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Registros recuperados: 31
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A transcriptome analysis of mitten crab testes (Eriocheir sinensis) Genet. Mol. Biol.
Zhang,Wei; Wan,Haolei; Jiang,Hui; Zhao,Yunlong; Zhang,Xiaowei; Hu,Songnian; Wang,Qun.
The identification of expressed genes involved in sexual precocity of the mitten crab (Eriocheir sinensis) is critical for a better understanding of its reproductive development. To this end, we constructed a cDNA library from the rapid developmental stage of testis of E. sinensis and sequenced 3,388 randomly picked clones. After processing, 2,990 high-quality expressed sequence tags (ESTs) were clustered into 2,415 unigenes including 307 contigs and 2,108 singlets, which were then compared to the NCBI non-redundant (nr) protein and nucleotide (nt) database for annotation with Blastx and Blastn, respectively. After further analysis, 922 unigenes were obtained with concrete annotations and 30 unigenes were found to have functions possibly related to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reproduction; Testis; EST; Eriocheir sinensis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100024
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Análise in silico de aquaporinas potencialmente envolvidas com o estresse hídrico nas interações café- e citros-Xylella fastidiosa. Repositório Alice
RABELO, F. R.; CARAZZOLLE, M. F.; MARTINS, N. F.; CAMPOS, M. A.; SILVA, M. S.; SILVA, A. C.; SOUZA, A. A. de; AMARAL, A. M. do; REIS, A. M. dos.
No Brasil, um dos importantes problemas enfrentados por produtores de café e citros envolve os danos causados pela bactéria Xylella fastidiosa. Esta bactéria causa o entupimento dos vasos do xilema e impede a passagem de água e nutrientes, o que causa uma condição biológica de estresse hídrico. Os genomas de citros e café têm sido investigados funcionalmente e várias seqüências expressas (ESTs) foram identificadas a partir de diversas condições biológicas. Bibliotecas de EST utilizando folhas e ramos infectados com X. fastidiosa foram construídas em citros e café, respectivamente. A análise in silico das ESTs nesses tecidos revelou genes comuns expressos em ambas as culturas. Alguns destes genes codificam para proteínas relacionados com a resposta de...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: EST; Filogenia; Aguaporina; Drought-induced protein Di19-like protein DIP; Fiber protein Fb2.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/906023
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Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa Genet. Mol. Biol.
Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A..
In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sweet orange; EST; CVC; Compatible interaction.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023
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Brazilian coffee genome project: an EST-based genomic resource Braz. J. Plant Physiol.
Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães.
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; CDNA; EST; Transcriptome.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008
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Caracterização de genes com expressão tecido-específica em raiz e folha de Coffea arabica. Repositório Alice
BRANDALISE, M.; MALUF, M. P.; GUERREIRO FILHO, O.; GONÇALVES, W.; MAIA, I. de G..
O grande número de genes que tem sido identificado nos últimos anos terá um grande impacto no melhoramento genético vegetal, principalmente pela obtenção de plantas geneticamente modificadas. Tal inovação, apesar de já ocasionar um grande impacto na agricultura mundial aumentando produtividade e diminuindo custos no cultivo, tem sido alvo de muitas críticas e, uma das principais, tem sido a expressão constitutiva dos transgenes, a qual é promovida pelas seqüências promotoras atualmente empregadas. A alternativa mais viável para substituição de tais promotores é investir na clonagem e caracterização de promotores tecido específicos. Visando selecionar genes com expressão tecido específica em café (Coffea arabica) para posterior clonagem dos promotores...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Sequência expressa; EST; Promotores tecido-específicos; Coffea arabica.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/handle/doc/909351
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Caracterização isoenzimática e morfológica de clones e introduções de alho Bragantia
Siqueira,Walter José; Medina Filho,Herculano Penna; Lisbão,Rogério Salles; Fornasier,João Baptista.
