuva (Fr. All.) Engl.), espécie madeireira e medicinal, visando sua conservação a longo prazo, em bancos de germoplasma. Especificamente, buscou-se o estudo da viabilidade das sementes de M. urundeuva, quando submetidas à secagem (5,9% e 6,0% de umidade, base úmida), reidratação (12,2% e 13,6%, base úmida), baixa (2°C e ?13°C) e ultra-baixa temperaturas (-20°C;...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Astronium urundeuva; Semente; Ortodoxa; Desidratação; Preservação biológica; Orthodox seeds; Desiccation tolerance; Biological preservation.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/280589
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Desiccation effects on germination and vigor of King palm seeds Horticultura Brasileira
Martins,Cibele C.; Bovi,Marilene L. A.; Nakagawa,João.
The desiccation tolerance of Archontophoenix alexandrae (Wendl. &amp; Drude) seeds was determined and the most sensitive vigor test for assessing seed deterioration of this species was identified. Mature fruits were harvested in the palm collection of the Instituto Agronomico in Campinas, Brazil. Depulped fruits were transported in impermeable packages to the Faculdade de Agronomia in Botucatu, where the seeds were dried. As the seed moisture decreased, germination, seedling length, electrical conductivity and moisture were measured. The seeds of A. alexandrae are recalcitrant, with high germination percentage (over 67%) when undried (47% seed moisture). Lowering seed moisture below 31.5% reduced the germination rate significantly (<52.5%). Total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Archontophoenix alexandrae; Alexandra palm; Seed deterioration; Electrical conductivity; Recalcitrance; Desiccation tolerance; Critical moisture; Lethal moisture.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362003000100019
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Ecophysiology of coconut palm under water stress Braz. J. Plant Physiol.
Gomes,Fábio P.; Prado,Carlos H.B.A..
Coconut palm is of great social and economic importance for millions of people in the tropics and subtropics. Drought is one of the main environmental factors that limit coconut productivity. In this review, physiological and morphological data are presented in an integrated perspective to provide a holistic view of the behavior of coconut trees facing water deficit under both field and controlled conditions. Great capacity to produce homorhizic roots capable of searching continuously for water and nutrients and high protoplasmic tolerance in leaves allows the coconut tree to endure water deficit. High membrane stability, osmoprotection, osmoregulation and enhanced activity of antioxidant enzymes are characteristics often found in leaves of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocus nucifera; Desiccation tolerance; Leaf gas exchange; Leaf water relations; Net photosynthesis; Osmotic potential; Water potential; Water-use efficiency.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000400008
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Effects of different drying rates on the physiological quality of Coffea canephora Pierre seeds Braz. J. Plant Physiol.
Rosa,Sttela Dellyzete Veiga Franco da; Brandão Júnior,Delacyr da Silva; Von Pinho,Édila Vilela de Resende; Veiga,André Delly; Silva,Luiz Hildebrando de Castro e.
Desiccation tolerance in seeds depends on the species, development stage and drying conditions, especially the water removal rate. Coffea seeds are considered of intermediate performance, because they tolerate relative dehydration compared to orthodox seeds and are sensitive to low temperatures. The objective of this study was to verify the effect of different drying rates on the viability and storability of Coffea canephora seeds. A complete randomized experimental design was used, in a factorial 3 x 5 x 2 design, with three drying rates (fast, intermediate and slow), five final mean water contents after drying (51, 42, 33, 22 and 15 %) and two storage temperatures (10 and 20°C). The germination and seed vigor assessments, using radicle protrusion,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee tree; Desiccation tolerance; Seeds; Seed storage.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200002
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Evolution of research on recalcitrant seeds Scientia Agricola
Barbedo,C.J.; Bilia,D.A.C..
Some comments about seeds that show sensitivity to desiccation and short viability period, called recalcitrants, were made. Further studies showed that there was a gradient of sensitivity to desiccation and an intermediate class was proposed. The research demonstrated different factors related to desiccation tolerance such as ABA, proteins and sugars. It was analyzed the research of recalcitrant seeds in Brazil, that started around 1950 and nowadays the major aspects studied are recalcitrant seeds identification, seed low temperature and drying tolerance, storage capacity under different rooms and packages, chemical composition and viability during storage, fungicide treatment efficiency on seed conservation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed; Recalcitrant; Desiccation tolerance; Brazil.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500022
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Gene expression of coffee seed oxidation and germination processes during drying. Repositório Alice
SANTOS, G. C.; PINHO, E. V. R. von; ROSA, S. D. V. F. da.
Coffee (Coffea arabica L.) seeds are sensitive to desiccation and oxidative stress during drying processes. We investigated the effect of drying and moisture levels on germination-related gene expressions associated with enzymatic systems that prevent oxidative stress in coffee seeds. Coffee seeds collected at physiological maturity were subjected to slow and quick drying to 40, 30, 20, and 12% moisture levels (wet basis), and as the control, seeds without drying were used. The seeds? physiological quality was calculated as percentage of normal seedlings at 15 and 30 days, normal vigorous seedlings at 30 days, and cotyledonary leaves at 45 days. The isoenzymes esterase, catalase (CAT), peroxidase (POX), and endo-?-mannanase expressions were...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Desiccation tolerance; Endo-?-mannanase; Seed aging; Café; Isozymes.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1039768
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Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds BABT
José,Anderson Cleiton; Silva,Edvaldo Aparecido Amaral da; Davide,Antonio Claudio; Toorop,Peter.
