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Characterization of typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the classical O55 serogroup by RAPD analysis Rev. Microbiol.
Girão,Dennys M.; Bando,Sílvia Y.; Girão,Valéria Brígido de C.; Moreira-Filho,Carlos A.; Fracalanzza,Sérgio Eduardo L.; Trabulsi,Luiz R.; Monteiro-Neto,Valério.
The genetic diversity of 41 typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the serogroup O55 was analyzed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to the serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on E. coli O55 infection.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Genetic diversity; RAPD analysis.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400013
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Identificação sorológica de amostras de Escherichia coli isoladas de queijo Minas artesanal. Repositório Alice
PINTO, F. de R.; BARBOSA, M. M. C.; SOUZA, V. de; RIBEIRO, L. F.; NADER FILHO, A.; AMARAL, L. A. do..
O objetivo do estudo foi determinar a ocorrência de E. coli diarreiogênicas EPEC, EHEC e EIEC em queijos Minas Artesanal comercializados no município de Sacramento ? MG.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: EIEC; EPEC; Sorogrupo; Queijo Minas Frescal; Queijo artesanal; Leite cru; Escherichia coli.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/899702
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Les Escherichia Coli potentiellement pathogenes dans l’environnement littoral : cas des STEC et des EPEC ArchiMer
Baliere, Charlotte.
The contamination of coastal areas by potentially pathogenic enteric bacteria is of concern for the sustainability of some uses, such as shellfish farming, recreational shellfish harvesting and bathing. The contamination of these environments may occur through the land-spreading of livestock wastes, animal feces deposited on pastures, wastewaters from slaughterhouses or from municipal wastewater treatment plants. The presence of these bacteria in coastal environment may present a potential risk to human health. In fact, shellfish-borne outbreaks or human infections may occur by the consumption of shellfish from contaminated areas or the ingestion of water during bathing in contaminated waters, respectively. To date, few studies focusing on the presence and...
Tipo: Text Palavras-chave: STEC; EPEC; Coquillages; Diversité; Virulence; Persistance; STEC; EPEC; Shellfish; Diversity; Virulence; Persistence.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00312/42322/41653.pdf
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Molecular characterization and evaluation of antimicrobial susceptibility of enteropathogenic E. coli (EPEC) isolated from minas soft cheese Ciênc. Tecnol. Aliment.
Dias,Mariana Tavares; Bricio,Silvia Maria Lopes; Almeida,Davi Oliveira; Oliveira,Luiz Antônio Trindade; de Filippis,Ivano; Marin,Victor Augustus.
Foodborne disease caused by microorganisms is a problem of public health. Minas soft cheese is a national product manufactured using simple technology; it has high level of acceptance in the country making its production an important economic activity. Many microorganisms may be present in foods including the bacterium Escherichia coli (E. coli). Overall, E. coli is a harmless commensal bacterium; however, some strains may have a pathogenic potential. Several outbreaks of foodborne diseases associated with consumption of contaminated cheese have been reported, and the presence of pathogenic strains of E. coli has increased. The objective of this study was to isolate, evaluate the antimicrobial susceptibility, and characterize, by Multiplex PCR, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minas soft cheese; EPEC; Antimicrobial.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400017
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Molecular identification of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea in Londrina, Parana, Brazil BJM
Kobayashi,Renata K. T.; Saridakis,Halha Ostrensky; Dias,Angela M.G.; Vidotto,Marilda C..
In this work, the prevalence of enteropathogenic Escherichia coli (EPEC) in children in Londrina-PR, Brazil, was evaluated by means of digoxigenin-labelled DNA probes which identify the plasmid responsible for EPEC adherence factor (EAF), and virulence genes for EPEC as bundle-forming pilus (bfp) and E. coli attaching-effacing factor (eae). In addition, the isolated strains were serotyped and tested for adherence to HEp-2 cells. From 102 children with diarrhoea, 19 strains hybridized with at least one probe, and eleven of them were identified as typical EPEC because they hybridized with the three probes used, showed a localized adherence (LA) pattern, and presented no genes for enterotoxins (ST and LT) or invasion as detected by PCR. Six of the typical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Digoxigenin-labelled DNA probes; Virulence factors; Serotypes; Diarrhoea.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400007
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Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis BJM
Koga,Paula Célia Mariko; Menezes,Caroline Anunciação; Lima,Flávia Afonso; Nara,Júlia Mitico; Magalhães,Caroline Arantes; Cianciarullo,Aurora Marques; Ferreira-Júnior,Jorge Mario da Costa; Trabulsi,Luiz Rachid; Mendes-Ledesma,Meire Roberta Bresciani; Piazza,Roxane Maria Fontes.
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intimin; Phenotypical analysis; Polyclonal antibody; EPEC; EHEC.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002
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Prevalence and Characterization of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Shellfish-Harvesting Areas and Their Watersheds ArchiMer
Baliere, Charlotte; Rince, Alain; Blanco, Jorge; Dahbi, Ghizlane; Harei, Josee; Vogeleer, Philippe; Giard, Jean-christophe; Mariani-kurkdfian-, Patricia; Gourmelon, Michele.
Tipo: Text Palavras-chave: STEC; EPEC; Shellfish; Water; MLST; PFGE; Biofilms.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00298/40904/39949.pdf
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Prevalence of enteropathogenic and enterotoxigenic Escherichia Coli in foods of animal origin in southern Brazil Ciência Rural
Aleixo,José Antonio; Aver,Gladis Paim.
Prevalence of Escherichia coli in foods of animal origin from the city of Pelotas, Brazil, was determined. The ocurrence of enterotoxigenic (ETEC) and classic enteropathogenic (EPEC) serogroups among E. coli isolates was determined. E. coli was isolated from 68% of the food samples surveyed. Of 36 food samples tested, 11(30%) and 24(66%) were positive for EPEC and ETEC strains respectively. However, of 187 E. coli isolates tested, 30(16%) were EPEC compared to 75(40%) which were ETEC. The antibiotic resistance pattern revealed that the isolates were highly sensitive to all antibiotics tested.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; EPEC; ETEC; Foods; Brazil.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000200013
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Prevalence of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Wild and Pet Birds in Iran Rev. Bras. Ciênc. Avic.
Koochakzadeh,A; Askari Badouei,M; Zahraei Salehi,T; Aghasharif,S; Soltani,M; Ehsan,MR.
ABSTRACT The aim of this study was to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains and to identify the stx gene types in wild captive and companion birds. In total,657 E. coli isolates from 219 birds belonging to 38 different species were investigated for the presence of STEC and EPEC strains. It was shown that five birds (2.28%) carried strains positive for one or more of the virulence factors investigated. The results indicated that 1.8% (n=4) and 0.45% (n=1) of the birds carried STEC and EPEC strains, respectively. All STEC strains harbored the stx2f and eae genes and this finding reveals the role of other birds, in addition to pigeons, as reservoirs of STEC. The only EPEC strain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EPEC; STEC; Stx2f; Pet birds; Wild birds.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400445
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Shigatoxigenic and atypical enteropathogenic Escherichia coli in fish for human consumption BJM
Cardozo,Marita Vedovelli; Borges,Clarissa Araújo; Beraldo,Lívia Gerbasi; Maluta,Renato Pariz; Pollo,Andressa Souza; Borzi,Mariana Monezi; dos Santos,Luis Fernando; Kariyawasam,Subhashinie; Ávila,Fernando Antônio de.
ABSTRACT Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.
Tipo: Info:eu-repo/semantics/other Palavras-chave: EPEC; Fish; MLST; STEC; Virulence genes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400936
Registros recuperados: 10
Primeira ... 1 ... Última
 

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