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Registros recuperados: 86
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A polygenic hypothesis for sex determination in the European sea bass - Dicentrarchus labrax ArchiMer
Vandeputte, Marc; Dupont Nivet, M; Chavanne, H; Chatain, Beatrice.
Polygenic sex determination, although suspected in several species, is thought to be evolutionarily unstable and has been proven in very few cases. In the European sea bass, temperature is known to influence the sex ratio. We set up a factorial mating, producing 5.893 individuals from 253 full-sib families, all reared in a single batch to avoid any between-families environmental effects. The proportion of females in the offspring was 18.3%, with a large variation between families. Interpreting sex as a threshold trait, the heritability estimate was 0.62 +/- 0.12. The observed distribution of family sex ratios was in accordance with a polygenic model or with a four-sex-factors system with environmental variance and could not be explained by any genetic...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Fish; Growth; Heritability; Sex ratio.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3526.pdf
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A shortcut method for sexing juvenile European sea bass, Dicentrarchus labrax L. ArchiMer
Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice.
The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable...
Tipo: Text Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf
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Adaptation de la méthode peptide-spot à la recherche de sites, de la protéine RNA2 du nodavirus, reconnus par différents sérums de loup (Dicentrarchus labrax). ArchiMer
Coeurdacier, Jean-luc.
Spot-Synthesis technique was applied to study protein frame or to develop antibodies with specific characteristics for use in diagnostic or research on human or mammals. In this work this technique was used to find sites on RNA2 protein of Nodavirus which are immunogenic for sea bass (Dicentrarchus labrax). It aimed to achieve synthetic peptide vaccine production against this virus. One hundred and nine overlapping peptides, of 15 amino acids frameshifted by three residues, representing the complete RNA2 protein sequence of Sb1 and Sb2 nodavirus were synthesized on a cellulose membrane by the Spot-Technique. The set of membrane bound peptides was probed by incubation with sea-bass or rabbit serum. Anti sea bass or anti rabbit alkaline phosphatase...
Tipo: Text Palavras-chave: Peptides synthétiques; Vaccin; Peptid-spot; Séquence immunogène; Dicentrarchus labrax; Synthetic peptides; Vaccine; Immunogenicity; Dicentrarchus labrax...
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00157/26810/24919.pdf
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An early-life hypoxia event has a long-term impact on protein digestion and growth in European sea bass juvenile. ArchiMer
Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy.
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group. We examined...
Tipo: Text Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf
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Approche du regime alimentaire des juveniles de bars et de limandes en baie de Somme. ArchiMer
Leaute, Jean-pierre.
Stomach contents of juveniles of sea bass (Dicentrarchus labrax ) and common dab (Limanda limanda ) collected during September 1982 from Bay of Somme and the neighbor coast to Penly, were examined. The most part of the diet of young sea bass are the crustaceans, Sedentary polychaetes appear to be the major food item for common dab. For these two young predator species, food preferences tend to change early: cumaceans, then common edible shrimp, for sea bass, Tube worm tentacles, then part of whole individuals are preferred by common dab.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Prey selection; Food preferences; Stomach content; Diets.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/publication-1789.pdf
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Approche quantitative de la fonction de nourricerie jouée par les systèmes vasières-estuaires ArchiMer
Parlier, Emmanuel.
Coastal fish populations settle more or less frequently, at various stages, estuarine systems. Tidal marshes are one of the components of these mosaics of habitats. The first part of this work describes fish assemblages through the utilisation of these systems by fishes analysed in order to propose a revised functional classification of ecological guilds. T h i s new classification is used to compare fish communities of four estuarine systems in the western coast of France: the Mont Saint- Michel Bay, the Saint-Brieuc Bay, the Loire estuary and the Aiguillon Bay. The second part aims at characterizing the nursery function as defined by Beck et al. (2001). Acombinative approach based on gut content analysis, and stable isotope analysis shows that Yo Y s e a...
Tipo: Text Palavras-chave: Isotope ratio; Otolith; Saltmarsh; Nursery; Mont Saint Michel Bay; Saint Brieuc Bay; Aiguillon Bay; Seine estuary; Loire estuary; NEA; Dicentrarchus labrax; Fish communities; Rapports isotopiques; Otolithe; Marais salés; Nourricerie; Baie du Mont Saint Michel; Baie de Saint Brieuc; Baie de l'Aiguillon; Seine; Loire; ANE; Dicentrarchus labrax; Peuplement de poissons.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-4601.pdf
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Are sex ratios in wild European sea bass (Dicentrarchus labrax) populations biased? ArchiMer
Vandeputte, Marc; Quillet, Edwige; Chatain, Beatrice.
