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An Electron Microscopic Study of the Antifertility Potential of Rosemary (Rosmarinus officinalis L.) in Male Albino Rats International Journal of Morphology
El-Din,Rania A. Salah; El-Shahat,Abd El-Rahman; Elmansy,Rasha Ahmed.
The present work was aimed at studying the antifertility potential of the commonly used herb, rosemary in the male albino rats using electron microscopy as the method of investigation. Ethanolic extract of the rosmary prepared and administered orally in two different doses for a period of three months to the animals. At the end of the experiment animals were sacrificed and testes removed. Sections for the electrone microscopy prepared and changes were observed. The present results showed evident microscopic changes in the testis of the animals received higher dose of the drug. Most of the seminiferous tubules were compressed, having irregular basement membrane and devoid of any spermatogenic cells. The present work revealed a clear morphological evidence...
Tipo: Journal article Palavras-chave: Antifertility potential; Rosemary; Rosmarinus officinalis L; Electron microscopy; Male albino rats.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200051
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An improved purification procedure for Leishmania RNA virus (LRV) BJM
Souza,Marcos Michel de; Manzine,Livia Regina; Silva,Marcos Vinicius G. da; Bettini,Jefferson; Portugal,Rodrigo Vilares; Cruz,Angela Kaysel; Arruda,Eurico; Thiemann,Otavio Henrique.
Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania guyanensis; Leishmania RNA virus; LRV; Virus isolation; Electron microscopy.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200044
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Antifungal activity of the ethanolic extracts of Punica granatum L. and evaluation of the morphological and structural modifications of its compounds upon the cells of Candida spp. BJM
Anibal,Paula Cristina; Peixoto,Iza Teixeira Alves; Foglio,Mary Ann; Höfling,José Francisco.
Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of Punica granatum, known as pomegranate, had their antifungal activity tested against Candida spp. The ethanolic crude extracts were analyzed by Mass Spectrometry and yielded many compounds such as punicalagin and galladydilacton. The extracts from the pericarp and peel showed activity against Candida spp., with MICs of 125 µg/mL. The effect of pericarp and peel extracts upon the morphological and structure of C. albicans and C. krusei were examined by scanning and transmission electron microscopy, with the visualization of an irregular membrane and hyphae, formation of vacuoles and thickening of the cell wall. The data obtained revealed potential...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Punica granatum; Punicalagin; Antifungal activity; Candida spp.; Electron microscopy.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300027
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Biosíntesis de lignina y comportamiento productivo en tres pastos de clima templado. Colegio de Postgraduados
Vargas Romero, Juan Manuel.
Tres especies de gramíneas de clima templado, el híbrido Festulolium y sus progenitores Festuca arundinacea y Lolium perenne, se evaluaron en invernadero para determinar el crecimiento, fracciones de la fibra y características anatómicas de cada especie. Además se extrajo el ADN de las tres gramíneas para realizar un análisis molecular y calcular correlaciones entre los marcadores y las variables analizadas. De acuerdo con los resultados, el Festulolium fue distinto a F. arundinacea y L. perenne en el número de láminas por tallo; respecto a la abundancia relativa de lignina, área de xilema y floema, el contenido fue similar entre Festulolium y L. perenne, mientras que los valores de densidad de tricomas en el haz fueron semejantes entre Festulolium y F....
Palavras-chave: Paredes celulares; FDN; FDA; Estomas; Tricomas; Microscopía electrónica; Festulolium; Cell walls; NDF; FDN; Trichomes; Electron microscopy; Ganadería; Doctorado; Festuca arundinacea; Lolium perenne.
Ano: 2012 URL: http://hdl.handle.net/10521/744
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Boron affects the growth and ultrastructure of castor bean plants Scientia Agricola
Silva,Denis Herisson da; Rossi,Monica Lanzoni; Boaretto,Antonio Enedi; Nogueira,Neusa de Lima; Muraoka,Takashi.
