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Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 4
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A technique for the extraction and restriction endonuclease digestion of total DNA from Globodera rostochiensis and Globodera pallida second stage juveniles IRD
Burrows, P.R.; Boffey, S.A..
Tipo: Text Palavras-chave: NEMATODE; JUVENILE; EXTRACTION; ADN; TECHNIQUE; ENDONUCLEASE; ELECTROPHORESE SUR GEL; AGAROSE.
Ano: 1986 URL: http://www.documentation.ird.fr/hor/fdi:23027
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Identification of the two common alleles of the bovine k-casein locus by the RFLP technique, using the enzyme hind III Inra
Leveziel, H.; Metenier, L.; Mahe, M.F.; Choplain, J.; Furet, J.P.; Paboeuf, G.; Mercier, J.C.; Grosclaude, F..
Comme on pouvait le prédire par comparaison des séquences d’ADN complémentaire établies par STEWART al. (1984) et GORODETSKIY & KALEDIN (1987), les deux allèles communs du locus de la caséine K bovine, K-CnA et K-CnB sont identifiables par un polymorphisme de longueur des fragments de restriction, en utilisant soit Hind III, soit Taq I. Cette dernière endonucléase révèle aussi un polymorphisme du brin d’ADN portant l’allèle K-CnA. Pour la détermination des deux allèles, l’utilisation de Hind III est préférable car, d’après les données des auteurs ci-dessus, le polymorphisme détecté par cet enzyme est spécifique de la substitution d’acides aminés responsable de la différence de charge entre les deux variants de la caséine K. Si l’ADN a été...
Tipo: Journal Article Palavras-chave: CASEINE KAPPA; VARIANT GENETIQUE; POLYMORPHISME DE LONGUEUR DES FRAGMENTS DE RESTRICTION; ENDONUCLEASE; LEUCOCYTE; CHIMERISME; ALLELE; CARTE GENETIQUE.
Ano: 1988 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB8900000653012905&uri=/notices/prodinra1/2008/01/
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Mutation detection using ENDO1: application to disease diagnostics in humans and TILLING and Eco-TILLING in plants Inra
Triques, K.; Piednoir, E.; Dalmais, M.; Schmidt, J.; Le Signor, C.; Sharkey; Caboche, M.; Sturbois, B..
Background: Most enzymatic mutation detection methods are based on the cleavage of heteroduplex DNA by a mismatch-specific endonuclease at mismatch sites and the analysis of the digestion product on a DNA sequencer. Important limitations of these methods are the availability of a mismatch-specific endonuclease, their sensitivity in detecting one allele in pool of DNA, the cost of the analysis and the ease by which the technique could be implemented in a standard molecular biology laboratory.Results: The co-agroinfiltration of ENDO1 and p19 constructs into N. benthamiana leaves allowed high level of transient expression of a mismatch-specific and sensitive endonuclease, ENDO1 from Arabidopsis thaliana. We demonstrate the broad range of uses of the produced...
Tipo: Journal Article Palavras-chave: ENDONUCLEASE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200831e52dbf&uri=/notices/prodinra1/2009/11/
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Restriction endonuclease analysis and diagnosis of the granulosis virus isolated from Spodoptera littoralis Boisd. in West Africa and multiplied in Egypt IRD
Abol Ela, S.; Fédière, Gilles; Nour El Din, A.; Khamiss, O.; Salah, M..
Tipo: Text Palavras-chave: VIRUS ENTOMOPATHOGENE; INSECTE NUISIBLE; VIROSE; ETUDE COMPARATIVE; ADN; DIAGNOSTIC EPIDEMIOLOGIQUE; ENDONUCLEASE; ELECTROPHORESE SUR GEL; AGAROSE.
Ano: 1994 URL: http://www.documentation.ird.fr/hor/fdi:41348
Registros recuperados: 4
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