Sex ratios in farmed European sea bass are highly biased towards males (75 to 95%), which is problematic for aquaculture. In this mini-review, we re-analyse fisheries literature data about sex ratios in wild sea bass from 13 population samples, representing altogether 4889 individuals covering the major part of the distribution range of the species. We find that as a whole, the sex ratio of wild populations is biased towards females (59.4% females, p < 0.001), but that the sex ratio of the younger fish (< 30 cm total length) is balanced (52.0% females, p = 0.15), while the sex ratio of the older fish is heavily biased towards females (69.5% females, p < 0.01). Possible causes of these differences (differential longevity, biased sampling) are...
Tipo: Text Palavras-chave: Sex ratio; Genetics; Sex determination; Fish aquaculture; Dicentrarchus labrax.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18882/16450.pdf
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Assessment of sea bass (Dicentrarchus labrax, L.) stock delimitation in the Bay of Biscay and the English Channel based on mark-recapture and genetic data ArchiMer
Fritsch, Manon; Morizur, Yvon; Lambert, E; Bonhomme, Francois; Guinand, B.
Sea bass (Dicentrarchus labrax) fisheries are becoming increasingly abundant along eastern Atlantic coastlines, especially in the ICES sub-areas IV, VII and VIII, but they are still not being managed by the European Union TAC (Total Allowed Catches) system. Scientific information about these sea bass stocks is limited, but the fishery is currently managed or modelled based upon these data, separated into the ICES divisions. Clearly, failure to manage satisfactorily the fishery may lead to a decline in stocks. We therefore investigated the structure of Atlantic sea bass populations at eight microsatellite loci in the Bay of Biscay and the English Channel and, additionally, at five selected loci in Ireland and Scotland. Genetic data showed no significant...
Tipo: Text Palavras-chave: Population structure; Microsatellite loci; Tagging; Mark recapture; Dicentrarchus labrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2401.pdf
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Associations between tissue fatty acid composition and physiological traits of performance and metabolism in the seabass (Dicentrarchus labrax) ArchiMer
Chatelier, Aurelien; Mckenzie, David; Prinet, Aurelie; Galois, Robert; Robin, Jean; Zambonino, Jose-luis; Claireaux, Guy.
Seabass were fed for 4 months with diets where the lipid was provided as either canola oil (CO), palm oil ( PO) or fish oil (FO), to generate diversity in their tissue fatty acid (FA) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. In particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (OA, 18:1n-9 and LA, 18:2n-6, respectively) from the CO and PO diets would improve maximum exercise and cardiac performance, and increase aerobic metabolic scope. This proved to be the case; exercise respirometry on bass fitted with cardiac flow probes revealed that those fed CO and PO diets had a...
Tipo: Text Palavras-chave: Diet; Fatty acid; Cardiovascular performance; Metabolism; Swimming; Dicentrarchus labrax; Seabass.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1860.pdf
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Body shape variation in meiotic gynogenetic and triploid sea bass, Dicentrarchus labrax ArchiMer
Peruzzi, Stefano; Wiborg-dahle, Stine; Haffray, Pierrick; Chatain, Beatrice; Primicerio, Raul.
A method of geometric morphometrics was used to characterize body shape variation in meiotic gynogenetic and triploid sea bass in comparison with diploid controls. A total of 13 coordinate landmark data were recorded on the profile of 34-month-old control (n = 81), gynogenetic (n = 117) and triploid (n = 131) siblings generated from two females (A and B). Three main axes of morphometric variation were consistent, and these accounted for 60.8 and 57.6% of the total variability in females A and B, respectively. There were significant shape deviations of triploid and gynogenetic fish relative to the controls, and differences were particularly evident in the progeny of one female. Overall, triploids displayed a more slender shape than diploids, whereas...
Tipo: Text Palavras-chave: Body shape variability; Morphometry; Geometry of Procrustes distance; Triploidy; Gynogenesis; European sea bass; Dicentrarchus labrax.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00025/13651/10757.pdf
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Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility ArchiMer
Abascal, F; Cosson, J; Fauvel, Christian.
Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (< 50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating...