The cultivation of oleaginous plants like the castor bean guarantees employment for agricultural families and can contribute in energy and chemical sectors, especially in the northeastern semi-arid regions of Brazil. Boron (B) deficiency is a widespread nutritional disorder despite the fact that various anthropogenic sources with high B content may increase soil B to toxic levels for plants. The present study was designed to investigate the ultrastructural effects of boron deficiency and toxicity on castor bean plants which were grown under greenhouse condition using plastic containers with 10 L of nutrient solution. Boron treatments comprised: control (no B); 0.27 mg L-1, 5.40 mg L-1 B pots (one plant per pot), tested in a completely randomized design...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electron microscopy; Middle lamellae; Micronutrient; Xylem; Starch.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000600014
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Centromere domain organization and histone modifications BJMBR
Bjerling,P.; Ekwall,K..
Centromere function requires the proper coordination of several subfunctions, such as kinetochore assembly, sister chromatid cohesion, binding of kinetochore microtubules, orientation of sister kinetochores to opposite spindle poles, and their movement towards the spindle poles. Centromere structure appears to be organized in different, separable domains in order to accomplish these functions. Despite the conserved nature of centromere functions, the molecular genetic definition of the DNA sequences that form a centromere in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, in the fruit fly Drosophila melanogaster, and in humans has revealed little conservation at the level of centromere DNA sequences. Also at the protein level few...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Centromere; Kinetochore; Histone acetylation; Electron microscopy.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500001
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Coat protein and RNAs of Cole latent virus are not present within chloroplasts of Chenopodium quinoa-infected cells Trop. Plant Pathol.
Belintani,Priscila; Gaspar,José O..
Cole latent virus (CoLV), genus Carlavirus, was studied by electron microscopy and biochemical approaches with respect both to the ultrastructure of the Chenopodium quinoa infected cells and to its association with chloroplasts. The CoLV was observed to be present as scattered particles interspersed with membranous vesicles and ribosomes or as dense masses of virus particles. These virus particles reacted by immunolabelling with a polyclonal antibody to CoLV. Morphologically, chloroplasts, mitochondria and nuclei appeared to be unaltered by virus infection and virus particles were not detected in these organelles. However, virus particle aggregates were frequently associated with the outer membrane of chloroplasts and occasionally with peroxisomes....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brassica; Electron microscopy; Carlavirus; Virus replication; Plant virus.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000100012
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Correlative microscopy of Purkinje cells Biocell
Castejón,Orlando J..
The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerebellar cells; Synaptic contacts; Electron microscopy; Immunohistochemistry.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100001
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Cytopathological characterization of Mal de Río Cuarto virus in corn, wheat and barley Trop. Plant Pathol.
ARNEODO,JOEL D.; LORENZO,EUGENIA; LAGUNA,IRMA G.; ABDALA,GUILLERMINA; TRUOL,GRACIELA A..
The Mal de Río Cuarto disease is caused by Mal de Río Cuarto virus (MRCV) transmitted by Delphacodes kuscheli. Comparative studies were carried out on the cytopathological alterations produced by MRCV in corn (Zea mays), wheat (Triticum aestivum) and barley (Hordeum vulgare), as seen with a transmission electron microscope. Corn plants were infected with viruliferous D. kuscheli collected from the endemic disease area (i.e. Río Cuarto County, Córdoba, Argentina). For the viral transmission to small grain cereal plants, laboratory rared insects were used. In this case, the inoculum source was wheat and barley plants infected with MRCV isolate grown in a greenhouse. Leaf samples with conspicuous symptoms were collected: enations and size reduction in corn;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Virus characterization; Electron microscopy; Fijivirus; Reoviridae; Cereals.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300010
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Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil Trop. Plant Pathol.
NICKEL,OSMAR; FAJARDO,THOR V. M.; ARAGÃO,FRANCISCO J. L.; CHAGAS,CÉSAR M.; KUHN,GILMAR B..
An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GVB; Vitis spp.; Vitivirus; Electron microscopy; ELISA; Western blot; GVA.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300007
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Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy) ArchiMer
Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G..
The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,...
Tipo: Text Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf
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Detection of viral particles in feces of young dogs and their relationship with clinical signs Rev. Microbiol.
Amo,Alicia N. del; Aprea,Adriana N.; Petruccelli,Miguel A..
