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Gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata) ArchiMer
Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam.
Our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. We compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata), known to be relatively tolerant to dietary carbohydrates. After cloning of partial cDNA sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (G6Pase), fructose biphosphatase (FBPase) and phosphoenolpyruvate carboxykinase (PEPCK) in the two species, we analyzed gene expressions of these enzymes 6 and 24 h after feeding with (20%) or without carbohydrates. Our data show that there is at least one gluconeogenic enzyme strongly regulated (decreased expression after feeding) in the two...
Tipo: Text Palavras-chave: Gilthead seabream; Common carp; Gluconeogenesis; Gene expression; Hepatic glucose metabolism; Dietary carbohydrate.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-668.pdf
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Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio) ArchiMer
Panserat, Stéphane; Blin, C; Medale, Francoise; Plagnes Juan, E; Breque, Jeanine; Krishnamoorthy, J; Kaushik, Sadasivam.
The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from these cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly homologous to mammalian GK exists in these fish species with similar tissue specific...
Tipo: Text Palavras-chave: Glucose phosphorylation; Dietary carbohydrate; Fish nutrition.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-669.pdf
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