Em virtude do grande número de denominações locais para clones de alho, nem sempre correspondentes a materiais distintos, conduziu-se o presente estudo objetivando a caracterização e classificação de 72 clones e introduções de alho (Allium sativum L.), e um clone de alho-rei (A. ampeloprasum L.). Isso foi feito analisando as isoenzimas alcooldesidrogenase (ADH), esterase (EST), peroxidase (PRX) e fosfoglucoisomerase (PGI) através da técnica de eletroforese horizontal em gel de amido hidrolisado de batata. Verificou-se que os clones nacionais e introduzidos se enquadram nos grupos aqui denominados DIKA ou CJLB, respectivamente para os padrões de ADH, EST, PRX e PGI. Entretanto, os padrões CILB, CJKB e CIKB foram observados em alguns clones estrangeiros,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eletroforese; Isoenzimas ADH; EST; PRX; PGI; Alho; Caracterização de cultivares.
Ano: 1985 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051985000100032
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Crassostrea gigas ferritin: cDNA sequence analysis for two heavy chain type subunits and protein purification ArchiMer
Durand, Jean-pierre; Goudard, Françoise; Pieri, Jacques; Escoubas, Jean-michel; Schreiber, Nathalie; Cadoret, Jean-paul.
Ferritin has been shown as being the principal iron storage in the majority of living organisms. In marine species, ferritin is also involved in high-level accumulation of Po-210. As part of our work on the investigation of these radionuclides' concentration in natural environment, ferritin was searched at the gene and protein level. Ferritin was purified from the visceral mass of the oyster Crassostrea gigas by ion-exchange chromatography and HPLC. SDS-PAGE revealed one band of 20 kDa. An Expressed Sequence Tag (EST) library was screened and led to the identification of two complementary DNA (cDNA) involved in ferritin subunit expression. The complete coding sequences and the untranslated regions (UTRs) of the two genes were obtained and a 5' Rapid...
Tipo: Text Palavras-chave: Iron; IRE; EST; Oyster.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-693.pdf
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Current genomic resources in melon and other cucurbits National Institute of Agronomic Research
Garcia-Mas, J..
In recent years there has been an effort towards the development of genomic resources in several cucurbit species, mainly in melon, cucumber and watermelon. The number of available sequences for these species in public databases has increased recently. This has allowed the design of microarrays for transcriptome analysis and the discovery of SNP markers for genotyping. Other genomic tools are also available in melon as BAC libraries, mutant populations and saturated genetic maps. Some international initiatives have emerged that will probably lead to the sequencing of the complete genomes of some cucurbit species during the next years.
Tipo: Conference Paper Palavras-chave: Genomic tool; Microarray; EST; Mutants; Physical map; SNP markers; BAC libraries; Genetic maps; EST databases; Genome; QTL.
Ano: 2008 URL: http://hdl.handle.net/2174/213
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Cytology, biochemistry and molecular changes during coffee fruit development Braz. J. Plant Physiol.
De Castro,Renato D.; Marraccini,Pierre.
In commercial coffee species (Coffea arabica and Coffea canephora), fruit development is a lengthy process, characterized by tissue changes and evolutions. For example, soon after fecundation and up to mid development, the fruit is mainly constituted of the pericarp and perisperm tissue. Thereafter, the perisperm gradually disappears and is progressively replaced by the endosperm (true seed). Initially present in a "liquid" state, the endosperm hardens as it ripens during the maturation phase, as a result of accumulation of storage proteins, sucrose and complex polysaccharides representing the main reserves of the seed. The last step of maturation is characterized by the dehydration of the endosperm and the color change of the pericarp. Important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea spp; Bean development; Cell cycle; Endosperm; EST; Gene expression; Pericarp; Perisperm.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100013
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DATAMusa - a database for ortholog genes from Musa. Repositório Alice
TOGAWA, R. C.; SANTOS, C. M. R.; MILLER, R. N. G.; SOUZA JUNIOR, M. T.; MARTINS, N. F..