Gross and light microscopic studies of 100 stool specimens of young dogs were carried out. Viral particles were detected in 31% of the analized samples using negative contrast electron microscopic diagnostic technique. Parvo-like virus, corona-like virus and other non-identified particles were observed in 17%, 7% and 2% of the samples, respectively. Parvo-like and corona-like viruses were found together in 5% of the samples. More than half (58.82%) of the positive parvo-like virus specimens were from dogs aged between 6 weeks and 6 months. 42.85% of the corona-like virus positive samples were detected in dogs between 6 weeks and 6 months and a similar percentage was found in dogs older than six months of age. Dual infections with parvo-like and corona-like...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parvo-like virus; Corona-like virus; Diarrhea; Dogs; Electron microscopy.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300009
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Determinación del agente causal de la pudrición del cogollo del Agave tequilana Weber var. Azul en el estado de Jalisco. Colegio de Postgraduados
Cen Caamal, Julio César.
En los últimos años la superficie cultivada de Agave tequilana Weber var. Azul en el estado de Jalisco ha disminuido, debido a la presencia de una enfermedad cuyos síntomas se manifiestan como pudrición blanda del cogollo y muerte de la planta. El objetivo principal fue determinar el agente causal de esta enfermedad. Para ello se realizaron aislamientos, pruebas bioquímicas y moleculares mediante la amplificación del fragmento 16S DNAr y pruebas de patogenicidad con resultados positivos. El patógeno purificado fue una bacteria Gram positiva; en microscopia electrónica se observaron células en forma de bacilo, con presencia de endosporas en la parte terminal y flagelos peritricos. Creció hasta 50°C en medio de agar nutritivo (AN). Este organismo, se...
Palavras-chave: Patogenicidad; Prueba molecular; Microscopia electrónica; Pathogenicity; Molecular testing; Electron microscopy; Fitopatología; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/1747
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Diversity among satellite glial cells in dorsal root ganglia of the rat BJMBR
Nascimento,R.S.; Santiago,M.F.; Marques,S.A.; Allodi,S.; Martinez,A.M.B..
Peripheral glial cells consist of satellite, enteric glial, and Schwann cells. In dorsal root ganglia, besides pseudo-unipolar neurons, myelinated and nonmyelinated fibers, macrophages, and fibroblasts, satellite cells also constitute the resident components. Information on satellite cells is not abundant; however, they appear to provide mechanical and metabolic support for neurons by forming an envelope surrounding their cell bodies. Although there is a heterogeneous population of neurons in the dorsal root ganglia, satellite cells have been described to be a homogeneous group of perineuronal cells. Our objective was to characterize the ultrastructure, immunohistochemistry, and histochemistry of the satellite cells of the dorsal root ganglia of 17 adult...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Satellite glial cells; Peripheral nervous system; Vimentin; S-100; Electron microscopy.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100011
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Effect of melatonin and time of administration on irradiation-induced damage to rat testes BJMBR
Take,G.; Erdogan,D.; Helvacioglu,F.; Göktas,G.; Ozbey,G.; Uluoglu,C.; Yücel,B.; Guney,Y.; Hicsonmez,A.; Ozkan,S..
The effect of ionizing irradiation on testes and the protective effects of melatonin were investigated by immunohistochemical and electron microscopic methods. Eighty-two adult male Wistar rats were divided into 10 groups. The rats in the irradiated groups were exposed to a sublethal irradiation dose of 8 Gy, either to the total body or abdominopelvic region using a 60Co source at a focus of 80 cm away from the skin in the morning or evening together with vehicle (20% ethanol) or melatonin administered 24 h before (10 mg/kg), immediately before (20 mg/kg) and 24 h after irradiation (10 mg/kg), all ip. Caspace-3 immunoreactivity was increased in the irradiated group compared to control (P < 0.05). Melatonin-treated groups showed less apoptosis as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Testes; Gamma-irradiation; Melatonin; Immunohistochemistry; Electron microscopy.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700006
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Electron microscopy of glial cells of the central nervous system in the crab Ucides cordatus BJMBR
Allodi,S.; Taffarel,M..