The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation tolerance; Forest seed; Legumin; Proteomics.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300006
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Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation BABT
Mello,Juliana Iura de Oliveira; Barbedo,Claudio José; Salatino,Antonio; Figueiredo-Ribeiro,Rita de Cássia Leone.
Considering the importance of water content for the conservation and storage of seeds, and the involvement of soluble carbohydrates and lipids for embryo development, a comparative study was carried out among the seeds of Inga vera (ingá), Eugenia uniflora (pitanga), both classified as recalcitrant, and Caesalpinia echinata (brazilwood) and Erythrina speciosa (mulungu), considered as orthodox seeds. Low concentrations of cyclitols (0.3-0.5%), raffinose family oligosaccharides (ca. 0.05%) and unsaturated fatty acids (0-19%) were found in the seeds of ingá and pitanga, while larger amounts of cyclitols (2-3%) and raffinose (4.6-13%) were found in brazilwood and mulungu, respectively. These results, in addition to higher proportions of unsaturated fatty acids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation tolerance; Fatty acids; Seeds; Starch; Sugars.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400019
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Reversible in vivo cellular changes occur during desiccation and recovery: Desiccation tolerance of the resurrection filmy fern Hymenophyllum dentatum Cav. Gayana Botánica
Bravo,Soraya; Parra,María José; Castillo,Rosario; Sepúlveda,Francisco; Turner,Aileen; Bertín,Ariana; Osorio,Germán; Tereszczuk,Joanna; Bruna,Carola; Hasbún,Rodrigo.
ABSTRACT The present work explores in vivo physiological, morphological and chemical features during full hydration, desiccation and rehydration of the filmy fern Hymenophyllum dentatum with two main objectives: 1) to get further insight about the mechanisms underlying its desiccation tolerance, and 2) to understand how this plant manages mechanical stress induced by water loss and recovery. With these purposes, physiological (relative water content and F /F chlorophyll fluorescence parameter), morphological (Confocal Laser Scanning Microscopy and 3D reconstruction) and chemical (FT- IR microspectroscopy) data were obtained and compared between fully hydrated, desiccated and rehydrated tissues of H. dentatum. Remarkable changes in cell architecture and...
Tipo: Journal article Palavras-chave: Hymenophyllum; Desiccation tolerance; Confocal 3D microscopy; FT-IR microspectroscopy; Rapid dehydration/rehydration.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000200402
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The influence of moisture on the in vitro embryo germination and morphogenesis of babassu (Orbignya phalerata Mart.) - doi: 10.4025/actasciagron.v34i4.13429 Agronomy
Silva, Marcus Vinícius Vieira da; Instituto Federal Goiano; Sales, Juliana de Fátima; Instituto Federal Goiano; Silva, Fabiano Guimarães; Instituto Federal Goiano; Neto, Aurélio Rubio; Instituto Federal Goiano; Alberto, Paula Sperotto; Instituto Federal Goiano; Pereira, Flávia Dionísio; IFGoiano - Campus Rio Verde.
The objective of this study was to evaluate the physiological quality of babassu (Orbignya phalerata Mart.) embryos as a function of desiccation. The fruits were placed in a convection oven at 37±2°C for 0, 3, 6, 9, or 12 days. After these periods, the moisture contents of the fruits and seeds were determined. A reduction in the water content occurred more rapidly in the fruits than in the seeds. The decrease in seed moisture content from 12.59% (on the basis of fresh weight) to 4.56% did not affect the percentage of germinated zygotic embryos; however, we did observe a decline in the vigor as the seed moisture content decreased. Embryos with a 4.56% moisture content developed into seedlings of shorter lengths after 30, 60, and 90 days of in vitro growth....
Tipo: Pesquisa Empírica Palavras-chave: 5.01.03.00-8 Arecaceae; Desiccation tolerance; Tissue culture; Fitotecnia.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13429
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Water potential and viability of seeds of Eugenia (Myrtaceae), a tropical tree species, based upon different levels of drying BABT
Delgado,Liliana Ferreira; Barbedo,Claudio José.
The aim of the present work was to study the water potential and viability of Eugenia based upon different levels of drying. The seeds showed high water content at shedding and were sensitive to desiccation; however, the level of this sensitivity (water content) varied among the species. Five target drying levels were defined according to the critical levels of hydration. Results showed that water potential seemed to be more reliable than water content. It was hypothesize that the differences in desiccation tolerance, as well as recalcitrance, among the seeds of different species could be related to the maturity level of these seeds during shedding from the mother plant. Consequently, this could be a result of the evolution on how far the maturation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation tolerance; Drying; Seed water relations; Recalcitrance.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400014
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