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Armazenamento de dados; Anotação de genes; Resposta ao stress; Musa acuminata; EST; Montagem; Genes transcritos.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/884025
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Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment ArchiMer
Li, Ronghua; Li, Qi; Cornette, Florence; Degremont, Lionel; Lapegue, Sylvie.
We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously...
Tipo: Text Palavras-chave: Pacific oyster; Crassostrea gigas; Microsatellite; EST; Multiplex PCR; Parentage assignment.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00013/12419/9999.pdf
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Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction Genet. Mol. Biol.
Freitas-Astúa,Juliana; Bastianel,Marinês; Locali-Fabris,Eliane C.; Novelli,Valdenice M.; Silva-Pinhati,Ana Carla; Basílio-Palmieri,Ana Carolina; Targon,Maria Luisa P.N.; Machado,Marcos A..
Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in the Brazilian citrus industry. This occurs because of the widespread source of inoculum and the year-round presence of the vector, the tenuipalpid mite Brevipalpus phoenicis, in citrus plants. In addition, while some Citrus species are resistant to CiLV-C, C. sinensis, the main cultivated species in the country, is extremely susceptible to the disease. The main objective of this work was to identify genes in C. sinensis cv. Pêra plants that were differentially expressed after the host was challenged with CiLV-C. In order to accomplish that, cDNA libraries were constructed from healthy and CiLV-inoculated sweet orange leaves. Two hundred and fifty-four genes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EST; Disease; CiLV; In silico hybridization; Sweet orange; Citrus sinensis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500026
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ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus Genet. Mol. Biol.
Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann.
Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008
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ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus. Infoteca-e
CASSOLI, C. S. da S.; JORGE, E. C.; PATRÍCIO, M.; ALVES, H. J.; ALMEIDA, E. A. de; LEDUR, M. C.; COUTINHO, L. L..
2007
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Frango; Hipotálamo; Glândula pituitária; EST; Expressed Sequence Tags; SNP.
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/443641
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ETIQUETAS DE SECUENCIAS EXPRESADAS DIFERENCIALES DE FRUTOS DE AGUACATE RAZA MEXICANA (Persea americana Mill. var. drymifolia) Rev. Bras. Frutic.
Sánchez-González,Enrique Ignacio; Hernández-Delgado,Sanjuana; Aguirre-Arzola,Víctor Eustorgio; Torres-Castillo,Jorge Ariel; Gutiérrez-Díez,Adriana.
RESUMEN En este estudio se desarrollaron e identificaron etiquetas de secuencias expresadas diferencialmente (ESTs) de frutos inmaduros de Persea americana Mill. var. drymifolia del estado de Nuevo León, México. Diez genotipos con frutos de forma y tamaño diferentes fueron seleccionados para generar ESTs por la técnica de despliegue diferencial.Se obtuvieron en total 393 fragmentos diferenciales amplificados, 82 fragmentos diferenciales fueron secuenciados y editados para identificación y comparación en las bases de datos para nucleótidos y proteínas del NCBI. Cuarenta secuencias mostraron similitud significativa con secuencias de ARNm y/o secuencias de proteínas hipotéticas o predichas pertenecientes a P. americana y/o a otros géneros. Algunas secuencias...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aguacate; Raza Mexicana; Despliegue diferencial; EST.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100102
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Exploiting a wheat EST database to assess genetic diversity Genet. Mol. Biol.
Karakas,Ozge; Gurel,Filiz; Uncuoglu,Ahu Altinkut.