Invertebrate glial cells show a variety of morphologies depending on species and location. They have been classified according to relatively general morphological or functional criteria and also to their location. The present study was carried out to characterize the organization of glial cells and their processes in the zona fasciculata and in the protocerebral tract of the crab Ucides cordatus. We performed routine and cytochemical procedures for electron microscopy analysis. Semithin sections were observed at the light microscope. The Thiéry procedure indicated the presence of carbohydrates, particularly glycogen, in tissue and in cells. To better visualize the axonal ensheathment at the ultrastructural level, we employed a method to enhance the...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Glial cells; Crustaceans; Electron microscopy; Cytochemistry; Ucides cordatus.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300013
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Essential oils on the control of stem and ear rot in maize Ciência Rural
Teixeira,Glauco Antonio; Alves,Eduardo; Amaral,Douglas Carvalho; Machado,José da Cruz; Perina,Fabiano José.
Stem and ear rot caused by Stenocarpella maydis are responsible for severe losses in maize production. Treatment of seeds with fungicides may induce environmental damage. Hence, this study aimed to evaluate the effects of essential oils extracted from Cymbopogon winterianus, Thymus vulgaris, Cymbopogon citratus, Corymbia citriodora, Cinnamomum zeylanicum, and Syzygium aromaticum on the development of in vitro S. maydis. In addition, maize seeds were treated with these essential oils to determine their possible mode of action and effects. The oils from S. aromaticum, C. zeylanicum, and T. vulgaris inhibited fungal development at concentrations higher than 0.025%. The oils from S. aromaticum and C. zeylanicum showed seed germination rates of 89.0% and 84.5%,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stenocarpella maydis; Zea mays; Alternative control; Electron microscopy.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001100004
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Etude au microscope electronique a balayage des relations entre bacteries et matieres en suspension en milieu marin ArchiMer
Cormier, M; Bassoulet, P; Pommepuy, Monique.
Near outfall areas numerous bacteria appear associated to colloidal organic matter present in littoral sea water. Arrangements into colonies are shown. Surface structures are observed which allow bacteria's attachment each to other and to organic matter.
Tipo: Text Palavras-chave: Bacteria; Outfalls; Electron microscopy; Flocculation; Colloids; Adhesion; Suspended particulate matter.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-966.pdf
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Evaluation of antibacterial properties of Barium Zirconate Titanate (BZT) nanoparticle BJM
Mohseni,Simin; Aghayan,Mahdi; Ghorani-Azam,Adel; Behdani,Mohammad; Asoodeh,Ahmad.
So far, the antibacterial activity of some organic and inorganic compounds has been studied. Barium zirconate titanate [Ba(Zr xTi1-x)O3] (x = 0.05) nanoparticle is an example of inorganic materials. In vitro studies have provided evidence for the antibacterial activity of this nanoparticle. In the current study, the nano-powder was synthesized by sol-gel method. X-ray diffraction showed that the powder was single-phase and had a perovskite structure at the calcination temperature of 1000 ºC. Antibacterial activity of the desired nanoparticle was assessed on two gram-positive (Staphylococcus aureus PTCC1431 and Micrococcus luteus PTCC1625) and two gram-negative (Escherichia coli HP101BA 7601c and clinically isolated Klebsiella pneumoniae) bacteria according...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanoparticles; Antibiotics; Barium zirconate titanate; Ceramics; Electron microscopy.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400033
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Extracellular breakdown of collagen by mice decidual cells. A cytochemical and ultrastructural study Biocell
Godosevicius Katz,Sima.
The interaction of antimesometrial decidual cells and collagen fibrils was studied by light microscopy and ultrastructural cytochemistry in fed and acutely fasted mice on days 9-11 of pregnancy. Fibrillar elements in the extracellular space consisted of collagen fibrils and filamentous aggregates (disintegrating collagen fibrils). Intracellular vacuoles exhibited typical collagen immersed in electron-translucent material (clear vacuoles) and faint cross-banded collagen immersed in electron-opaque material (dark vacuoles). Fibrillar elements showed extracellular acid phosphatase activity which was stronger in the region of mature decidua than in predecidual cells region in all animals; it was conspicuous in mature decidua of fasted animals. Intracellular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decidua; Acid phosphatase; Fasting; Electron microscopy; Mice.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300003
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