Tipo: Text Palavras-chave: Sperm; Heavy metals; Dicentrarchus labrax; CASA.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4765.pdf
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Combined effects of dietary HUFA level and temperature on sea bass (Dicentrarchus labrax) larvae development ArchiMer
Vagner, Marie; Robin, Jean; Zambonino, Jose-luis; Person, Jeannine.
The purpose of this study was to investigate the combined effect of the incorporation of vegetable products in diet and temperature on enzymatic pathways for high unsaturated fatty acids (HUFA) desaturation in sea bass larvae. Four replicated groups were fed a low (LH; 0.8% EPA+DHA) or a high (HH; 2.2% EPA+DHA) n-3 HUFA microparticulated diet from mouth opening, six days post-hatching and were reared at 16 or 22 degrees C. The four experimental conditions (LH16, HH16, LH22 and HH22) were tested for 45 days. At the end of the experiment, body weight, total length and biomass were affected by temperature (P<0.001), while biomass as well as fresh body weight was also influenced by diet (P<0.05 and P<0.001 respectively). This always lead to the same...
Tipo: Text Palavras-chave: Desaturation; HUFA; Dicentrarchus labrax; Delta 6 desaturase; Aquaculture.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2673.pdf
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Conséquences à long terme d'une exposition précose à l'hypoxie sur la physiologie du bar européen (Dicentrarchus labrax) et de la sole commune (Solea solea) ArchiMer
Vanderplancke, Gwenaelle.
The dissolved oxygen in seawater has steadily declined in many coastal marine ecosystems in the last 50 years because of global warming and increasing coastal human activities. This decrease in oxygen in seawater causes episodes of hypoxia increasingly frequent and severe in coastal and estuarine areas. Some organisms may escape these environmental constraints by migration or dispersion. Others, with limited swimming capabilities must implement physiological regulation (phenotypic plasticity) to enable them to adapt and survive. This is particularly the case of marine fish larvae that are likely to be present in coastal nursery at the end of their development. It is now well established that the regulations implemented by organisms to cope with their...
Tipo: Text Palavras-chave: Hypoxie; Conditionnement; Programmation environnementale; Poissons marins; Changement climatique; Oxygène; Dicentrarchus labrax; Solea solea; Marine fish; Climate change; Oxygen; Hypoxia; Conditioning; Environmental programming.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00498/60938/64332.pdf
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Consistency in European seabass coping styles: A life history approach ArchiMer
Ferrari, Sebastien; Millot, Sandie; Leguay, Didier; Chatain, Beatrice; Begout, Marie-laure.
Recent years have seen a growth of interest in the consistent differences in individual behaviour over time and contexts constituting so-called “individual coping styles”. An understanding of this inter-individual variation is essential to improve our knowledge of the adaptive value of behaviour. Coping styles may have implications in diverse fields, so the development of appropriate screening methods for each species appears to be the most effective way to extend our knowledge and to incorporate behavioural responses into selection-based breeding programmes, to improve the domestication and welfare of farmed fish. We tested 30 juvenile seabass (Dicentrarchus labrax) at least twice in individual-based tests (feeding recovery in isolation, aggressiveness,...
Tipo: Text Palavras-chave: Behavioural plasticity; Repeatability; Life stages; Dicentrarchus labrax.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00257/36814/35442.pdf
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Contribution of dietary arginine to nitrogen utilisation and excretion in juvenile sea bass (Dicentrarchus labrax) fed diets differing in protein source ArchiMer
Tulli, F; Vachot, Christianne; Tibaldi, E; Fournier, Vincent; Kaushik, Sadasivam.
The role of dietary arginine in affecting nitrogen utilisation and excretion was studied in juvenile European sea bass (Dicentrarchus labrax) fed for 72 days with diets differing in protein sources (plant protein-based (PM) and fish-meal-based (FM)). Fish growth performance and nitrogen utilisation revealed that dietary Arg surplus was beneficial only in PM diets. Dietary Arg level significantly affected postprandial plasma urea concentrations. Hepatic arginase activity increased (P < 0.05) in response to dietary Arg surplus in fish fed plant protein diets; conversely ornithine transcarbamylase activity was very low and inversely related to arginine intake. No hepatic carbamoyl phosphate synthetase III activity was detected. Dietary arginine levels did...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Excretion; Arginase; Urea; Arginine.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-6317.pdf
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Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions ArchiMer
Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno.