Expressed sequence tag (EST) markers have been used to assess variety and genetic diversity in wheat (Triticum aestivum). In this study, 1549 ESTs from wheat infested with yellow rust were used to examine the genetic diversity of six susceptible and resistant wheat cultivars. The aim of using these cultivars was to improve the competitiveness of public wheat breeding programs through the intensive use of modern, particularly marker-assisted, selection technologies. The F2 individuals derived from cultivar crosses were screened for resistance to yellow rust at the seedling stage in greenhouses and adult stage in the field to identify DNA markers genetically linked to resistance. Five hundred and sixty ESTs were assembled into 136 contigs and 989 singletons....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodiversity; EST; Genetic diversity; Triticum; Yellow rust.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400019
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Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae) Neotropical Ichthyology
Costa,Fábio L. S.; Lima,Maria E. De; Pimenta,Adriano C.; Figueiredo,Suely G.; Kalapothakis,Evanguedes; Salas,Carlos E..
Species of the family Scorpaenidae are responsible for accidents and sporadic casualties by the shore they inhabit. The species Scorpaena plumierifrom this family populate the Northeastern and Eastern coast of Brazil causing human envenomation characterized by local and systemic symptoms. In experimental animals the venom induces cardiotoxic, hypotensive, and airway respiratory effects. As first step to identify the venom components we isolated gland mRNA to produce a cDNA library from the fish gland. This report describes the partial sequencing of 356 gland transcripts from S. plumieri. BLAST analysis of transcripts showed that 30% were unknown sequences, 17% hypothetical proteins, 17% related to metabolic enzymes, 14% belonged to signal transducing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDNAs; EST; Glands; Lectin; Scorpionfish; Toxins.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400871
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Expression and polymorphism of watermelon fruit ESTs National Institute of Agronomic Research
Levi, A.; Wechter, W.P.; Harris, K.R.; Davis, A.R.; Fei, Z.; Giovannoni, J.J.; Trebitsh, T.; Salman, A.; Hernandez, A.; Thimmapuram, J.; Tadmor, Y.; Portnoy, V.; Katzir, N..
Over 8,000 ESTs were generated for watermelon and were assembled into 4,700 EST-unigenes (http://www.icugi.org). Microarray and Real-Time PCR analyses were used to examine differential expression of 832 of these EST-unigenes in developing and ripening watermelon fruit. RNA was isolated from watermelon fruits(in three sequential stages of development and ripening) and from young leaves of field grown plants (during three consecutive years). The microarray analysis identified 335 EST-unigenes differentially expressed (by at least two-fold when compared to leaf) in watermelon fruit during the early, ripening, or mature stage. A considerable number of these watermelon ESTs are associated with vascular system differentiation and development, ethylene...
Tipo: Conference Paper Palavras-chave: Watermelon; Microarray; Polymorphism; PCR analysis; Expressed sequence tag; EST; Gene expression.
Ano: 2008 URL: http://hdl.handle.net/2174/216
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Expression profile of oxidative and antioxidative stress enzymes based on ESTs approach of citrus Genet. Mol. Biol.
Peroni,Luis Antonio; Ferreira,Renato Rodrigues; Figueira,Antonio; Machado,Marcos Antonio; Stach-Machado,Dagmar Ruth.
Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS). Plants are adequately protected by the presence of multiple antioxidative enzymes in the cytosol and also in the different cell organelles such as chloroplasts, mitochondria, and peroxisomes. Traditionally, ROS were considered to be only a toxic byproduct of aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control many different physiological processes such as abiotic and biotic stress response, pathogen defense and systemic signaling. The search results using the Citrus Genome Program in Brazil (CitEST) for oxidative stress...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Genome; EST; Reactive oxygen species; Oxidative stress enzyme.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500016
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Genes expressos nas raízes de duas linhagens de milho tolerantes sob estresse de alumínio. Repositório Alice
JARDIM, S.N.; GOMES, E. A.; LANA, U. G. P.; PAIVA, E.; GUIMARAES, C. T.; PURCINO, A. A. C.; CARNEIRO, N. P..
2004
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Aluminio; Estresse; EST.
Ano: 2004 URL: http://www.alice.cnptia.embrapa.br/handle/doc/488076
Registros recuperados: 31
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