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Caractérisation fonctionnelle et évolution moléculaire des gènes codant pour les facteurs d'initiation de la traduction eIF4E : des facteurs clés dans la résistance des plantes aux Potyvirus Inra
Charron, C..
L'objectif de cette étude est de décrire puis analyser et comparer le polymorphisme de séquence des gènes eIF4E (eukaryotic Initiation Factor 4E) de résistance aux potyvirus, afin de mettre en relation ce polymorphisme avec la double fonction des protéines eIF4E dans les processus cellulaires de l'hôte et dans l'interaction avec les potyvirus. La stratégie repose sur l'association d'analyses fonctionnelles et d'études d'évolution moléculaire des gènes eIF4E chez deux Solanacées (piment et tomate) et chez l'espèce modèle Arabidopsis thaliana. En ce qui concerne le rôle de eIF4E dans les processus cellulaires, la pression de sélection purificatrice forte identifiée pour l'ensemble des gènes eIF4E argumente en faveur de l'acquisition d'une spécialisation...
Tipo: Thesis Palavras-chave: GENE RECESSIF DE RESISTANCE; DIVERSITE ALLELIQUE; COEVOLUTION; SELECTION POSITIVE POTYVIRUS; EIF4E.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008384d748a&uri=/notices/prodinra1/2008/06/
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Identification of a CAPS marker in an eIF4E gene linked to Zucchini yellow mosaic virus resistance in watermelon National Institute of Agronomic Research
Ling, K.S.; Harris, K.; Meyer, J.D.F.; Levi, A.; Guner, N.; Wehner, T.C.; Havey, M.J..
Genes that encode eukaryotic initiation factors (eIF) 4E and iso(4E) have been associated with the recessively inherited resistance to potyviruses in a number of plant species. Using previously developed degenerate primers, partial eIF4E and eIF(iso)4E gene sequence regions were obtained through polymerase chain reaction of the genomic DNA templates prepared from the Zucchini yellow mosaic virus (ZYMV)-resistant U.S. Plant Introduction (PI) 595203, and the ZYMV-susceptible ‘New Hampshire Midget’ (NHM). A single nucleotide polymorphism (SNP) was identified in the intron II region of the eIF4E sequence between the resistant and susceptible parental genotypes. F2 and BC1 populations were developed using PI 595203 and NHM and each plant was phenotyped for...
Tipo: Conference Paper Palavras-chave: Citrullus; Inheritance; Recessive gene; Potyvirus; ZYMV; Disease resistance; SNP markers; CAPS marker; Molecular marker; Elisa test; Inheritance; EIF4E.
Ano: 2008 URL: http://hdl.handle.net/2174/208
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Reaction of lettuce genotypes to Lettuce mosaic virus-Most (LMV-Most) and characterization of the translation factor eIF4E PAB
Moura,Mônika Fecury; Silva,Norberto da; Hoffmann,Maria Isabel Motta; Pavan,Marcelo Agenor; Krause-Sakate,Renate.
Abstract: The objective of this work was to evaluate lettuce genotypes for their reaction to Lettuce mosaic virus (LMV; Most-type, isolate AF-199) and variations of the eukaryotic translation initiation factor eIF4E. All inoculated genotypes were susceptible to LMV, which was detected by RT-PCR using specific primer pairs. However, the accessions 169501, 169501C, 172918A, and 162499 showed late development of symptoms that appeared only on the inoculated leaves. Sequencing of the coding region of eIF4E showed that these genotypes have an eIF4E0 (mol 0 ) standard typical for their susceptibility to LMV, indicating that the phenotype found is not correlated to nucleotide variations in this translation factor.
Tipo: Info:eu-repo/semantics/article Palavras-chave: EIF4E; Potyvirus; Resistance.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100125
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