A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied....
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf
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Développement et utilisation des marqueurs génétiques hypervariables chez les espèces marines. Rapport de synthèse 1995-1998 ArchiMer
Bonhomme, Francois; Gerard, Andre.
L'objectif scientifique de l'Unité de Recherche Marine n0 16 est de partager les savoir faire conceptuels et méthodologiques des deux équipes, pour faire aboutir des programmes appliqués ou fondamentaux dans le domaine de la génétique des organismes marins. Ces programmes concernent plus particulièrement le développement et l'utilisation des marqueurs génétiques fournis par les techniques de biologie moléculaire dans les programmes suivants: Chez les huîtres des genres Ostrea et Crassostrea : Le modèle prioritaire de l'URM sera les huîtres Crassostrea et Ostrea. Pour des raisons historiques, les recherches sur Ostrea sont plus avancées et donneront des résultats plus rapidement. 1-Sélection assistée par marqueurs chez Ostrea edulis et Crassostrea gigas...
Tipo: Text Palavras-chave: Génétique; Marqueur génétique; Biologie moléculaire; Sélection génétique; Ressource génétique; Recrutement; Bivalvia; Crassostrea; Ostrea; Pisces; Dicentrarchus labrax; Génotypage.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00043/15459/12834.pdf
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Dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in European sea bass Dicentrarchus labrax larval development ArchiMer
Gisbert, Enric; Villeneuve, Laure; Zambonino, Jose-luis; Quazuguel, Patrick; Cahu, Chantal.
We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets with different levels of EPA and DHA. Diet names indicated the percentage of EPA and DHA contained in the phospholipids (PL) and neutral lipids (NL), as follows: PL5, PL3, PL1, NL1, and NL3. Histological observations showed different patterns of lipid absorption and accumulation in the intestinal mucosa depending on the level and nature of the dietary lipid fraction. Fish fed high...
Tipo: Text Palavras-chave: Histology; Digestive system; Phospholipid; Larvae; Dicentrarchus labrax; Dietary.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-929.pdf
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Dietary probiotic live yeast modulates antioxidant enzyme activities and gene expression of sea bass (Dicentrarchus labrax) larvae ArchiMer
Tovar-ramirez, D.; Mazurais, David; Gatesoupe, J. F.; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, J. L..
The main goal of this work was to determine the effect of dietary live yeast Debaryomyces hansenii on the enzymatic antioxidative status of sea bass Dicentrarchus labrax larvae. Growth, activity and expression of the main antioxidative enzymes: catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD), and heat shock protein (HSP70) were measured in sea bass larvae at 23 and 48 days after hatching. Larvae were fed on two microdiets: group one, fed microdiet containing live yeast and the control group fed microdiet without yeast. Heat shock protein 70 showed the same expression levels in both fish larvae fed yeast and the control diet. The group fed D. hansenii showed highest growth and lower activity and expression levels of GPX and SOD...
Tipo: Text Palavras-chave: Antioxidant enzymes; Probiotics; Debaryomyces hansenii; Dicentrarchus labrax.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11299/7881.pdf
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Domestication, sélection et comportement du bar: variabilité des aptitudes comportementales et de la tolérance au stress de groupes génétiquement distincts de bar, Dicentrarchus labrax ArchiMer
Millot, Sandie.
The culture of aquatic species has grown much more rapidly in recent years than that of terrestrial species. Consequently, aquatic livestock welfare is a growing concern for society. However, unlike the many studies of terrestrial animal welfare, few scientific data exist concerning fish welfare. It is generally recognized that the aquaculture environment exposes fish to many repeated stress factors which affect their health, growth, reproductive performance and feeding behaviour. Even if stress responses do not highlight all welfare disturbances, it is generally admitted that they reveal a poor welfare. Among the solutions investigated to reduce fish stress, domestication and selection appear to be the favoured approaches. However, selection has generally...
Tipo: Text Palavras-chave: Personality; Physiological status; Growth; Swimming behaviour; Feeding behaviour; Stress tolerance; Adaptation; Welfare; Chronic stress; Acute stress; Selection; Domestication; Dicentrarchus labrax; Personnalité; Statut physiologique; Croissance; Comportement natatoire; Comportement alimentaire; Tolérance au stress; Adaptation; Bien être; Stress chronique; Stress aigu; Sélection; Domestication; Dicentrarchus labrax.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-6272.pdf
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